HIPHOP chemogenomics database
YIR005W / IST3 
Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YIR005W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.108 | 4.01E-10 | YGL246C | RAI1 | hom | Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z |
| 0.106 | 6.59E-10 | YBR082C | UBC4 | hom | Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication |
| 0.104 | 1.60E-9 | YAL033W | POP5 | het | Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs |
| 0.103 | 1.94E-9 | YOR080W | DIA2 | hom | Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase |
| 0.100 | 5.67E-9 | YML057C-A_d | YML057C-A_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W |
| 0.095 | 3.01E-8 | YKR076W | ECM4 | hom | Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
| 0.095 | 3.53E-8 | YGL158W | RCK1 | hom | Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication |
| 0.088 | 3.62E-7 | YJR056C | YJR056C | hom | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress |
| 0.087 | 4.92E-7 | YLR037C | PAU23 | hom | Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth |
| 0.086 | 6.17E-7 | YNL305C | BXI1 | hom | Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p |
| 0.085 | 8.28E-7 | YDR253C | MET32 | hom | Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication |
| 0.084 | 1.17E-6 | YDR248C_p | YDR248C_p | hom | Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1 |
| 0.077 | 7.12E-6 | YCR019W | MAK32 | hom | Protein necessary for structural stability of L-A double-stranded RNA-containing particles |
| 0.076 | 1.00E-5 | YBR116C_d | YBR116C_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 |
| 0.075 | 1.39E-5 | YLL051C | FRE6 | hom | Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels |