YIR014W_p

Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene

Zygosity: Homozygous strain
fixedexpanded
Profile for YIR014W / YIR014W

Click on Significant Values for Screen Details ID:SGTC_130|Compound:0136-0219|FD-Score:3.61|P-value:6.69E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:-3.11|P-value:5.71E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-4.45|P-value:1.56E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:3.03|P-value:6.68E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.34|P-value:2.00E-4 ID:SGTC_389|Compound:tpck|FD-Score:4.22|P-value:4.07E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.17|P-value:4.47E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.04|P-value:6.44E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.07|P-value:5.62E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:-3.33|P-value:2.46E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.07|P-value:5.62E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.06|P-value:5.94E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-2.99|P-value:8.69E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.44|P-value:1.33E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.87|P-value:2.10E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.72|P-value:4.92E-5 ID:SGTC_1476|Compound:4112-3294|FD-Score:3.84|P-value:2.40E-5 ID:SGTC_1499|Compound:4480-0877|FD-Score:4.61|P-value:5.34E-7 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:2.94|P-value:9.20E-4 ID:SGTC_1567|Compound:st069303|FD-Score:2.97|P-value:8.34E-4 ID:SGTC_1704|Compound:st028805|FD-Score:2.99|P-value:7.58E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-4.25|P-value:4.18E-6 ID:SGTC_1853|Compound:st056710|FD-Score:-4.08|P-value:9.59E-6 ID:SGTC_1857|Compound:st056007|FD-Score:-4.18|P-value:5.88E-6 ID:SGTC_1958|Compound:st076513|FD-Score:2.92|P-value:9.90E-4 ID:SGTC_2010|Compound:4012422|FD-Score:3.78|P-value:3.12E-5 ID:SGTC_2019|Compound:4016004|FD-Score:-3.39|P-value:1.95E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-3.73|P-value:4.73E-5 ID:SGTC_2148|Compound:5546263|FD-Score:2.92|P-value:9.69E-4 ID:SGTC_2266|Compound:7929956|FD-Score:-3.36|P-value:2.16E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.99|P-value:1.19E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:3.15|P-value:4.26E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.60|P-value:7.06E-5 ID:SGTC_2625|Compound:euphol|FD-Score:3.30|P-value:2.35E-4 ID:SGTC_2860|Compound:9025725|FD-Score:3.58|P-value:7.51E-5 ID:SGTC_2870|Compound:9038528|FD-Score:2.94|P-value:9.17E-4 ID:SGTC_3175|Compound:9106517|FD-Score:-3.01|P-value:8.17E-4 ID:SGTC_3182|Compound:9108849|FD-Score:-3.65|P-value:6.68E-5 ID:SGTC_3193|Compound:9111931|FD-Score:-3.08|P-value:6.43E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:3.61|P-value:6.69E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:-3.11|P-value:5.71E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-4.45|P-value:1.56E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:3.03|P-value:6.68E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.34|P-value:2.00E-4 ID:SGTC_389|Compound:tpck|FD-Score:4.22|P-value:4.07E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.17|P-value:4.47E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.04|P-value:6.44E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.07|P-value:5.62E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:-3.33|P-value:2.46E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.07|P-value:5.62E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.06|P-value:5.94E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-2.99|P-value:8.69E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.44|P-value:1.33E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.87|P-value:2.10E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.72|P-value:4.92E-5 ID:SGTC_1476|Compound:4112-3294|FD-Score:3.84|P-value:2.40E-5 ID:SGTC_1499|Compound:4480-0877|FD-Score:4.61|P-value:5.34E-7 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:2.94|P-value:9.20E-4 ID:SGTC_1567|Compound:st069303|FD-Score:2.97|P-value:8.34E-4 ID:SGTC_1704|Compound:st028805|FD-Score:2.99|P-value:7.58E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-4.25|P-value:4.18E-6 ID:SGTC_1853|Compound:st056710|FD-Score:-4.08|P-value:9.59E-6 ID:SGTC_1857|Compound:st056007|FD-Score:-4.18|P-value:5.88E-6 ID:SGTC_1958|Compound:st076513|FD-Score:2.92|P-value:9.90E-4 ID:SGTC_2010|Compound:4012422|FD-Score:3.78|P-value:3.12E-5 ID:SGTC_2019|Compound:4016004|FD-Score:-3.39|P-value:1.95E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-3.73|P-value:4.73E-5 ID:SGTC_2148|Compound:5546263|FD-Score:2.92|P-value:9.69E-4 ID:SGTC_2266|Compound:7929956|FD-Score:-3.36|P-value:2.16E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.99|P-value:1.19E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:3.15|P-value:4.26E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:3.60|P-value:7.06E-5 ID:SGTC_2625|Compound:euphol|FD-Score:3.30|P-value:2.35E-4 ID:SGTC_2860|Compound:9025725|FD-Score:3.58|P-value:7.51E-5 ID:SGTC_2870|Compound:9038528|FD-Score:2.94|P-value:9.17E-4 ID:SGTC_3175|Compound:9106517|FD-Score:-3.01|P-value:8.17E-4 ID:SGTC_3182|Compound:9108849|FD-Score:-3.65|P-value:6.68E-5 ID:SGTC_3193|Compound:9111931|FD-Score:-3.08|P-value:6.43E-4

Top fitness defect scores for YIR014W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1499 4480-0877 4.61 67.3 µM 5.34E-7 3 1.98E-6
2 SGTC_1256 0570-0116 4.44 285.0 µM 1.33E-6 10 4.51E-6
3 SGTC_389 tpck 4.22 16.7 µM 4.07E-6 26 1.23E-5
4 SGTC_2432 5630864 3.99 12.2 µM 1.19E-5 20 3.26E-5
5 SGTC_1260 0700-1617 3.87 7.8 µM 2.10E-5 calcium & mitochondrial duress 31 5.42E-5
6 SGTC_1476 4112-3294 3.84 3.3 µM 2.40E-5 60S ribosome export 16 6.11E-5
7 SGTC_2010 4012422 3.78 159.0 µM 3.12E-5 26 7.76E-5
8 SGTC_130 0136-0219 3.61 337.7 µM 6.69E-5 31 1.54E-4
9 SGTC_2619 obliquin 3.60 87.9 µM 7.06E-5 cell wall 168 1.62E-4
10 SGTC_2860 9025725 3.58 39.0 µM 7.51E-5 RPP1 & pyrimidine depletion 18 1.71E-4
11 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 3.34 4.6 µM 2.00E-4 heat shock/prefoldin 59 4.15E-4
12 SGTC_2625 euphol 3.30 100.0 µM 2.35E-4 60 4.80E-4
13 SGTC_2605 digitonin 3.15 300.0 nM 4.26E-4 68 8.21E-4
14 SGTC_933 2914-0560 3.07 114.0 µM 5.62E-4 68 0.00105
15 SGTC_958 1222-0007 3.07 33.0 µM 5.62E-4 66 0.00106
16 SGTC_967 1306-0166 3.06 352.0 µM 5.94E-4 79 0.00111
17 SGTC_551 k048-0028 3.04 34.9 µM 6.44E-4 61 0.00119
18 SGTC_158 k038-0087 3.03 26.4 µM 6.68E-4 ubiquinone biosynthesis & proteasome 209 0.00123
19 SGTC_1704 st028805 2.99 70.8 µM 7.58E-4 41 0.00138
20 SGTC_1567 st069303 2.97 68.4 µM 8.34E-4 mitochondrial processes 74 0.00151

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.173 5.28E-24 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.142 1.15E-16 YGR209C TRX2 hom Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
0.140 3.22E-16 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.137 1.98E-15 YJL118W YJL118W hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein
0.130 3.91E-14 YDR191W HST4 hom Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
0.119 3.89E-12 YDR107C TMN2 hom Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments
0.118 8.19E-12 YPL121C MEI5 hom Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p
0.116 1.63E-11 YJR031C GEA1 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
0.113 4.83E-11 YGL067W NPY1 hom NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
0.106 6.49E-10 YNL339C YRF1-6 hom Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
0.105 1.20E-9 YOR050C_d YOR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.105 1.22E-9 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.104 1.27E-9 YDL210W UGA4 hom Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
0.103 2.15E-9 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.102 3.68E-9 YIL010W DOT5 hom Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth