YIL027C / EMC5

Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL027C / EMC5

Click on Significant Values for Screen Details ID:SGTC_90|Compound:0870-0863|FD-Score:3.68|P-value:2.91E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:6.44|P-value:7.08E-10 ID:SGTC_114|Compound:0364-0001|FD-Score:4.57|P-value:9.16E-6 ID:SGTC_152|Compound:4469-0003|FD-Score:3.80|P-value:1.86E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:4.70|P-value:5.24E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:3.98|P-value:9.72E-5 ID:SGTC_562|Compound:3772-2733|FD-Score:4.79|P-value:3.48E-6 ID:SGTC_654|Compound:1124-0271|FD-Score:4.24|P-value:3.49E-5 ID:SGTC_656|Compound:4090-1979|FD-Score:8.00|P-value:2.34E-14 ID:SGTC_658|Compound:3253-0951|FD-Score:5.45|P-value:1.54E-7 ID:SGTC_663|Compound:1486-0733|FD-Score:-3.34|P-value:6.67E-4 ID:SGTC_755|Compound:1995-0008|FD-Score:3.66|P-value:3.07E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.49|P-value:5.52E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:-4.82|P-value:1.95E-6 ID:SGTC_770|Compound:0389-1121|FD-Score:3.61|P-value:3.72E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:4.43|P-value:1.62E-5 ID:SGTC_917|Compound:harmaline|FD-Score:3.98|P-value:9.76E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:7.06|P-value:1.50E-11 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-3.41|P-value:5.28E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-3.98|P-value:6.69E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.40|P-value:5.38E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:4.12|P-value:5.66E-5 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.63|P-value:3.45E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.66|P-value:3.14E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:-4.99|P-value:8.78E-7 ID:SGTC_1554|Compound:st069299|FD-Score:3.77|P-value:2.07E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.62|P-value:3.60E-4 ID:SGTC_1750|Compound:st081859|FD-Score:6.15|P-value:3.57E-9 ID:SGTC_1754|Compound:st042065|FD-Score:3.77|P-value:2.07E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:4.95|P-value:1.66E-6 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.80|P-value:1.85E-4 ID:SGTC_1900|Compound:st060200|FD-Score:4.80|P-value:3.37E-6 ID:SGTC_1936|Compound:st074407|FD-Score:3.44|P-value:6.63E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-3.24|P-value:9.00E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-3.38|P-value:5.74E-4 ID:SGTC_2136|Compound:5328288|FD-Score:-3.66|P-value:2.17E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.39|P-value:1.97E-5 ID:SGTC_2292|Compound:7671323|FD-Score:3.32|P-value:9.73E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.84|P-value:2.05E-8 ID:SGTC_2433|Compound:5607874|FD-Score:4.83|P-value:2.92E-6 ID:SGTC_2435|Compound:5706476|FD-Score:3.38|P-value:7.93E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-3.74|P-value:1.65E-4 ID:SGTC_2481|Compound:5478948|FD-Score:3.54|P-value:4.61E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.41|P-value:7.19E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:5.83|P-value:2.13E-8 ID:SGTC_2645|Compound:abamectin|FD-Score:3.70|P-value:2.73E-4 ID:SGTC_2862|Compound:9027634|FD-Score:4.74|P-value:4.41E-6 ID:SGTC_2948|Compound:9070189|FD-Score:4.85|P-value:2.63E-6 ID:SGTC_3132|Compound:9126706|FD-Score:3.43|P-value:6.71E-4 ID:SGTC_3174|Compound:9107204|FD-Score:3.41|P-value:7.32E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:3.68|P-value:2.91E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:6.44|P-value:7.08E-10 ID:SGTC_114|Compound:0364-0001|FD-Score:4.57|P-value:9.16E-6 ID:SGTC_152|Compound:4469-0003|FD-Score:3.80|P-value:1.86E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:4.70|P-value:5.24E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:3.98|P-value:9.72E-5 ID:SGTC_562|Compound:3772-2733|FD-Score:4.79|P-value:3.48E-6 ID:SGTC_654|Compound:1124-0271|FD-Score:4.24|P-value:3.49E-5 ID:SGTC_656|Compound:4090-1979|FD-Score:8.00|P-value:2.34E-14 ID:SGTC_658|Compound:3253-0951|FD-Score:5.45|P-value:1.54E-7 ID:SGTC_663|Compound:1486-0733|FD-Score:-3.34|P-value:6.67E-4 ID:SGTC_755|Compound:1995-0008|FD-Score:3.66|P-value:3.07E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.49|P-value:5.52E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:-4.82|P-value:1.95E-6 ID:SGTC_770|Compound:0389-1121|FD-Score:3.61|P-value:3.72E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:4.43|P-value:1.62E-5 ID:SGTC_917|Compound:harmaline|FD-Score:3.98|P-value:9.76E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:7.06|P-value:1.50E-11 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-3.41|P-value:5.28E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:-3.98|P-value:6.69E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:-3.40|P-value:5.38E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:4.12|P-value:5.66E-5 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.63|P-value:3.45E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.66|P-value:3.14E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:-4.99|P-value:8.78E-7 ID:SGTC_1554|Compound:st069299|FD-Score:3.77|P-value:2.07E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.62|P-value:3.60E-4 ID:SGTC_1750|Compound:st081859|FD-Score:6.15|P-value:3.57E-9 ID:SGTC_1754|Compound:st042065|FD-Score:3.77|P-value:2.07E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:4.95|P-value:1.66E-6 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.80|P-value:1.85E-4 ID:SGTC_1900|Compound:st060200|FD-Score:4.80|P-value:3.37E-6 ID:SGTC_1936|Compound:st074407|FD-Score:3.44|P-value:6.63E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-3.24|P-value:9.00E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-3.38|P-value:5.74E-4 ID:SGTC_2136|Compound:5328288|FD-Score:-3.66|P-value:2.17E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.39|P-value:1.97E-5 ID:SGTC_2292|Compound:7671323|FD-Score:3.32|P-value:9.73E-4 ID:SGTC_2296|Compound:7971656|FD-Score:5.84|P-value:2.05E-8 ID:SGTC_2433|Compound:5607874|FD-Score:4.83|P-value:2.92E-6 ID:SGTC_2435|Compound:5706476|FD-Score:3.38|P-value:7.93E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-3.74|P-value:1.65E-4 ID:SGTC_2481|Compound:5478948|FD-Score:3.54|P-value:4.61E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.41|P-value:7.19E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:5.83|P-value:2.13E-8 ID:SGTC_2645|Compound:abamectin|FD-Score:3.70|P-value:2.73E-4 ID:SGTC_2862|Compound:9027634|FD-Score:4.74|P-value:4.41E-6 ID:SGTC_2948|Compound:9070189|FD-Score:4.85|P-value:2.63E-6 ID:SGTC_3132|Compound:9126706|FD-Score:3.43|P-value:6.71E-4 ID:SGTC_3174|Compound:9107204|FD-Score:3.41|P-value:7.32E-4

Top fitness defect scores for YIL027C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_656 4090-1979 8.00 187.0 µM 2.34E-14 1 6.05E-16
2 SGTC_984 1492-0255 7.06 2.9 µM 1.50E-11 SWF1 & branched chain AA biosynthesis 6 8.31E-13
3 SGTC_103 0844-0104 6.44 51.8 µM 7.08E-10 ERAD & cell cycle 68 6.17E-11
4 SGTC_1750 st081859 6.15 2.4 µM 3.57E-9 endomembrane recycling 104 3.77E-10
5 SGTC_2296 7971656 5.84 126.5 µM 2.05E-8 14 2.66E-9
6 SGTC_2626 ethyl orsellinate 5.83 100.0 µM 2.13E-8 6 2.78E-9
7 SGTC_658 3253-0951 5.45 102.0 µM 1.54E-7 calcium & mitochondrial duress 22 2.54E-8
8 SGTC_1872 amphotericin b 4.95 148.0 nM 1.66E-6 RNA pol III & RNase P/MRP 17 3.62E-7
9 SGTC_2948 9070189 4.85 9.4 µM 2.63E-6 plasma membrane duress 33 6.07E-7
10 SGTC_2433 5607874 4.83 27.8 µM 2.92E-6 6 6.84E-7
11 SGTC_1900 st060200 4.80 72.8 µM 3.37E-6 10 8.02E-7
12 SGTC_562 3772-2733 4.79 112.0 µM 3.48E-6 ERG2 55 8.31E-7
13 SGTC_2862 9027634 4.74 71.4 µM 4.41E-6 ERG2 18 1.08E-6
14 SGTC_176 1486-1328 4.70 72.8 µM 5.24E-6 38 1.31E-6
15 SGTC_114 0364-0001 4.57 490.3 µM 9.16E-6 28 2.46E-6
16 SGTC_913 3487-1245 4.43 167.0 µM 1.62E-5 17 4.64E-6
17 SGTC_2249 haloperidol 4.39 116.4 µM 1.97E-5 ergosterol depletion effects on membrane 88 5.78E-6
18 SGTC_654 1124-0271 4.24 80.9 µM 3.49E-5 19 1.09E-5
19 SGTC_1266 0828-0227 4.12 32.9 µM 5.66E-5 19 1.88E-5
20 SGTC_352 k048-0007 3.98 5.1 µM 9.72E-5 ERAD & cell cycle 79 3.45E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.209 2.04E-34 YJR088C EMC2 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.198 6.50E-31 YKL207W EMC3 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111
0.148 5.29E-18 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.140 3.24E-16 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.132 1.52E-14 YLL014W EMC6 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93
0.131 3.28E-14 YMR007W_d YMR007W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.129 6.81E-14 YHR104W GRE3 hom Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
0.127 1.83E-13 YMR052C-A_d YMR052C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.126 2.71E-13 YPL176C TRE1 hom Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication
0.123 8.70E-13 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.121 1.77E-12 YDL122W UBP1 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
0.119 4.11E-12 YJR087W_d YJR087W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
0.115 2.92E-11 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.112 6.87E-11 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.112 7.55E-11 YPR153W YPR153W hom Putative protein of unknown function