YIR033W / MGA2

ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting

Zygosity: Homozygous strain
fixedexpanded
Profile for YIR033W / MGA2

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:-4.13|P-value:2.01E-4 ID:SGTC_100|Compound:0086-0128|FD-Score:3.53|P-value:2.99E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.54|P-value:2.91E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:-3.75|P-value:7.14E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:4.40|P-value:1.21E-5 ID:SGTC_209|Compound:4077-0173|FD-Score:3.58|P-value:2.56E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-5.56|P-value:6.44E-7 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.64|P-value:2.10E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-6.11|P-value:4.56E-8 ID:SGTC_1361|Compound:1608-0041|FD-Score:-4.19|P-value:1.61E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.58|P-value:2.54E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.92|P-value:7.63E-5 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.64|P-value:2.04E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.17|P-value:9.53E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.89|P-value:8.56E-5 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:4.06|P-value:4.64E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.92|P-value:4.09E-4 ID:SGTC_1657|Compound:st012939|FD-Score:4.69|P-value:3.60E-6 ID:SGTC_1886|Compound:st057356|FD-Score:4.21|P-value:2.52E-5 ID:SGTC_2166|Compound:5933061|FD-Score:-8.24|P-value:1.71E-13 ID:SGTC_2237|Compound:6667747|FD-Score:-4.64|P-value:3.13E-5 ID:SGTC_2238|Compound:6671620|FD-Score:-5.92|P-value:1.19E-7 ID:SGTC_2330|Compound:7979373|FD-Score:3.79|P-value:1.23E-4 ID:SGTC_2341|Compound:7987486|FD-Score:-4.20|P-value:1.59E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-3.81|P-value:5.84E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-3.82|P-value:5.71E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:-4.83|P-value:1.49E-5 ID:SGTC_2551|Compound:apiole|FD-Score:-6.08|P-value:5.33E-8 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-4.53|P-value:4.76E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.87|P-value:9.35E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:-5.69|P-value:3.63E-7 ID:SGTC_2695|Compound:st077686|FD-Score:4.51|P-value:7.81E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.08|P-value:4.22E-5 ID:SGTC_2832|Compound:7992762|FD-Score:3.58|P-value:2.59E-4 ID:SGTC_2982|Compound:9019404|FD-Score:-4.09|P-value:2.31E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-4.03|P-value:2.79E-4 ID:SGTC_3028|Compound:9087778|FD-Score:3.32|P-value:6.08E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-6.10|P-value:4.94E-8 ID:SGTC_3132|Compound:9126706|FD-Score:3.44|P-value:4.02E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-4.21|P-value:1.54E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.69|P-value:8.63E-4 ID:SGTC_3318|Compound:9139014|FD-Score:-4.56|P-value:4.33E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-4.13|P-value:2.01E-4 ID:SGTC_100|Compound:0086-0128|FD-Score:3.53|P-value:2.99E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.54|P-value:2.91E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:-3.75|P-value:7.14E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:4.40|P-value:1.21E-5 ID:SGTC_209|Compound:4077-0173|FD-Score:3.58|P-value:2.56E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-5.56|P-value:6.44E-7 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.64|P-value:2.10E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-6.11|P-value:4.56E-8 ID:SGTC_1361|Compound:1608-0041|FD-Score:-4.19|P-value:1.61E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.58|P-value:2.54E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.92|P-value:7.63E-5 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.64|P-value:2.04E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.17|P-value:9.53E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.89|P-value:8.56E-5 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:4.06|P-value:4.64E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.92|P-value:4.09E-4 ID:SGTC_1657|Compound:st012939|FD-Score:4.69|P-value:3.60E-6 ID:SGTC_1886|Compound:st057356|FD-Score:4.21|P-value:2.52E-5 ID:SGTC_2166|Compound:5933061|FD-Score:-8.24|P-value:1.71E-13 ID:SGTC_2237|Compound:6667747|FD-Score:-4.64|P-value:3.13E-5 ID:SGTC_2238|Compound:6671620|FD-Score:-5.92|P-value:1.19E-7 ID:SGTC_2330|Compound:7979373|FD-Score:3.79|P-value:1.23E-4 ID:SGTC_2341|Compound:7987486|FD-Score:-4.20|P-value:1.59E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-3.81|P-value:5.84E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-3.82|P-value:5.71E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:-4.83|P-value:1.49E-5 ID:SGTC_2551|Compound:apiole|FD-Score:-6.08|P-value:5.33E-8 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-4.53|P-value:4.76E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.87|P-value:9.35E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:-5.69|P-value:3.63E-7 ID:SGTC_2695|Compound:st077686|FD-Score:4.51|P-value:7.81E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.08|P-value:4.22E-5 ID:SGTC_2832|Compound:7992762|FD-Score:3.58|P-value:2.59E-4 ID:SGTC_2982|Compound:9019404|FD-Score:-4.09|P-value:2.31E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-4.03|P-value:2.79E-4 ID:SGTC_3028|Compound:9087778|FD-Score:3.32|P-value:6.08E-4 ID:SGTC_3029|Compound:9088683|FD-Score:-6.10|P-value:4.94E-8 ID:SGTC_3132|Compound:9126706|FD-Score:3.44|P-value:4.02E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-4.21|P-value:1.54E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.69|P-value:8.63E-4 ID:SGTC_3318|Compound:9139014|FD-Score:-4.56|P-value:4.33E-5

Top fitness defect scores for YIR033W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1657 st012939 4.69 43.4 µM 3.60E-6 4 1.35E-6
2 SGTC_2695 st077686 4.51 52.0 µM 7.81E-6 24 3.28E-6
3 SGTC_208 k072-0098 4.40 12.4 µM 1.21E-5 heme biosynthesis & mitochondrial translocase 31 5.39E-6
4 SGTC_1886 st057356 4.21 49.9 µM 2.52E-5 60S ribosome export 11 1.25E-5
5 SGTC_2759 disulfiram 4.08 3.4 µM 4.22E-5 24 2.24E-5
6 SGTC_1595 6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 4.06 67.5 µM 4.64E-5 33 2.50E-5
7 SGTC_1453 4237-0541 3.92 195.0 µM 7.63E-5 heme biosynthesis & mitochondrial translocase 28 4.39E-5
8 SGTC_1594 piperine 3.89 70.1 µM 8.56E-5 14 5.01E-5
9 SGTC_2575 plumbagin 3.87 510.0 nM 9.35E-5 superoxide 48 5.53E-5
10 SGTC_2330 7979373 3.79 200.0 µM 1.23E-4 60S ribosome export 40 7.54E-5
11 SGTC_1511 0906-3109 3.64 28.5 µM 2.04E-4 excess fatty acid 66 1.34E-4
12 SGTC_1045 1348-1485 3.64 165.0 µM 2.10E-4 45 1.38E-4
13 SGTC_1386 3228-0095 3.58 33.8 µM 2.54E-4 24 1.71E-4
14 SGTC_209 4077-0173 3.58 175.6 µM 2.56E-4 13 1.73E-4
15 SGTC_2832 7992762 3.58 52.0 µM 2.59E-4 43 1.75E-4
16 SGTC_153 4077-0274 3.54 519.7 µM 2.91E-4 27 2.00E-4
17 SGTC_100 0086-0128 3.53 49.6 µM 2.99E-4 copper-dependent oxidative stress 73 2.06E-4
18 SGTC_3132 9126706 3.44 49.5 µM 4.02E-4 44 2.87E-4
19 SGTC_3028 9087778 3.32 49.5 µM 6.08E-4 34 4.58E-4
20 SGTC_1588 farnesol 3.17 29.9 µM 9.53E-4 37 7.57E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.87E-11 YMR038C CCS1 hom Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
0.114 3.41E-11 YNL220W ADE12 hom Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence
0.099 9.80E-9 YFL045C SEC53 het Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
0.099 1.05E-8 YBL099W ATP1 hom Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
0.092 8.01E-8 YML091C RPM2 het Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
0.092 1.05E-7 YLR262C YPT6 hom Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
0.090 1.71E-7 YKL206C ADD66 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.080 3.75E-6 YDR202C RAV2 hom Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
0.079 4.29E-6 YNL170W_d YNL170W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.077 7.48E-6 YNR013C PHO91 hom Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
0.077 7.61E-6 YFL034C-B MOB2 het Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p
0.075 1.43E-5 YNL080C EOS1 hom Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene
0.074 1.72E-5 YBR012C_d YBR012C_d hom Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p
0.073 2.62E-5 YIL041W GVP36 hom BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis
0.072 2.65E-5 YPR036W VMA13 hom Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase