YJL034W / KAR2

ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL034W / KAR2

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:-2.87|P-value:7.43E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-2.85|P-value:8.01E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:4.33|P-value:1.05E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:6.37|P-value:1.42E-12 ID:SGTC_277|Compound:nystatin|FD-Score:-3.06|P-value:3.59E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.39|P-value:1.05E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.26|P-value:1.78E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.20|P-value:2.14E-6 ID:SGTC_516|Compound:3992-2719|FD-Score:-5.71|P-value:1.44E-10 ID:SGTC_680|Compound:0417-1641|FD-Score:4.22|P-value:1.87E-6 ID:SGTC_758|Compound:0868-0029|FD-Score:2.85|P-value:9.07E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-2.86|P-value:7.75E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.12|P-value:3.26E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:2.97|P-value:5.84E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:2.94|P-value:6.39E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-4.08|P-value:3.22E-6 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.24|P-value:1.98E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:4.08|P-value:3.96E-6 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.05|P-value:4.27E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.26|P-value:1.84E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.21|P-value:2.21E-4 ID:SGTC_1604|Compound:st002052|FD-Score:4.08|P-value:3.93E-6 ID:SGTC_1616|Compound:st002734|FD-Score:3.66|P-value:3.06E-5 ID:SGTC_1620|Compound:st003704|FD-Score:5.97|P-value:2.80E-11 ID:SGTC_1629|Compound:st005085|FD-Score:5.37|P-value:1.97E-9 ID:SGTC_1635|Compound:st007035|FD-Score:6.29|P-value:2.50E-12 ID:SGTC_1636|Compound:st007707|FD-Score:5.37|P-value:1.98E-9 ID:SGTC_1642|Compound:st009582|FD-Score:3.01|P-value:5.05E-4 ID:SGTC_1655|Compound:st012546|FD-Score:-3.48|P-value:6.00E-5 ID:SGTC_1759|Compound:st043059|FD-Score:-3.23|P-value:1.79E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.02|P-value:4.76E-4 ID:SGTC_1812|Compound:st052057|FD-Score:3.59|P-value:4.16E-5 ID:SGTC_1849|Compound:st056239|FD-Score:3.32|P-value:1.42E-4 ID:SGTC_1853|Compound:st056710|FD-Score:-2.87|P-value:7.32E-4 ID:SGTC_1908|Compound:st060353|FD-Score:4.96|P-value:2.75E-8 ID:SGTC_1914|Compound:st062367|FD-Score:-4.26|P-value:1.28E-6 ID:SGTC_1952|Compound:st077036|FD-Score:2.85|P-value:9.28E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-2.90|P-value:6.65E-4 ID:SGTC_2360|Compound:9040895|FD-Score:2.88|P-value:8.20E-4 ID:SGTC_2365|Compound:9042475|FD-Score:-2.99|P-value:4.71E-4 ID:SGTC_2431|Compound:5490079|FD-Score:3.04|P-value:4.41E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:2.84|P-value:9.38E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.33|P-value:1.13E-4 ID:SGTC_2795|Compound:7780010|FD-Score:-3.03|P-value:4.05E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-7.62|P-value:2.23E-17 ID:SGTC_2797|Compound:7859098|FD-Score:-5.93|P-value:2.92E-11 ID:SGTC_2801|Compound:7907800|FD-Score:3.70|P-value:2.52E-5 ID:SGTC_3231|Compound:9132313|FD-Score:2.87|P-value:8.53E-4 ID:SGTC_3320|Compound:9137005|FD-Score:-3.18|P-value:2.14E-4 ID:SGTC_3324|Compound:9138759|FD-Score:3.05|P-value:4.19E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.98|P-value:6.68E-6 ID:SGTC_16|Compound:erodoxin|FD-Score:-2.87|P-value:7.43E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-2.85|P-value:8.01E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:4.33|P-value:1.05E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:6.37|P-value:1.42E-12 ID:SGTC_277|Compound:nystatin|FD-Score:-3.06|P-value:3.59E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.39|P-value:1.05E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.26|P-value:1.78E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.20|P-value:2.14E-6 ID:SGTC_516|Compound:3992-2719|FD-Score:-5.71|P-value:1.44E-10 ID:SGTC_680|Compound:0417-1641|FD-Score:4.22|P-value:1.87E-6 ID:SGTC_758|Compound:0868-0029|FD-Score:2.85|P-value:9.07E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-2.86|P-value:7.75E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.12|P-value:3.26E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:2.97|P-value:5.84E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:2.94|P-value:6.39E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-4.08|P-value:3.22E-6 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.24|P-value:1.98E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:4.08|P-value:3.96E-6 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.05|P-value:4.27E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.26|P-value:1.84E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.21|P-value:2.21E-4 ID:SGTC_1604|Compound:st002052|FD-Score:4.08|P-value:3.93E-6 ID:SGTC_1616|Compound:st002734|FD-Score:3.66|P-value:3.06E-5 ID:SGTC_1620|Compound:st003704|FD-Score:5.97|P-value:2.80E-11 ID:SGTC_1629|Compound:st005085|FD-Score:5.37|P-value:1.97E-9 ID:SGTC_1635|Compound:st007035|FD-Score:6.29|P-value:2.50E-12 ID:SGTC_1636|Compound:st007707|FD-Score:5.37|P-value:1.98E-9 ID:SGTC_1642|Compound:st009582|FD-Score:3.01|P-value:5.05E-4 ID:SGTC_1655|Compound:st012546|FD-Score:-3.48|P-value:6.00E-5 ID:SGTC_1759|Compound:st043059|FD-Score:-3.23|P-value:1.79E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.02|P-value:4.76E-4 ID:SGTC_1812|Compound:st052057|FD-Score:3.59|P-value:4.16E-5 ID:SGTC_1849|Compound:st056239|FD-Score:3.32|P-value:1.42E-4 ID:SGTC_1853|Compound:st056710|FD-Score:-2.87|P-value:7.32E-4 ID:SGTC_1908|Compound:st060353|FD-Score:4.96|P-value:2.75E-8 ID:SGTC_1914|Compound:st062367|FD-Score:-4.26|P-value:1.28E-6 ID:SGTC_1952|Compound:st077036|FD-Score:2.85|P-value:9.28E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-2.90|P-value:6.65E-4 ID:SGTC_2360|Compound:9040895|FD-Score:2.88|P-value:8.20E-4 ID:SGTC_2365|Compound:9042475|FD-Score:-2.99|P-value:4.71E-4 ID:SGTC_2431|Compound:5490079|FD-Score:3.04|P-value:4.41E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:2.84|P-value:9.38E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.33|P-value:1.13E-4 ID:SGTC_2795|Compound:7780010|FD-Score:-3.03|P-value:4.05E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-7.62|P-value:2.23E-17 ID:SGTC_2797|Compound:7859098|FD-Score:-5.93|P-value:2.92E-11 ID:SGTC_2801|Compound:7907800|FD-Score:3.70|P-value:2.52E-5 ID:SGTC_3231|Compound:9132313|FD-Score:2.87|P-value:8.53E-4 ID:SGTC_3320|Compound:9137005|FD-Score:-3.18|P-value:2.14E-4 ID:SGTC_3324|Compound:9138759|FD-Score:3.05|P-value:4.19E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.98|P-value:6.68E-6

Top fitness defect scores for YJL034W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_69 0096-0274 6.37 206.7 µM 1.42E-12 mitochondrial processes 19 9.68E-11 0.07
2 SGTC_1635 st007035 6.29 42.4 µM 2.50E-12 4 1.54E-10 0.50
3 SGTC_1620 st003704 5.97 16.5 µM 2.80E-11 1 1.15E-9 0.92
4 SGTC_1629 st005085 5.37 44.9 µM 1.97E-9 RPP1 & pyrimidine depletion 6 3.98E-8 0.02
5 SGTC_1636 st007707 5.37 68.9 µM 1.98E-9 5 3.99E-8 1.01
6 SGTC_1908 st060353 4.96 56.5 µM 2.75E-8 RPP1 & pyrimidine depletion 5 3.58E-7 0.07
7 SGTC_67 0335-0847 4.33 266.0 µM 1.05E-6 ubiquinone biosynthesis & proteasome 16 7.51E-6 0.14
8 SGTC_680 0417-1641 4.22 114.0 µM 1.87E-6 RPP1 & pyrimidine depletion 6 1.21E-5 0.37
9 SGTC_514 4466-0038 4.20 2.6 nM 2.14E-6 cell wall 34 1.36E-5 0.02
10 SGTC_1604 st002052 4.08 72.4 µM 3.93E-6 TSC3-RPN4 7 2.26E-5 0.09
11 SGTC_1286 0929-0070 4.08 131.0 µM 3.96E-6 5 2.27E-5 0.39
12 SGTC_3346 9158254 3.98 43.6 µM 6.68E-6 12 3.52E-5 0.01
13 SGTC_2801 7907800 3.70 81.8 µM 2.52E-5 7 1.07E-4 0.04
14 SGTC_1616 st002734 3.66 56.1 µM 3.06E-5 8 1.26E-4 0.03
15 SGTC_1812 st052057 3.59 4.3 µM 4.16E-5 calcium & mitochondrial duress 11 1.63E-4 0.03
16 SGTC_474 dl-dihydrosphingosine 3.39 5.0 µM 1.05E-4 14 3.55E-4 0.18
17 SGTC_1849 st056239 3.32 19.2 µM 1.42E-4 RPP1 & pyrimidine depletion 9 4.57E-4 0.21
18 SGTC_513 4245-1575 3.26 41.7 µM 1.78E-4 unfolded protein response 49 5.51E-4 0.04
19 SGTC_1506 4226-2149 3.26 212.0 µM 1.84E-4 11 5.66E-4 0.17
20 SGTC_1284 0929-0043 3.24 16.0 µM 1.98E-4 11 6.03E-4 0.14

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 3.10E-33 YNR018W RCF2 hom Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
0.186 1.68E-27 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.170 3.24E-23 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.168 1.38E-22 YGL191W COX13 hom Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.157 7.67E-20 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.155 1.32E-19 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.148 5.16E-18 YOR293C-A_p YOR293C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.146 2.09E-17 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.141 2.18E-16 YMR253C_p YMR253C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
0.141 2.66E-16 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.139 5.82E-16 YKL155C RSM22 hom Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.134 8.29E-15 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.131 3.20E-14 YJL180C ATP12 hom Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
0.127 1.94E-13 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.126 2.83E-13 YNR045W PET494 hom Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane