YIL007C / NAS2

Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL007C / NAS2

Click on Significant Values for Screen Details ID:SGTC_27|Compound:0485-0001|FD-Score:2.87|P-value:8.99E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:3.30|P-value:1.72E-4 ID:SGTC_78|Compound:0336-0123|FD-Score:3.16|P-value:2.99E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:6.22|P-value:9.89E-12 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.16|P-value:4.20E-4 ID:SGTC_220|Compound:paclitaxel|FD-Score:-3.58|P-value:7.61E-5 ID:SGTC_338|Compound:4055-0136|FD-Score:3.14|P-value:3.28E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:3.33|P-value:1.50E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.18|P-value:2.75E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:-3.47|P-value:1.19E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:3.91|P-value:1.17E-5 ID:SGTC_901|Compound:1112-0062|FD-Score:2.93|P-value:7.20E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.66|P-value:3.59E-5 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.49|P-value:7.68E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.27|P-value:1.94E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.01|P-value:5.41E-4 ID:SGTC_1444|Compound:4112-3581|FD-Score:4.12|P-value:4.04E-6 ID:SGTC_1602|Compound:st001878|FD-Score:-2.99|P-value:7.94E-4 ID:SGTC_1616|Compound:st002734|FD-Score:4.59|P-value:3.50E-7 ID:SGTC_1621|Compound:st003705|FD-Score:-3.61|P-value:6.51E-5 ID:SGTC_1644|Compound:st010134|FD-Score:3.68|P-value:3.34E-5 ID:SGTC_1757|Compound:st041620|FD-Score:2.87|P-value:8.89E-4 ID:SGTC_1845|Compound:st056753|FD-Score:2.90|P-value:8.07E-4 ID:SGTC_2063|Compound:5246145|FD-Score:5.39|P-value:3.01E-9 ID:SGTC_2133|Compound:5320837|FD-Score:3.36|P-value:1.34E-4 ID:SGTC_2179|Compound:6298986|FD-Score:3.78|P-value:2.09E-5 ID:SGTC_2284|Compound:7945075|FD-Score:3.43|P-value:9.87E-5 ID:SGTC_2534|Compound:helenine|FD-Score:3.18|P-value:2.83E-4 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.22|P-value:2.34E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.61|P-value:3.12E-7 ID:SGTC_2792|Compound:7535235|FD-Score:2.92|P-value:7.46E-4 ID:SGTC_2929|Compound:9013348|FD-Score:-3.19|P-value:3.80E-4 ID:SGTC_3056|Compound:9094900|FD-Score:2.86|P-value:9.49E-4 ID:SGTC_3122|Compound:9126309|FD-Score:2.94|P-value:6.97E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.01|P-value:5.34E-4 ID:SGTC_3272|Compound:9137413|FD-Score:-3.25|P-value:2.95E-4 ID:SGTC_3276|Compound:9139060|FD-Score:3.32|P-value:1.57E-4 ID:SGTC_27|Compound:0485-0001|FD-Score:2.87|P-value:8.99E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:3.30|P-value:1.72E-4 ID:SGTC_78|Compound:0336-0123|FD-Score:3.16|P-value:2.99E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:6.22|P-value:9.89E-12 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.16|P-value:4.20E-4 ID:SGTC_220|Compound:paclitaxel|FD-Score:-3.58|P-value:7.61E-5 ID:SGTC_338|Compound:4055-0136|FD-Score:3.14|P-value:3.28E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:3.33|P-value:1.50E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.18|P-value:2.75E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:-3.47|P-value:1.19E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:3.91|P-value:1.17E-5 ID:SGTC_901|Compound:1112-0062|FD-Score:2.93|P-value:7.20E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.66|P-value:3.59E-5 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.49|P-value:7.68E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.27|P-value:1.94E-4 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.01|P-value:5.41E-4 ID:SGTC_1444|Compound:4112-3581|FD-Score:4.12|P-value:4.04E-6 ID:SGTC_1602|Compound:st001878|FD-Score:-2.99|P-value:7.94E-4 ID:SGTC_1616|Compound:st002734|FD-Score:4.59|P-value:3.50E-7 ID:SGTC_1621|Compound:st003705|FD-Score:-3.61|P-value:6.51E-5 ID:SGTC_1644|Compound:st010134|FD-Score:3.68|P-value:3.34E-5 ID:SGTC_1757|Compound:st041620|FD-Score:2.87|P-value:8.89E-4 ID:SGTC_1845|Compound:st056753|FD-Score:2.90|P-value:8.07E-4 ID:SGTC_2063|Compound:5246145|FD-Score:5.39|P-value:3.01E-9 ID:SGTC_2133|Compound:5320837|FD-Score:3.36|P-value:1.34E-4 ID:SGTC_2179|Compound:6298986|FD-Score:3.78|P-value:2.09E-5 ID:SGTC_2284|Compound:7945075|FD-Score:3.43|P-value:9.87E-5 ID:SGTC_2534|Compound:helenine|FD-Score:3.18|P-value:2.83E-4 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.22|P-value:2.34E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.61|P-value:3.12E-7 ID:SGTC_2792|Compound:7535235|FD-Score:2.92|P-value:7.46E-4 ID:SGTC_2929|Compound:9013348|FD-Score:-3.19|P-value:3.80E-4 ID:SGTC_3056|Compound:9094900|FD-Score:2.86|P-value:9.49E-4 ID:SGTC_3122|Compound:9126309|FD-Score:2.94|P-value:6.97E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.01|P-value:5.34E-4 ID:SGTC_3272|Compound:9137413|FD-Score:-3.25|P-value:2.95E-4 ID:SGTC_3276|Compound:9139060|FD-Score:3.32|P-value:1.57E-4

Top fitness defect scores for YIL007C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_147 4466-0538 6.22 29.2 µM 9.89E-12 ergosterol biosynthesis 3 2.50E-10
2 SGTC_2063 5246145 5.39 200.0 µM 3.01E-9 8 3.58E-8
3 SGTC_2598 perillyl alcohol 4.61 100.0 µM 3.12E-7 17 2.01E-6
4 SGTC_1616 st002734 4.59 56.1 µM 3.50E-7 13 2.23E-6
5 SGTC_1444 4112-3581 4.12 28.0 µM 4.04E-6 7 1.86E-5
6 SGTC_876 0720-0963 3.91 56.8 µM 1.17E-5 16 4.68E-5
7 SGTC_2179 6298986 3.78 200.0 µM 2.09E-5 heme requiring 20 7.75E-5
8 SGTC_1644 st010134 3.68 20.9 µM 3.34E-5 mitochondrial processes 14 1.17E-4
9 SGTC_1146 3579-1023 3.66 7.0 µM 3.59E-5 23 1.24E-4
10 SGTC_1155 4088-0394 3.49 48.2 µM 7.68E-5 48 2.41E-4
11 SGTC_2284 7945075 3.43 39.2 µM 9.87E-5 mitochondrial processes 43 2.99E-4
12 SGTC_2133 5320837 3.36 200.0 µM 1.34E-4 ERG2 48 3.89E-4
13 SGTC_369 0957-0393 3.33 4.5 µM 1.50E-4 cell wall signaling 52 4.31E-4
14 SGTC_3276 9139060 3.32 49.5 µM 1.57E-4 60S ribosome export 14 4.48E-4
15 SGTC_68 0986-0284 3.30 82.8 µM 1.72E-4 105 4.85E-4
16 SGTC_1242 0402-0103 3.27 60.1 µM 1.94E-4 49 5.37E-4
17 SGTC_2559 tetrahydrosappanone a trimethyl ether 3.22 100.0 µM 2.34E-4 32 6.33E-4
18 SGTC_796 1234-0020 3.18 70.7 µM 2.75E-4 SWF1 & branched chain AA biosynthesis 49 7.28E-4
19 SGTC_2534 helenine 3.18 20.1 µM 2.83E-4 RSC complex & mRNA processing 31 7.47E-4
20 SGTC_78 0336-0123 3.16 156.8 µM 2.99E-4 45 7.83E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.121 1.87E-12 YIR013C GAT4 hom Protein containing GATA family zinc finger motifs
0.110 1.64E-10 YGL248W PDE1 hom Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation
0.102 2.72E-9 YDR329C PEX3 hom Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p
0.082 2.00E-6 YIL023C YKE4 hom Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family
0.081 2.43E-6 YHR085W IPI1 het Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
0.080 3.20E-6 YJL208C NUC1 hom Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy
0.079 5.00E-6 YLL019C KNS1 hom Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
0.077 7.01E-6 YER089C PTC2 hom Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
0.075 1.46E-5 YLR249W YEF3 het Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
0.074 1.80E-5 YNR025C_d YNR025C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase
0.072 2.75E-5 YLR403W SFP1 hom Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
0.071 4.00E-5 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.071 4.16E-5 YER039C HVG1 hom Protein of unknown function, has homology to Vrg4p
0.070 4.85E-5 YER020W GPA2 hom Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.068 7.31E-5 YPR041W TIF5 het Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2