YJL050W / MTR4

ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL050W / MTR4

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.64|P-value:1.18E-4 ID:SGTC_19|Compound:0279-0214|FD-Score:-3.42|P-value:3.73E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-4.33|P-value:9.19E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.68|P-value:1.40E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:3.82|P-value:5.81E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:-3.42|P-value:3.67E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.40|P-value:3.00E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:3.27|P-value:4.81E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.98|P-value:2.91E-5 ID:SGTC_817|Compound:1391-0715|FD-Score:-4.78|P-value:1.11E-6 ID:SGTC_944|Compound:3370-0224|FD-Score:3.16|P-value:6.88E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-4.26|P-value:1.26E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.80|P-value:6.27E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.56|P-value:1.64E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.85|P-value:5.07E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.73|P-value:8.22E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.17|P-value:6.69E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:5.09|P-value:1.52E-7 ID:SGTC_1606|Compound:st000094|FD-Score:4.57|P-value:2.03E-6 ID:SGTC_1625|Compound:st003711|FD-Score:3.27|P-value:4.71E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.80|P-value:8.75E-5 ID:SGTC_1632|Compound:st005650|FD-Score:4.24|P-value:9.46E-6 ID:SGTC_1648|Compound:st011736|FD-Score:3.50|P-value:2.01E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.57|P-value:1.53E-4 ID:SGTC_2055|Compound:5235184|FD-Score:3.26|P-value:4.95E-4 ID:SGTC_2106|Compound:5357045|FD-Score:3.10|P-value:8.46E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-14.80|P-value:3.28E-49 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.22|P-value:7.49E-4 ID:SGTC_2449|Compound:5812982|FD-Score:3.13|P-value:7.81E-4 ID:SGTC_2483|Compound:5729050|FD-Score:6.00|P-value:8.05E-10 ID:SGTC_2490|Compound:carminic acid|FD-Score:4.16|P-value:1.38E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.79|P-value:6.95E-7 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.63|P-value:1.67E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-4.68|P-value:1.78E-6 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.17|P-value:6.63E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.77|P-value:6.96E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:7.11|P-value:4.61E-13 ID:SGTC_2726|Compound:cimetidine|FD-Score:-3.54|P-value:2.42E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.28|P-value:6.03E-4 ID:SGTC_2794|Compound:7785382|FD-Score:6.03|P-value:6.63E-10 ID:SGTC_2889|Compound:9059656|FD-Score:3.08|P-value:9.12E-4 ID:SGTC_2964|Compound:9083345|FD-Score:-4.11|P-value:2.44E-5 ID:SGTC_2971|Compound:9092186|FD-Score:3.68|P-value:9.97E-5 ID:SGTC_2972|Compound:9093619|FD-Score:6.25|P-value:1.66E-10 ID:SGTC_3002|Compound:9075603|FD-Score:-3.17|P-value:8.93E-4 ID:SGTC_3054|Compound:9094397|FD-Score:-3.52|P-value:2.54E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.91|P-value:4.00E-5 ID:SGTC_3156|Compound:9099931|FD-Score:3.75|P-value:7.53E-5 ID:SGTC_3192|Compound:9110362|FD-Score:4.14|P-value:1.51E-5 ID:SGTC_3231|Compound:9132313|FD-Score:-3.17|P-value:8.79E-4 ID:SGTC_3236|Compound:9132701|FD-Score:-5.01|P-value:3.49E-7 ID:SGTC_3242|Compound:9133940|FD-Score:3.53|P-value:1.82E-4 ID:SGTC_3246|Compound:9135460|FD-Score:-3.32|P-value:5.36E-4 ID:SGTC_3276|Compound:9139060|FD-Score:3.63|P-value:1.24E-4 ID:SGTC_3302|Compound:9125395|FD-Score:3.09|P-value:8.97E-4 ID:SGTC_3315|Compound:9136395|FD-Score:4.11|P-value:1.68E-5 ID:SGTC_6|Compound:0844-0013|FD-Score:3.64|P-value:1.18E-4 ID:SGTC_19|Compound:0279-0214|FD-Score:-3.42|P-value:3.73E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:-4.33|P-value:9.19E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.68|P-value:1.40E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:3.82|P-value:5.81E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:-3.42|P-value:3.67E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.40|P-value:3.00E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:3.27|P-value:4.81E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:3.98|P-value:2.91E-5 ID:SGTC_817|Compound:1391-0715|FD-Score:-4.78|P-value:1.11E-6 ID:SGTC_944|Compound:3370-0224|FD-Score:3.16|P-value:6.88E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-4.26|P-value:1.26E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.80|P-value:6.27E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:3.56|P-value:1.64E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.85|P-value:5.07E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.73|P-value:8.22E-5 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.17|P-value:6.69E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:5.09|P-value:1.52E-7 ID:SGTC_1606|Compound:st000094|FD-Score:4.57|P-value:2.03E-6 ID:SGTC_1625|Compound:st003711|FD-Score:3.27|P-value:4.71E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.80|P-value:8.75E-5 ID:SGTC_1632|Compound:st005650|FD-Score:4.24|P-value:9.46E-6 ID:SGTC_1648|Compound:st011736|FD-Score:3.50|P-value:2.01E-4 ID:SGTC_2049|Compound:5262831|FD-Score:3.57|P-value:1.53E-4 ID:SGTC_2055|Compound:5235184|FD-Score:3.26|P-value:4.95E-4 ID:SGTC_2106|Compound:5357045|FD-Score:3.10|P-value:8.46E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-14.80|P-value:3.28E-49 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.22|P-value:7.49E-4 ID:SGTC_2449|Compound:5812982|FD-Score:3.13|P-value:7.81E-4 ID:SGTC_2483|Compound:5729050|FD-Score:6.00|P-value:8.05E-10 ID:SGTC_2490|Compound:carminic acid|FD-Score:4.16|P-value:1.38E-5 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.79|P-value:6.95E-7 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.63|P-value:1.67E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-4.68|P-value:1.78E-6 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.17|P-value:6.63E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.77|P-value:6.96E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:7.11|P-value:4.61E-13 ID:SGTC_2726|Compound:cimetidine|FD-Score:-3.54|P-value:2.42E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.28|P-value:6.03E-4 ID:SGTC_2794|Compound:7785382|FD-Score:6.03|P-value:6.63E-10 ID:SGTC_2889|Compound:9059656|FD-Score:3.08|P-value:9.12E-4 ID:SGTC_2964|Compound:9083345|FD-Score:-4.11|P-value:2.44E-5 ID:SGTC_2971|Compound:9092186|FD-Score:3.68|P-value:9.97E-5 ID:SGTC_2972|Compound:9093619|FD-Score:6.25|P-value:1.66E-10 ID:SGTC_3002|Compound:9075603|FD-Score:-3.17|P-value:8.93E-4 ID:SGTC_3054|Compound:9094397|FD-Score:-3.52|P-value:2.54E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.91|P-value:4.00E-5 ID:SGTC_3156|Compound:9099931|FD-Score:3.75|P-value:7.53E-5 ID:SGTC_3192|Compound:9110362|FD-Score:4.14|P-value:1.51E-5 ID:SGTC_3231|Compound:9132313|FD-Score:-3.17|P-value:8.79E-4 ID:SGTC_3236|Compound:9132701|FD-Score:-5.01|P-value:3.49E-7 ID:SGTC_3242|Compound:9133940|FD-Score:3.53|P-value:1.82E-4 ID:SGTC_3246|Compound:9135460|FD-Score:-3.32|P-value:5.36E-4 ID:SGTC_3276|Compound:9139060|FD-Score:3.63|P-value:1.24E-4 ID:SGTC_3302|Compound:9125395|FD-Score:3.09|P-value:8.97E-4 ID:SGTC_3315|Compound:9136395|FD-Score:4.11|P-value:1.68E-5

Top fitness defect scores for YJL050W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2690 usnic acid 7.11 100.0 µM 4.61E-13 1 5.76E-13 2.09
2 SGTC_2972 9093619 6.25 59.9 µM 1.66E-10 1 2.04E-10 1.03
3 SGTC_2794 7785382 6.03 27.3 µM 6.63E-10 4 8.11E-10 0.29
4 SGTC_2483 5729050 6.00 27.2 µM 8.05E-10 2 9.83E-10 1.33
5 SGTC_1496 4469-0713 5.09 57.4 µM 1.52E-7 2 1.81E-7 0.85
6 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.79 3.7 µM 6.95E-7 6 8.25E-7 0.01
7 SGTC_1606 st000094 4.57 49.2 µM 2.03E-6 60S ribosome export 3 2.40E-6 0.36
8 SGTC_1632 st005650 4.24 90.0 µM 9.46E-6 4 1.11E-5 0.08
9 SGTC_2490 carminic acid 4.16 95.4 µM 1.38E-5 7 1.61E-5 0.46
10 SGTC_3192 9110362 4.14 49.5 µM 1.51E-5 mitochondrial processes 4 1.76E-5 0.17
11 SGTC_3315 9136395 4.11 68.5 µM 1.68E-5 6 1.95E-5 0.04
12 SGTC_610 4215-0184 3.98 155.0 µM 2.91E-5 exosome 24 3.38E-5 0.02
13 SGTC_3147 9097027 3.91 49.5 µM 4.00E-5 6 4.63E-5 0.01
14 SGTC_1177 0983-0204 3.85 23.6 µM 5.07E-5 11 5.87E-5 0.14
15 SGTC_369 0957-0393 3.82 4.5 µM 5.81E-5 cell wall signaling 10 6.72E-5 0.02
16 SGTC_1059 3448-6867 3.80 171.0 µM 6.27E-5 7 7.24E-5 0.25
17 SGTC_2592 piperlongumine 3.77 83.3 µM 6.96E-5 TSC3-RPN4 7 8.03E-5 0.15
18 SGTC_3156 9099931 3.75 49.5 µM 7.53E-5 2 8.69E-5 0.12
19 SGTC_1262 0737-0114 3.73 49.6 µM 8.22E-5 3 9.48E-5 0.07
20 SGTC_2971 9092186 3.68 9.9 µM 9.97E-5 ergosterol biosynthesis 11 1.15E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.185 2.88E-27 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.168 1.27E-22 YGR123C PPT1 hom Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
0.165 7.63E-22 YBR165W UBS1 hom Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
0.162 4.45E-21 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.160 1.09E-20 YDR119W_p VBA4_p hom Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
0.149 4.82E-18 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.147 1.17E-17 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.134 8.15E-15 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.128 8.23E-14 YDL027C_p YDL027C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
0.124 4.51E-13 YBR199W KTR4 hom Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family
0.122 1.44E-12 YMR006C PLB2 hom Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
0.122 1.57E-12 YKL044W_p YKL044W_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data
0.121 1.86E-12 YOL162W_p YOL162W_p hom Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family
0.119 4.27E-12 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.118 6.11E-12 YAL045C_d YAL045C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A