YIL075C / RPN2

Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YIL075C / RPN2

Click on Significant Values for Screen Details ID:SGTC_19|Compound:0279-0214|FD-Score:3.15|P-value:6.87E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:4.09|P-value:1.84E-5 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.18|P-value:6.16E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.26|P-value:4.69E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:4.27|P-value:8.68E-6 ID:SGTC_177|Compound:1327-0068|FD-Score:4.07|P-value:2.04E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:3.86|P-value:4.83E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:3.10|P-value:8.16E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.61|P-value:1.30E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.44|P-value:5.36E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.30|P-value:4.10E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.08|P-value:5.46E-7 ID:SGTC_584|Compound:0986-0246|FD-Score:4.20|P-value:1.18E-5 ID:SGTC_674|Compound:0498-0004|FD-Score:5.26|P-value:6.58E-8 ID:SGTC_798|Compound:1287-0182|FD-Score:3.80|P-value:6.07E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:4.10|P-value:1.82E-5 ID:SGTC_957|Compound:1187-1561|FD-Score:3.41|P-value:2.73E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:3.23|P-value:5.23E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:3.10|P-value:8.08E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.48|P-value:2.14E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.93|P-value:3.59E-5 ID:SGTC_1187|Compound:1391-0724|FD-Score:4.16|P-value:1.37E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:-4.03|P-value:6.01E-5 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.64|P-value:2.63E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.40|P-value:2.85E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.66|P-value:1.06E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.16|P-value:6.66E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:-3.33|P-value:7.81E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:6.15|P-value:3.86E-10 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.09|P-value:8.46E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:4.26|P-value:8.87E-6 ID:SGTC_1769|Compound:st044406|FD-Score:3.12|P-value:7.67E-4 ID:SGTC_1797|Compound:st048695|FD-Score:4.17|P-value:1.33E-5 ID:SGTC_1966|Compound:st070145|FD-Score:3.96|P-value:3.24E-5 ID:SGTC_2004|Compound:4023728|FD-Score:3.25|P-value:4.89E-4 ID:SGTC_2112|Compound:5488546|FD-Score:4.07|P-value:2.07E-5 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.13|P-value:1.56E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.74|P-value:7.83E-5 ID:SGTC_2225|Compound:6654152|FD-Score:3.14|P-value:7.03E-4 ID:SGTC_2256|Compound:7954252|FD-Score:3.45|P-value:2.33E-4 ID:SGTC_2301|Compound:7769476|FD-Score:4.39|P-value:4.93E-6 ID:SGTC_2336|Compound:9001819|FD-Score:5.08|P-value:1.69E-7 ID:SGTC_2344|Compound:7990960|FD-Score:3.65|P-value:1.13E-4 ID:SGTC_2400|Compound:5203494|FD-Score:4.05|P-value:2.21E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.63|P-value:1.18E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:5.65|P-value:7.66E-9 ID:SGTC_2517|Compound:totarol|FD-Score:4.09|P-value:1.87E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:5.12|P-value:1.40E-7 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.51|P-value:4.25E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:5.00|P-value:2.66E-7 ID:SGTC_2576|Compound:guaiol|FD-Score:3.38|P-value:3.00E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.65|P-value:1.11E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.76|P-value:7.10E-5 ID:SGTC_2711|Compound:st079103|FD-Score:3.12|P-value:7.47E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.15|P-value:6.93E-4 ID:SGTC_3028|Compound:9087778|FD-Score:3.98|P-value:2.95E-5 ID:SGTC_3075|Compound:9116269|FD-Score:4.12|P-value:1.64E-5 ID:SGTC_3101|Compound:9120531|FD-Score:-3.56|P-value:3.50E-4 ID:SGTC_3110|Compound:9122132|FD-Score:-3.50|P-value:4.45E-4 ID:SGTC_3113|Compound:9123498|FD-Score:3.05|P-value:9.72E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.98|P-value:3.01E-5 ID:SGTC_3266|Compound:9136860|FD-Score:3.23|P-value:5.23E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.08|P-value:8.75E-4 ID:SGTC_19|Compound:0279-0214|FD-Score:3.15|P-value:6.87E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:4.09|P-value:1.84E-5 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.18|P-value:6.16E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.26|P-value:4.69E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:4.27|P-value:8.68E-6 ID:SGTC_177|Compound:1327-0068|FD-Score:4.07|P-value:2.04E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:3.86|P-value:4.83E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:3.10|P-value:8.16E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.61|P-value:1.30E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.44|P-value:5.36E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.30|P-value:4.10E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.08|P-value:5.46E-7 ID:SGTC_584|Compound:0986-0246|FD-Score:4.20|P-value:1.18E-5 ID:SGTC_674|Compound:0498-0004|FD-Score:5.26|P-value:6.58E-8 ID:SGTC_798|Compound:1287-0182|FD-Score:3.80|P-value:6.07E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:4.10|P-value:1.82E-5 ID:SGTC_957|Compound:1187-1561|FD-Score:3.41|P-value:2.73E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:3.23|P-value:5.23E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:3.10|P-value:8.08E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.48|P-value:2.14E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.93|P-value:3.59E-5 ID:SGTC_1187|Compound:1391-0724|FD-Score:4.16|P-value:1.37E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:-4.03|P-value:6.01E-5 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.64|P-value:2.63E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.40|P-value:2.85E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.66|P-value:1.06E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.16|P-value:6.66E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:-3.33|P-value:7.81E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:6.15|P-value:3.86E-10 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.09|P-value:8.46E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:4.26|P-value:8.87E-6 ID:SGTC_1769|Compound:st044406|FD-Score:3.12|P-value:7.67E-4 ID:SGTC_1797|Compound:st048695|FD-Score:4.17|P-value:1.33E-5 ID:SGTC_1966|Compound:st070145|FD-Score:3.96|P-value:3.24E-5 ID:SGTC_2004|Compound:4023728|FD-Score:3.25|P-value:4.89E-4 ID:SGTC_2112|Compound:5488546|FD-Score:4.07|P-value:2.07E-5 ID:SGTC_2129|Compound:girgensonine|FD-Score:4.13|P-value:1.56E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.74|P-value:7.83E-5 ID:SGTC_2225|Compound:6654152|FD-Score:3.14|P-value:7.03E-4 ID:SGTC_2256|Compound:7954252|FD-Score:3.45|P-value:2.33E-4 ID:SGTC_2301|Compound:7769476|FD-Score:4.39|P-value:4.93E-6 ID:SGTC_2336|Compound:9001819|FD-Score:5.08|P-value:1.69E-7 ID:SGTC_2344|Compound:7990960|FD-Score:3.65|P-value:1.13E-4 ID:SGTC_2400|Compound:5203494|FD-Score:4.05|P-value:2.21E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.63|P-value:1.18E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:5.65|P-value:7.66E-9 ID:SGTC_2517|Compound:totarol|FD-Score:4.09|P-value:1.87E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:5.12|P-value:1.40E-7 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.51|P-value:4.25E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:5.00|P-value:2.66E-7 ID:SGTC_2576|Compound:guaiol|FD-Score:3.38|P-value:3.00E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.65|P-value:1.11E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.76|P-value:7.10E-5 ID:SGTC_2711|Compound:st079103|FD-Score:3.12|P-value:7.47E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.15|P-value:6.93E-4 ID:SGTC_3028|Compound:9087778|FD-Score:3.98|P-value:2.95E-5 ID:SGTC_3075|Compound:9116269|FD-Score:4.12|P-value:1.64E-5 ID:SGTC_3101|Compound:9120531|FD-Score:-3.56|P-value:3.50E-4 ID:SGTC_3110|Compound:9122132|FD-Score:-3.50|P-value:4.45E-4 ID:SGTC_3113|Compound:9123498|FD-Score:3.05|P-value:9.72E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.98|P-value:3.01E-5 ID:SGTC_3266|Compound:9136860|FD-Score:3.23|P-value:5.23E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.08|P-value:8.75E-4

Top fitness defect scores for YIL075C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1435 4092-0789 6.15 56.7 µM 3.86E-10 plasma membrane duress 2 3.77E-10 1.69
2 SGTC_2516 gossypol 5.65 71.5 µM 7.66E-9 4 7.91E-9 0.45
3 SGTC_674 0498-0004 5.26 451.0 µM 6.58E-8 heme requiring 7 7.04E-8 0.16
4 SGTC_2519 cryptotanshinone 5.12 88.0 µM 1.40E-7 heme biosynthesis & mitochondrial translocase 10 1.51E-7 0.31
5 SGTC_2336 9001819 5.08 198.7 µM 1.69E-7 heme biosynthesis & mitochondrial translocase 25 1.84E-7 0.00
6 SGTC_2575 plumbagin 5.00 510.0 nM 2.66E-7 superoxide 17 2.91E-7 0.12
7 SGTC_2301 7769476 4.39 200.0 µM 4.93E-6 10 5.62E-6 0.05
8 SGTC_123 0083-0120 4.27 66.7 µM 8.68E-6 10 9.96E-6 0.22
9 SGTC_1631 benzylvanillin 4.26 81.9 µM 8.87E-6 Golgi 5 1.02E-5 0.27
10 SGTC_584 0986-0246 4.20 15.4 µM 1.18E-5 heme requiring 23 1.36E-5 0.09
11 SGTC_1797 st048695 4.17 56.8 µM 1.33E-5 NEO1 13 1.53E-5 0.04
12 SGTC_1187 1391-0724 4.16 117.0 µM 1.37E-5 amide catabolism 3 1.58E-5 0.42
13 SGTC_2129 girgensonine 4.13 121.0 µM 1.56E-5 heme biosynthesis & mitochondrial translocase 21 1.80E-5 0.01
14 SGTC_3075 9116269 4.12 49.5 µM 1.64E-5 6 1.89E-5 0.27
15 SGTC_862 0452-0056 4.10 10.2 µM 1.82E-5 azole & statin 3 2.10E-5 0.78
16 SGTC_32 0928-0142 4.09 34.1 µM 1.84E-5 TSC3-RPN4 7 2.13E-5 0.61
17 SGTC_2517 totarol 4.09 10.0 µM 1.87E-5 4 2.17E-5 0.08
18 SGTC_177 1327-0068 4.07 8.4 µM 2.04E-5 21 2.36E-5 0.02
19 SGTC_2112 5488546 4.07 200.0 µM 2.07E-5 TSC3-RPN4 6 2.40E-5 0.05
20 SGTC_2400 5203494 4.05 178.7 µM 2.21E-5 RSC complex & mRNA processing 18 2.56E-5 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.225 7.56E-40 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.204 6.83E-33 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.204 7.98E-33 YDR427W RPN9 het Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.199 2.60E-31 YEL034W HYP2 het Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
0.197 1.25E-30 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.189 5.25E-28 YDR303C RSC3 het Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p
0.182 1.72E-26 YDR047W HEM12 het Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.162 3.36E-21 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.156 1.32E-19 YOR117W RPT5 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.148 7.47E-18 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.144 5.45E-17 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.137 1.71E-15 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.124 5.05E-13 YCR052W RSC6 het Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
0.123 7.32E-13 YDR232W HEM1 het 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
0.122 1.57E-12 YBL105C PKC1 het Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)