YIR034C / LYS1

Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity

Zygosity: Homozygous strain
fixedexpanded
Profile for YIR034C / LYS1

Click on Significant Values for Screen Details ID:SGTC_94|Compound:0334-0113|FD-Score:3.71|P-value:2.03E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.41|P-value:5.89E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:-3.92|P-value:7.26E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:3.33|P-value:7.81E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:4.37|P-value:1.58E-5 ID:SGTC_1021|Compound:4476-0760|FD-Score:6.20|P-value:1.55E-9 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.37|P-value:1.54E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:4.46|P-value:1.07E-5 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.79|P-value:1.56E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:4.15|P-value:3.82E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:5.69|P-value:2.72E-8 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.70|P-value:3.70E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.44|P-value:5.37E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.57|P-value:3.43E-4 ID:SGTC_1673|Compound:st016611|FD-Score:4.39|P-value:1.40E-5 ID:SGTC_1846|Compound:st056244|FD-Score:-3.92|P-value:6.99E-5 ID:SGTC_2017|Compound:5132927|FD-Score:3.78|P-value:1.61E-4 ID:SGTC_2078|Compound:5212016|FD-Score:-3.32|P-value:6.18E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-3.86|P-value:9.03E-5 ID:SGTC_2268|Compound:7933382|FD-Score:-3.18|P-value:9.89E-4 ID:SGTC_2389|Compound:9091542|FD-Score:3.48|P-value:4.70E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-3.35|P-value:5.62E-4 ID:SGTC_2482|Compound:5981709|FD-Score:5.99|P-value:5.19E-9 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.65|P-value:2.53E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:3.47|P-value:4.88E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.52|P-value:3.18E-4 ID:SGTC_2899|Compound:9053889|FD-Score:-3.27|P-value:7.38E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.66|P-value:1.89E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.84|P-value:9.51E-5 ID:SGTC_3206|Compound:9114350|FD-Score:4.42|P-value:1.26E-5 ID:SGTC_94|Compound:0334-0113|FD-Score:3.71|P-value:2.03E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.41|P-value:5.89E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:-3.92|P-value:7.26E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:3.33|P-value:7.81E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:4.37|P-value:1.58E-5 ID:SGTC_1021|Compound:4476-0760|FD-Score:6.20|P-value:1.55E-9 ID:SGTC_1023|Compound:4482-3888|FD-Score:4.37|P-value:1.54E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:4.46|P-value:1.07E-5 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.79|P-value:1.56E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:4.15|P-value:3.82E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:5.69|P-value:2.72E-8 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.70|P-value:3.70E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.44|P-value:5.37E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.57|P-value:3.43E-4 ID:SGTC_1673|Compound:st016611|FD-Score:4.39|P-value:1.40E-5 ID:SGTC_1846|Compound:st056244|FD-Score:-3.92|P-value:6.99E-5 ID:SGTC_2017|Compound:5132927|FD-Score:3.78|P-value:1.61E-4 ID:SGTC_2078|Compound:5212016|FD-Score:-3.32|P-value:6.18E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-3.86|P-value:9.03E-5 ID:SGTC_2268|Compound:7933382|FD-Score:-3.18|P-value:9.89E-4 ID:SGTC_2389|Compound:9091542|FD-Score:3.48|P-value:4.70E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-3.35|P-value:5.62E-4 ID:SGTC_2482|Compound:5981709|FD-Score:5.99|P-value:5.19E-9 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.65|P-value:2.53E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:3.47|P-value:4.88E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.52|P-value:3.18E-4 ID:SGTC_2899|Compound:9053889|FD-Score:-3.27|P-value:7.38E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.66|P-value:1.89E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.84|P-value:9.51E-5 ID:SGTC_3206|Compound:9114350|FD-Score:4.42|P-value:1.26E-5

Top fitness defect scores for YIR034C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1021 4476-0760 6.20 85.3 µM 1.55E-9 3 2.81E-10
2 SGTC_2482 5981709 5.99 142.7 µM 5.19E-9 PDR1 8 1.05E-9
3 SGTC_1356 1502-0781 5.69 85.8 µM 2.72E-8 fatty acid desaturase (OLE1) 4 6.32E-9
4 SGTC_1357 1503-1303 4.70 39.5 µM 3.70E-6 15 1.31E-6
5 SGTC_1108 betulinic acid 4.46 729.1 nM 1.07E-5 8 4.15E-6
6 SGTC_3206 9114350 4.42 49.5 µM 1.26E-5 9 4.96E-6
7 SGTC_1673 st016611 4.39 27.3 µM 1.40E-5 32 5.57E-6
8 SGTC_1023 4482-3888 4.37 16.0 µM 1.54E-5 TSC3-RPN4 7 6.19E-6
9 SGTC_995 3931-2021 4.37 34.6 µM 1.58E-5 RPP1 & pyrimidine depletion 15 6.35E-6
10 SGTC_1276 0871-0068 4.15 94.2 µM 3.82E-5 15 1.66E-5
11 SGTC_1117 retinoic acid 3.79 1.1 µM 1.56E-4 15 7.64E-5
12 SGTC_2017 5132927 3.78 60.6 µM 1.61E-4 35 7.93E-5
13 SGTC_94 0334-0113 3.71 514.8 µM 2.03E-4 26 1.02E-4
14 SGTC_2538 3',7'-epoxycaryophyllan-6-one 3.65 100.0 µM 2.53E-4 32 1.29E-4
15 SGTC_1473 k844-0725 3.57 134.0 µM 3.43E-4 25 1.80E-4
16 SGTC_2389 9091542 3.48 195.5 µM 4.70E-4 iron homeostasis 51 2.54E-4
17 SGTC_2665 lovastatin 3.47 100.0 µM 4.88E-4 26 2.64E-4
18 SGTC_1374 2889-5571 3.44 141.0 µM 5.37E-4 RSC complex & mRNA processing 65 2.93E-4
19 SGTC_612 0302-0167 3.41 22.4 µM 5.89E-4 copper-dependent oxidative stress 61 3.24E-4
20 SGTC_814 3342-0569 3.33 96.0 µM 7.81E-4 43 4.40E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.096 2.81E-8 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.084 1.17E-6 YML005W TRM12 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA
0.081 2.28E-6 YEL058W PCM1 het Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
0.074 1.84E-5 YGL145W TIP20 het Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
0.074 1.86E-5 YDR510W SMT3 het Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
0.073 2.54E-5 YNL113W RPC19 het RNA polymerase subunit AC19, common to RNA polymerases I and III
0.072 3.05E-5 YFL044C OTU1 hom Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
0.072 3.08E-5 YPR093C ASR1 hom Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein
0.072 3.26E-5 YDR018C_p YDR018C_p hom Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication
0.071 3.89E-5 YOR056C NOB1 het Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
0.070 4.43E-5 YLR210W CLB4 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation
0.069 6.46E-5 YDR344C_d YDR344C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.068 7.50E-5 YGL128C CWC23 het Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p
0.068 8.88E-5 YDR368W YPR1 hom NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication
0.067 9.73E-5 YBL094C_d YBL094C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W