YIL023C / YKE4

Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL023C / YKE4

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-4.53|P-value:1.24E-6 ID:SGTC_45|Compound:0kpi-0036|FD-Score:4.05|P-value:8.00E-6 ID:SGTC_60|Compound:0986-0278|FD-Score:-3.15|P-value:5.62E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.03|P-value:5.77E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:3.25|P-value:2.51E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:3.19|P-value:3.16E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.52|P-value:8.34E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.93|P-value:2.35E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:3.28|P-value:2.26E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.40|P-value:1.37E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.79|P-value:2.63E-5 ID:SGTC_557|Compound:1315-0110|FD-Score:3.57|P-value:6.92E-5 ID:SGTC_643|Compound:k048-0106|FD-Score:2.93|P-value:8.41E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.62|P-value:4.38E-7 ID:SGTC_777|Compound:1315-0167|FD-Score:3.55|P-value:7.40E-5 ID:SGTC_843|Compound:0310-0051|FD-Score:-3.33|P-value:2.92E-4 ID:SGTC_855|Compound:0428-0020|FD-Score:6.19|P-value:2.54E-11 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.92|P-value:2.37E-5 ID:SGTC_993|Compound:3909-8757|FD-Score:3.32|P-value:1.92E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.03|P-value:5.76E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.39|P-value:2.25E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:3.18|P-value:3.39E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:-4.37|P-value:2.90E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.28|P-value:2.25E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.03|P-value:5.91E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:2.99|P-value:6.92E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.19|P-value:4.97E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.34|P-value:1.81E-4 ID:SGTC_1604|Compound:st002052|FD-Score:3.26|P-value:2.39E-4 ID:SGTC_1610|Compound:st001799|FD-Score:3.55|P-value:7.31E-5 ID:SGTC_1616|Compound:st002734|FD-Score:4.02|P-value:8.89E-6 ID:SGTC_1655|Compound:st012546|FD-Score:-4.35|P-value:3.17E-6 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.21|P-value:2.93E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.16|P-value:5.53E-4 ID:SGTC_2184|Compound:6075246|FD-Score:4.20|P-value:3.72E-6 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.38|P-value:1.52E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.63|P-value:8.47E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.82|P-value:1.49E-7 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.51|P-value:8.98E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.19|P-value:3.22E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.14|P-value:3.90E-4 ID:SGTC_2873|Compound:9040596|FD-Score:-3.23|P-value:4.22E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.56|P-value:7.10E-5 ID:SGTC_2887|Compound:9044784|FD-Score:-3.51|P-value:1.43E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.01|P-value:9.35E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.52|P-value:8.63E-5 ID:SGTC_3114|Compound:9122766|FD-Score:2.95|P-value:7.99E-4 ID:SGTC_3116|Compound:9124943|FD-Score:2.99|P-value:6.89E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-3.11|P-value:6.55E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.53|P-value:1.24E-6 ID:SGTC_45|Compound:0kpi-0036|FD-Score:4.05|P-value:8.00E-6 ID:SGTC_60|Compound:0986-0278|FD-Score:-3.15|P-value:5.62E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.03|P-value:5.77E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:3.25|P-value:2.51E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:3.19|P-value:3.16E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.52|P-value:8.34E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.93|P-value:2.35E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:3.28|P-value:2.26E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.40|P-value:1.37E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.79|P-value:2.63E-5 ID:SGTC_557|Compound:1315-0110|FD-Score:3.57|P-value:6.92E-5 ID:SGTC_643|Compound:k048-0106|FD-Score:2.93|P-value:8.41E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.62|P-value:4.38E-7 ID:SGTC_777|Compound:1315-0167|FD-Score:3.55|P-value:7.40E-5 ID:SGTC_843|Compound:0310-0051|FD-Score:-3.33|P-value:2.92E-4 ID:SGTC_855|Compound:0428-0020|FD-Score:6.19|P-value:2.54E-11 ID:SGTC_907|Compound:3448-6986|FD-Score:-3.92|P-value:2.37E-5 ID:SGTC_993|Compound:3909-8757|FD-Score:3.32|P-value:1.92E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.03|P-value:5.76E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.39|P-value:2.25E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:3.18|P-value:3.39E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:-4.37|P-value:2.90E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.28|P-value:2.25E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.03|P-value:5.91E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:2.99|P-value:6.92E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.19|P-value:4.97E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.34|P-value:1.81E-4 ID:SGTC_1604|Compound:st002052|FD-Score:3.26|P-value:2.39E-4 ID:SGTC_1610|Compound:st001799|FD-Score:3.55|P-value:7.31E-5 ID:SGTC_1616|Compound:st002734|FD-Score:4.02|P-value:8.89E-6 ID:SGTC_1655|Compound:st012546|FD-Score:-4.35|P-value:3.17E-6 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.21|P-value:2.93E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.16|P-value:5.53E-4 ID:SGTC_2184|Compound:6075246|FD-Score:4.20|P-value:3.72E-6 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.38|P-value:1.52E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.63|P-value:8.47E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.82|P-value:1.49E-7 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.51|P-value:8.98E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.19|P-value:3.22E-4 ID:SGTC_2671|Compound:danthron|FD-Score:3.14|P-value:3.90E-4 ID:SGTC_2873|Compound:9040596|FD-Score:-3.23|P-value:4.22E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.56|P-value:7.10E-5 ID:SGTC_2887|Compound:9044784|FD-Score:-3.51|P-value:1.43E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.01|P-value:9.35E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.52|P-value:8.63E-5 ID:SGTC_3114|Compound:9122766|FD-Score:2.95|P-value:7.99E-4 ID:SGTC_3116|Compound:9124943|FD-Score:2.99|P-value:6.89E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-3.11|P-value:6.55E-4

Top fitness defect scores for YIL023C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_855 0428-0020 6.19 25.0 µM 2.54E-11 Golgi 3 3.02E-10
2 SGTC_2552 juarezic acid 4.82 87.0 µM 1.49E-7 TSC3-RPN4 16 7.30E-7
3 SGTC_701 4424-0219 4.62 185.0 µM 4.38E-7 calcium & mitochondrial duress 14 1.92E-6
4 SGTC_2184 6075246 4.20 200.0 µM 3.72E-6 18 1.31E-5
5 SGTC_45 0kpi-0036 4.05 14.9 µM 8.00E-6 excess fatty acid 21 2.60E-5
6 SGTC_1616 st002734 4.02 56.1 µM 8.89E-6 29 2.86E-5
7 SGTC_493 zm226600 3.79 134.0 µM 2.63E-5 mitochondrial processes 18 7.55E-5
8 SGTC_557 1315-0110 3.57 16.2 µM 6.92E-5 Golgi 30 1.80E-4
9 SGTC_2876 9059903 3.56 26.0 µM 7.10E-5 19 1.84E-4
10 SGTC_1610 st001799 3.55 48.1 µM 7.31E-5 20 1.89E-4
11 SGTC_777 1315-0167 3.55 517.0 µM 7.40E-5 Golgi 40 1.91E-4
12 SGTC_154 3389-1638 3.52 511.1 µM 8.34E-5 37 2.13E-4
13 SGTC_3108 9121785 3.52 49.5 µM 8.63E-5 30 2.19E-4
14 SGTC_2555 cholic acid 3.51 100.0 µM 8.98E-5 28 2.27E-4
15 SGTC_419 nsc-638432 3.40 893.0 µM 1.37E-4 DNA damage response 25 3.31E-4
16 SGTC_2420 aureobasidin a 3.38 50.0 nM 1.52E-4 68 3.65E-4
17 SGTC_1602 st001878 3.34 87.6 µM 1.81E-4 42 4.26E-4
18 SGTC_993 3909-8757 3.32 62.7 µM 1.92E-4 49 4.49E-4
19 SGTC_1506 4226-2149 3.28 212.0 µM 2.25E-4 62 5.17E-4
20 SGTC_360 0957-0399 3.28 1.0 µM 2.26E-4 cell wall 75 5.19E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.106 7.01E-10 YLR310C CDC25 het Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
0.098 1.25E-8 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.091 1.36E-7 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.090 1.69E-7 YJL034W KAR2 het ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
0.086 6.42E-7 YPR048W TAH18 het Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance
0.085 7.25E-7 YAL060W BDH1 hom NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
0.085 7.80E-7 YNL316C PHA2 hom Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway
0.082 2.00E-6 YIL007C NAS2 hom Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress
0.079 4.25E-6 YKL105C SEG2 hom Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes
0.077 8.95E-6 YIL038C NOT3 hom Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
0.076 1.15E-5 YOR014W RTS1 hom B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
0.074 1.56E-5 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.074 1.92E-5 YMR016C SOK2 hom Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
0.072 3.21E-5 YNR008W LRO1 hom Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase
0.070 5.48E-5 YDR229W IVY1 hom Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase