YIL021W / RPB3

RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit

Zygosity: Heterozygous strain
fixedexpanded
Profile for YIL021W / RPB3

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:5.67|P-value:1.18E-6 ID:SGTC_32|Compound:0928-0142|FD-Score:4.18|P-value:2.51E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.85|P-value:6.76E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:4.22|P-value:2.27E-4 ID:SGTC_269|Compound:exalamide|FD-Score:3.75|P-value:9.14E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:6.20|P-value:1.25E-7 ID:SGTC_351|Compound:caspofungin|FD-Score:4.53|P-value:8.25E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:4.20|P-value:2.42E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.79|P-value:8.07E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:5.13|P-value:9.85E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:5.43|P-value:3.12E-6 ID:SGTC_549|Compound:1661-1316|FD-Score:3.87|P-value:6.44E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:3.74|P-value:9.27E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:5.72|P-value:9.60E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:4.69|P-value:4.78E-5 ID:SGTC_631|Compound:k072-0230|FD-Score:5.66|P-value:1.27E-6 ID:SGTC_666|Compound:0272-0154|FD-Score:3.73|P-value:9.53E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.09|P-value:3.35E-4 ID:SGTC_720|Compound:r052-1644|FD-Score:-4.07|P-value:3.36E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:3.81|P-value:7.75E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:4.26|P-value:2.01E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:6.34|P-value:6.64E-8 ID:SGTC_885|Compound:0865-0106|FD-Score:4.74|P-value:4.10E-5 ID:SGTC_895|Compound:0964-0006|FD-Score:6.62|P-value:1.82E-8 ID:SGTC_951|Compound:1171-0579|FD-Score:4.02|P-value:4.17E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:5.71|P-value:1.02E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:4.38|P-value:1.34E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:4.13|P-value:2.97E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:5.60|P-value:1.58E-6 ID:SGTC_1224|Compound:0823-0416|FD-Score:4.10|P-value:3.30E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.91|P-value:5.72E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.86|P-value:6.63E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:6.11|P-value:1.85E-7 ID:SGTC_1474|Compound:0330-0125|FD-Score:3.93|P-value:5.49E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.73|P-value:9.66E-4 ID:SGTC_1740|Compound:st038286|FD-Score:4.01|P-value:4.33E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.75|P-value:9.16E-4 ID:SGTC_1774|Compound:st048371|FD-Score:5.13|P-value:9.97E-6 ID:SGTC_1806|Compound:st050005|FD-Score:4.08|P-value:3.42E-4 ID:SGTC_1815|Compound:st051655|FD-Score:4.52|P-value:8.43E-5 ID:SGTC_1816|Compound:st051957|FD-Score:3.76|P-value:8.91E-4 ID:SGTC_1826|Compound:st055655|FD-Score:7.32|P-value:5.52E-10 ID:SGTC_1845|Compound:st056753|FD-Score:8.19|P-value:4.79E-12 ID:SGTC_2013|Compound:4024628|FD-Score:-4.12|P-value:2.95E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-4.87|P-value:2.39E-5 ID:SGTC_2016|Compound:5121758|FD-Score:5.95|P-value:3.69E-7 ID:SGTC_2023|Compound:5144124|FD-Score:-4.37|P-value:1.31E-4 ID:SGTC_2041|Compound:5107442|FD-Score:5.33|P-value:4.60E-6 ID:SGTC_2135|Compound:5326629|FD-Score:4.13|P-value:3.01E-4 ID:SGTC_2163|Compound:5671233|FD-Score:5.49|P-value:2.48E-6 ID:SGTC_2248|Compound:tofa|FD-Score:-4.09|P-value:3.24E-4 ID:SGTC_2254|Compound:7938975|FD-Score:-4.02|P-value:4.00E-4 ID:SGTC_2261|Compound:9010538|FD-Score:-6.19|P-value:1.22E-7 ID:SGTC_2277|Compound:7947813|FD-Score:4.76|P-value:3.72E-5 ID:SGTC_2311|Compound:7694451|FD-Score:4.89|P-value:2.35E-5 ID:SGTC_2336|Compound:9001819|FD-Score:5.06|P-value:1.29E-5 ID:SGTC_2378|Compound:9074213|FD-Score:7.08|P-value:1.89E-9 ID:SGTC_2381|Compound:9035592|FD-Score:4.52|P-value:8.64E-5 ID:SGTC_2417|Compound:cerulenin|FD-Score:3.82|P-value:7.50E-4 ID:SGTC_2470|Compound:5664817|FD-Score:4.54|P-value:8.08E-5 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:4.36|P-value:1.43E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:4.05|P-value:3.84E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.12|P-value:3.04E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.99|P-value:4.58E-4 ID:SGTC_2714|Compound:st079313|FD-Score:4.67|P-value:5.13E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.85|P-value:2.72E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:5.31|P-value:4.96E-6 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:6.00|P-value:2.98E-7 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-5.59|P-value:1.59E-6 ID:SGTC_2779|Compound:methotrexate|FD-Score:-5.38|P-value:3.59E-6 ID:SGTC_2798|Compound:7813637|FD-Score:3.79|P-value:8.15E-4 ID:SGTC_2819|Compound:7996655|FD-Score:4.59|P-value:6.68E-5 ID:SGTC_2851|Compound:9015922|FD-Score:-3.82|P-value:7.17E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-4.00|P-value:4.20E-4 ID:SGTC_2985|Compound:9022375|FD-Score:7.87|P-value:2.80E-11 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.86|P-value:6.71E-4 ID:SGTC_3064|Compound:1327-0027|FD-Score:5.16|P-value:8.88E-6 ID:SGTC_3126|Compound:9127922|FD-Score:-4.34|P-value:1.45E-4 ID:SGTC_3131|Compound:9127911|FD-Score:-4.11|P-value:3.01E-4 ID:SGTC_3140|Compound:9094243|FD-Score:4.86|P-value:2.66E-5 ID:SGTC_3277|Compound:9138724|FD-Score:4.99|P-value:1.65E-5 ID:SGTC_3337|Compound:9144342|FD-Score:3.92|P-value:5.54E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.88|P-value:6.27E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:5.67|P-value:1.18E-6 ID:SGTC_32|Compound:0928-0142|FD-Score:4.18|P-value:2.51E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.85|P-value:6.76E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:4.22|P-value:2.27E-4 ID:SGTC_269|Compound:exalamide|FD-Score:3.75|P-value:9.14E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:6.20|P-value:1.25E-7 ID:SGTC_351|Compound:caspofungin|FD-Score:4.53|P-value:8.25E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:4.20|P-value:2.42E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.79|P-value:8.07E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:5.13|P-value:9.85E-6 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:5.43|P-value:3.12E-6 ID:SGTC_549|Compound:1661-1316|FD-Score:3.87|P-value:6.44E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:3.74|P-value:9.27E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:5.72|P-value:9.60E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:4.69|P-value:4.78E-5 ID:SGTC_631|Compound:k072-0230|FD-Score:5.66|P-value:1.27E-6 ID:SGTC_666|Compound:0272-0154|FD-Score:3.73|P-value:9.53E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.09|P-value:3.35E-4 ID:SGTC_720|Compound:r052-1644|FD-Score:-4.07|P-value:3.36E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:3.81|P-value:7.75E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:4.26|P-value:2.01E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:6.34|P-value:6.64E-8 ID:SGTC_885|Compound:0865-0106|FD-Score:4.74|P-value:4.10E-5 ID:SGTC_895|Compound:0964-0006|FD-Score:6.62|P-value:1.82E-8 ID:SGTC_951|Compound:1171-0579|FD-Score:4.02|P-value:4.17E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:5.71|P-value:1.02E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:4.38|P-value:1.34E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:4.13|P-value:2.97E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:5.60|P-value:1.58E-6 ID:SGTC_1224|Compound:0823-0416|FD-Score:4.10|P-value:3.30E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.91|P-value:5.72E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.86|P-value:6.63E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:6.11|P-value:1.85E-7 ID:SGTC_1474|Compound:0330-0125|FD-Score:3.93|P-value:5.49E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.73|P-value:9.66E-4 ID:SGTC_1740|Compound:st038286|FD-Score:4.01|P-value:4.33E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.75|P-value:9.16E-4 ID:SGTC_1774|Compound:st048371|FD-Score:5.13|P-value:9.97E-6 ID:SGTC_1806|Compound:st050005|FD-Score:4.08|P-value:3.42E-4 ID:SGTC_1815|Compound:st051655|FD-Score:4.52|P-value:8.43E-5 ID:SGTC_1816|Compound:st051957|FD-Score:3.76|P-value:8.91E-4 ID:SGTC_1826|Compound:st055655|FD-Score:7.32|P-value:5.52E-10 ID:SGTC_1845|Compound:st056753|FD-Score:8.19|P-value:4.79E-12 ID:SGTC_2013|Compound:4024628|FD-Score:-4.12|P-value:2.95E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-4.87|P-value:2.39E-5 ID:SGTC_2016|Compound:5121758|FD-Score:5.95|P-value:3.69E-7 ID:SGTC_2023|Compound:5144124|FD-Score:-4.37|P-value:1.31E-4 ID:SGTC_2041|Compound:5107442|FD-Score:5.33|P-value:4.60E-6 ID:SGTC_2135|Compound:5326629|FD-Score:4.13|P-value:3.01E-4 ID:SGTC_2163|Compound:5671233|FD-Score:5.49|P-value:2.48E-6 ID:SGTC_2248|Compound:tofa|FD-Score:-4.09|P-value:3.24E-4 ID:SGTC_2254|Compound:7938975|FD-Score:-4.02|P-value:4.00E-4 ID:SGTC_2261|Compound:9010538|FD-Score:-6.19|P-value:1.22E-7 ID:SGTC_2277|Compound:7947813|FD-Score:4.76|P-value:3.72E-5 ID:SGTC_2311|Compound:7694451|FD-Score:4.89|P-value:2.35E-5 ID:SGTC_2336|Compound:9001819|FD-Score:5.06|P-value:1.29E-5 ID:SGTC_2378|Compound:9074213|FD-Score:7.08|P-value:1.89E-9 ID:SGTC_2381|Compound:9035592|FD-Score:4.52|P-value:8.64E-5 ID:SGTC_2417|Compound:cerulenin|FD-Score:3.82|P-value:7.50E-4 ID:SGTC_2470|Compound:5664817|FD-Score:4.54|P-value:8.08E-5 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:4.36|P-value:1.43E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:4.05|P-value:3.84E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.12|P-value:3.04E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.99|P-value:4.58E-4 ID:SGTC_2714|Compound:st079313|FD-Score:4.67|P-value:5.13E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.85|P-value:2.72E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:5.31|P-value:4.96E-6 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:6.00|P-value:2.98E-7 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-5.59|P-value:1.59E-6 ID:SGTC_2779|Compound:methotrexate|FD-Score:-5.38|P-value:3.59E-6 ID:SGTC_2798|Compound:7813637|FD-Score:3.79|P-value:8.15E-4 ID:SGTC_2819|Compound:7996655|FD-Score:4.59|P-value:6.68E-5 ID:SGTC_2851|Compound:9015922|FD-Score:-3.82|P-value:7.17E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-4.00|P-value:4.20E-4 ID:SGTC_2985|Compound:9022375|FD-Score:7.87|P-value:2.80E-11 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.86|P-value:6.71E-4 ID:SGTC_3064|Compound:1327-0027|FD-Score:5.16|P-value:8.88E-6 ID:SGTC_3126|Compound:9127922|FD-Score:-4.34|P-value:1.45E-4 ID:SGTC_3131|Compound:9127911|FD-Score:-4.11|P-value:3.01E-4 ID:SGTC_3140|Compound:9094243|FD-Score:4.86|P-value:2.66E-5 ID:SGTC_3277|Compound:9138724|FD-Score:4.99|P-value:1.65E-5 ID:SGTC_3337|Compound:9144342|FD-Score:3.92|P-value:5.54E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.88|P-value:6.27E-4

Top fitness defect scores for YIL021W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1845 st056753 8.19 3.0 µM 4.79E-12 TSC3-RPN4 1 1.36E-16 2.87
2 SGTC_2985 9022375 7.87 71.4 µM 2.80E-11 RPP1 & pyrimidine depletion 1 1.70E-15 1.43
3 SGTC_1826 st055655 7.32 41.6 µM 5.52E-10 ERG2 1 1.22E-13 3.15
4 SGTC_2378 9074213 7.08 121.4 µM 1.89E-9 RSC complex & mRNA processing 6 7.12E-13 0.14
5 SGTC_895 0964-0006 6.62 13.7 µM 1.82E-8 2 1.81E-11 1.09
6 SGTC_871 0574-0004 6.34 149.0 µM 6.64E-8 14 1.15E-10 0.01
7 SGTC_274 sirtinol 6.20 40.1 µM 1.25E-7 7 2.83E-10 0.81
8 SGTC_1374 2889-5571 6.11 141.0 µM 1.85E-7 RSC complex & mRNA processing 19 4.99E-10 0.07
9 SGTC_2768 sulfasalazine 6.00 50.2 µM 2.98E-7 2 9.82E-10 0.80
10 SGTC_2016 5121758 5.95 140.0 µM 3.69E-7 redox potentiating 5 1.34E-9 0.08
11 SGTC_586 0957-0265 5.72 8.2 µM 9.60E-7 cell wall 25 5.22E-9 0.04
12 SGTC_994 3910-0338 5.71 138.0 µM 1.02E-6 calcium & mitochondrial duress 2 5.67E-9 0.50
13 SGTC_20 0481-0013 5.67 4.9 µM 1.18E-6 3 7.04E-9 1.21
14 SGTC_631 k072-0230 5.66 41.5 µM 1.27E-6 60S ribosome export 2 7.76E-9 1.74
15 SGTC_1121 fusaric acid 5.60 630.5 nM 1.58E-6 2 1.06E-8 1.68
16 SGTC_2163 5671233 5.49 125.0 µM 2.48E-6 4 2.02E-8 0.09
17 SGTC_546 peri-Naphthalide 5.43 147.0 µM 3.12E-6 DNA damage response 10 2.80E-8 0.02
18 SGTC_2041 5107442 5.33 45.3 µM 4.60E-6 RSC complex & mRNA processing 20 4.86E-8 0.06
19 SGTC_2766 oltipraz 5.31 83.9 µM 4.96E-6 heme requiring 12 5.43E-8 0.55
20 SGTC_3064 1327-0027 5.16 10.0 µM 8.88E-6 3 1.24E-7 0.92

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.268 3.80E-56 YDR404C RPB7 het RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
0.234 6.34E-43 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.213 1.06E-35 YML127W RSC9 het Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
0.211 4.99E-35 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.203 1.85E-32 YPR107C YTH1 het Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
0.188 5.00E-28 YFL002C SPB4 het Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
0.183 9.61E-27 YGL093W SPC105 het Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
0.175 1.63E-24 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.172 1.32E-23 YFR037C RSC8 het Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
0.167 2.60E-22 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.166 5.15E-22 YOL005C RPB11 het RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
0.160 1.01E-20 YBL026W LSM2 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.159 1.82E-20 YAL001C TFC3 het Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
0.159 2.02E-20 YIL126W STH1 het ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
0.156 1.29E-19 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes