YIL158W / AIM20

Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL158W / AIM20

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:-3.29|P-value:7.79E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.27|P-value:8.43E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-4.08|P-value:4.31E-5 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.22|P-value:9.90E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:-3.56|P-value:3.16E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.60|P-value:2.51E-4 ID:SGTC_707|Compound:0865-0008|FD-Score:3.24|P-value:8.57E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-3.23|P-value:9.54E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:-3.34|P-value:6.59E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:-4.58|P-value:5.42E-6 ID:SGTC_874|Compound:0599-0792|FD-Score:-3.25|P-value:9.10E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:6.36|P-value:4.03E-10 ID:SGTC_927|Compound:2425-1185|FD-Score:4.67|P-value:3.11E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.23|P-value:8.72E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.66|P-value:2.01E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.53|P-value:3.17E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.93|P-value:7.30E-5 ID:SGTC_1635|Compound:st007035|FD-Score:4.46|P-value:8.07E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-3.66|P-value:2.18E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.68|P-value:1.84E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:5.56|P-value:3.92E-8 ID:SGTC_1897|Compound:st060201|FD-Score:3.30|P-value:7.01E-4 ID:SGTC_1961|Compound:st076550|FD-Score:3.61|P-value:2.38E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-4.07|P-value:4.59E-5 ID:SGTC_2031|Compound:5180455|FD-Score:3.55|P-value:2.96E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.30|P-value:6.99E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.38|P-value:5.82E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:4.19|P-value:2.53E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.59|P-value:2.59E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-4.68|P-value:3.38E-6 ID:SGTC_2841|Compound:9004761|FD-Score:-3.57|P-value:3.05E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-4.01|P-value:5.78E-5 ID:SGTC_2916|Compound:7875993|FD-Score:-3.92|P-value:8.10E-5 ID:SGTC_3016|Compound:9080866|FD-Score:5.55|P-value:4.00E-8 ID:SGTC_3131|Compound:9127911|FD-Score:-4.61|P-value:4.70E-6 ID:SGTC_3132|Compound:9126706|FD-Score:-4.30|P-value:1.76E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-3.67|P-value:2.08E-4 ID:SGTC_3274|Compound:9138298|FD-Score:3.35|P-value:5.95E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:-3.29|P-value:7.79E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.27|P-value:8.43E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-4.08|P-value:4.31E-5 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.22|P-value:9.90E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:-3.56|P-value:3.16E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.60|P-value:2.51E-4 ID:SGTC_707|Compound:0865-0008|FD-Score:3.24|P-value:8.57E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-3.23|P-value:9.54E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:-3.34|P-value:6.59E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:-4.58|P-value:5.42E-6 ID:SGTC_874|Compound:0599-0792|FD-Score:-3.25|P-value:9.10E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:6.36|P-value:4.03E-10 ID:SGTC_927|Compound:2425-1185|FD-Score:4.67|P-value:3.11E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.23|P-value:8.72E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.66|P-value:2.01E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.53|P-value:3.17E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.93|P-value:7.30E-5 ID:SGTC_1635|Compound:st007035|FD-Score:4.46|P-value:8.07E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-3.66|P-value:2.18E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.68|P-value:1.84E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:5.56|P-value:3.92E-8 ID:SGTC_1897|Compound:st060201|FD-Score:3.30|P-value:7.01E-4 ID:SGTC_1961|Compound:st076550|FD-Score:3.61|P-value:2.38E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-4.07|P-value:4.59E-5 ID:SGTC_2031|Compound:5180455|FD-Score:3.55|P-value:2.96E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.30|P-value:6.99E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.38|P-value:5.82E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:4.19|P-value:2.53E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.59|P-value:2.59E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-4.68|P-value:3.38E-6 ID:SGTC_2841|Compound:9004761|FD-Score:-3.57|P-value:3.05E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-4.01|P-value:5.78E-5 ID:SGTC_2916|Compound:7875993|FD-Score:-3.92|P-value:8.10E-5 ID:SGTC_3016|Compound:9080866|FD-Score:5.55|P-value:4.00E-8 ID:SGTC_3131|Compound:9127911|FD-Score:-4.61|P-value:4.70E-6 ID:SGTC_3132|Compound:9126706|FD-Score:-4.30|P-value:1.76E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-3.67|P-value:2.08E-4 ID:SGTC_3274|Compound:9138298|FD-Score:3.35|P-value:5.95E-4

Top fitness defect scores for YIL158W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_922 1866-0035 6.36 480.0 µM 4.03E-10 excess fatty acid 17 1.01E-10
2 SGTC_1878 temozolomide 5.56 1.0 mM 3.92E-8 2 1.36E-8
3 SGTC_3016 9080866 5.55 49.5 µM 4.00E-8 5 1.39E-8
4 SGTC_927 2425-1185 4.67 123.0 µM 3.11E-6 13 1.47E-6
5 SGTC_1635 st007035 4.46 42.4 µM 8.07E-6 186 4.08E-6
6 SGTC_2627 acetyl isogambogic acid 4.19 100.0 µM 2.53E-5 TSC3-RPN4 37 1.39E-5
7 SGTC_1532 4',5'-dihydroxyflavone 3.93 78.7 µM 7.30E-5 Golgi 21 4.30E-5
8 SGTC_1844 rapamycin 3.68 4.0 nM 1.84E-4 183 1.15E-4
9 SGTC_1293 1000-1278 3.66 195.0 µM 2.01E-4 16 1.27E-4
10 SGTC_1961 st076550 3.61 68.7 µM 2.38E-4 21 1.52E-4
11 SGTC_552 k242-0005 3.60 476.0 µM 2.51E-4 26 1.61E-4
12 SGTC_2779 methotrexate 3.59 400.0 µM 2.59E-4 36 1.66E-4
13 SGTC_2031 5180455 3.55 200.0 µM 2.96E-4 17 1.92E-4
14 SGTC_1358 1509-0022 3.53 2.5 µM 3.17E-4 21 2.06E-4
15 SGTC_3274 9138298 3.35 49.5 µM 5.95E-4 38 4.04E-4
16 SGTC_2286 7945697 3.30 125.0 µM 6.99E-4 82 4.80E-4
17 SGTC_1897 st060201 3.30 15.0 µM 7.01E-4 mitochondrial processes 36 4.82E-4
18 SGTC_707 0865-0008 3.24 204.0 µM 8.57E-4 DNA damage response 24 5.97E-4
19 SGTC_1260 0700-1617 3.23 7.8 µM 8.72E-4 calcium & mitochondrial duress 62 6.08E-4
20 SGTC_904 1141-0088 3.16 396.0 µM 0.00111 46 7.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.141 1.77E-16 YNR018W RCF2 hom Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
0.133 1.16E-14 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.128 1.02E-13 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.124 5.41E-13 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.113 5.92E-11 YGL191W COX13 hom Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.110 1.75E-10 YGR174C CBP4 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
0.108 3.50E-10 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.107 5.77E-10 YJL127C-B_p YJL127C-B_p hom Putative protein of unknown function; identified based on homology to the filamentous fungus, Ashbya gossypii
0.102 2.79E-9 YHR015W MIP6 hom Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
0.101 4.54E-9 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.101 5.11E-9 YDL172C_d YDL172C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.098 1.23E-8 YGL141W HUL5 hom Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD
0.097 1.58E-8 YDR032C PST2 hom Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.097 2.04E-8 YPL189C-A COA2 hom Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p
0.097 2.09E-8 YIL065C FIS1 hom Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation