YHR185C / PFS1

Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR185C / PFS1

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:3.38|P-value:5.31E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:4.86|P-value:1.24E-6 ID:SGTC_114|Compound:0364-0001|FD-Score:3.35|P-value:5.96E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.25|P-value:8.48E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:5.50|P-value:5.01E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:3.37|P-value:5.58E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.54|P-value:2.86E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:5.08|P-value:4.47E-7 ID:SGTC_188|Compound:3229-0966|FD-Score:3.29|P-value:7.30E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.80|P-value:1.19E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:3.51|P-value:3.40E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:4.82|P-value:1.52E-6 ID:SGTC_277|Compound:nystatin|FD-Score:3.94|P-value:6.93E-5 ID:SGTC_343|Compound:4130-2827|FD-Score:-3.37|P-value:5.16E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:4.09|P-value:3.85E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.77|P-value:1.34E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:5.22|P-value:2.11E-7 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.53|P-value:2.91E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.36|P-value:5.30E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.43|P-value:4.16E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.41|P-value:4.86E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:-3.73|P-value:1.42E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:4.29|P-value:1.61E-5 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.22|P-value:9.28E-4 ID:SGTC_1763|Compound:st044482|FD-Score:-3.41|P-value:4.58E-4 ID:SGTC_2014|Compound:4013930|FD-Score:-3.76|P-value:1.25E-4 ID:SGTC_2024|Compound:5144227|FD-Score:4.19|P-value:2.54E-5 ID:SGTC_2343|Compound:7990716|FD-Score:-3.49|P-value:3.36E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-3.90|P-value:7.54E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.39|P-value:5.13E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:-4.22|P-value:1.98E-5 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:3.54|P-value:3.09E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.77|P-value:1.32E-4 ID:SGTC_2787|Compound:5284540|FD-Score:3.84|P-value:1.03E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.38|P-value:5.31E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:4.86|P-value:1.24E-6 ID:SGTC_114|Compound:0364-0001|FD-Score:3.35|P-value:5.96E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.25|P-value:8.48E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:5.50|P-value:5.01E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:3.37|P-value:5.58E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.54|P-value:2.86E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:5.08|P-value:4.47E-7 ID:SGTC_188|Compound:3229-0966|FD-Score:3.29|P-value:7.30E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.80|P-value:1.19E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:3.51|P-value:3.40E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:4.82|P-value:1.52E-6 ID:SGTC_277|Compound:nystatin|FD-Score:3.94|P-value:6.93E-5 ID:SGTC_343|Compound:4130-2827|FD-Score:-3.37|P-value:5.16E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:4.09|P-value:3.85E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.77|P-value:1.34E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:5.22|P-value:2.11E-7 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.53|P-value:2.91E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.36|P-value:5.30E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.43|P-value:4.16E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.41|P-value:4.86E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:-3.73|P-value:1.42E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:4.29|P-value:1.61E-5 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.22|P-value:9.28E-4 ID:SGTC_1763|Compound:st044482|FD-Score:-3.41|P-value:4.58E-4 ID:SGTC_2014|Compound:4013930|FD-Score:-3.76|P-value:1.25E-4 ID:SGTC_2024|Compound:5144227|FD-Score:4.19|P-value:2.54E-5 ID:SGTC_2343|Compound:7990716|FD-Score:-3.49|P-value:3.36E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-3.90|P-value:7.54E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.39|P-value:5.13E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:-4.22|P-value:1.98E-5 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:3.54|P-value:3.09E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.77|P-value:1.32E-4 ID:SGTC_2787|Compound:5284540|FD-Score:3.84|P-value:1.03E-4

Top fitness defect scores for YHR185C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_143 4032-1194 5.50 15.7 µM 5.01E-8 22 1.89E-8
2 SGTC_934 2950-0684 5.22 338.0 µM 2.11E-7 7 8.72E-8
3 SGTC_155 k072-0202 5.08 43.6 µM 4.47E-7 9 1.94E-7
4 SGTC_96 0929-0093 4.86 37.3 µM 1.24E-6 calcium & mitochondrial duress 6 5.72E-7
5 SGTC_272 doxorubicin 4.82 8.1 µM 1.52E-6 anthracycline transcription coupled DNA repair 9 7.12E-7
6 SGTC_1095 3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran 4.29 15.5 µM 1.61E-5 20 8.74E-6
7 SGTC_2024 5144227 4.19 134.0 µM 2.54E-5 30 1.41E-5
8 SGTC_864 0469-0573 4.09 172.0 µM 3.85E-5 14 2.20E-5
9 SGTC_277 nystatin 3.94 15.5 µM 6.93E-5 13 4.10E-5
10 SGTC_2787 5284540 3.84 71.4 µM 1.03E-4 26 6.23E-5
11 SGTC_198 3454-2873 3.80 16.2 µM 1.19E-4 60S ribosome export 14 7.32E-5
12 SGTC_2502 avocadyne acetate 3.77 18.6 µM 1.32E-4 27 8.10E-5
13 SGTC_932 2857-0803 3.77 154.0 µM 1.34E-4 31 8.29E-5
14 SGTC_2501 sodium tetradecyl sulfate 3.54 13.4 µM 3.09E-4 47 2.01E-4
15 SGTC_262 fenpropimorph 3.51 62.5 µM 3.40E-4 ergosterol depletion effects on membrane 38 2.22E-4
16 SGTC_1072 oxiconazole 3.41 14.9 nM 4.86E-4 ergosterol depletion effects on membrane 69 3.24E-4
17 SGTC_2420 aureobasidin a 3.39 50.0 nM 5.13E-4 67 3.43E-4
18 SGTC_35 0133-0054 3.38 64.1 µM 5.31E-4 51 3.57E-4
19 SGTC_145 3737-0113 3.37 38.4 µM 5.58E-4 DNA intercalators 105 3.75E-4
20 SGTC_114 0364-0001 3.35 490.3 µM 5.96E-4 78 4.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.90E-13 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.124 6.28E-13 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.121 2.33E-12 YDL211C_p YDL211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.119 5.28E-12 YIL143C SSL2 het Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
0.114 3.92E-11 YMR302C YME2 hom Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
0.111 9.89E-11 YDR304C CPR5 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER
0.108 4.17E-10 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.098 1.11E-8 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.097 1.65E-8 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.093 6.25E-8 YDR409W SIZ1 hom SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
0.092 8.54E-8 YGR109C CLB6 hom B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1
0.092 9.80E-8 YDR257C RKM4 hom Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
0.089 2.20E-7 YDL227C HO hom Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p
0.089 2.27E-7 YIL092W_p YIL092W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
0.086 6.22E-7 YDL122W UBP1 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains