YHR204W / MNL1

Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR204W / MNL1

Click on Significant Values for Screen Details ID:SGTC_95|Compound:0471-0001|FD-Score:-3.36|P-value:2.49E-4 ID:SGTC_100|Compound:0086-0128|FD-Score:3.90|P-value:2.85E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:3.70|P-value:6.89E-5 ID:SGTC_292|Compound:k038-0015|FD-Score:3.08|P-value:7.41E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:6.95|P-value:3.54E-13 ID:SGTC_361|Compound:000s-0510|FD-Score:3.32|P-value:3.09E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.81|P-value:4.32E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:7.95|P-value:1.07E-16 ID:SGTC_514|Compound:4466-0038|FD-Score:3.78|P-value:4.76E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:3.31|P-value:3.23E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.75|P-value:5.36E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:3.17|P-value:5.43E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.55|P-value:1.26E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:3.10|P-value:7.00E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:6.71|P-value:2.07E-12 ID:SGTC_951|Compound:1171-0579|FD-Score:3.17|P-value:5.41E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.46|P-value:1.79E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:3.18|P-value:5.29E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.50|P-value:1.52E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:5.14|P-value:5.74E-8 ID:SGTC_1060|Compound:3448-7350|FD-Score:4.95|P-value:1.65E-7 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.88|P-value:2.35E-7 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.73|P-value:5.92E-5 ID:SGTC_1392|Compound:3455-1037|FD-Score:4.09|P-value:1.24E-5 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.38|P-value:2.48E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:4.47|P-value:1.96E-6 ID:SGTC_1456|Compound:k015-0012|FD-Score:5.09|P-value:7.36E-8 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.53|P-value:1.36E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.93|P-value:2.56E-5 ID:SGTC_1508|Compound:1080-0625|FD-Score:4.01|P-value:1.74E-5 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.39|P-value:2.40E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.22|P-value:4.48E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:3.33|P-value:2.98E-4 ID:SGTC_1703|Compound:st026983|FD-Score:-4.46|P-value:1.86E-6 ID:SGTC_1766|Compound:st045414|FD-Score:3.13|P-value:6.20E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.22|P-value:6.58E-6 ID:SGTC_1800|Compound:st050079|FD-Score:4.33|P-value:3.87E-6 ID:SGTC_1801|Compound:st050089|FD-Score:6.02|P-value:2.49E-10 ID:SGTC_1948|Compound:st076329|FD-Score:-3.55|P-value:1.16E-4 ID:SGTC_1959|Compound:st077091|FD-Score:4.13|P-value:1.03E-5 ID:SGTC_2083|Compound:5306046|FD-Score:3.29|P-value:3.42E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:4.73|P-value:5.35E-7 ID:SGTC_2149|Compound:5554786|FD-Score:3.69|P-value:7.10E-5 ID:SGTC_2244|Compound:6635614|FD-Score:-3.67|P-value:7.20E-5 ID:SGTC_2246|Compound:7293507|FD-Score:3.10|P-value:6.91E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.05|P-value:8.45E-4 ID:SGTC_2435|Compound:5706476|FD-Score:4.74|P-value:5.02E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.48|P-value:7.47E-9 ID:SGTC_2584|Compound:androsterone|FD-Score:3.73|P-value:5.86E-5 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.66|P-value:7.48E-5 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.20|P-value:4.87E-4 ID:SGTC_2710|Compound:st078562|FD-Score:4.10|P-value:1.19E-5 ID:SGTC_2715|Compound:st079500|FD-Score:3.20|P-value:4.79E-4 ID:SGTC_2809|Compound:7964133|FD-Score:5.00|P-value:1.20E-7 ID:SGTC_2811|Compound:7967822|FD-Score:3.97|P-value:2.06E-5 ID:SGTC_2818|Compound:7996756|FD-Score:3.36|P-value:2.61E-4 ID:SGTC_2907|Compound:7721985|FD-Score:-3.65|P-value:7.69E-5 ID:SGTC_2993|Compound:9054257|FD-Score:4.34|P-value:3.79E-6 ID:SGTC_3047|Compound:9092122|FD-Score:3.17|P-value:5.46E-4 ID:SGTC_3056|Compound:9094900|FD-Score:4.49|P-value:1.78E-6 ID:SGTC_3070|Compound:9117005|FD-Score:3.21|P-value:4.74E-4 ID:SGTC_3112|Compound:9123419|FD-Score:4.47|P-value:1.99E-6 ID:SGTC_3155|Compound:9099265|FD-Score:3.06|P-value:7.95E-4 ID:SGTC_3174|Compound:9107204|FD-Score:3.94|P-value:2.39E-5 ID:SGTC_3202|Compound:9113761|FD-Score:4.10|P-value:1.18E-5 ID:SGTC_3212|Compound:9128307|FD-Score:3.97|P-value:2.12E-5 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.36|P-value:2.49E-4 ID:SGTC_100|Compound:0086-0128|FD-Score:3.90|P-value:2.85E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:3.70|P-value:6.89E-5 ID:SGTC_292|Compound:k038-0015|FD-Score:3.08|P-value:7.41E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:6.95|P-value:3.54E-13 ID:SGTC_361|Compound:000s-0510|FD-Score:3.32|P-value:3.09E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.81|P-value:4.32E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:7.95|P-value:1.07E-16 ID:SGTC_514|Compound:4466-0038|FD-Score:3.78|P-value:4.76E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:3.31|P-value:3.23E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:3.75|P-value:5.36E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:3.17|P-value:5.43E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:3.55|P-value:1.26E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:3.10|P-value:7.00E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:6.71|P-value:2.07E-12 ID:SGTC_951|Compound:1171-0579|FD-Score:3.17|P-value:5.41E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.46|P-value:1.79E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:3.18|P-value:5.29E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.50|P-value:1.52E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:5.14|P-value:5.74E-8 ID:SGTC_1060|Compound:3448-7350|FD-Score:4.95|P-value:1.65E-7 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.88|P-value:2.35E-7 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.73|P-value:5.92E-5 ID:SGTC_1392|Compound:3455-1037|FD-Score:4.09|P-value:1.24E-5 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.38|P-value:2.48E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:4.47|P-value:1.96E-6 ID:SGTC_1456|Compound:k015-0012|FD-Score:5.09|P-value:7.36E-8 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.53|P-value:1.36E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.93|P-value:2.56E-5 ID:SGTC_1508|Compound:1080-0625|FD-Score:4.01|P-value:1.74E-5 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.39|P-value:2.40E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.22|P-value:4.48E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:3.33|P-value:2.98E-4 ID:SGTC_1703|Compound:st026983|FD-Score:-4.46|P-value:1.86E-6 ID:SGTC_1766|Compound:st045414|FD-Score:3.13|P-value:6.20E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.22|P-value:6.58E-6 ID:SGTC_1800|Compound:st050079|FD-Score:4.33|P-value:3.87E-6 ID:SGTC_1801|Compound:st050089|FD-Score:6.02|P-value:2.49E-10 ID:SGTC_1948|Compound:st076329|FD-Score:-3.55|P-value:1.16E-4 ID:SGTC_1959|Compound:st077091|FD-Score:4.13|P-value:1.03E-5 ID:SGTC_2083|Compound:5306046|FD-Score:3.29|P-value:3.42E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:4.73|P-value:5.35E-7 ID:SGTC_2149|Compound:5554786|FD-Score:3.69|P-value:7.10E-5 ID:SGTC_2244|Compound:6635614|FD-Score:-3.67|P-value:7.20E-5 ID:SGTC_2246|Compound:7293507|FD-Score:3.10|P-value:6.91E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.05|P-value:8.45E-4 ID:SGTC_2435|Compound:5706476|FD-Score:4.74|P-value:5.02E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.48|P-value:7.47E-9 ID:SGTC_2584|Compound:androsterone|FD-Score:3.73|P-value:5.86E-5 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.66|P-value:7.48E-5 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.20|P-value:4.87E-4 ID:SGTC_2710|Compound:st078562|FD-Score:4.10|P-value:1.19E-5 ID:SGTC_2715|Compound:st079500|FD-Score:3.20|P-value:4.79E-4 ID:SGTC_2809|Compound:7964133|FD-Score:5.00|P-value:1.20E-7 ID:SGTC_2811|Compound:7967822|FD-Score:3.97|P-value:2.06E-5 ID:SGTC_2818|Compound:7996756|FD-Score:3.36|P-value:2.61E-4 ID:SGTC_2907|Compound:7721985|FD-Score:-3.65|P-value:7.69E-5 ID:SGTC_2993|Compound:9054257|FD-Score:4.34|P-value:3.79E-6 ID:SGTC_3047|Compound:9092122|FD-Score:3.17|P-value:5.46E-4 ID:SGTC_3056|Compound:9094900|FD-Score:4.49|P-value:1.78E-6 ID:SGTC_3070|Compound:9117005|FD-Score:3.21|P-value:4.74E-4 ID:SGTC_3112|Compound:9123419|FD-Score:4.47|P-value:1.99E-6 ID:SGTC_3155|Compound:9099265|FD-Score:3.06|P-value:7.95E-4 ID:SGTC_3174|Compound:9107204|FD-Score:3.94|P-value:2.39E-5 ID:SGTC_3202|Compound:9113761|FD-Score:4.10|P-value:1.18E-5 ID:SGTC_3212|Compound:9128307|FD-Score:3.97|P-value:2.12E-5

Top fitness defect scores for YHR204W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_453 enantio-paf c16 7.95 10.0 µM 1.07E-16 14 9.18E-16
2 SGTC_351 caspofungin 6.95 25.0 nM 3.54E-13 cell wall 27 1.83E-12
3 SGTC_882 0843-0131 6.71 351.0 µM 2.07E-12 3 9.58E-12
4 SGTC_1801 st050089 6.02 28.4 µM 2.49E-10 NEO1-PIK1 48 8.55E-10
5 SGTC_2582 agaric acid 5.48 100.0 µM 7.47E-9 redox potentiating 189 2.07E-8
6 SGTC_1050 3232-1156 5.14 69.3 µM 5.74E-8 RSC complex & mRNA processing 8 1.41E-7
7 SGTC_1456 k015-0012 5.09 13.0 µM 7.36E-8 10 1.77E-7
8 SGTC_2809 7964133 5.00 13.0 µM 1.20E-7 60S ribosome export 15 2.81E-7
9 SGTC_1060 3448-7350 4.95 82.0 µM 1.65E-7 Golgi 20 3.78E-7
10 SGTC_1179 1021-1431 4.88 9.3 µM 2.35E-7 20 5.28E-7
11 SGTC_2435 5706476 4.74 10.6 µM 5.02E-7 15 1.08E-6
12 SGTC_2131 toluylene red 4.73 5.3 µM 5.35E-7 60S ribosome export 7 1.14E-6
13 SGTC_3056 9094900 4.49 49.5 µM 1.78E-6 10 3.53E-6
14 SGTC_1400 3771-8998 4.47 51.5 µM 1.96E-6 4 3.86E-6
15 SGTC_3112 9123419 4.47 49.5 µM 1.99E-6 RPP1 & pyrimidine depletion 4 3.92E-6
16 SGTC_2993 9054257 4.34 71.4 µM 3.79E-6 fatty acid desaturase (OLE1) 34 7.19E-6
17 SGTC_1800 st050079 4.33 29.1 µM 3.87E-6 NEO1-PIK1 107 7.32E-6
18 SGTC_1785 bathophenanthroline 4.22 24.0 µM 6.58E-6 heme requiring 37 1.21E-5
19 SGTC_1959 st077091 4.13 55.8 µM 1.03E-5 RPP1 & pyrimidine depletion 41 1.84E-5
20 SGTC_3202 9113761 4.10 49.5 µM 1.18E-5 44 2.09E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.200 1.47E-31 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.191 4.72E-29 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.179 1.38E-25 YGL027C CWH41 hom Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
0.171 1.85E-23 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.168 1.18E-22 YLR330W CHS5 hom Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane
0.163 2.52E-21 YBR134W_d YBR134W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.159 2.60E-20 YDR435C PPM1 hom Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
0.158 3.51E-20 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.155 1.90E-19 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.146 1.88E-17 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.144 4.06E-17 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
0.144 6.09E-17 YOR050C_d YOR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.128 8.43E-14 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.127 1.51E-13 YMR214W SCJ1 hom One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
0.124 4.72E-13 YDL134C PPH21 hom Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication