YJL001W / PRE3

Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL001W / PRE3

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:6.77|P-value:1.72E-10 ID:SGTC_137|Compound:0438-0332|FD-Score:7.72|P-value:4.24E-13 ID:SGTC_142|Compound:0335-0849|FD-Score:3.91|P-value:1.42E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.49|P-value:1.54E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:3.56|P-value:4.81E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.43|P-value:7.67E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.84|P-value:1.84E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.51|P-value:5.62E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-4.72|P-value:6.37E-6 ID:SGTC_837|Compound:0091-0265|FD-Score:4.12|P-value:6.51E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.30|P-value:3.37E-5 ID:SGTC_929|Compound:2805-0021|FD-Score:3.43|P-value:7.28E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.35|P-value:9.40E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.66|P-value:3.38E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:-3.39|P-value:8.90E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:-3.88|P-value:1.67E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.59|P-value:4.55E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.51|P-value:5.97E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:-3.58|P-value:4.73E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.95|P-value:1.33E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:3.81|P-value:2.06E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.83|P-value:1.93E-4 ID:SGTC_1780|Compound:st038448|FD-Score:4.10|P-value:7.13E-5 ID:SGTC_2025|Compound:5147754|FD-Score:-3.87|P-value:1.75E-4 ID:SGTC_2248|Compound:tofa|FD-Score:-5.46|P-value:2.24E-7 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.98|P-value:1.10E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:14.80|P-value:4.81E-43 ID:SGTC_2257|Compound:7960413|FD-Score:-3.97|P-value:1.23E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.49|P-value:6.44E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.42|P-value:7.99E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-6.20|P-value:4.92E-9 ID:SGTC_2375|Compound:9072683|FD-Score:-3.57|P-value:4.93E-4 ID:SGTC_2380|Compound:9035439|FD-Score:-3.51|P-value:5.89E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.94|P-value:1.35E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:4.36|P-value:2.66E-5 ID:SGTC_2430|Compound:5571206|FD-Score:-4.50|P-value:1.60E-5 ID:SGTC_2435|Compound:5706476|FD-Score:-3.75|P-value:2.69E-4 ID:SGTC_2855|Compound:9016525|FD-Score:-3.45|P-value:7.20E-4 ID:SGTC_2857|Compound:9024691|FD-Score:4.87|P-value:3.21E-6 ID:SGTC_2898|Compound:9056270|FD-Score:-3.39|P-value:8.82E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-5.30|P-value:4.95E-7 ID:SGTC_3062|Compound:9077494|FD-Score:-3.69|P-value:3.25E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-6.80|P-value:1.62E-10 ID:SGTC_3214|Compound:9129694|FD-Score:-4.49|P-value:1.69E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-4.08|P-value:8.08E-5 ID:SGTC_3278|Compound:9139605|FD-Score:4.07|P-value:7.93E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:6.77|P-value:1.72E-10 ID:SGTC_137|Compound:0438-0332|FD-Score:7.72|P-value:4.24E-13 ID:SGTC_142|Compound:0335-0849|FD-Score:3.91|P-value:1.42E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.49|P-value:1.54E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:3.56|P-value:4.81E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.43|P-value:7.67E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.84|P-value:1.84E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.51|P-value:5.62E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:-4.72|P-value:6.37E-6 ID:SGTC_837|Compound:0091-0265|FD-Score:4.12|P-value:6.51E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.30|P-value:3.37E-5 ID:SGTC_929|Compound:2805-0021|FD-Score:3.43|P-value:7.28E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.35|P-value:9.40E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.66|P-value:3.38E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:-3.39|P-value:8.90E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:-3.88|P-value:1.67E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.59|P-value:4.55E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.51|P-value:5.97E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:-3.58|P-value:4.73E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.95|P-value:1.33E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:3.81|P-value:2.06E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.83|P-value:1.93E-4 ID:SGTC_1780|Compound:st038448|FD-Score:4.10|P-value:7.13E-5 ID:SGTC_2025|Compound:5147754|FD-Score:-3.87|P-value:1.75E-4 ID:SGTC_2248|Compound:tofa|FD-Score:-5.46|P-value:2.24E-7 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.98|P-value:1.10E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:14.80|P-value:4.81E-43 ID:SGTC_2257|Compound:7960413|FD-Score:-3.97|P-value:1.23E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.49|P-value:6.44E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.42|P-value:7.99E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-6.20|P-value:4.92E-9 ID:SGTC_2375|Compound:9072683|FD-Score:-3.57|P-value:4.93E-4 ID:SGTC_2380|Compound:9035439|FD-Score:-3.51|P-value:5.89E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.94|P-value:1.35E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:4.36|P-value:2.66E-5 ID:SGTC_2430|Compound:5571206|FD-Score:-4.50|P-value:1.60E-5 ID:SGTC_2435|Compound:5706476|FD-Score:-3.75|P-value:2.69E-4 ID:SGTC_2855|Compound:9016525|FD-Score:-3.45|P-value:7.20E-4 ID:SGTC_2857|Compound:9024691|FD-Score:4.87|P-value:3.21E-6 ID:SGTC_2898|Compound:9056270|FD-Score:-3.39|P-value:8.82E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-5.30|P-value:4.95E-7 ID:SGTC_3062|Compound:9077494|FD-Score:-3.69|P-value:3.25E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-6.80|P-value:1.62E-10 ID:SGTC_3214|Compound:9129694|FD-Score:-4.49|P-value:1.69E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-4.08|P-value:8.08E-5 ID:SGTC_3278|Compound:9139605|FD-Score:4.07|P-value:7.93E-5

Top fitness defect scores for YJL001W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2250 alverine citrate 14.80 130.0 µM 4.81E-43 3 7.69E-50 1.28
2 SGTC_137 0438-0332 7.72 85.4 µM 4.24E-13 ubiquinone biosynthesis & proteasome 12 5.85E-15 0.43
3 SGTC_64 000l-0018 6.77 272.1 µM 1.72E-10 ubiquinone biosynthesis & proteasome 26 6.31E-12 0.35
4 SGTC_2857 9024691 4.87 58.4 µM 3.21E-6 3 5.71E-7 1.03
5 SGTC_158 k038-0087 4.49 26.4 µM 1.54E-5 ubiquinone biosynthesis & proteasome 27 3.50E-6 0.10
6 SGTC_2422 chlorphenesin carbamate 4.36 384.2 µM 2.66E-5 4 6.59E-6 0.33
7 SGTC_878 0828-0289 4.30 13.7 µM 3.37E-5 18 8.66E-6 0.31
8 SGTC_837 0091-0265 4.12 12.5 µM 6.51E-5 ubiquinone biosynthesis & proteasome 11 1.86E-5 0.20
9 SGTC_1780 st038448 4.10 24.5 µM 7.13E-5 iron homeostasis 4 2.06E-5 0.02
10 SGTC_3278 9139605 4.07 49.5 µM 7.93E-5 8 2.33E-5 0.07
11 SGTC_2249 haloperidol 3.98 116.4 µM 1.10E-4 ergosterol depletion effects on membrane 41 3.41E-5 0.01
12 SGTC_142 0335-0849 3.91 62.7 µM 1.42E-4 ubiquinone biosynthesis & proteasome 19 4.56E-5 0.09
13 SGTC_444 curcumin 3.84 90.4 µM 1.84E-4 iron homeostasis 17 6.18E-5 0.08
14 SGTC_1597 7-benzyloxygramine 3.83 47.5 µM 1.93E-4 calcium & mitochondrial duress 54 6.50E-5 0.05
15 SGTC_1527 coumarin 3.81 137.0 µM 2.06E-4 7 7.02E-5 0.27
16 SGTC_1268 0828-0275 3.66 2.5 µM 3.38E-4 7 1.24E-4 0.19
17 SGTC_294 k038-0071 3.56 157.0 µM 4.81E-4 ubiquinone biosynthesis & proteasome 21 1.87E-4 0.06
18 SGTC_711 k292-0785 3.51 179.0 µM 5.62E-4 DNA intercalators 9 2.24E-4 0.20
19 SGTC_929 2805-0021 3.43 398.0 µM 7.28E-4 8 3.02E-4 0.05
20 SGTC_1151 3909-8316 3.35 15.3 µM 9.40E-4 13 4.05E-4 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.400 3.22E-129 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.390 4.45E-122 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.373 5.51E-111 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.339 1.13E-90 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.332 2.91E-87 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.328 9.38E-85 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.327 1.48E-84 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.318 1.14E-79 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.313 2.73E-77 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.285 8.59E-64 YOR362C PRE10 het Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.273 3.49E-58 YML092C PRE8 het Alpha 2 subunit of the 20S proteasome
0.207 7.46E-34 YPR103W PRE2 het Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.206 2.25E-33 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.203 1.40E-32 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.197 8.65E-31 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2