HIPHOP chemogenomics database

YHR185C / PFS1

Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation

Zygosity: Homozygous strain

fixedexpanded
Profile for YHR185C / PFS1

Click on Significant Values for Screen Details

Top fitness defect scores for YHR185C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_143	4032-1194	5.50	15.7 µM	5.01E-8		22	1.89E-8
2	SGTC_934	2950-0684	5.22	338.0 µM	2.11E-7		7	8.72E-8
3	SGTC_155	k072-0202	5.08	43.6 µM	4.47E-7		9	1.94E-7
4	SGTC_96	0929-0093	4.86	37.3 µM	1.24E-6	calcium & mitochondrial duress	6	5.72E-7
5	SGTC_272	doxorubicin	4.82	8.1 µM	1.52E-6	anthracycline transcription coupled DNA repair	9	7.12E-7
6	SGTC_1095	3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran	4.29	15.5 µM	1.61E-5		20	8.74E-6
7	SGTC_2024	5144227	4.19	134.0 µM	2.54E-5		30	1.41E-5
8	SGTC_864	0469-0573	4.09	172.0 µM	3.85E-5		14	2.20E-5
9	SGTC_277	nystatin	3.94	15.5 µM	6.93E-5		13	4.10E-5
10	SGTC_2787	5284540	3.84	71.4 µM	1.03E-4		26	6.23E-5
11	SGTC_198	3454-2873	3.80	16.2 µM	1.19E-4	60S ribosome export	14	7.32E-5
12	SGTC_2502	avocadyne acetate	3.77	18.6 µM	1.32E-4		27	8.10E-5
13	SGTC_932	2857-0803	3.77	154.0 µM	1.34E-4		31	8.29E-5
14	SGTC_2501	sodium tetradecyl sulfate	3.54	13.4 µM	3.09E-4		47	2.01E-4
15	SGTC_262	fenpropimorph	3.51	62.5 µM	3.40E-4	ergosterol depletion effects on membrane	38	2.22E-4
16	SGTC_1072	oxiconazole	3.41	14.9 nM	4.86E-4	ergosterol depletion effects on membrane	69	3.24E-4
17	SGTC_2420	aureobasidin a	3.39	50.0 nM	5.13E-4		67	3.43E-4
18	SGTC_35	0133-0054	3.38	64.1 µM	5.31E-4		51	3.57E-4
19	SGTC_145	3737-0113	3.37	38.4 µM	5.58E-4	DNA intercalators	105	3.75E-4
20	SGTC_114	0364-0001	3.35	490.3 µM	5.96E-4		78	4.03E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.127	1.90E-13	YDR401W_d	YDR401W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.124	6.28E-13	YFL022C	FRS2	het	Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.121	2.33E-12	YDL211C_p	YDL211C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.119	5.28E-12	YIL143C	SSL2	het	Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
0.114	3.92E-11	YMR302C	YME2	hom	Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
0.111	9.89E-11	YDR304C	CPR5	hom	Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER
0.108	4.17E-10	YDL096C_d	OPI6_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.098	1.11E-8	YHR149C	SKG6	hom	Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.097	1.65E-8	YHL039W	EFM1	hom	Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.093	6.25E-8	YDR409W	SIZ1	hom	SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
0.092	8.54E-8	YGR109C	CLB6	hom	B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1
0.092	9.80E-8	YDR257C	RKM4	hom	Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
0.089	2.20E-7	YDL227C	HO	hom	Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p
0.089	2.27E-7	YIL092W_p	YIL092W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
0.086	6.22E-7	YDL122W	UBP1	hom	Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains