YIL044C / AGE2

ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL044C / AGE2

Click on Significant Values for Screen Details ID:SGTC_124|Compound:0136-0217|FD-Score:3.20|P-value:7.91E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.33|P-value:9.34E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:-3.55|P-value:2.24E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:3.62|P-value:1.76E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:5.81|P-value:4.78E-9 ID:SGTC_181|Compound:1447-1644|FD-Score:3.34|P-value:4.86E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:3.34|P-value:4.89E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.72|P-value:1.20E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.05|P-value:3.17E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:3.37|P-value:4.41E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.35|P-value:8.56E-6 ID:SGTC_644|Compound:1498-1197|FD-Score:4.41|P-value:6.72E-6 ID:SGTC_788|Compound:1182-0090|FD-Score:3.67|P-value:1.47E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:7.13|P-value:9.53E-13 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.85|P-value:7.07E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.30|P-value:5.49E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:-3.97|P-value:4.39E-5 ID:SGTC_1600|Compound:coumarin 106|FD-Score:4.59|P-value:2.87E-6 ID:SGTC_1769|Compound:st044406|FD-Score:-3.14|P-value:9.60E-4 ID:SGTC_1908|Compound:st060353|FD-Score:8.59|P-value:1.03E-17 ID:SGTC_1913|Compound:st060213|FD-Score:3.18|P-value:8.46E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.40|P-value:3.94E-4 ID:SGTC_1919|Compound:st067113|FD-Score:6.34|P-value:1.93E-10 ID:SGTC_1940|Compound:st075990|FD-Score:-3.65|P-value:1.53E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-3.59|P-value:1.91E-4 ID:SGTC_1954|Compound:st077032|FD-Score:3.84|P-value:7.48E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.26|P-value:6.33E-4 ID:SGTC_2166|Compound:5933061|FD-Score:6.72|P-value:1.55E-11 ID:SGTC_2270|Compound:7924575|FD-Score:3.64|P-value:1.63E-4 ID:SGTC_2368|Compound:9071271|FD-Score:4.66|P-value:2.08E-6 ID:SGTC_2479|Compound:5617353|FD-Score:-4.02|P-value:3.54E-5 ID:SGTC_2482|Compound:5981709|FD-Score:4.54|P-value:3.68E-6 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:5.52|P-value:2.47E-8 ID:SGTC_2687|Compound:chenodiol|FD-Score:-4.77|P-value:1.19E-6 ID:SGTC_2786|Compound:7743617|FD-Score:4.51|P-value:4.19E-6 ID:SGTC_2891|Compound:9063876|FD-Score:3.60|P-value:1.89E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.70|P-value:1.27E-4 ID:SGTC_3010|Compound:9080089|FD-Score:3.51|P-value:2.68E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.26|P-value:1.27E-5 ID:SGTC_3252|Compound:9135871|FD-Score:3.45|P-value:3.33E-4 ID:SGTC_3318|Compound:9139014|FD-Score:-3.62|P-value:1.74E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.20|P-value:7.91E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.33|P-value:9.34E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:-3.55|P-value:2.24E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:3.62|P-value:1.76E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:5.81|P-value:4.78E-9 ID:SGTC_181|Compound:1447-1644|FD-Score:3.34|P-value:4.86E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:3.34|P-value:4.89E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.72|P-value:1.20E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.05|P-value:3.17E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:3.37|P-value:4.41E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.35|P-value:8.56E-6 ID:SGTC_644|Compound:1498-1197|FD-Score:4.41|P-value:6.72E-6 ID:SGTC_788|Compound:1182-0090|FD-Score:3.67|P-value:1.47E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:7.13|P-value:9.53E-13 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.85|P-value:7.07E-5 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.30|P-value:5.49E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:-3.97|P-value:4.39E-5 ID:SGTC_1600|Compound:coumarin 106|FD-Score:4.59|P-value:2.87E-6 ID:SGTC_1769|Compound:st044406|FD-Score:-3.14|P-value:9.60E-4 ID:SGTC_1908|Compound:st060353|FD-Score:8.59|P-value:1.03E-17 ID:SGTC_1913|Compound:st060213|FD-Score:3.18|P-value:8.46E-4 ID:SGTC_1916|Compound:st067606|FD-Score:3.40|P-value:3.94E-4 ID:SGTC_1919|Compound:st067113|FD-Score:6.34|P-value:1.93E-10 ID:SGTC_1940|Compound:st075990|FD-Score:-3.65|P-value:1.53E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-3.59|P-value:1.91E-4 ID:SGTC_1954|Compound:st077032|FD-Score:3.84|P-value:7.48E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.26|P-value:6.33E-4 ID:SGTC_2166|Compound:5933061|FD-Score:6.72|P-value:1.55E-11 ID:SGTC_2270|Compound:7924575|FD-Score:3.64|P-value:1.63E-4 ID:SGTC_2368|Compound:9071271|FD-Score:4.66|P-value:2.08E-6 ID:SGTC_2479|Compound:5617353|FD-Score:-4.02|P-value:3.54E-5 ID:SGTC_2482|Compound:5981709|FD-Score:4.54|P-value:3.68E-6 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:5.52|P-value:2.47E-8 ID:SGTC_2687|Compound:chenodiol|FD-Score:-4.77|P-value:1.19E-6 ID:SGTC_2786|Compound:7743617|FD-Score:4.51|P-value:4.19E-6 ID:SGTC_2891|Compound:9063876|FD-Score:3.60|P-value:1.89E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.70|P-value:1.27E-4 ID:SGTC_3010|Compound:9080089|FD-Score:3.51|P-value:2.68E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.26|P-value:1.27E-5 ID:SGTC_3252|Compound:9135871|FD-Score:3.45|P-value:3.33E-4 ID:SGTC_3318|Compound:9139014|FD-Score:-3.62|P-value:1.74E-4

Top fitness defect scores for YIL044C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1908 st060353 8.59 56.5 µM 1.03E-17 RPP1 & pyrimidine depletion 1 4.23E-18
2 SGTC_1063 disulfiram 7.13 1.8 µM 9.53E-13 1 5.13E-13
3 SGTC_2166 5933061 6.72 31.5 µM 1.55E-11 tubulin folding & SWR complex 10 8.89E-12
4 SGTC_1919 st067113 6.34 52.3 µM 1.93E-10 TSC3-RPN4 9 1.18E-10
5 SGTC_178 4092-0806 5.81 87.5 µM 4.78E-9 ergosterol depletion effects on membrane 43 3.15E-9
6 SGTC_2597 laccaic acid a 5.52 100.0 µM 2.47E-8 11 1.69E-8
7 SGTC_2368 9071271 4.66 200.0 µM 2.08E-6 14 1.58E-6
8 SGTC_1600 coumarin 106 4.59 71.1 µM 2.87E-6 15 2.20E-6
9 SGTC_2482 5981709 4.54 142.7 µM 3.68E-6 PDR1 29 2.84E-6
10 SGTC_2786 7743617 4.51 45.5 µM 4.19E-6 16 3.24E-6
11 SGTC_644 1498-1197 4.41 63.3 µM 6.72E-6 8 5.25E-6
12 SGTC_568 1193-0097 4.35 7.5 µM 8.56E-6 ERAD & cell cycle 123 6.73E-6
13 SGTC_138 0850-0259 4.33 40.9 µM 9.34E-6 cell wall 174 7.36E-6
14 SGTC_3068 9116768 4.26 49.5 µM 1.27E-5 13 1.01E-5
15 SGTC_467 ro 31-8220 4.05 72.8 µM 3.17E-5 calcium & mitochondrial duress 72 2.57E-5
16 SGTC_1155 4088-0394 3.85 48.2 µM 7.07E-5 30 5.84E-5
17 SGTC_1954 st077032 3.84 68.9 µM 7.48E-5 24 6.19E-5
18 SGTC_254 nsc-64875 3.72 3.8 µM 1.20E-4 ergosterol depletion effects on membrane 33 1.01E-4
19 SGTC_2894 9048625 3.70 32.5 µM 1.27E-4 17 1.06E-4
20 SGTC_788 1182-0090 3.67 188.0 µM 1.47E-4 endomembrane recycling 88 1.23E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 4.12E-18 YOR045W TOM6 hom Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
0.143 7.97E-17 YJL028W YJL028W hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments
0.116 1.77E-11 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.108 3.67E-10 YDR488C PAC11 hom Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8
0.107 6.10E-10 YJR060W CBF1 hom Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
0.105 1.12E-9 YGR273C_p YGR273C_p hom Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene
0.100 7.66E-9 YNL057W_d YNL057W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.095 3.78E-8 YDR438W THI74 hom Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication
0.095 4.04E-8 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.094 5.46E-8 YKL190W CNB1 hom Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress
0.092 9.40E-8 YPL096W PNG1 hom Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p
0.092 1.02E-7 YMR273C ZDS1 hom Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
0.091 1.47E-7 YJR111C_p YJR111C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.089 2.10E-7 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.089 2.42E-7 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene