YIL140W / AXL2

Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL140W / AXL2

Click on Significant Values for Screen Details ID:SGTC_151|Compound:3013-0144|FD-Score:3.13|P-value:8.42E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.95|P-value:3.66E-5 ID:SGTC_165|Compound:k038-0023|FD-Score:3.10|P-value:9.12E-4 ID:SGTC_251|Compound:melphalan|FD-Score:-3.46|P-value:2.88E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.80|P-value:6.96E-5 ID:SGTC_615|Compound:k087-0205|FD-Score:-3.56|P-value:1.97E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.56|P-value:1.73E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:7.55|P-value:1.95E-14 ID:SGTC_769|Compound:0302-0231|FD-Score:3.21|P-value:6.22E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:3.34|P-value:3.97E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-3.22|P-value:6.71E-4 ID:SGTC_1141|Compound:3253-0389|FD-Score:3.44|P-value:2.77E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:5.19|P-value:9.54E-8 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.60|P-value:1.69E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:-3.84|P-value:6.39E-5 ID:SGTC_1594|Compound:piperine|FD-Score:-3.21|P-value:6.97E-4 ID:SGTC_1717|Compound:st033143|FD-Score:3.65|P-value:1.25E-4 ID:SGTC_1914|Compound:st062367|FD-Score:5.01|P-value:2.50E-7 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.57|P-value:1.67E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.69|P-value:1.05E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.65|P-value:1.23E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:4.49|P-value:3.26E-6 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.39|P-value:3.64E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.12|P-value:8.62E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.18|P-value:6.96E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.58|P-value:1.60E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.32|P-value:4.20E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.30|P-value:4.52E-4 ID:SGTC_2399|Compound:5884133|FD-Score:6.37|P-value:8.73E-11 ID:SGTC_2429|Compound:5902844|FD-Score:-3.11|P-value:9.71E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-3.97|P-value:3.80E-5 ID:SGTC_2448|Compound:5185112|FD-Score:-3.13|P-value:9.20E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-3.46|P-value:2.83E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.47|P-value:2.47E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-3.20|P-value:7.13E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-3.11|P-value:9.74E-4 ID:SGTC_2979|Compound:9012552|FD-Score:3.35|P-value:3.87E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-3.23|P-value:6.45E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.13|P-value:8.42E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.95|P-value:3.66E-5 ID:SGTC_165|Compound:k038-0023|FD-Score:3.10|P-value:9.12E-4 ID:SGTC_251|Compound:melphalan|FD-Score:-3.46|P-value:2.88E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:3.80|P-value:6.96E-5 ID:SGTC_615|Compound:k087-0205|FD-Score:-3.56|P-value:1.97E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.56|P-value:1.73E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:7.55|P-value:1.95E-14 ID:SGTC_769|Compound:0302-0231|FD-Score:3.21|P-value:6.22E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:3.34|P-value:3.97E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-3.22|P-value:6.71E-4 ID:SGTC_1141|Compound:3253-0389|FD-Score:3.44|P-value:2.77E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:5.19|P-value:9.54E-8 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.60|P-value:1.69E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:-3.84|P-value:6.39E-5 ID:SGTC_1594|Compound:piperine|FD-Score:-3.21|P-value:6.97E-4 ID:SGTC_1717|Compound:st033143|FD-Score:3.65|P-value:1.25E-4 ID:SGTC_1914|Compound:st062367|FD-Score:5.01|P-value:2.50E-7 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.57|P-value:1.67E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.69|P-value:1.05E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.65|P-value:1.23E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:4.49|P-value:3.26E-6 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.39|P-value:3.64E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.12|P-value:8.62E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.18|P-value:6.96E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.58|P-value:1.60E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.32|P-value:4.20E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.30|P-value:4.52E-4 ID:SGTC_2399|Compound:5884133|FD-Score:6.37|P-value:8.73E-11 ID:SGTC_2429|Compound:5902844|FD-Score:-3.11|P-value:9.71E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-3.97|P-value:3.80E-5 ID:SGTC_2448|Compound:5185112|FD-Score:-3.13|P-value:9.20E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-3.46|P-value:2.83E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.47|P-value:2.47E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-3.20|P-value:7.13E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-3.11|P-value:9.74E-4 ID:SGTC_2979|Compound:9012552|FD-Score:3.35|P-value:3.87E-4 ID:SGTC_3263|Compound:9137018|FD-Score:-3.23|P-value:6.45E-4

Top fitness defect scores for YIL140W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_757 1066-0008 7.55 322.0 µM 1.95E-14 Golgi 1 2.20E-14
2 SGTC_2399 5884133 6.37 200.0 µM 8.73E-11 cell wall signaling 27 9.66E-11
3 SGTC_1274 0865-0114 5.19 173.0 µM 9.54E-8 6 1.03E-7
4 SGTC_1914 st062367 5.01 72.6 µM 2.50E-7 7 2.70E-7
5 SGTC_2131 toluylene red 4.49 5.3 µM 3.26E-6 60S ribosome export 11 3.49E-6
6 SGTC_158 k038-0087 3.95 26.4 µM 3.66E-5 ubiquinone biosynthesis & proteasome 141 3.89E-5
7 SGTC_508 roscovitine 3.80 141.0 µM 6.96E-5 9 7.38E-5
8 SGTC_2024 5144227 3.69 134.0 µM 1.05E-4 44 1.11E-4
9 SGTC_2085 5307070 3.65 144.1 µM 1.23E-4 superoxide 36 1.30E-4
10 SGTC_1717 st033143 3.65 58.0 µM 1.25E-4 56 1.32E-4
11 SGTC_2313 7726885 3.58 200.0 µM 1.60E-4 RPP1 & pyrimidine depletion 45 1.69E-4
12 SGTC_1951 sophoraflavanone G 3.57 9.2 µM 1.67E-4 RNA processing & uracil transport 40 1.77E-4
13 SGTC_752 1896-0090 3.56 130.0 µM 1.73E-4 TSC3-RPN4 25 1.83E-4
14 SGTC_2612 dictamnine 3.47 15.5 µM 2.47E-4 RNA pol III & RNase P/MRP 64 2.60E-4
15 SGTC_1141 3253-0389 3.44 52.6 µM 2.77E-4 16 2.93E-4
16 SGTC_2979 9012552 3.35 45.5 µM 3.87E-4 16 4.08E-4
17 SGTC_866 0495-0101 3.34 262.0 µM 3.97E-4 60S ribosome export 37 4.18E-4
18 SGTC_2347 9049783 3.32 174.8 µM 4.20E-4 50 4.43E-4
19 SGTC_2396 5342950 3.30 42.8 µM 4.52E-4 RSC complex & mRNA processing 75 4.76E-4
20 SGTC_769 0302-0231 3.21 76.0 µM 6.22E-4 96 6.54E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.136 2.58E-15 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.127 1.39E-13 YER009W NTF2 het Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
0.127 1.56E-13 YLR401C DUS3 hom Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
0.120 2.79E-12 YPL166W ATG29 hom Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.116 1.34E-11 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.116 1.96E-11 YOR233W KIN4 hom Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
0.105 1.17E-9 YJL183W MNN11 hom Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
0.101 4.75E-9 YBR058C UBP14 hom Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T
0.100 5.82E-9 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.099 7.98E-9 YNL308C KRI1 het Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
0.096 2.45E-8 YJL218W_p YJL218W_p hom Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene
0.095 3.47E-8 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.094 4.51E-8 YJL169W_d YJL169W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
0.091 1.36E-7 YLR028C ADE16 hom Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
0.090 1.73E-7 YAR027W UIP3 hom Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family