HIPHOP chemogenomics database

YIL075C / RPN2

Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YIL075C / RPN2

Click on Significant Values for Screen Details

Top fitness defect scores for YIL075C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_1435	4092-0789	6.15	56.7 µM	3.86E-10	plasma membrane duress	2	3.77E-10	1.69
2	SGTC_2516	gossypol	5.65	71.5 µM	7.66E-9		4	7.91E-9	0.45
3	SGTC_674	0498-0004	5.26	451.0 µM	6.58E-8	heme requiring	7	7.04E-8	0.16
4	SGTC_2519	cryptotanshinone	5.12	88.0 µM	1.40E-7	heme biosynthesis & mitochondrial translocase	10	1.51E-7	0.31
5	SGTC_2336	9001819	5.08	198.7 µM	1.69E-7	heme biosynthesis & mitochondrial translocase	25	1.84E-7	0.00
6	SGTC_2575	plumbagin	5.00	510.0 nM	2.66E-7	superoxide	17	2.91E-7	0.12
7	SGTC_2301	7769476	4.39	200.0 µM	4.93E-6		10	5.62E-6	0.05
8	SGTC_123	0083-0120	4.27	66.7 µM	8.68E-6		10	9.96E-6	0.22
9	SGTC_1631	benzylvanillin	4.26	81.9 µM	8.87E-6	Golgi	5	1.02E-5	0.27
10	SGTC_584	0986-0246	4.20	15.4 µM	1.18E-5	heme requiring	23	1.36E-5	0.09
11	SGTC_1797	st048695	4.17	56.8 µM	1.33E-5	NEO1	13	1.53E-5	0.04
12	SGTC_1187	1391-0724	4.16	117.0 µM	1.37E-5	amide catabolism	3	1.58E-5	0.42
13	SGTC_2129	girgensonine	4.13	121.0 µM	1.56E-5	heme biosynthesis & mitochondrial translocase	21	1.80E-5	0.01
14	SGTC_3075	9116269	4.12	49.5 µM	1.64E-5		6	1.89E-5	0.27
15	SGTC_862	0452-0056	4.10	10.2 µM	1.82E-5	azole & statin	3	2.10E-5	0.78
16	SGTC_32	0928-0142	4.09	34.1 µM	1.84E-5	TSC3-RPN4	7	2.13E-5	0.61
17	SGTC_2517	totarol	4.09	10.0 µM	1.87E-5		4	2.17E-5	0.08
18	SGTC_177	1327-0068	4.07	8.4 µM	2.04E-5		21	2.36E-5	0.02
19	SGTC_2112	5488546	4.07	200.0 µM	2.07E-5	TSC3-RPN4	6	2.40E-5	0.05
20	SGTC_2400	5203494	4.05	178.7 µM	2.21E-5	RSC complex & mRNA processing	18	2.56E-5	0.00

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.225	7.56E-40	YDL205C	HEM3	het	Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.204	6.83E-33	YER021W	RPN3	het	Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.204	7.98E-33	YDR427W	RPN9	het	Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.199	2.60E-31	YEL034W	HYP2	het	Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
0.197	1.25E-30	YKL195W	MIA40	het	Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.189	5.25E-28	YDR303C	RSC3	het	Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p
0.182	1.72E-26	YDR047W	HEM12	het	Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.162	3.36E-21	YBR192W	RIM2	het	Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.156	1.32E-19	YOR117W	RPT5	het	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.148	7.47E-18	YGL048C	RPT6	het	ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.144	5.45E-17	YGL040C	HEM2	het	Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.137	1.71E-15	YMR203W	TOM40	het	Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.124	5.05E-13	YCR052W	RSC6	het	Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
0.123	7.32E-13	YDR232W	HEM1	het	5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
0.122	1.57E-12	YBL105C	PKC1	het	Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)