YIL030C / SSM4

Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL030C / SSM4

Click on Significant Values for Screen Details ID:SGTC_34|Compound:0469-0683|FD-Score:3.20|P-value:7.32E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.72|P-value:8.07E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:8.81|P-value:6.52E-19 ID:SGTC_103|Compound:0844-0104|FD-Score:14.40|P-value:2.21E-47 ID:SGTC_137|Compound:0438-0332|FD-Score:3.90|P-value:5.23E-5 ID:SGTC_228|Compound:caffeine|FD-Score:4.55|P-value:2.99E-6 ID:SGTC_299|Compound:1273-0059|FD-Score:3.16|P-value:8.55E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:15.60|P-value:4.98E-55 ID:SGTC_373|Compound:0844-0104|FD-Score:30.20|P-value:1.04E-201 ID:SGTC_384|Compound:k007-0144|FD-Score:28.30|P-value:1.32E-177 ID:SGTC_385|Compound:1494-0393|FD-Score:3.52|P-value:2.31E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:4.05|P-value:2.82E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:8.24|P-value:8.66E-17 ID:SGTC_568|Compound:1193-0097|FD-Score:14.00|P-value:4.69E-45 ID:SGTC_637|Compound:1181-0519|FD-Score:16.30|P-value:6.02E-60 ID:SGTC_733|Compound:4069-0007|FD-Score:35.40|P-value:5.80E-276 ID:SGTC_805|Compound:3852-0218|FD-Score:3.97|P-value:3.91E-5 ID:SGTC_917|Compound:harmaline|FD-Score:3.96|P-value:4.16E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:14.70|P-value:3.41E-49 ID:SGTC_1120|Compound:ficusin|FD-Score:3.58|P-value:1.90E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.94|P-value:4.48E-5 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.35|P-value:4.43E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:27.50|P-value:1.12E-167 ID:SGTC_1187|Compound:1391-0724|FD-Score:4.35|P-value:7.49E-6 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.27|P-value:5.89E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:4.39|P-value:6.18E-6 ID:SGTC_1414|Compound:3917-0015|FD-Score:3.82|P-value:7.38E-5 ID:SGTC_1563|Compound:conessine|FD-Score:3.23|P-value:6.59E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:4.59|P-value:2.42E-6 ID:SGTC_1684|Compound:st024538|FD-Score:3.38|P-value:4.00E-4 ID:SGTC_1852|Compound:st056254|FD-Score:3.82|P-value:7.30E-5 ID:SGTC_2018|Compound:5133068|FD-Score:6.16|P-value:3.83E-10 ID:SGTC_2322|Compound:7413319|FD-Score:6.41|P-value:7.63E-11 ID:SGTC_2343|Compound:7990716|FD-Score:6.78|P-value:6.41E-12 ID:SGTC_2349|Compound:9023730|FD-Score:3.84|P-value:6.81E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.30|P-value:5.17E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:-3.24|P-value:5.11E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:3.26|P-value:6.09E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.15|P-value:8.76E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-3.25|P-value:4.83E-4 ID:SGTC_2818|Compound:7996756|FD-Score:3.31|P-value:4.98E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.46|P-value:2.88E-4 ID:SGTC_2931|Compound:9014674|FD-Score:4.02|P-value:3.15E-5 ID:SGTC_2937|Compound:9044753|FD-Score:3.24|P-value:6.51E-4 ID:SGTC_3053|Compound:9095903|FD-Score:3.34|P-value:4.51E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:-3.28|P-value:4.33E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.46|P-value:2.29E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.20|P-value:7.33E-4 ID:SGTC_3302|Compound:9125395|FD-Score:3.66|P-value:1.37E-4 ID:SGTC_3344|Compound:9148862|FD-Score:3.97|P-value:3.87E-5 ID:SGTC_34|Compound:0469-0683|FD-Score:3.20|P-value:7.32E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.72|P-value:8.07E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:8.81|P-value:6.52E-19 ID:SGTC_103|Compound:0844-0104|FD-Score:14.40|P-value:2.21E-47 ID:SGTC_137|Compound:0438-0332|FD-Score:3.90|P-value:5.23E-5 ID:SGTC_228|Compound:caffeine|FD-Score:4.55|P-value:2.99E-6 ID:SGTC_299|Compound:1273-0059|FD-Score:3.16|P-value:8.55E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:15.60|P-value:4.98E-55 ID:SGTC_373|Compound:0844-0104|FD-Score:30.20|P-value:1.04E-201 ID:SGTC_384|Compound:k007-0144|FD-Score:28.30|P-value:1.32E-177 ID:SGTC_385|Compound:1494-0393|FD-Score:3.52|P-value:2.31E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:4.05|P-value:2.82E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:8.24|P-value:8.66E-17 ID:SGTC_568|Compound:1193-0097|FD-Score:14.00|P-value:4.69E-45 ID:SGTC_637|Compound:1181-0519|FD-Score:16.30|P-value:6.02E-60 ID:SGTC_733|Compound:4069-0007|FD-Score:35.40|P-value:5.80E-276 ID:SGTC_805|Compound:3852-0218|FD-Score:3.97|P-value:3.91E-5 ID:SGTC_917|Compound:harmaline|FD-Score:3.96|P-value:4.16E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:14.70|P-value:3.41E-49 ID:SGTC_1120|Compound:ficusin|FD-Score:3.58|P-value:1.90E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.94|P-value:4.48E-5 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.35|P-value:4.43E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:27.50|P-value:1.12E-167 ID:SGTC_1187|Compound:1391-0724|FD-Score:4.35|P-value:7.49E-6 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.27|P-value:5.89E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:4.39|P-value:6.18E-6 ID:SGTC_1414|Compound:3917-0015|FD-Score:3.82|P-value:7.38E-5 ID:SGTC_1563|Compound:conessine|FD-Score:3.23|P-value:6.59E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:4.59|P-value:2.42E-6 ID:SGTC_1684|Compound:st024538|FD-Score:3.38|P-value:4.00E-4 ID:SGTC_1852|Compound:st056254|FD-Score:3.82|P-value:7.30E-5 ID:SGTC_2018|Compound:5133068|FD-Score:6.16|P-value:3.83E-10 ID:SGTC_2322|Compound:7413319|FD-Score:6.41|P-value:7.63E-11 ID:SGTC_2343|Compound:7990716|FD-Score:6.78|P-value:6.41E-12 ID:SGTC_2349|Compound:9023730|FD-Score:3.84|P-value:6.81E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.30|P-value:5.17E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:-3.24|P-value:5.11E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:3.26|P-value:6.09E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.15|P-value:8.76E-4 ID:SGTC_2807|Compound:7945238|FD-Score:-3.25|P-value:4.83E-4 ID:SGTC_2818|Compound:7996756|FD-Score:3.31|P-value:4.98E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.46|P-value:2.88E-4 ID:SGTC_2931|Compound:9014674|FD-Score:4.02|P-value:3.15E-5 ID:SGTC_2937|Compound:9044753|FD-Score:3.24|P-value:6.51E-4 ID:SGTC_3053|Compound:9095903|FD-Score:3.34|P-value:4.51E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:-3.28|P-value:4.33E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.46|P-value:2.29E-4 ID:SGTC_3296|Compound:9122258|FD-Score:3.20|P-value:7.33E-4 ID:SGTC_3302|Compound:9125395|FD-Score:3.66|P-value:1.37E-4 ID:SGTC_3344|Compound:9148862|FD-Score:3.97|P-value:3.87E-5

Top fitness defect scores for YIL030C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_733 4069-0007 35.40 56.1 µM 5.80E-276 ERAD & cell cycle 1 1.55E-274
2 SGTC_373 0844-0104 30.20 25.9 µM 1.04E-201 ERAD & cell cycle 1 9.79E-201
3 SGTC_384 k007-0144 28.30 5.2 µM 1.32E-177 ERAD & cell cycle 1 8.87E-177
4 SGTC_1161 k007-0610 27.50 110.0 µM 1.12E-167 ERAD & cell cycle 1 6.58E-167
5 SGTC_637 1181-0519 16.30 7.3 µM 6.02E-60 fatty acid desaturase (OLE1) 7 8.89E-60
6 SGTC_352 k048-0007 15.60 5.1 µM 4.98E-55 ERAD & cell cycle 3 6.95E-55
7 SGTC_1026 k007-1105 14.70 30.6 µM 3.41E-49 ERAD & cell cycle 5 4.45E-49
8 SGTC_103 0844-0104 14.40 51.8 µM 2.21E-47 ERAD & cell cycle 10 2.83E-47
9 SGTC_568 1193-0097 14.00 7.5 µM 4.69E-45 ERAD & cell cycle 5 5.86E-45
10 SGTC_64 000l-0018 8.81 272.1 µM 6.52E-19 ubiquinone biosynthesis & proteasome 42 6.35E-19
11 SGTC_426 0988-0037 8.24 14.6 µM 8.66E-17 ERAD & cell cycle 5 8.31E-17
12 SGTC_2343 7990716 6.78 14.8 µM 6.41E-12 1 5.98E-12
13 SGTC_2322 7413319 6.41 38.2 µM 7.63E-11 ERAD & cell cycle 57 7.07E-11
14 SGTC_2018 5133068 6.16 143.0 µM 3.83E-10 3 3.54E-10
15 SGTC_1569 carminic acid 4.59 40.6 µM 2.42E-6 8 2.22E-6
16 SGTC_228 caffeine 4.55 993.1 µM 2.99E-6 7 2.74E-6
17 SGTC_1354 1498-1681 4.39 81.0 µM 6.18E-6 10 5.67E-6
18 SGTC_1187 1391-0724 4.35 117.0 µM 7.49E-6 amide catabolism 14 6.87E-6
19 SGTC_400 oxethazaine 4.05 37.5 µM 2.82E-5 calcium & mitochondrial duress 21 2.59E-5
20 SGTC_2931 9014674 4.02 55.7 µM 3.15E-5 19 2.89E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.676 0 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.659 0 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.628 0 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.559 1.12E-274 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.538 5.54E-251 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.475 1.41E-188 YMR052C-A_d YMR052C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.472 7.92E-186 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.462 1.07E-176 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.428 1.08E-149 YKR014C YPT52 hom Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress
0.419 9.50E-143 YMR052W FAR3 hom Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.404 9.94E-132 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.403 5.34E-131 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.388 2.67E-121 YNL287W SEC21 het Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
0.383 1.69E-117 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.374 3.75E-112 YDR200C VPS64 hom Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance