YHR187W / IKI1

Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR187W / IKI1

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:4.55|P-value:4.29E-6 ID:SGTC_42|Compound:0133-0086|FD-Score:5.24|P-value:1.55E-7 ID:SGTC_57|Compound:miconazole|FD-Score:-4.18|P-value:2.81E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.02|P-value:6.29E-7 ID:SGTC_73|Compound:0327-0325|FD-Score:-5.47|P-value:6.28E-8 ID:SGTC_173|Compound:1273-0061|FD-Score:-4.57|P-value:5.13E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.76|P-value:1.48E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:3.83|P-value:9.04E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:3.53|P-value:2.73E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.19|P-value:8.83E-4 ID:SGTC_269|Compound:exalamide|FD-Score:4.40|P-value:8.61E-6 ID:SGTC_277|Compound:nystatin|FD-Score:3.82|P-value:9.40E-5 ID:SGTC_306|Compound:1672-3436|FD-Score:3.27|P-value:6.70E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.82|P-value:1.17E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-3.81|P-value:1.23E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:3.26|P-value:7.09E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.60|P-value:2.13E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.60|P-value:3.18E-8 ID:SGTC_456|Compound:gingerol|FD-Score:3.32|P-value:5.70E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-3.36|P-value:6.05E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.79|P-value:1.30E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:4.49|P-value:5.77E-6 ID:SGTC_667|Compound:1498-1044|FD-Score:3.61|P-value:2.05E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:4.02|P-value:4.25E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:5.54|P-value:3.23E-8 ID:SGTC_685|Compound:1278-0033|FD-Score:4.60|P-value:3.49E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:7.56|P-value:8.70E-14 ID:SGTC_704|Compound:1310-0270|FD-Score:5.09|P-value:3.34E-7 ID:SGTC_744|Compound:1594-0068|FD-Score:3.47|P-value:3.42E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.35|P-value:1.04E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:3.23|P-value:7.73E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.54|P-value:2.66E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.43|P-value:7.44E-6 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.61|P-value:2.07E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.20|P-value:1.97E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.47|P-value:3.43E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.21|P-value:8.19E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:-3.23|P-value:9.46E-4 ID:SGTC_1754|Compound:st042065|FD-Score:8.48|P-value:7.18E-17 ID:SGTC_1800|Compound:st050079|FD-Score:5.58|P-value:2.54E-8 ID:SGTC_1803|Compound:st050068|FD-Score:6.78|P-value:1.87E-11 ID:SGTC_1804|Compound:st050076|FD-Score:-3.68|P-value:2.00E-4 ID:SGTC_1818|Compound:st052136|FD-Score:6.12|P-value:1.16E-9 ID:SGTC_1844|Compound:rapamycin|FD-Score:18.60|P-value:1.18E-73 ID:SGTC_1868|Compound:st057644|FD-Score:5.59|P-value:2.40E-8 ID:SGTC_1881|Compound:5652484|FD-Score:3.66|P-value:1.68E-4 ID:SGTC_1945|Compound:st075991|FD-Score:5.32|P-value:1.01E-7 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.61|P-value:2.59E-4 ID:SGTC_2257|Compound:7960413|FD-Score:5.61|P-value:2.15E-8 ID:SGTC_2307|Compound:7538926|FD-Score:3.29|P-value:6.26E-4 ID:SGTC_2325|Compound:6158266|FD-Score:5.15|P-value:2.42E-7 ID:SGTC_2327|Compound:6189656|FD-Score:4.50|P-value:5.42E-6 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-4.40|P-value:1.10E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:15.70|P-value:6.39E-53 ID:SGTC_2771|Compound:fluoxetine|FD-Score:3.95|P-value:5.61E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.89|P-value:8.97E-5 ID:SGTC_2842|Compound:9000675|FD-Score:3.88|P-value:7.31E-5 ID:SGTC_2865|Compound:9030313|FD-Score:3.96|P-value:5.26E-5 ID:SGTC_2869|Compound:9037858|FD-Score:-5.78|P-value:1.22E-8 ID:SGTC_2873|Compound:9040596|FD-Score:5.99|P-value:2.55E-9 ID:SGTC_2896|Compound:9052656|FD-Score:-5.98|P-value:3.70E-9 ID:SGTC_2912|Compound:7964298|FD-Score:4.08|P-value:3.29E-5 ID:SGTC_2915|Compound:7971052|FD-Score:3.76|P-value:1.18E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.53|P-value:2.73E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-4.64|P-value:3.81E-6 ID:SGTC_3202|Compound:9113761|FD-Score:5.31|P-value:1.07E-7 ID:SGTC_3209|Compound:9113616|FD-Score:-3.66|P-value:2.14E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-4.69|P-value:3.01E-6 ID:SGTC_3263|Compound:9137018|FD-Score:4.36|P-value:1.00E-5 ID:SGTC_3308|Compound:9128332|FD-Score:-3.73|P-value:1.63E-4 ID:SGTC_3312|Compound:9133330|FD-Score:3.40|P-value:4.41E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.55|P-value:4.29E-6 ID:SGTC_42|Compound:0133-0086|FD-Score:5.24|P-value:1.55E-7 ID:SGTC_57|Compound:miconazole|FD-Score:-4.18|P-value:2.81E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.02|P-value:6.29E-7 ID:SGTC_73|Compound:0327-0325|FD-Score:-5.47|P-value:6.28E-8 ID:SGTC_173|Compound:1273-0061|FD-Score:-4.57|P-value:5.13E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.76|P-value:1.48E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:3.83|P-value:9.04E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:3.53|P-value:2.73E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.19|P-value:8.83E-4 ID:SGTC_269|Compound:exalamide|FD-Score:4.40|P-value:8.61E-6 ID:SGTC_277|Compound:nystatin|FD-Score:3.82|P-value:9.40E-5 ID:SGTC_306|Compound:1672-3436|FD-Score:3.27|P-value:6.70E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.82|P-value:1.17E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-3.81|P-value:1.23E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:3.26|P-value:7.09E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.60|P-value:2.13E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.60|P-value:3.18E-8 ID:SGTC_456|Compound:gingerol|FD-Score:3.32|P-value:5.70E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-3.36|P-value:6.05E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.79|P-value:1.30E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:4.49|P-value:5.77E-6 ID:SGTC_667|Compound:1498-1044|FD-Score:3.61|P-value:2.05E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:4.02|P-value:4.25E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:5.54|P-value:3.23E-8 ID:SGTC_685|Compound:1278-0033|FD-Score:4.60|P-value:3.49E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:7.56|P-value:8.70E-14 ID:SGTC_704|Compound:1310-0270|FD-Score:5.09|P-value:3.34E-7 ID:SGTC_744|Compound:1594-0068|FD-Score:3.47|P-value:3.42E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.35|P-value:1.04E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:3.23|P-value:7.73E-4 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.54|P-value:2.66E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:4.43|P-value:7.44E-6 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.61|P-value:2.07E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:4.20|P-value:1.97E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.47|P-value:3.43E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.21|P-value:8.19E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:-3.23|P-value:9.46E-4 ID:SGTC_1754|Compound:st042065|FD-Score:8.48|P-value:7.18E-17 ID:SGTC_1800|Compound:st050079|FD-Score:5.58|P-value:2.54E-8 ID:SGTC_1803|Compound:st050068|FD-Score:6.78|P-value:1.87E-11 ID:SGTC_1804|Compound:st050076|FD-Score:-3.68|P-value:2.00E-4 ID:SGTC_1818|Compound:st052136|FD-Score:6.12|P-value:1.16E-9 ID:SGTC_1844|Compound:rapamycin|FD-Score:18.60|P-value:1.18E-73 ID:SGTC_1868|Compound:st057644|FD-Score:5.59|P-value:2.40E-8 ID:SGTC_1881|Compound:5652484|FD-Score:3.66|P-value:1.68E-4 ID:SGTC_1945|Compound:st075991|FD-Score:5.32|P-value:1.01E-7 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.61|P-value:2.59E-4 ID:SGTC_2257|Compound:7960413|FD-Score:5.61|P-value:2.15E-8 ID:SGTC_2307|Compound:7538926|FD-Score:3.29|P-value:6.26E-4 ID:SGTC_2325|Compound:6158266|FD-Score:5.15|P-value:2.42E-7 ID:SGTC_2327|Compound:6189656|FD-Score:4.50|P-value:5.42E-6 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-4.40|P-value:1.10E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:15.70|P-value:6.39E-53 ID:SGTC_2771|Compound:fluoxetine|FD-Score:3.95|P-value:5.61E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.89|P-value:8.97E-5 ID:SGTC_2842|Compound:9000675|FD-Score:3.88|P-value:7.31E-5 ID:SGTC_2865|Compound:9030313|FD-Score:3.96|P-value:5.26E-5 ID:SGTC_2869|Compound:9037858|FD-Score:-5.78|P-value:1.22E-8 ID:SGTC_2873|Compound:9040596|FD-Score:5.99|P-value:2.55E-9 ID:SGTC_2896|Compound:9052656|FD-Score:-5.98|P-value:3.70E-9 ID:SGTC_2912|Compound:7964298|FD-Score:4.08|P-value:3.29E-5 ID:SGTC_2915|Compound:7971052|FD-Score:3.76|P-value:1.18E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.53|P-value:2.73E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-4.64|P-value:3.81E-6 ID:SGTC_3202|Compound:9113761|FD-Score:5.31|P-value:1.07E-7 ID:SGTC_3209|Compound:9113616|FD-Score:-3.66|P-value:2.14E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-4.69|P-value:3.01E-6 ID:SGTC_3263|Compound:9137018|FD-Score:4.36|P-value:1.00E-5 ID:SGTC_3308|Compound:9128332|FD-Score:-3.73|P-value:1.63E-4 ID:SGTC_3312|Compound:9133330|FD-Score:3.40|P-value:4.41E-4

Top fitness defect scores for YHR187W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 18.60 4.0 nM 1.18E-73 6 9.20E-78
2 SGTC_2679 rapamycin 15.70 1.0 nM 6.39E-53 6 8.27E-56
3 SGTC_1754 st042065 8.48 16.1 µM 7.18E-17 NEO1-PIK1 50 1.13E-17
4 SGTC_686 1319-0111 7.56 510.0 µM 8.70E-14 60S ribosome export 1 2.04E-14
5 SGTC_1803 st050068 6.78 52.4 µM 1.87E-11 NEO1-PIK1 60 5.90E-12
6 SGTC_1818 st052136 6.12 56.3 µM 1.16E-9 NEO1-PIK1 12 4.61E-10
7 SGTC_2873 9040596 5.99 71.4 µM 2.55E-9 14 1.06E-9
8 SGTC_2257 7960413 5.61 200.0 µM 2.15E-8 NEO1-PIK1 19 9.98E-9
9 SGTC_1868 st057644 5.59 42.7 µM 2.40E-8 12 1.12E-8
10 SGTC_1800 st050079 5.58 29.1 µM 2.54E-8 NEO1-PIK1 52 1.19E-8
11 SGTC_679 0422-0091 5.54 41.4 µM 3.23E-8 Golgi 9 1.53E-8
12 SGTC_1945 st075991 5.32 56.6 µM 1.01E-7 60S ribosome export 4 5.09E-8
13 SGTC_3202 9113761 5.31 49.5 µM 1.07E-7 17 5.43E-8
14 SGTC_42 0133-0086 5.24 60.6 µM 1.55E-7 6 8.01E-8
15 SGTC_2325 6158266 5.15 200.0 µM 2.42E-7 9 1.28E-7
16 SGTC_704 1310-0270 5.09 142.0 µM 3.34E-7 13 1.79E-7
17 SGTC_685 1278-0033 4.60 54.9 µM 3.49E-6 60S ribosome export 12 2.12E-6
18 SGTC_37 0566-0070 4.55 1.6 µM 4.29E-6 17 2.63E-6
19 SGTC_2327 6189656 4.50 200.0 µM 5.42E-6 14 3.37E-6
20 SGTC_629 3448-6466 4.49 76.7 µM 5.77E-6 8 3.60E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.406 4.90E-133 YGR200C ELP2 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.403 2.37E-131 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.366 7.35E-107 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.352 1.44E-98 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.351 6.10E-98 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.340 2.21E-91 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.328 6.25E-85 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.323 1.76E-82 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.318 9.41E-80 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.316 2.47E-78 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.307 2.10E-74 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.285 7.85E-64 YOR184W SER1 hom 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.265 8.90E-55 YHR111W UBA4 hom Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.255 8.24E-51 YIL008W URM1 hom Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
0.253 3.85E-50 YPL202C AFT2 hom Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication