YIL015W / BAR1

Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL015W / BAR1

Click on Significant Values for Screen Details ID:SGTC_163|Compound:1327-0036|FD-Score:-3.21|P-value:9.27E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.20|P-value:2.05E-5 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:-4.02|P-value:4.81E-5 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.67|P-value:1.66E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.94|P-value:5.84E-5 ID:SGTC_573|Compound:3702-0553|FD-Score:3.89|P-value:7.21E-5 ID:SGTC_1123|Compound:mangostin|FD-Score:3.51|P-value:2.99E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.75|P-value:1.22E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.19|P-value:9.06E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.79|P-value:1.06E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:5.77|P-value:9.03E-9 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.38|P-value:4.70E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.35|P-value:5.20E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid|FD-Score:3.55|P-value:2.58E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.28|P-value:6.76E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.21|P-value:8.47E-4 ID:SGTC_1739|Compound:st037196|FD-Score:4.05|P-value:3.80E-5 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:4.42|P-value:8.04E-6 ID:SGTC_2327|Compound:6189656|FD-Score:-3.70|P-value:1.66E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.71|P-value:1.42E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:3.93|P-value:6.27E-5 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.38|P-value:4.72E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.85|P-value:5.74E-9 ID:SGTC_2953|Compound:9074162|FD-Score:-3.43|P-value:4.34E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-3.98|P-value:5.57E-5 ID:SGTC_3016|Compound:9080866|FD-Score:3.45|P-value:3.75E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.86|P-value:7.98E-5 ID:SGTC_3032|Compound:9089427|FD-Score:3.93|P-value:6.06E-5 ID:SGTC_3088|Compound:9117578|FD-Score:4.44|P-value:7.19E-6 ID:SGTC_3093|Compound:9117344|FD-Score:3.24|P-value:7.74E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.97|P-value:5.86E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.21|P-value:9.27E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.20|P-value:2.05E-5 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:-4.02|P-value:4.81E-5 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.67|P-value:1.66E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.94|P-value:5.84E-5 ID:SGTC_573|Compound:3702-0553|FD-Score:3.89|P-value:7.21E-5 ID:SGTC_1123|Compound:mangostin|FD-Score:3.51|P-value:2.99E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.75|P-value:1.22E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.19|P-value:9.06E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.79|P-value:1.06E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:5.77|P-value:9.03E-9 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.38|P-value:4.70E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.35|P-value:5.20E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid|FD-Score:3.55|P-value:2.58E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:3.28|P-value:6.76E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.21|P-value:8.47E-4 ID:SGTC_1739|Compound:st037196|FD-Score:4.05|P-value:3.80E-5 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:4.42|P-value:8.04E-6 ID:SGTC_2327|Compound:6189656|FD-Score:-3.70|P-value:1.66E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.71|P-value:1.42E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:3.93|P-value:6.27E-5 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.38|P-value:4.72E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.85|P-value:5.74E-9 ID:SGTC_2953|Compound:9074162|FD-Score:-3.43|P-value:4.34E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-3.98|P-value:5.57E-5 ID:SGTC_3016|Compound:9080866|FD-Score:3.45|P-value:3.75E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.86|P-value:7.98E-5 ID:SGTC_3032|Compound:9089427|FD-Score:3.93|P-value:6.06E-5 ID:SGTC_3088|Compound:9117578|FD-Score:4.44|P-value:7.19E-6 ID:SGTC_3093|Compound:9117344|FD-Score:3.24|P-value:7.74E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.97|P-value:5.86E-5

Top fitness defect scores for YIL015W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2679 rapamycin 5.85 1.0 nM 5.74E-9 80 2.47E-9
2 SGTC_1495 4469-0029 5.77 237.0 µM 9.03E-9 57 3.99E-9
3 SGTC_3088 9117578 4.44 49.5 µM 7.19E-6 amide catabolism 5 4.45E-6
4 SGTC_2039 Aminomethylbenzodioxan 4.42 200.0 µM 8.04E-6 3 5.01E-6
5 SGTC_367 0335-0881 4.20 7.1 µM 2.05E-5 ubiquinone biosynthesis & proteasome 65 1.33E-5
6 SGTC_1739 st037196 4.05 92.5 µM 3.80E-5 18 2.56E-5
7 SGTC_480 am 92016 3.94 50.0 µM 5.84E-5 13 4.01E-5
8 SGTC_3032 9089427 3.93 49.5 µM 6.06E-5 60S ribosome export 17 4.17E-5
9 SGTC_2513 baicalein 3.93 25.6 µM 6.27E-5 calcium & mitochondrial duress 29 4.32E-5
10 SGTC_573 3702-0553 3.89 84.7 µM 7.21E-5 15 5.00E-5
11 SGTC_3031 9088762 3.86 49.5 µM 7.98E-5 23 5.56E-5
12 SGTC_1441 4106-0040 3.79 13.0 µM 1.06E-4 13 7.46E-5
13 SGTC_1222 0366-0074 3.75 1.2 µM 1.22E-4 TSC3-RPN4 21 8.70E-5
14 SGTC_2363 9053361 3.71 176.0 µM 1.42E-4 Golgi 37 1.02E-4
15 SGTC_419 nsc-638432 3.67 893.0 µM 1.66E-4 DNA damage response 15 1.20E-4
16 SGTC_1536 cytidine-2',3'-monophosphoric acid; 3'-cytidylic acid 3.55 61.9 µM 2.58E-4 30 1.90E-4
17 SGTC_1123 mangostin 3.51 811.3 nM 2.99E-4 mitochondrial response to ROS 53 2.22E-4
18 SGTC_3016 9080866 3.45 49.5 µM 3.75E-4 41 2.82E-4
19 SGTC_1514 1189-1591 3.38 158.0 µM 4.70E-4 65 3.56E-4
20 SGTC_2662 daunorubicin 3.38 22.4 µM 4.72E-4 anthracycline transcription coupled DNA repair 30 3.59E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.93E-13 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.124 5.76E-13 YNL007C SIS1 het Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
0.117 8.80E-12 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.104 1.56E-9 YBR077C SLM4 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
0.097 1.82E-8 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.097 1.97E-8 YPL175W SPT14 het UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
0.094 4.23E-8 YKR035W-A DID2 hom Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.090 1.67E-7 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.090 2.00E-7 YOL129W VPS68 hom Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria
0.088 3.56E-7 YLR449W FPR4 hom Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones
0.087 4.50E-7 YGR200C ELP2 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.085 8.20E-7 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.084 1.04E-6 YMR312W ELP6 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.083 1.35E-6 YML100W TSL1 hom Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
0.082 1.73E-6 YBL047C EDE1 hom Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death