YJL073W / JEM1

DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL073W / JEM1

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:-3.32|P-value:6.07E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.34|P-value:1.20E-5 ID:SGTC_17|Compound:0960-0276|FD-Score:3.79|P-value:1.11E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.21|P-value:2.08E-5 ID:SGTC_92|Compound:0063-0024|FD-Score:3.65|P-value:1.90E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:17.10|P-value:1.61E-62 ID:SGTC_223|Compound:busulfan|FD-Score:3.44|P-value:4.02E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.58|P-value:2.42E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.68|P-value:1.68E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:-4.50|P-value:5.74E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.51|P-value:5.59E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:6.86|P-value:1.21E-11 ID:SGTC_342|Compound:k048-0088|FD-Score:3.55|P-value:2.72E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.29|P-value:6.85E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:8.35|P-value:2.14E-16 ID:SGTC_662|Compound:k832-1615|FD-Score:3.67|P-value:1.74E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:4.88|P-value:9.97E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:8.17|P-value:8.47E-16 ID:SGTC_738|Compound:1315-0003|FD-Score:-4.50|P-value:5.80E-6 ID:SGTC_829|Compound:1635-0120|FD-Score:3.25|P-value:7.93E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:5.06|P-value:4.21E-7 ID:SGTC_883|Compound:0850-0245|FD-Score:4.83|P-value:1.30E-6 ID:SGTC_951|Compound:1171-0579|FD-Score:8.24|P-value:5.17E-16 ID:SGTC_970|Compound:1315-0089|FD-Score:4.11|P-value:3.11E-5 ID:SGTC_977|Compound:1348-1378|FD-Score:4.32|P-value:1.29E-5 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.12|P-value:3.05E-5 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.23|P-value:8.29E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:4.32|P-value:1.29E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.28|P-value:6.79E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:5.12|P-value:3.10E-7 ID:SGTC_1209|Compound:2323-2347|FD-Score:4.39|P-value:9.53E-6 ID:SGTC_1215|Compound:3852-0577|FD-Score:5.00|P-value:5.64E-7 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.52|P-value:3.09E-4 ID:SGTC_1605|Compound:st000082|FD-Score:-3.66|P-value:1.76E-4 ID:SGTC_1606|Compound:st000094|FD-Score:4.67|P-value:2.72E-6 ID:SGTC_1621|Compound:st003705|FD-Score:3.65|P-value:1.89E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:-3.22|P-value:8.39E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:3.21|P-value:8.84E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.38|P-value:4.99E-4 ID:SGTC_1950|Compound:st077106|FD-Score:3.66|P-value:1.83E-4 ID:SGTC_2049|Compound:5262831|FD-Score:6.55|P-value:8.66E-11 ID:SGTC_2054|Compound:5233173|FD-Score:3.51|P-value:3.18E-4 ID:SGTC_2060|Compound:5238662|FD-Score:-3.40|P-value:4.47E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.49|P-value:3.45E-4 ID:SGTC_2268|Compound:7933382|FD-Score:3.58|P-value:2.48E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.20|P-value:9.17E-4 ID:SGTC_2315|Compound:7733312|FD-Score:4.36|P-value:1.12E-5 ID:SGTC_2412|Compound:st077135|FD-Score:-4.88|P-value:9.60E-7 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.91|P-value:7.01E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:-3.44|P-value:3.92E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.46|P-value:3.77E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:9.86|P-value:3.95E-22 ID:SGTC_2711|Compound:st079103|FD-Score:3.53|P-value:2.98E-4 ID:SGTC_2837|Compound:9008476|FD-Score:4.42|P-value:8.25E-6 ID:SGTC_2840|Compound:9008771|FD-Score:-3.86|P-value:8.22E-5 ID:SGTC_2844|Compound:9010103|FD-Score:5.06|P-value:4.28E-7 ID:SGTC_2845|Compound:9003024|FD-Score:5.06|P-value:4.26E-7 ID:SGTC_2865|Compound:9030313|FD-Score:6.79|P-value:1.91E-11 ID:SGTC_2871|Compound:9038510|FD-Score:3.19|P-value:9.37E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.83|P-value:9.58E-5 ID:SGTC_2924|Compound:9010168|FD-Score:5.97|P-value:3.02E-9 ID:SGTC_2942|Compound:9052777|FD-Score:3.96|P-value:5.90E-5 ID:SGTC_2965|Compound:9082772|FD-Score:4.82|P-value:1.36E-6 ID:SGTC_3074|Compound:9116018|FD-Score:4.80|P-value:1.49E-6 ID:SGTC_3131|Compound:9127911|FD-Score:4.94|P-value:7.63E-7 ID:SGTC_3143|Compound:9086658|FD-Score:3.28|P-value:6.99E-4 ID:SGTC_3158|Compound:9100007|FD-Score:3.87|P-value:8.36E-5 ID:SGTC_3266|Compound:9136860|FD-Score:3.93|P-value:6.64E-5 ID:SGTC_3301|Compound:9125354|FD-Score:3.46|P-value:3.85E-4 ID:SGTC_3311|Compound:9131146|FD-Score:4.37|P-value:1.03E-5 ID:SGTC_3|Compound:0833-0587|FD-Score:-3.32|P-value:6.07E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.34|P-value:1.20E-5 ID:SGTC_17|Compound:0960-0276|FD-Score:3.79|P-value:1.11E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.21|P-value:2.08E-5 ID:SGTC_92|Compound:0063-0024|FD-Score:3.65|P-value:1.90E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:17.10|P-value:1.61E-62 ID:SGTC_223|Compound:busulfan|FD-Score:3.44|P-value:4.02E-4 ID:SGTC_246|Compound:cycloheximide|FD-Score:3.58|P-value:2.42E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.68|P-value:1.68E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:-4.50|P-value:5.74E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.51|P-value:5.59E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:6.86|P-value:1.21E-11 ID:SGTC_342|Compound:k048-0088|FD-Score:3.55|P-value:2.72E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.29|P-value:6.85E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:8.35|P-value:2.14E-16 ID:SGTC_662|Compound:k832-1615|FD-Score:3.67|P-value:1.74E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:4.88|P-value:9.97E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:8.17|P-value:8.47E-16 ID:SGTC_738|Compound:1315-0003|FD-Score:-4.50|P-value:5.80E-6 ID:SGTC_829|Compound:1635-0120|FD-Score:3.25|P-value:7.93E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:5.06|P-value:4.21E-7 ID:SGTC_883|Compound:0850-0245|FD-Score:4.83|P-value:1.30E-6 ID:SGTC_951|Compound:1171-0579|FD-Score:8.24|P-value:5.17E-16 ID:SGTC_970|Compound:1315-0089|FD-Score:4.11|P-value:3.11E-5 ID:SGTC_977|Compound:1348-1378|FD-Score:4.32|P-value:1.29E-5 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.12|P-value:3.05E-5 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.23|P-value:8.29E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:4.32|P-value:1.29E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.28|P-value:6.79E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:5.12|P-value:3.10E-7 ID:SGTC_1209|Compound:2323-2347|FD-Score:4.39|P-value:9.53E-6 ID:SGTC_1215|Compound:3852-0577|FD-Score:5.00|P-value:5.64E-7 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.52|P-value:3.09E-4 ID:SGTC_1605|Compound:st000082|FD-Score:-3.66|P-value:1.76E-4 ID:SGTC_1606|Compound:st000094|FD-Score:4.67|P-value:2.72E-6 ID:SGTC_1621|Compound:st003705|FD-Score:3.65|P-value:1.89E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:-3.22|P-value:8.39E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:3.21|P-value:8.84E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.38|P-value:4.99E-4 ID:SGTC_1950|Compound:st077106|FD-Score:3.66|P-value:1.83E-4 ID:SGTC_2049|Compound:5262831|FD-Score:6.55|P-value:8.66E-11 ID:SGTC_2054|Compound:5233173|FD-Score:3.51|P-value:3.18E-4 ID:SGTC_2060|Compound:5238662|FD-Score:-3.40|P-value:4.47E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.49|P-value:3.45E-4 ID:SGTC_2268|Compound:7933382|FD-Score:3.58|P-value:2.48E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.20|P-value:9.17E-4 ID:SGTC_2315|Compound:7733312|FD-Score:4.36|P-value:1.12E-5 ID:SGTC_2412|Compound:st077135|FD-Score:-4.88|P-value:9.60E-7 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.91|P-value:7.01E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:-3.44|P-value:3.92E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.46|P-value:3.77E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:9.86|P-value:3.95E-22 ID:SGTC_2711|Compound:st079103|FD-Score:3.53|P-value:2.98E-4 ID:SGTC_2837|Compound:9008476|FD-Score:4.42|P-value:8.25E-6 ID:SGTC_2840|Compound:9008771|FD-Score:-3.86|P-value:8.22E-5 ID:SGTC_2844|Compound:9010103|FD-Score:5.06|P-value:4.28E-7 ID:SGTC_2845|Compound:9003024|FD-Score:5.06|P-value:4.26E-7 ID:SGTC_2865|Compound:9030313|FD-Score:6.79|P-value:1.91E-11 ID:SGTC_2871|Compound:9038510|FD-Score:3.19|P-value:9.37E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.83|P-value:9.58E-5 ID:SGTC_2924|Compound:9010168|FD-Score:5.97|P-value:3.02E-9 ID:SGTC_2942|Compound:9052777|FD-Score:3.96|P-value:5.90E-5 ID:SGTC_2965|Compound:9082772|FD-Score:4.82|P-value:1.36E-6 ID:SGTC_3074|Compound:9116018|FD-Score:4.80|P-value:1.49E-6 ID:SGTC_3131|Compound:9127911|FD-Score:4.94|P-value:7.63E-7 ID:SGTC_3143|Compound:9086658|FD-Score:3.28|P-value:6.99E-4 ID:SGTC_3158|Compound:9100007|FD-Score:3.87|P-value:8.36E-5 ID:SGTC_3266|Compound:9136860|FD-Score:3.93|P-value:6.64E-5 ID:SGTC_3301|Compound:9125354|FD-Score:3.46|P-value:3.85E-4 ID:SGTC_3311|Compound:9131146|FD-Score:4.37|P-value:1.03E-5

Top fitness defect scores for YJL073W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_138 0850-0259 17.10 40.9 µM 1.61E-62 cell wall 11 9.44E-66
2 SGTC_2584 androsterone 9.86 48.7 µM 3.95E-22 cell wall 17 3.22E-23
3 SGTC_514 4466-0038 8.35 2.6 nM 2.14E-16 cell wall 33 3.50E-17
4 SGTC_951 1171-0579 8.24 82.7 µM 5.17E-16 cell wall 13 8.84E-17
5 SGTC_690 glyoxal 8.17 20.3 µM 8.47E-16 27 1.49E-16
6 SGTC_304 1310-0298 6.86 97.0 µM 1.21E-11 cell wall 11 3.51E-12
7 SGTC_2865 9030313 6.79 52.0 µM 1.91E-11 2 5.67E-12
8 SGTC_2049 5262831 6.55 67.2 µM 8.66E-11 3 2.79E-11
9 SGTC_2924 9010168 5.97 62.8 µM 3.02E-9 cell wall signaling 9 1.17E-9
10 SGTC_1132 3456-3614 5.12 2.5 µM 3.10E-7 tubulin folding & SWR complex 15 1.53E-7
11 SGTC_863 0466-0191 5.06 129.0 µM 4.21E-7 cell wall 44 2.11E-7
12 SGTC_2845 9003024 5.06 43.0 µM 4.26E-7 amide catabolism 24 2.14E-7
13 SGTC_2844 9010103 5.06 26.0 µM 4.28E-7 8 2.15E-7
14 SGTC_1215 3852-0577 5.00 11.2 µM 5.64E-7 7 2.88E-7
15 SGTC_3131 9127911 4.94 49.5 µM 7.63E-7 9 3.95E-7
16 SGTC_672 1441-0018 4.88 53.7 µM 9.97E-7 4 5.24E-7
17 SGTC_883 0850-0245 4.83 24.6 µM 1.30E-6 cell wall 15 6.91E-7
18 SGTC_2965 9082772 4.82 38.6 µM 1.36E-6 mitochondrial response to ROS 23 7.25E-7
19 SGTC_3074 9116018 4.80 49.5 µM 1.49E-6 RNA processing & uracil transport 9 8.00E-7
20 SGTC_1606 st000094 4.67 49.2 µM 2.72E-6 60S ribosome export 6 1.51E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.378 5.64E-114 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.374 1.09E-111 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.373 3.24E-111 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.343 4.07E-93 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.340 1.30E-91 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.338 3.45E-90 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.336 2.13E-89 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.333 1.63E-87 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.325 3.30E-83 YJR111C_p YJR111C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.318 1.76E-79 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.317 3.43E-79 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.308 2.77E-74 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.304 1.10E-72 YDL021W GPM2 hom Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
0.303 3.36E-72 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.295 1.33E-68 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria