YHR195W / NVJ1

Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR195W / NVJ1

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:3.71|P-value:1.42E-4 ID:SGTC_26|Compound:0302-0163|FD-Score:3.72|P-value:1.36E-4 ID:SGTC_89|Compound:0302-0250|FD-Score:6.40|P-value:1.55E-10 ID:SGTC_150|Compound:3970-0969|FD-Score:3.64|P-value:1.90E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.51|P-value:3.05E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:3.37|P-value:4.97E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:5.44|P-value:4.61E-8 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.25|P-value:7.49E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.83|P-value:8.83E-5 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.42|P-value:7.35E-6 ID:SGTC_529|Compound:4076-0302|FD-Score:5.03|P-value:4.04E-7 ID:SGTC_538|Compound:2922-0838|FD-Score:3.50|P-value:3.16E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.25|P-value:7.56E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.75|P-value:1.24E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:3.47|P-value:3.46E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.39|P-value:4.74E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.22|P-value:8.32E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.63|P-value:1.97E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:3.98|P-value:5.00E-5 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.37|P-value:4.92E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.50|P-value:3.14E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.37|P-value:5.02E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:4.08|P-value:3.23E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.50|P-value:3.18E-4 ID:SGTC_1442|Compound:4112-3290|FD-Score:3.19|P-value:9.29E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.50|P-value:3.12E-4 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:4.02|P-value:4.18E-5 ID:SGTC_1567|Compound:st069303|FD-Score:8.73|P-value:4.12E-18 ID:SGTC_1602|Compound:st001878|FD-Score:3.30|P-value:6.47E-4 ID:SGTC_1663|Compound:st014140|FD-Score:5.15|P-value:2.19E-7 ID:SGTC_1784|Compound:st039334|FD-Score:6.32|P-value:2.73E-10 ID:SGTC_1807|Compound:st050092|FD-Score:-3.10|P-value:9.42E-4 ID:SGTC_1814|Compound:st052110|FD-Score:3.81|P-value:9.57E-5 ID:SGTC_1884|Compound:5633444|FD-Score:3.73|P-value:1.34E-4 ID:SGTC_1919|Compound:st067113|FD-Score:5.62|P-value:1.74E-8 ID:SGTC_1932|Compound:st074806|FD-Score:-3.83|P-value:6.39E-5 ID:SGTC_1990|Compound:st072050|FD-Score:3.44|P-value:3.84E-4 ID:SGTC_2083|Compound:5306046|FD-Score:5.83|P-value:5.14E-9 ID:SGTC_2254|Compound:7938975|FD-Score:-3.25|P-value:5.53E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.40|P-value:4.42E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.90|P-value:7.58E-7 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:3.55|P-value:2.58E-4 ID:SGTC_2570|Compound:licareol|FD-Score:3.25|P-value:7.63E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.25|P-value:7.46E-4 ID:SGTC_2591|Compound:skatole|FD-Score:-3.20|P-value:6.59E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-4.54|P-value:2.78E-6 ID:SGTC_2644|Compound:guaiazulene|FD-Score:3.48|P-value:3.35E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-4.47|P-value:4.04E-6 ID:SGTC_2878|Compound:9043973|FD-Score:4.56|P-value:3.90E-6 ID:SGTC_2971|Compound:9092186|FD-Score:3.29|P-value:6.63E-4 ID:SGTC_2983|Compound:9022671|FD-Score:-3.26|P-value:5.33E-4 ID:SGTC_3015|Compound:9080845|FD-Score:-3.26|P-value:5.38E-4 ID:SGTC_3045|Compound:9092950|FD-Score:3.20|P-value:8.99E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.57|P-value:2.42E-4 ID:SGTC_3195|Compound:9113082|FD-Score:-3.10|P-value:9.39E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-3.22|P-value:6.07E-4 ID:SGTC_3294|Compound:9122953|FD-Score:3.83|P-value:9.12E-5 ID:SGTC_3341|Compound:9147222|FD-Score:3.94|P-value:5.87E-5 ID:SGTC_22|Compound:0964-0034|FD-Score:3.71|P-value:1.42E-4 ID:SGTC_26|Compound:0302-0163|FD-Score:3.72|P-value:1.36E-4 ID:SGTC_89|Compound:0302-0250|FD-Score:6.40|P-value:1.55E-10 ID:SGTC_150|Compound:3970-0969|FD-Score:3.64|P-value:1.90E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.51|P-value:3.05E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:3.37|P-value:4.97E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:5.44|P-value:4.61E-8 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.25|P-value:7.49E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.83|P-value:8.83E-5 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.42|P-value:7.35E-6 ID:SGTC_529|Compound:4076-0302|FD-Score:5.03|P-value:4.04E-7 ID:SGTC_538|Compound:2922-0838|FD-Score:3.50|P-value:3.16E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.25|P-value:7.56E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.75|P-value:1.24E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:3.47|P-value:3.46E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.39|P-value:4.74E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.22|P-value:8.32E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.63|P-value:1.97E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:3.98|P-value:5.00E-5 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.37|P-value:4.92E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.50|P-value:3.14E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.37|P-value:5.02E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:4.08|P-value:3.23E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.50|P-value:3.18E-4 ID:SGTC_1442|Compound:4112-3290|FD-Score:3.19|P-value:9.29E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.50|P-value:3.12E-4 ID:SGTC_1560|Compound:lithocholic acid|FD-Score:4.02|P-value:4.18E-5 ID:SGTC_1567|Compound:st069303|FD-Score:8.73|P-value:4.12E-18 ID:SGTC_1602|Compound:st001878|FD-Score:3.30|P-value:6.47E-4 ID:SGTC_1663|Compound:st014140|FD-Score:5.15|P-value:2.19E-7 ID:SGTC_1784|Compound:st039334|FD-Score:6.32|P-value:2.73E-10 ID:SGTC_1807|Compound:st050092|FD-Score:-3.10|P-value:9.42E-4 ID:SGTC_1814|Compound:st052110|FD-Score:3.81|P-value:9.57E-5 ID:SGTC_1884|Compound:5633444|FD-Score:3.73|P-value:1.34E-4 ID:SGTC_1919|Compound:st067113|FD-Score:5.62|P-value:1.74E-8 ID:SGTC_1932|Compound:st074806|FD-Score:-3.83|P-value:6.39E-5 ID:SGTC_1990|Compound:st072050|FD-Score:3.44|P-value:3.84E-4 ID:SGTC_2083|Compound:5306046|FD-Score:5.83|P-value:5.14E-9 ID:SGTC_2254|Compound:7938975|FD-Score:-3.25|P-value:5.53E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.40|P-value:4.42E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.90|P-value:7.58E-7 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:3.55|P-value:2.58E-4 ID:SGTC_2570|Compound:licareol|FD-Score:3.25|P-value:7.63E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.25|P-value:7.46E-4 ID:SGTC_2591|Compound:skatole|FD-Score:-3.20|P-value:6.59E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-4.54|P-value:2.78E-6 ID:SGTC_2644|Compound:guaiazulene|FD-Score:3.48|P-value:3.35E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-4.47|P-value:4.04E-6 ID:SGTC_2878|Compound:9043973|FD-Score:4.56|P-value:3.90E-6 ID:SGTC_2971|Compound:9092186|FD-Score:3.29|P-value:6.63E-4 ID:SGTC_2983|Compound:9022671|FD-Score:-3.26|P-value:5.33E-4 ID:SGTC_3015|Compound:9080845|FD-Score:-3.26|P-value:5.38E-4 ID:SGTC_3045|Compound:9092950|FD-Score:3.20|P-value:8.99E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.57|P-value:2.42E-4 ID:SGTC_3195|Compound:9113082|FD-Score:-3.10|P-value:9.39E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-3.22|P-value:6.07E-4 ID:SGTC_3294|Compound:9122953|FD-Score:3.83|P-value:9.12E-5 ID:SGTC_3341|Compound:9147222|FD-Score:3.94|P-value:5.87E-5

Top fitness defect scores for YHR195W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1567 st069303 8.73 68.4 µM 4.12E-18 mitochondrial processes 1 1.26E-18
2 SGTC_89 0302-0250 6.40 142.1 µM 1.55E-10 3 7.55E-11
3 SGTC_1784 st039334 6.32 36.3 µM 2.73E-10 4 1.35E-10
4 SGTC_2083 5306046 5.83 65.9 µM 5.14E-9 1 2.75E-9
5 SGTC_1919 st067113 5.62 52.3 µM 1.74E-8 TSC3-RPN4 11 9.62E-9
6 SGTC_236 1509-0011 5.44 5.3 µM 4.61E-8 304 2.62E-8
7 SGTC_1663 st014140 5.15 55.4 µM 2.19E-7 60S ribosome export 7 1.31E-7
8 SGTC_529 4076-0302 5.03 30.3 µM 4.04E-7 mitochondrial processes 7 2.45E-7
9 SGTC_2552 juarezic acid 4.90 87.0 µM 7.58E-7 TSC3-RPN4 13 4.68E-7
10 SGTC_2878 9043973 4.56 26.0 µM 3.90E-6 8 2.52E-6
11 SGTC_273 hydroxyurea 4.42 18.1 mM 7.35E-6 heme biosynthesis & mitochondrial translocase 24 4.85E-6
12 SGTC_1213 1315-0166 4.08 115.0 µM 3.23E-5 26 2.23E-5
13 SGTC_1560 lithocholic acid 4.02 53.1 µM 4.18E-5 3 2.91E-5
14 SGTC_1002 4049-0206 3.98 172.0 µM 5.00E-5 30 3.50E-5
15 SGTC_3341 9147222 3.94 25.9 µM 5.87E-5 RNA processing & uracil transport 16 4.13E-5
16 SGTC_251 melphalan 3.83 456.5 µM 8.83E-5 DNA damage response 31 6.28E-5
17 SGTC_3294 9122953 3.83 26.3 µM 9.12E-5 tubulin folding & SWR complex 29 6.50E-5
18 SGTC_1814 st052110 3.81 31.5 µM 9.57E-5 RNA processing & uracil transport 12 6.83E-5
19 SGTC_571 k831-0106 3.75 44.2 µM 1.24E-4 18 8.95E-5
20 SGTC_1884 5633444 3.73 25.0 µM 1.34E-4 redox potentiating 62 9.67E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.144 5.78E-17 YPL011C TAF3 het TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
0.137 1.53E-15 YKL109W HAP4 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
0.129 7.90E-14 YAL044W-A_p YAL044W-A_p hom Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein
0.128 1.23E-13 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.127 1.37E-13 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.126 2.41E-13 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.123 9.78E-13 YER141W COX15 hom Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
0.122 1.40E-12 YMR286W MRPL33 hom Mitochondrial ribosomal protein of the large subunit
0.122 1.45E-12 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.121 2.00E-12 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.121 2.30E-12 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.120 2.57E-12 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.116 1.59E-11 YML129C COX14 hom Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes
0.115 2.54E-11 YBR179C FZO1 hom Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
0.114 3.77E-11 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies