YJL062W / LAS21

Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL062W / LAS21

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:4.43|P-value:6.20E-6 ID:SGTC_44|Compound:0929-0055|FD-Score:3.77|P-value:1.02E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.72|P-value:1.22E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.10|P-value:2.70E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:4.39|P-value:7.55E-6 ID:SGTC_229|Compound:benomyl|FD-Score:-3.15|P-value:9.01E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.56|P-value:2.25E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:3.75|P-value:1.10E-4 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.61|P-value:1.90E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:4.47|P-value:5.28E-6 ID:SGTC_730|Compound:1436-0052|FD-Score:4.83|P-value:9.35E-7 ID:SGTC_744|Compound:1594-0068|FD-Score:3.67|P-value:1.49E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:5.30|P-value:8.50E-8 ID:SGTC_797|Compound:k781-0471|FD-Score:3.90|P-value:6.05E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:6.85|P-value:6.95E-12 ID:SGTC_807|Compound:1315-0151|FD-Score:3.14|P-value:9.81E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:4.56|P-value:3.45E-6 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.97|P-value:4.60E-5 ID:SGTC_928|Compound:2764-0428|FD-Score:5.37|P-value:5.87E-8 ID:SGTC_969|Compound:1313-0238|FD-Score:4.05|P-value:3.22E-5 ID:SGTC_976|Compound:1348-1067|FD-Score:6.76|P-value:1.29E-11 ID:SGTC_1076|Compound:itraconazole|FD-Score:5.93|P-value:2.45E-9 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.33|P-value:5.16E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.45|P-value:3.21E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:3.53|P-value:2.56E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.96|P-value:4.70E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.29|P-value:5.97E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.83|P-value:9.38E-7 ID:SGTC_1400|Compound:3771-8998|FD-Score:4.02|P-value:3.65E-5 ID:SGTC_1411|Compound:3909-8223|FD-Score:3.53|P-value:2.53E-4 ID:SGTC_1521|Compound:st012942|FD-Score:3.73|P-value:1.19E-4 ID:SGTC_1594|Compound:piperine|FD-Score:4.73|P-value:1.56E-6 ID:SGTC_1613|Compound:st002381|FD-Score:4.38|P-value:7.75E-6 ID:SGTC_1714|Compound:st033236|FD-Score:3.34|P-value:5.02E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-4.54|P-value:3.42E-6 ID:SGTC_1859|Compound:st055998|FD-Score:-3.94|P-value:4.84E-5 ID:SGTC_1862|Compound:st057513|FD-Score:4.94|P-value:5.49E-7 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.76|P-value:1.04E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.25|P-value:6.80E-4 ID:SGTC_1921|Compound:5740136|FD-Score:7.50|P-value:6.64E-14 ID:SGTC_1927|Compound:st060202|FD-Score:-4.19|P-value:1.70E-5 ID:SGTC_1938|Compound:st074717|FD-Score:3.30|P-value:5.76E-4 ID:SGTC_1991|Compound:st073000|FD-Score:6.16|P-value:5.99E-10 ID:SGTC_2001|Compound:4023502|FD-Score:-4.06|P-value:2.91E-5 ID:SGTC_2005|Compound:4024620|FD-Score:4.22|P-value:1.60E-5 ID:SGTC_2115|Compound:tiliquinol|FD-Score:3.97|P-value:4.62E-5 ID:SGTC_2148|Compound:5546263|FD-Score:3.24|P-value:6.98E-4 ID:SGTC_2193|Compound:5925765|FD-Score:3.90|P-value:5.97E-5 ID:SGTC_2268|Compound:7933382|FD-Score:3.15|P-value:9.39E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.39|P-value:4.17E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:3.45|P-value:3.39E-4 ID:SGTC_2585|Compound:alliin|FD-Score:3.21|P-value:7.74E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:4.56|P-value:3.40E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.75|P-value:1.10E-4 ID:SGTC_2706|Compound:st078239|FD-Score:3.15|P-value:9.59E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.22|P-value:7.65E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-4.29|P-value:1.09E-5 ID:SGTC_2822|Compound:7994718|FD-Score:-3.55|P-value:2.22E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.41|P-value:3.95E-4 ID:SGTC_2885|Compound:9057732|FD-Score:3.84|P-value:7.82E-5 ID:SGTC_3016|Compound:9080866|FD-Score:3.88|P-value:6.46E-5 ID:SGTC_3031|Compound:9088762|FD-Score:3.91|P-value:5.83E-5 ID:SGTC_3084|Compound:9116877|FD-Score:8.70|P-value:4.39E-18 ID:SGTC_3089|Compound:9117425|FD-Score:3.47|P-value:3.18E-4 ID:SGTC_3091|Compound:9116372|FD-Score:5.76|P-value:6.48E-9 ID:SGTC_3129|Compound:9127257|FD-Score:4.15|P-value:2.10E-5 ID:SGTC_3131|Compound:9127911|FD-Score:4.36|P-value:8.68E-6 ID:SGTC_3199|Compound:9111318|FD-Score:3.34|P-value:4.99E-4 ID:SGTC_3209|Compound:9113616|FD-Score:-3.19|P-value:8.01E-4 ID:SGTC_3263|Compound:9137018|FD-Score:3.69|P-value:1.35E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.51|P-value:2.75E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.30|P-value:5.39E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:4.43|P-value:6.20E-6 ID:SGTC_44|Compound:0929-0055|FD-Score:3.77|P-value:1.02E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.72|P-value:1.22E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.10|P-value:2.70E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:4.39|P-value:7.55E-6 ID:SGTC_229|Compound:benomyl|FD-Score:-3.15|P-value:9.01E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.56|P-value:2.25E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:3.75|P-value:1.10E-4 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.61|P-value:1.90E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:4.47|P-value:5.28E-6 ID:SGTC_730|Compound:1436-0052|FD-Score:4.83|P-value:9.35E-7 ID:SGTC_744|Compound:1594-0068|FD-Score:3.67|P-value:1.49E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:5.30|P-value:8.50E-8 ID:SGTC_797|Compound:k781-0471|FD-Score:3.90|P-value:6.05E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:6.85|P-value:6.95E-12 ID:SGTC_807|Compound:1315-0151|FD-Score:3.14|P-value:9.81E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:4.56|P-value:3.45E-6 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:3.97|P-value:4.60E-5 ID:SGTC_928|Compound:2764-0428|FD-Score:5.37|P-value:5.87E-8 ID:SGTC_969|Compound:1313-0238|FD-Score:4.05|P-value:3.22E-5 ID:SGTC_976|Compound:1348-1067|FD-Score:6.76|P-value:1.29E-11 ID:SGTC_1076|Compound:itraconazole|FD-Score:5.93|P-value:2.45E-9 ID:SGTC_1238|Compound:0333-0287|FD-Score:3.33|P-value:5.16E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.45|P-value:3.21E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:3.53|P-value:2.56E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.96|P-value:4.70E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.29|P-value:5.97E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.83|P-value:9.38E-7 ID:SGTC_1400|Compound:3771-8998|FD-Score:4.02|P-value:3.65E-5 ID:SGTC_1411|Compound:3909-8223|FD-Score:3.53|P-value:2.53E-4 ID:SGTC_1521|Compound:st012942|FD-Score:3.73|P-value:1.19E-4 ID:SGTC_1594|Compound:piperine|FD-Score:4.73|P-value:1.56E-6 ID:SGTC_1613|Compound:st002381|FD-Score:4.38|P-value:7.75E-6 ID:SGTC_1714|Compound:st033236|FD-Score:3.34|P-value:5.02E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-4.54|P-value:3.42E-6 ID:SGTC_1859|Compound:st055998|FD-Score:-3.94|P-value:4.84E-5 ID:SGTC_1862|Compound:st057513|FD-Score:4.94|P-value:5.49E-7 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.76|P-value:1.04E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.25|P-value:6.80E-4 ID:SGTC_1921|Compound:5740136|FD-Score:7.50|P-value:6.64E-14 ID:SGTC_1927|Compound:st060202|FD-Score:-4.19|P-value:1.70E-5 ID:SGTC_1938|Compound:st074717|FD-Score:3.30|P-value:5.76E-4 ID:SGTC_1991|Compound:st073000|FD-Score:6.16|P-value:5.99E-10 ID:SGTC_2001|Compound:4023502|FD-Score:-4.06|P-value:2.91E-5 ID:SGTC_2005|Compound:4024620|FD-Score:4.22|P-value:1.60E-5 ID:SGTC_2115|Compound:tiliquinol|FD-Score:3.97|P-value:4.62E-5 ID:SGTC_2148|Compound:5546263|FD-Score:3.24|P-value:6.98E-4 ID:SGTC_2193|Compound:5925765|FD-Score:3.90|P-value:5.97E-5 ID:SGTC_2268|Compound:7933382|FD-Score:3.15|P-value:9.39E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.39|P-value:4.17E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:3.45|P-value:3.39E-4 ID:SGTC_2585|Compound:alliin|FD-Score:3.21|P-value:7.74E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:4.56|P-value:3.40E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.75|P-value:1.10E-4 ID:SGTC_2706|Compound:st078239|FD-Score:3.15|P-value:9.59E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.22|P-value:7.65E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-4.29|P-value:1.09E-5 ID:SGTC_2822|Compound:7994718|FD-Score:-3.55|P-value:2.22E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.41|P-value:3.95E-4 ID:SGTC_2885|Compound:9057732|FD-Score:3.84|P-value:7.82E-5 ID:SGTC_3016|Compound:9080866|FD-Score:3.88|P-value:6.46E-5 ID:SGTC_3031|Compound:9088762|FD-Score:3.91|P-value:5.83E-5 ID:SGTC_3084|Compound:9116877|FD-Score:8.70|P-value:4.39E-18 ID:SGTC_3089|Compound:9117425|FD-Score:3.47|P-value:3.18E-4 ID:SGTC_3091|Compound:9116372|FD-Score:5.76|P-value:6.48E-9 ID:SGTC_3129|Compound:9127257|FD-Score:4.15|P-value:2.10E-5 ID:SGTC_3131|Compound:9127911|FD-Score:4.36|P-value:8.68E-6 ID:SGTC_3199|Compound:9111318|FD-Score:3.34|P-value:4.99E-4 ID:SGTC_3209|Compound:9113616|FD-Score:-3.19|P-value:8.01E-4 ID:SGTC_3263|Compound:9137018|FD-Score:3.69|P-value:1.35E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.51|P-value:2.75E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.30|P-value:5.39E-4

Top fitness defect scores for YJL062W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3084 9116877 8.70 49.5 µM 4.39E-18 1 1.64E-18
2 SGTC_1921 5740136 7.50 50.0 µM 6.64E-14 TRP & mitochondrial translation 7 3.16E-14
3 SGTC_800 3448-7362 6.85 155.0 µM 6.95E-12 translation 28 3.71E-12
4 SGTC_976 1348-1067 6.76 47.6 µM 1.29E-11 60S ribosome export 3 6.99E-12
5 SGTC_1991 st073000 6.16 8.9 µM 5.99E-10 NEO1 32 3.58E-10
6 SGTC_1076 itraconazole 5.93 331.0 nM 2.45E-9 8 1.52E-9
7 SGTC_3091 9116372 5.76 49.5 µM 6.48E-9 3 4.11E-9
8 SGTC_928 2764-0428 5.37 58.7 µM 5.87E-8 cell wall 23 3.93E-8
9 SGTC_767 0929-0023 5.30 86.0 µM 8.50E-8 TRP & mitochondrial translation 25 5.75E-8
10 SGTC_1862 st057513 4.94 3.8 µM 5.49E-7 2 3.89E-7
11 SGTC_730 1436-0052 4.83 41.2 µM 9.35E-7 mitochondrial processes 3 6.72E-7
12 SGTC_1386 3228-0095 4.83 33.8 µM 9.38E-7 7 6.74E-7
13 SGTC_1594 piperine 4.73 70.1 µM 1.56E-6 5 1.14E-6
14 SGTC_2600 tangeritin 4.56 88.0 µM 3.40E-6 20 2.52E-6
15 SGTC_808 0711-0032 4.56 135.0 µM 3.45E-6 5 2.56E-6
16 SGTC_723 4182-0001 4.47 115.0 µM 5.28E-6 19 3.96E-6
17 SGTC_43 0570-0105 4.43 72.5 µM 6.20E-6 ERG2 76 4.67E-6
18 SGTC_227 fluconazole 4.39 33.5 µM 7.55E-6 azole & statin 68 5.72E-6
19 SGTC_1613 st002381 4.38 52.1 µM 7.75E-6 15 5.87E-6
20 SGTC_3131 9127911 4.36 49.5 µM 8.68E-6 18 6.59E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.105 1.02E-9 YBR238C YBR238C hom Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
0.100 5.65E-9 YGL008C PMA1 het Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
0.092 8.92E-8 YJR145C RPS4A hom Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
0.088 3.52E-7 YGL007W_d BRP1_d hom Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
0.084 1.07E-6 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.082 1.97E-6 YGR118W RPS23A hom Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
0.082 2.16E-6 YML128C MSC1 hom Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated
0.078 6.34E-6 YDR525W_d API2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
0.078 6.43E-6 YEL065W SIT1 hom Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
0.078 6.56E-6 YJL069C UTP18 het Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
0.075 1.21E-5 YOR006C TSR3 hom Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.074 1.67E-5 YGL012W ERG4 hom C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
0.073 2.60E-5 YNR063W_p YNR063W_p hom Putative zinc-cluster protein of unknown function
0.072 3.21E-5 YNL100W AIM37 hom Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.071 3.94E-5 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33