YJL029C / VPS53

Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL029C / VPS53

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:-3.51|P-value:3.76E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:3.41|P-value:2.25E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.20|P-value:4.96E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:3.44|P-value:2.01E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.71|P-value:1.78E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.50|P-value:3.84E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.43|P-value:1.73E-10 ID:SGTC_577|Compound:r071-0003|FD-Score:3.42|P-value:2.21E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:4.45|P-value:2.83E-6 ID:SGTC_736|Compound:0958-0254|FD-Score:4.69|P-value:8.87E-7 ID:SGTC_739|Compound:1486-1183|FD-Score:-3.27|P-value:8.72E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.12|P-value:6.59E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.48|P-value:1.78E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.61|P-value:1.07E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.40|P-value:5.44E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.29|P-value:3.53E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.14|P-value:6.05E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.17|P-value:5.53E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:4.21|P-value:8.52E-6 ID:SGTC_1531|Compound:st059297|FD-Score:3.01|P-value:9.55E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.02|P-value:9.17E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.06|P-value:7.90E-4 ID:SGTC_1765|Compound:st044815|FD-Score:-6.33|P-value:3.24E-10 ID:SGTC_1793|Compound:st048256|FD-Score:-6.21|P-value:6.74E-10 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.05|P-value:8.27E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-5.43|P-value:6.37E-8 ID:SGTC_2049|Compound:5262831|FD-Score:3.57|P-value:1.26E-4 ID:SGTC_2090|Compound:5301565|FD-Score:4.19|P-value:9.22E-6 ID:SGTC_2288|Compound:7946289|FD-Score:4.15|P-value:1.11E-5 ID:SGTC_2488|Compound:5268135|FD-Score:3.53|P-value:1.43E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.53|P-value:3.46E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-5.77|P-value:9.28E-9 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.02|P-value:9.16E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.50|P-value:3.77E-4 ID:SGTC_2841|Compound:9004761|FD-Score:3.45|P-value:1.97E-4 ID:SGTC_2900|Compound:5328528|FD-Score:3.34|P-value:3.00E-4 ID:SGTC_2950|Compound:9072895|FD-Score:4.01|P-value:2.04E-5 ID:SGTC_2966|Compound:9084041|FD-Score:3.35|P-value:2.81E-4 ID:SGTC_3098|Compound:9119039|FD-Score:3.50|P-value:1.61E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-3.35|P-value:6.51E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-3.51|P-value:3.76E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:3.41|P-value:2.25E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.20|P-value:4.96E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:3.44|P-value:2.01E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.71|P-value:1.78E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.50|P-value:3.84E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.43|P-value:1.73E-10 ID:SGTC_577|Compound:r071-0003|FD-Score:3.42|P-value:2.21E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:4.45|P-value:2.83E-6 ID:SGTC_736|Compound:0958-0254|FD-Score:4.69|P-value:8.87E-7 ID:SGTC_739|Compound:1486-1183|FD-Score:-3.27|P-value:8.72E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.12|P-value:6.59E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.48|P-value:1.78E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.61|P-value:1.07E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.40|P-value:5.44E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.29|P-value:3.53E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.14|P-value:6.05E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.17|P-value:5.53E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:4.21|P-value:8.52E-6 ID:SGTC_1531|Compound:st059297|FD-Score:3.01|P-value:9.55E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.02|P-value:9.17E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.06|P-value:7.90E-4 ID:SGTC_1765|Compound:st044815|FD-Score:-6.33|P-value:3.24E-10 ID:SGTC_1793|Compound:st048256|FD-Score:-6.21|P-value:6.74E-10 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.05|P-value:8.27E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-5.43|P-value:6.37E-8 ID:SGTC_2049|Compound:5262831|FD-Score:3.57|P-value:1.26E-4 ID:SGTC_2090|Compound:5301565|FD-Score:4.19|P-value:9.22E-6 ID:SGTC_2288|Compound:7946289|FD-Score:4.15|P-value:1.11E-5 ID:SGTC_2488|Compound:5268135|FD-Score:3.53|P-value:1.43E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.53|P-value:3.46E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-5.77|P-value:9.28E-9 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.02|P-value:9.16E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.50|P-value:3.77E-4 ID:SGTC_2841|Compound:9004761|FD-Score:3.45|P-value:1.97E-4 ID:SGTC_2900|Compound:5328528|FD-Score:3.34|P-value:3.00E-4 ID:SGTC_2950|Compound:9072895|FD-Score:4.01|P-value:2.04E-5 ID:SGTC_2966|Compound:9084041|FD-Score:3.35|P-value:2.81E-4 ID:SGTC_3098|Compound:9119039|FD-Score:3.50|P-value:1.61E-4 ID:SGTC_3218|Compound:9131285|FD-Score:-3.35|P-value:6.51E-4

Top fitness defect scores for YJL029C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_736 0958-0254 4.69 92.6 µM 8.87E-7 Golgi 15 1.37E-6
2 SGTC_581 0958-0271 4.45 211.0 µM 2.83E-6 37 4.30E-6
3 SGTC_1525 8-methoxypsoralen 4.21 92.5 µM 8.52E-6 DNA damage response 23 1.28E-5
4 SGTC_2090 5301565 4.19 104.5 µM 9.22E-6 TSC3-RPN4 40 1.38E-5
5 SGTC_2288 7946289 4.15 167.3 µM 1.11E-5 22 1.66E-5
6 SGTC_2950 9072895 4.01 21.5 µM 2.04E-5 34 3.03E-5
7 SGTC_852 0418-0143 3.61 439.0 µM 1.07E-4 calcium & mitochondrial duress 87 1.54E-4
8 SGTC_2049 5262831 3.57 67.2 µM 1.26E-4 41 1.82E-4
9 SGTC_2488 5268135 3.53 45.7 µM 1.43E-4 67 2.05E-4
10 SGTC_3098 9119039 3.50 49.5 µM 1.61E-4 Golgi 49 2.30E-4
11 SGTC_841 0251-0215 3.48 91.2 µM 1.78E-4 88 2.54E-4
12 SGTC_2841 9004761 3.45 39.0 µM 1.97E-4 amide catabolism 52 2.81E-4
13 SGTC_176 1486-1328 3.44 72.8 µM 2.01E-4 81 2.87E-4
14 SGTC_577 r071-0003 3.42 38.6 µM 2.21E-4 fatty acid desaturase (OLE1) 85 3.15E-4
15 SGTC_101 0490-5701 3.41 117.5 µM 2.25E-4 31 3.20E-4
16 SGTC_2966 9084041 3.35 48.0 µM 2.81E-4 57 3.99E-4
17 SGTC_2900 5328528 3.34 85.3 µM 3.00E-4 Golgi 50 4.25E-4
18 SGTC_1386 3228-0095 3.29 33.8 µM 3.53E-4 35 4.99E-4
19 SGTC_115 0527-0192 3.20 112.0 µM 4.96E-4 124 6.97E-4
20 SGTC_1448 4255-0083 3.17 152.0 µM 5.53E-4 ERG2 46 7.74E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.58E-14 YLR382C NAM2 hom Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance
0.122 1.29E-12 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.116 1.64E-11 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.114 2.99E-11 YAL048C GEM1 hom Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases
0.104 1.61E-9 YDL075W RPL31A hom Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication
0.103 2.42E-9 YKR020W VPS51 hom Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p
0.102 3.72E-9 YJL176C SWI3 hom Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
0.101 4.58E-9 YBL071W-A KTI11 hom Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs
0.098 1.21E-8 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
0.097 1.58E-8 YMR075C-A_d YMR075C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W
0.095 3.02E-8 YNL025C SSN8 hom Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance
0.094 4.98E-8 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.093 6.82E-8 YOR008C-A_p YOR008C-A_p hom Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
0.093 7.47E-8 YJL144W YJL144W hom Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
0.092 8.18E-8 YOR106W VAM3 hom Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region