YJL033W / HCA4

DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL033W / HCA4

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:2.92|P-value:5.23E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:6.29|P-value:1.34E-12 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.29|P-value:3.68E-9 ID:SGTC_164|Compound:4100-3994|FD-Score:2.84|P-value:7.20E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:2.89|P-value:6.04E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:-3.38|P-value:1.20E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:3.76|P-value:1.31E-5 ID:SGTC_308|Compound:4092-1169|FD-Score:-3.24|P-value:2.12E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.78|P-value:1.94E-5 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:4.32|P-value:7.35E-7 ID:SGTC_904|Compound:1141-0088|FD-Score:3.52|P-value:4.23E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:2.82|P-value:7.66E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.01|P-value:3.79E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:4.92|P-value:2.08E-8 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.33|P-value:9.69E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.78|P-value:9.09E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:4.53|P-value:2.21E-7 ID:SGTC_1334|Compound:1486-0610|FD-Score:2.95|P-value:4.74E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.25|P-value:2.10E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:2.95|P-value:4.68E-4 ID:SGTC_1606|Compound:st000094|FD-Score:3.05|P-value:3.23E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-2.97|P-value:6.27E-4 ID:SGTC_1725|Compound:st036284|FD-Score:-4.05|P-value:5.05E-6 ID:SGTC_1737|Compound:st035557|FD-Score:3.18|P-value:1.86E-4 ID:SGTC_1815|Compound:st051655|FD-Score:7.97|P-value:4.72E-19 ID:SGTC_1923|Compound:st058458|FD-Score:4.16|P-value:1.70E-6 ID:SGTC_1936|Compound:st074407|FD-Score:-2.85|P-value:9.93E-4 ID:SGTC_2083|Compound:5306046|FD-Score:2.98|P-value:4.20E-4 ID:SGTC_2199|Compound:6590217|FD-Score:3.95|P-value:5.30E-6 ID:SGTC_2201|Compound:6605497|FD-Score:2.94|P-value:4.88E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.71|P-value:1.68E-5 ID:SGTC_2327|Compound:6189656|FD-Score:3.14|P-value:2.15E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-2.85|P-value:9.97E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.66|P-value:2.15E-5 ID:SGTC_2357|Compound:9067248|FD-Score:-3.81|P-value:1.69E-5 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.38|P-value:7.66E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.17|P-value:2.88E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.94|P-value:5.34E-6 ID:SGTC_2918|Compound:7977171|FD-Score:3.13|P-value:2.29E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.38|P-value:1.20E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.26|P-value:1.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-3.81|P-value:1.64E-5 ID:SGTC_3047|Compound:9092122|FD-Score:4.51|P-value:2.46E-7 ID:SGTC_3055|Compound:9094636|FD-Score:-3.20|P-value:2.54E-4 ID:SGTC_3062|Compound:9077494|FD-Score:3.07|P-value:2.91E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.26|P-value:1.31E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.35|P-value:8.90E-5 ID:SGTC_3213|Compound:9129884|FD-Score:-3.09|P-value:4.02E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-3.11|P-value:3.68E-4 ID:SGTC_3343|Compound:9148379|FD-Score:2.77|P-value:9.43E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:2.92|P-value:5.23E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:6.29|P-value:1.34E-12 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.29|P-value:3.68E-9 ID:SGTC_164|Compound:4100-3994|FD-Score:2.84|P-value:7.20E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:2.89|P-value:6.04E-4 ID:SGTC_281|Compound:1672-3440|FD-Score:-3.38|P-value:1.20E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:3.76|P-value:1.31E-5 ID:SGTC_308|Compound:4092-1169|FD-Score:-3.24|P-value:2.12E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-3.78|P-value:1.94E-5 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:4.32|P-value:7.35E-7 ID:SGTC_904|Compound:1141-0088|FD-Score:3.52|P-value:4.23E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:2.82|P-value:7.66E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.01|P-value:3.79E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:4.92|P-value:2.08E-8 ID:SGTC_1256|Compound:0570-0116|FD-Score:3.33|P-value:9.69E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.78|P-value:9.09E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:4.53|P-value:2.21E-7 ID:SGTC_1334|Compound:1486-0610|FD-Score:2.95|P-value:4.74E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.25|P-value:2.10E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:2.95|P-value:4.68E-4 ID:SGTC_1606|Compound:st000094|FD-Score:3.05|P-value:3.23E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-2.97|P-value:6.27E-4 ID:SGTC_1725|Compound:st036284|FD-Score:-4.05|P-value:5.05E-6 ID:SGTC_1737|Compound:st035557|FD-Score:3.18|P-value:1.86E-4 ID:SGTC_1815|Compound:st051655|FD-Score:7.97|P-value:4.72E-19 ID:SGTC_1923|Compound:st058458|FD-Score:4.16|P-value:1.70E-6 ID:SGTC_1936|Compound:st074407|FD-Score:-2.85|P-value:9.93E-4 ID:SGTC_2083|Compound:5306046|FD-Score:2.98|P-value:4.20E-4 ID:SGTC_2199|Compound:6590217|FD-Score:3.95|P-value:5.30E-6 ID:SGTC_2201|Compound:6605497|FD-Score:2.94|P-value:4.88E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.71|P-value:1.68E-5 ID:SGTC_2327|Compound:6189656|FD-Score:3.14|P-value:2.15E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-2.85|P-value:9.97E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.66|P-value:2.15E-5 ID:SGTC_2357|Compound:9067248|FD-Score:-3.81|P-value:1.69E-5 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.38|P-value:7.66E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.17|P-value:2.88E-4 ID:SGTC_2848|Compound:9016370|FD-Score:3.94|P-value:5.34E-6 ID:SGTC_2918|Compound:7977171|FD-Score:3.13|P-value:2.29E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.38|P-value:1.20E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.26|P-value:1.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-3.81|P-value:1.64E-5 ID:SGTC_3047|Compound:9092122|FD-Score:4.51|P-value:2.46E-7 ID:SGTC_3055|Compound:9094636|FD-Score:-3.20|P-value:2.54E-4 ID:SGTC_3062|Compound:9077494|FD-Score:3.07|P-value:2.91E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.26|P-value:1.31E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.35|P-value:8.90E-5 ID:SGTC_3213|Compound:9129884|FD-Score:-3.09|P-value:4.02E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-3.11|P-value:3.68E-4 ID:SGTC_3343|Compound:9148379|FD-Score:2.77|P-value:9.43E-4

Top fitness defect scores for YJL033W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1815 st051655 7.97 58.2 µM 4.72E-19 TSC3-RPN4 2 8.02E-16 2.00
2 SGTC_69 0096-0274 6.29 206.7 µM 1.34E-12 mitochondrial processes 20 1.56E-10 0.02
3 SGTC_1254 0550-0035 4.92 264.0 µM 2.08E-8 3 4.24E-7 0.37
4 SGTC_1276 0871-0068 4.53 94.2 µM 2.21E-7 2 2.94E-6 0.14
5 SGTC_3047 9092122 4.51 49.5 µM 2.46E-7 5 3.21E-6 0.68
6 SGTC_414 piperonyl butoxide 4.32 100.0 µM 7.35E-7 2 7.88E-6 0.31
7 SGTC_1923 st058458 4.16 19.1 µM 1.70E-6 11 1.57E-5 0.19
8 SGTC_2199 6590217 3.95 200.0 µM 5.30E-6 mitochondrial processes 3 3.98E-5 0.29
9 SGTC_2848 9016370 3.94 26.0 µM 5.34E-6 3 4.01E-5 0.09
10 SGTC_297 0986-0032 3.76 24.0 µM 1.31E-5 60S ribosome export 4 8.34E-5 0.11
11 SGTC_2324 6149169 3.71 200.0 µM 1.68E-5 7 1.03E-4 0.17
12 SGTC_2345 9052315 3.66 157.4 µM 2.15E-5 7 1.26E-4 0.15
13 SGTC_904 1141-0088 3.52 396.0 µM 4.23E-5 4 2.19E-4 0.18
14 SGTC_2392 N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide 3.38 200.0 µM 7.66E-5 11 3.56E-4 0.05
15 SGTC_3204 9114183 3.35 49.5 µM 8.90E-5 12 4.03E-4 0.01
16 SGTC_1256 0570-0116 3.33 285.0 µM 9.69E-5 10 4.32E-4 0.01
17 SGTC_3202 9113761 3.26 49.5 µM 1.31E-4 24 5.53E-4 0.12
18 SGTC_2949 9072542 3.26 2.2 µM 1.34E-4 TSC3-RPN4 22 5.64E-4 0.06
19 SGTC_1737 st035557 3.18 69.7 µM 1.86E-4 13 7.38E-4 0.05
20 SGTC_2327 6189656 3.14 200.0 µM 2.15E-4 20 8.32E-4 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 5.08E-18 YIL147C SLN1 het Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
0.140 3.25E-16 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.130 3.72E-14 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.128 9.54E-14 YGR082W TOM20 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
0.125 3.29E-13 YMR059W SEN15 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
0.124 5.20E-13 YDL120W YFH1 het Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
0.117 9.69E-12 YKR015C_p YKR015C_p hom Putative protein of unknown function
0.116 1.71E-11 YJR072C NPA3 het Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
0.115 2.90E-11 YFR031C SMC2 het Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
0.113 4.87E-11 YJR076C CDC11 het Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
0.112 7.28E-11 YOR268C_p YOR268C_p hom Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene
0.111 1.11E-10 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.107 5.90E-10 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.105 1.09E-9 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.104 1.64E-9 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes