YIL118W / RHO3

Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YIL118W / RHO3

Click on Significant Values for Screen Details ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.29|P-value:5.21E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.39|P-value:2.63E-4 ID:SGTC_252|Compound:miconazole|FD-Score:4.83|P-value:4.25E-7 ID:SGTC_439|Compound:amiodarone|FD-Score:3.64|P-value:9.70E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.14|P-value:1.19E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:3.44|P-value:2.15E-4 ID:SGTC_688|Compound:0527-0193|FD-Score:4.15|P-value:1.10E-5 ID:SGTC_689|Compound:0833-0039|FD-Score:4.38|P-value:3.96E-6 ID:SGTC_696|Compound:1560-0013|FD-Score:-3.62|P-value:1.51E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:3.38|P-value:2.68E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:6.83|P-value:1.79E-12 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.91|P-value:3.18E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.45|P-value:2.09E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.33|P-value:3.25E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.96|P-value:2.63E-5 ID:SGTC_2065|Compound:5221596|FD-Score:-3.23|P-value:6.44E-4 ID:SGTC_2156|Compound:5649887|FD-Score:-3.81|P-value:6.92E-5 ID:SGTC_2190|Compound:6049624|FD-Score:3.66|P-value:8.99E-5 ID:SGTC_2198|Compound:6589944|FD-Score:3.04|P-value:9.23E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.43|P-value:3.12E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.30|P-value:5.67E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.15|P-value:6.20E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-3.29|P-value:5.13E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-3.30|P-value:4.96E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-3.88|P-value:5.29E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-3.73|P-value:9.54E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.13|P-value:8.98E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-4.67|P-value:1.48E-6 ID:SGTC_2934|Compound:9036370|FD-Score:-3.31|P-value:4.71E-4 ID:SGTC_2962|Compound:9082311|FD-Score:4.17|P-value:1.02E-5 ID:SGTC_2976|Compound:9009005|FD-Score:-3.71|P-value:1.03E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-3.44|P-value:3.01E-4 ID:SGTC_3125|Compound:9127534|FD-Score:-3.57|P-value:1.82E-4 ID:SGTC_3212|Compound:9128307|FD-Score:3.46|P-value:2.02E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-3.28|P-value:5.28E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.67|P-value:8.58E-5 ID:SGTC_3273|Compound:9137859|FD-Score:3.37|P-value:2.79E-4 ID:SGTC_3278|Compound:9139605|FD-Score:4.74|P-value:6.50E-7 ID:SGTC_3301|Compound:9125354|FD-Score:3.10|P-value:7.50E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.29|P-value:5.21E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.39|P-value:2.63E-4 ID:SGTC_252|Compound:miconazole|FD-Score:4.83|P-value:4.25E-7 ID:SGTC_439|Compound:amiodarone|FD-Score:3.64|P-value:9.70E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.14|P-value:1.19E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:3.44|P-value:2.15E-4 ID:SGTC_688|Compound:0527-0193|FD-Score:4.15|P-value:1.10E-5 ID:SGTC_689|Compound:0833-0039|FD-Score:4.38|P-value:3.96E-6 ID:SGTC_696|Compound:1560-0013|FD-Score:-3.62|P-value:1.51E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:3.38|P-value:2.68E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:6.83|P-value:1.79E-12 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.91|P-value:3.18E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.45|P-value:2.09E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.33|P-value:3.25E-4 ID:SGTC_1747|Compound:st037496|FD-Score:3.96|P-value:2.63E-5 ID:SGTC_2065|Compound:5221596|FD-Score:-3.23|P-value:6.44E-4 ID:SGTC_2156|Compound:5649887|FD-Score:-3.81|P-value:6.92E-5 ID:SGTC_2190|Compound:6049624|FD-Score:3.66|P-value:8.99E-5 ID:SGTC_2198|Compound:6589944|FD-Score:3.04|P-value:9.23E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.43|P-value:3.12E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.30|P-value:5.67E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.15|P-value:6.20E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-3.29|P-value:5.13E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-3.30|P-value:4.96E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-3.88|P-value:5.29E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-3.73|P-value:9.54E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.13|P-value:8.98E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-4.67|P-value:1.48E-6 ID:SGTC_2934|Compound:9036370|FD-Score:-3.31|P-value:4.71E-4 ID:SGTC_2962|Compound:9082311|FD-Score:4.17|P-value:1.02E-5 ID:SGTC_2976|Compound:9009005|FD-Score:-3.71|P-value:1.03E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-3.44|P-value:3.01E-4 ID:SGTC_3125|Compound:9127534|FD-Score:-3.57|P-value:1.82E-4 ID:SGTC_3212|Compound:9128307|FD-Score:3.46|P-value:2.02E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-3.28|P-value:5.28E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.67|P-value:8.58E-5 ID:SGTC_3273|Compound:9137859|FD-Score:3.37|P-value:2.79E-4 ID:SGTC_3278|Compound:9139605|FD-Score:4.74|P-value:6.50E-7 ID:SGTC_3301|Compound:9125354|FD-Score:3.10|P-value:7.50E-4

Top fitness defect scores for YIL118W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1129 3453-1384 6.83 223.0 µM 1.79E-12 2 4.12E-12 0.26
2 SGTC_252 miconazole 4.83 1.3 nM 4.25E-7 ergosterol depletion effects on membrane 11 6.92E-7 0.08
3 SGTC_3278 9139605 4.74 49.5 µM 6.50E-7 6 1.05E-6 0.31
4 SGTC_689 0833-0039 4.38 98.0 µM 3.96E-6 ergosterol depletion effects on membrane 7 6.04E-6 0.14
5 SGTC_2249 haloperidol 4.30 116.4 µM 5.67E-6 ergosterol depletion effects on membrane 34 8.57E-6 0.01
6 SGTC_2962 9082311 4.17 13.2 µM 1.02E-5 8 1.51E-5 0.05
7 SGTC_688 0527-0193 4.15 473.0 µM 1.10E-5 fatty acid desaturase (OLE1) 4 1.63E-5 0.56
8 SGTC_450 aminopyrene 4.14 156.0 nM 1.19E-5 7 1.76E-5 0.69
9 SGTC_1747 st037496 3.96 66.6 µM 2.63E-5 6 3.79E-5 0.08
10 SGTC_1130 3454-2858 3.91 15.9 µM 3.18E-5 NEO1 13 4.57E-5 0.32
11 SGTC_3272 9137413 3.67 49.5 µM 8.58E-5 9 1.19E-4 0.00
12 SGTC_2190 6049624 3.66 200.0 µM 8.99E-5 2 1.25E-4 0.31
13 SGTC_439 amiodarone 3.64 34.4 µM 9.70E-5 NEO1-PIK1 9 1.35E-4 0.24
14 SGTC_3212 9128307 3.46 49.5 µM 2.02E-4 17 2.74E-4 0.03
15 SGTC_1293 1000-1278 3.45 195.0 µM 2.09E-4 13 2.83E-4 0.10
16 SGTC_637 1181-0519 3.44 7.3 µM 2.15E-4 fatty acid desaturase (OLE1) 15 2.90E-4 0.03
17 SGTC_237 nikkomycin 3.39 200.0 µM 2.63E-4 RSC complex & mRNA processing 13 3.54E-4 0.10
18 SGTC_748 0136-0228 3.38 77.9 µM 2.68E-4 redox potentiating 10 3.60E-4 0.10
19 SGTC_3273 9137859 3.37 49.5 µM 2.79E-4 7 3.75E-4 0.12
20 SGTC_1360 1598-0022 3.33 39.8 µM 3.25E-4 ERG2 18 4.34E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 4.53E-13 YCR094W CDC50 hom Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
0.116 1.63E-11 YDR143C SAN1 hom Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition
0.114 3.89E-11 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.109 2.83E-10 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.108 2.96E-10 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.108 3.27E-10 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.108 3.44E-10 YGR074W SMD1 het Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
0.106 7.11E-10 YBL005W PDR3 hom Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.106 7.20E-10 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.103 1.96E-9 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.101 4.15E-9 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.101 4.62E-9 YFL005W SEC4 het Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
0.100 5.49E-9 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.099 8.16E-9 YDL212W SHR3 het Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
0.098 1.52E-8 YLR171W_d YLR171W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data