YIL095W / PRK1

Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL095W / PRK1

Click on Significant Values for Screen Details ID:SGTC_178|Compound:4092-0806|FD-Score:3.06|P-value:6.95E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.96|P-value:1.78E-5 ID:SGTC_281|Compound:1672-3440|FD-Score:3.11|P-value:5.82E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.15|P-value:5.02E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.35|P-value:2.37E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:3.13|P-value:5.42E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.72|P-value:5.27E-5 ID:SGTC_598|Compound:3937-0236|FD-Score:3.37|P-value:2.19E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:2.98|P-value:9.24E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.22|P-value:3.95E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:5.16|P-value:3.71E-8 ID:SGTC_907|Compound:3448-6986|FD-Score:3.48|P-value:1.42E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.71|P-value:4.39E-7 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.11|P-value:5.76E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:3.20|P-value:4.23E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:3.27|P-value:3.27E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.71|P-value:5.39E-5 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.03|P-value:7.95E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-3.41|P-value:1.86E-4 ID:SGTC_1767|Compound:st044992|FD-Score:3.56|P-value:1.03E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.54|P-value:1.12E-4 ID:SGTC_1852|Compound:st056254|FD-Score:3.24|P-value:3.61E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:-3.21|P-value:4.11E-4 ID:SGTC_1947|Compound:st076332|FD-Score:3.01|P-value:8.34E-4 ID:SGTC_1989|Compound:st071868|FD-Score:-3.02|P-value:8.30E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.13|P-value:5.42E-4 ID:SGTC_2012|Compound:4023501|FD-Score:5.10|P-value:5.13E-8 ID:SGTC_2028|Compound:5153890|FD-Score:-3.26|P-value:3.45E-4 ID:SGTC_2041|Compound:5107442|FD-Score:-3.28|P-value:3.14E-4 ID:SGTC_2046|Compound:4110841|FD-Score:-3.48|P-value:1.43E-4 ID:SGTC_2164|Compound:5685496|FD-Score:3.02|P-value:8.10E-4 ID:SGTC_2166|Compound:5933061|FD-Score:3.05|P-value:7.25E-4 ID:SGTC_2184|Compound:6075246|FD-Score:4.02|P-value:1.33E-5 ID:SGTC_2266|Compound:7929956|FD-Score:4.06|P-value:1.12E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.09|P-value:6.34E-4 ID:SGTC_2436|Compound:5633522|FD-Score:-3.04|P-value:7.64E-4 ID:SGTC_2477|Compound:5967686|FD-Score:5.08|P-value:5.68E-8 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.38|P-value:2.07E-4 ID:SGTC_2694|Compound:st077685|FD-Score:3.01|P-value:8.29E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.46|P-value:1.50E-4 ID:SGTC_2888|Compound:9058547|FD-Score:3.06|P-value:7.02E-4 ID:SGTC_2927|Compound:9011802|FD-Score:3.15|P-value:5.08E-4 ID:SGTC_3130|Compound:9127414|FD-Score:3.18|P-value:4.55E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.02|P-value:8.05E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.06|P-value:6.95E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.96|P-value:1.78E-5 ID:SGTC_281|Compound:1672-3440|FD-Score:3.11|P-value:5.82E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.15|P-value:5.02E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.35|P-value:2.37E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:3.13|P-value:5.42E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.72|P-value:5.27E-5 ID:SGTC_598|Compound:3937-0236|FD-Score:3.37|P-value:2.19E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:2.98|P-value:9.24E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:3.22|P-value:3.95E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:5.16|P-value:3.71E-8 ID:SGTC_907|Compound:3448-6986|FD-Score:3.48|P-value:1.42E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.71|P-value:4.39E-7 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.11|P-value:5.76E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:3.20|P-value:4.23E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:3.27|P-value:3.27E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.71|P-value:5.39E-5 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.03|P-value:7.95E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-3.41|P-value:1.86E-4 ID:SGTC_1767|Compound:st044992|FD-Score:3.56|P-value:1.03E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.54|P-value:1.12E-4 ID:SGTC_1852|Compound:st056254|FD-Score:3.24|P-value:3.61E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:-3.21|P-value:4.11E-4 ID:SGTC_1947|Compound:st076332|FD-Score:3.01|P-value:8.34E-4 ID:SGTC_1989|Compound:st071868|FD-Score:-3.02|P-value:8.30E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.13|P-value:5.42E-4 ID:SGTC_2012|Compound:4023501|FD-Score:5.10|P-value:5.13E-8 ID:SGTC_2028|Compound:5153890|FD-Score:-3.26|P-value:3.45E-4 ID:SGTC_2041|Compound:5107442|FD-Score:-3.28|P-value:3.14E-4 ID:SGTC_2046|Compound:4110841|FD-Score:-3.48|P-value:1.43E-4 ID:SGTC_2164|Compound:5685496|FD-Score:3.02|P-value:8.10E-4 ID:SGTC_2166|Compound:5933061|FD-Score:3.05|P-value:7.25E-4 ID:SGTC_2184|Compound:6075246|FD-Score:4.02|P-value:1.33E-5 ID:SGTC_2266|Compound:7929956|FD-Score:4.06|P-value:1.12E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.09|P-value:6.34E-4 ID:SGTC_2436|Compound:5633522|FD-Score:-3.04|P-value:7.64E-4 ID:SGTC_2477|Compound:5967686|FD-Score:5.08|P-value:5.68E-8 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.38|P-value:2.07E-4 ID:SGTC_2694|Compound:st077685|FD-Score:3.01|P-value:8.29E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.46|P-value:1.50E-4 ID:SGTC_2888|Compound:9058547|FD-Score:3.06|P-value:7.02E-4 ID:SGTC_2927|Compound:9011802|FD-Score:3.15|P-value:5.08E-4 ID:SGTC_3130|Compound:9127414|FD-Score:3.18|P-value:4.55E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.02|P-value:8.05E-4

Top fitness defect scores for YIL095W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_802 4112-3352 5.16 742.0 µM 3.71E-8 7 1.26E-7
2 SGTC_2012 4023501 5.10 200.0 µM 5.13E-8 10 1.69E-7
3 SGTC_2477 5967686 5.08 25.2 µM 5.68E-8 15 1.86E-7
4 SGTC_1005 4092-0690 4.71 4.4 µM 4.39E-7 plasma membrane duress 18 1.22E-6
5 SGTC_2266 7929956 4.06 200.0 µM 1.12E-5 29 2.43E-5
6 SGTC_2184 6075246 4.02 200.0 µM 1.33E-5 22 2.85E-5
7 SGTC_227 fluconazole 3.96 33.5 µM 1.78E-5 azole & statin 83 3.72E-5
8 SGTC_1350 1495-0454 3.71 146.0 µM 5.39E-5 37 1.03E-4
9 SGTC_1767 st044992 3.56 70.3 µM 1.03E-4 32 1.88E-4
10 SGTC_1801 st050089 3.54 28.4 µM 1.12E-4 NEO1-PIK1 142 2.03E-4
11 SGTC_907 3448-6986 3.48 75.5 µM 1.42E-4 42 2.54E-4
12 SGTC_2778 tioconazole 3.46 65.1 nM 1.50E-4 ergosterol depletion effects on membrane 80 2.66E-4
13 SGTC_2504 purpurogallin 3.38 52.3 µM 2.07E-4 RPP1 & pyrimidine depletion 37 3.58E-4
14 SGTC_598 3937-0236 3.37 20.2 µM 2.19E-4 ERG2 46 3.77E-4
15 SGTC_1275 0867-0008 3.27 21.1 µM 3.27E-4 29 5.46E-4
16 SGTC_1852 st056254 3.24 31.4 µM 3.61E-4 31 5.99E-4
17 SGTC_668 1682-0499 3.22 62.6 µM 3.95E-4 58 6.50E-4
18 SGTC_1269 0831-0796 3.20 3.0 µM 4.23E-4 60S ribosome export 37 6.94E-4
19 SGTC_3130 9127414 3.18 49.5 µM 4.55E-4 32 7.41E-4
20 SGTC_376 3970-0862 3.15 59.1 µM 5.02E-4 48 8.12E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.121 2.15E-12 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.086 5.37E-7 YIR019C FLO11 hom GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain
0.084 1.14E-6 YIR030C DCG1 hom Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain
0.084 1.17E-6 YGL122C NAB2 het Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress
0.081 2.31E-6 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.081 2.97E-6 YER051W JHD1 hom JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe
0.078 6.48E-6 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.077 7.81E-6 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.077 8.74E-6 YKL005C BYE1 hom Negative regulator of transcription elongation, contains a TFIIS-like domain and a PHD finger, multicopy suppressor of temperature-sensitive ess1 mutations, probably binds RNA polymerase II large subunit
0.076 1.14E-5 YJL098W SAP185 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication
0.075 1.26E-5 YDR093W DNF2 hom Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
0.075 1.35E-5 YDR160W SSY1 het Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
0.075 1.36E-5 YER153C PET122 hom Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
0.075 1.39E-5 YGL047W ALG13 het Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
0.074 2.02E-5 YLR374C_d YLR374C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W