HIPHOP chemogenomics database

YJL034W / KAR2

ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p

Zygosity: Heterozygous strain

fixedexpanded
Profile for YJL034W / KAR2

Click on Significant Values for Screen Details

Top fitness defect scores for YJL034W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_69	0096-0274	6.37	206.7 µM	1.42E-12	mitochondrial processes	19	9.68E-11	0.07
2	SGTC_1635	st007035	6.29	42.4 µM	2.50E-12		4	1.54E-10	0.50
3	SGTC_1620	st003704	5.97	16.5 µM	2.80E-11		1	1.15E-9	0.92
4	SGTC_1629	st005085	5.37	44.9 µM	1.97E-9	RPP1 & pyrimidine depletion	6	3.98E-8	0.02
5	SGTC_1636	st007707	5.37	68.9 µM	1.98E-9		5	3.99E-8	1.01
6	SGTC_1908	st060353	4.96	56.5 µM	2.75E-8	RPP1 & pyrimidine depletion	5	3.58E-7	0.07
7	SGTC_67	0335-0847	4.33	266.0 µM	1.05E-6	ubiquinone biosynthesis & proteasome	16	7.51E-6	0.14
8	SGTC_680	0417-1641	4.22	114.0 µM	1.87E-6	RPP1 & pyrimidine depletion	6	1.21E-5	0.37
9	SGTC_514	4466-0038	4.20	2.6 nM	2.14E-6	cell wall	34	1.36E-5	0.02
10	SGTC_1604	st002052	4.08	72.4 µM	3.93E-6	TSC3-RPN4	7	2.26E-5	0.09
11	SGTC_1286	0929-0070	4.08	131.0 µM	3.96E-6		5	2.27E-5	0.39
12	SGTC_3346	9158254	3.98	43.6 µM	6.68E-6		12	3.52E-5	0.01
13	SGTC_2801	7907800	3.70	81.8 µM	2.52E-5		7	1.07E-4	0.04
14	SGTC_1616	st002734	3.66	56.1 µM	3.06E-5		8	1.26E-4	0.03
15	SGTC_1812	st052057	3.59	4.3 µM	4.16E-5	calcium & mitochondrial duress	11	1.63E-4	0.03
16	SGTC_474	dl-dihydrosphingosine	3.39	5.0 µM	1.05E-4		14	3.55E-4	0.18
17	SGTC_1849	st056239	3.32	19.2 µM	1.42E-4	RPP1 & pyrimidine depletion	9	4.57E-4	0.21
18	SGTC_513	4245-1575	3.26	41.7 µM	1.78E-4	unfolded protein response	49	5.51E-4	0.04
19	SGTC_1506	4226-2149	3.26	212.0 µM	1.84E-4		11	5.66E-4	0.17
20	SGTC_1284	0929-0043	3.24	16.0 µM	1.98E-4		11	6.03E-4	0.14

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.205	3.10E-33	YNR018W	RCF2	hom	Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
0.186	1.68E-27	YMR151W_d	YIM2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.170	3.24E-23	YLL009C	COX17	hom	Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.168	1.38E-22	YGL191W	COX13	hom	Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.157	7.67E-20	YGR183C	QCR9	hom	Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.155	1.32E-19	YEL059C-A	SOM1	hom	Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.148	5.16E-18	YOR293C-A_p	YOR293C-A_p	hom	Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.146	2.09E-17	YNR042W_d	YNR042W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.141	2.18E-16	YMR253C_p	YMR253C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
0.141	2.66E-16	YPL243W	SRP68	het	Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.139	5.82E-16	YKL155C	RSM22	hom	Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.134	8.29E-15	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.131	3.20E-14	YJL180C	ATP12	hom	Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
0.127	1.94E-13	YGL119W	COQ8	hom	Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.126	2.83E-13	YNR045W	PET494	hom	Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane