YJL011C / RPC17

RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL011C / RPC17

Click on Significant Values for Screen Details ID:SGTC_138|Compound:0850-0259|FD-Score:6.72|P-value:5.72E-10 ID:SGTC_162|Compound:k030-0015|FD-Score:-3.71|P-value:4.78E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:7.81|P-value:7.84E-13 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.43|P-value:8.67E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:3.79|P-value:2.77E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.31|P-value:4.40E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:5.97|P-value:3.04E-8 ID:SGTC_558|Compound:k064-0027|FD-Score:4.61|P-value:1.40E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:5.73|P-value:1.02E-7 ID:SGTC_839|Compound:0122-0028|FD-Score:4.17|P-value:7.25E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:-6.21|P-value:1.45E-8 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-4.14|P-value:1.12E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:5.00|P-value:2.74E-6 ID:SGTC_958|Compound:1222-0007|FD-Score:-4.42|P-value:4.17E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:3.45|P-value:8.29E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.27|P-value:5.12E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:-4.08|P-value:1.42E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.91|P-value:1.86E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:4.16|P-value:7.53E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.97|P-value:3.16E-6 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.78|P-value:2.87E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.44|P-value:8.43E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:4.19|P-value:6.84E-5 ID:SGTC_1561|Compound:5-methoxyflavone|FD-Score:-4.59|P-value:2.17E-5 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-3.71|P-value:4.80E-4 ID:SGTC_1608|Compound:st001473|FD-Score:-3.69|P-value:5.24E-4 ID:SGTC_1668|Compound:st014914|FD-Score:3.44|P-value:8.60E-4 ID:SGTC_1781|Compound:st038725|FD-Score:4.45|P-value:2.57E-5 ID:SGTC_1915|Compound:st067105|FD-Score:5.73|P-value:9.96E-8 ID:SGTC_2041|Compound:5107442|FD-Score:3.90|P-value:1.94E-4 ID:SGTC_2044|Compound:5102272|FD-Score:-5.04|P-value:3.46E-6 ID:SGTC_2147|Compound:5545932|FD-Score:3.43|P-value:8.89E-4 ID:SGTC_2155|Compound:5570423|FD-Score:3.55|P-value:5.97E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-4.56|P-value:2.37E-5 ID:SGTC_2328|Compound:6201408|FD-Score:-5.69|P-value:1.84E-7 ID:SGTC_2411|Compound:st077133|FD-Score:5.75|P-value:8.88E-8 ID:SGTC_2522|Compound:biochanin a|FD-Score:5.53|P-value:2.63E-7 ID:SGTC_2544|Compound:sphondin|FD-Score:5.11|P-value:1.75E-6 ID:SGTC_2686|Compound:alizarin|FD-Score:3.70|P-value:3.68E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.66|P-value:4.20E-4 ID:SGTC_2912|Compound:7964298|FD-Score:4.09|P-value:9.78E-5 ID:SGTC_2970|Compound:9090938|FD-Score:4.59|P-value:1.48E-5 ID:SGTC_3003|Compound:9075612|FD-Score:3.72|P-value:3.46E-4 ID:SGTC_3136|Compound:9095672|FD-Score:3.68|P-value:3.98E-4 ID:SGTC_3174|Compound:9107204|FD-Score:-3.60|P-value:6.84E-4 ID:SGTC_3252|Compound:9135871|FD-Score:4.09|P-value:9.73E-5 ID:SGTC_3293|Compound:9120939|FD-Score:4.64|P-value:1.25E-5 ID:SGTC_3300|Compound:9124615|FD-Score:-4.56|P-value:2.38E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:6.72|P-value:5.72E-10 ID:SGTC_162|Compound:k030-0015|FD-Score:-3.71|P-value:4.78E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:7.81|P-value:7.84E-13 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.43|P-value:8.67E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:3.79|P-value:2.77E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.31|P-value:4.40E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:5.97|P-value:3.04E-8 ID:SGTC_558|Compound:k064-0027|FD-Score:4.61|P-value:1.40E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:5.73|P-value:1.02E-7 ID:SGTC_839|Compound:0122-0028|FD-Score:4.17|P-value:7.25E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:-6.21|P-value:1.45E-8 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:-4.14|P-value:1.12E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:5.00|P-value:2.74E-6 ID:SGTC_958|Compound:1222-0007|FD-Score:-4.42|P-value:4.17E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:3.45|P-value:8.29E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.27|P-value:5.12E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:-4.08|P-value:1.42E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.91|P-value:1.86E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:4.16|P-value:7.53E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.97|P-value:3.16E-6 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.78|P-value:2.87E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.44|P-value:8.43E-4 ID:SGTC_1413|Compound:3910-0535|FD-Score:4.19|P-value:6.84E-5 ID:SGTC_1561|Compound:5-methoxyflavone|FD-Score:-4.59|P-value:2.17E-5 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-3.71|P-value:4.80E-4 ID:SGTC_1608|Compound:st001473|FD-Score:-3.69|P-value:5.24E-4 ID:SGTC_1668|Compound:st014914|FD-Score:3.44|P-value:8.60E-4 ID:SGTC_1781|Compound:st038725|FD-Score:4.45|P-value:2.57E-5 ID:SGTC_1915|Compound:st067105|FD-Score:5.73|P-value:9.96E-8 ID:SGTC_2041|Compound:5107442|FD-Score:3.90|P-value:1.94E-4 ID:SGTC_2044|Compound:5102272|FD-Score:-5.04|P-value:3.46E-6 ID:SGTC_2147|Compound:5545932|FD-Score:3.43|P-value:8.89E-4 ID:SGTC_2155|Compound:5570423|FD-Score:3.55|P-value:5.97E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-4.56|P-value:2.37E-5 ID:SGTC_2328|Compound:6201408|FD-Score:-5.69|P-value:1.84E-7 ID:SGTC_2411|Compound:st077133|FD-Score:5.75|P-value:8.88E-8 ID:SGTC_2522|Compound:biochanin a|FD-Score:5.53|P-value:2.63E-7 ID:SGTC_2544|Compound:sphondin|FD-Score:5.11|P-value:1.75E-6 ID:SGTC_2686|Compound:alizarin|FD-Score:3.70|P-value:3.68E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.66|P-value:4.20E-4 ID:SGTC_2912|Compound:7964298|FD-Score:4.09|P-value:9.78E-5 ID:SGTC_2970|Compound:9090938|FD-Score:4.59|P-value:1.48E-5 ID:SGTC_3003|Compound:9075612|FD-Score:3.72|P-value:3.46E-4 ID:SGTC_3136|Compound:9095672|FD-Score:3.68|P-value:3.98E-4 ID:SGTC_3174|Compound:9107204|FD-Score:-3.60|P-value:6.84E-4 ID:SGTC_3252|Compound:9135871|FD-Score:4.09|P-value:9.73E-5 ID:SGTC_3293|Compound:9120939|FD-Score:4.64|P-value:1.25E-5 ID:SGTC_3300|Compound:9124615|FD-Score:-4.56|P-value:2.38E-5

Top fitness defect scores for YJL011C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_165 k038-0023 7.81 64.4 µM 7.84E-13 ubiquinone biosynthesis & proteasome 4 2.96E-15 0.59
2 SGTC_138 0850-0259 6.72 40.9 µM 5.72E-10 cell wall 25 9.29E-12 0.07
3 SGTC_354 2783-3500 5.97 4.9 µM 3.04E-8 cell wall 13 1.18E-9 0.14
4 SGTC_2411 st077133 5.75 39.9 µM 8.88E-8 RNA pol III & RNase P/MRP 15 4.35E-9 1.12
5 SGTC_1915 st067105 5.73 30.6 µM 9.96E-8 RNA pol III & RNase P/MRP 21 5.00E-9 0.14
6 SGTC_586 0957-0265 5.73 8.2 µM 1.02E-7 cell wall 24 5.13E-9 0.00
7 SGTC_2522 biochanin a 5.53 4.9 µM 2.63E-7 RNA pol III & RNase P/MRP 7 1.63E-8 0.30
8 SGTC_2544 sphondin 5.11 68.5 µM 1.75E-6 RNA pol III & RNase P/MRP 24 1.64E-7 0.32
9 SGTC_957 1187-1561 5.00 14.1 µM 2.74E-6 3 2.81E-7 0.55
10 SGTC_1133 3473-0247 4.97 50.0 µM 3.16E-6 RSC complex & mRNA processing 33 3.34E-7 0.18
11 SGTC_3293 9120939 4.64 62.0 µM 1.25E-5 4 1.78E-6 0.01
12 SGTC_558 k064-0027 4.61 21.3 µM 1.40E-5 DNA damage response 7 2.03E-6 0.05
13 SGTC_2970 9090938 4.59 41.9 µM 1.48E-5 RNA processing & uracil transport 7 2.17E-6 0.09
14 SGTC_1781 st038725 4.45 66.3 µM 2.57E-5 NEO1 8 4.25E-6 0.08
15 SGTC_351 caspofungin 4.31 25.0 nM 4.40E-5 cell wall 27 8.15E-6 0.07
16 SGTC_994 3910-0338 4.27 138.0 µM 5.12E-5 calcium & mitochondrial duress 9 9.78E-6 0.08
17 SGTC_1413 3910-0535 4.19 34.4 µM 6.84E-5 2 1.39E-5 0.35
18 SGTC_839 0122-0028 4.17 230.0 µM 7.25E-5 RSC complex & mRNA processing 37 1.49E-5 0.19
19 SGTC_1124 fisetin 4.16 1.2 µM 7.53E-5 10 1.56E-5 0.28
20 SGTC_3252 9135871 4.09 49.5 µM 9.73E-5 5 2.13E-5 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.239 1.09E-44 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.237 4.71E-44 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.216 7.63E-37 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.211 3.74E-35 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.204 5.57E-33 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.199 1.92E-31 YDL152W_d YDL152W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.199 2.85E-31 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.193 1.50E-29 YKL144C RPC25 het RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
0.191 6.92E-29 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.184 6.03E-27 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.179 1.20E-25 YDR348C PAL1 hom Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.179 1.88E-25 YBL026W LSM2 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.178 2.42E-25 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.174 3.09E-24 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.173 4.23E-24 YFL002C SPB4 het Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients