YJL077W-B_p

Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL077W-B / YJL077W-B

Click on Significant Values for Screen Details ID:SGTC_236|Compound:1509-0011|FD-Score:4.13|P-value:8.34E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:-3.66|P-value:2.00E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:4.84|P-value:4.76E-6 ID:SGTC_865|Compound:0488-0499|FD-Score:-3.70|P-value:1.78E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:5.52|P-value:2.03E-7 ID:SGTC_1086|Compound:cerivastatin|FD-Score:-3.50|P-value:3.60E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:-3.26|P-value:7.77E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.26|P-value:7.76E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:5.94|P-value:2.41E-8 ID:SGTC_1132|Compound:3456-3614|FD-Score:-3.28|P-value:7.44E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.98|P-value:6.16E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.98|P-value:6.38E-5 ID:SGTC_1634|Compound:st006576|FD-Score:-4.13|P-value:3.48E-5 ID:SGTC_1635|Compound:st007035|FD-Score:5.50|P-value:2.24E-7 ID:SGTC_1957|Compound:st077035|FD-Score:3.64|P-value:4.85E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.31|P-value:6.76E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-5.13|P-value:4.58E-7 ID:SGTC_2754|Compound:granisetron|FD-Score:3.98|P-value:1.47E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:-3.87|P-value:9.36E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-4.24|P-value:2.23E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:4.13|P-value:8.34E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:-3.66|P-value:2.00E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:4.84|P-value:4.76E-6 ID:SGTC_865|Compound:0488-0499|FD-Score:-3.70|P-value:1.78E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:5.52|P-value:2.03E-7 ID:SGTC_1086|Compound:cerivastatin|FD-Score:-3.50|P-value:3.60E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:-3.26|P-value:7.77E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.26|P-value:7.76E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:5.94|P-value:2.41E-8 ID:SGTC_1132|Compound:3456-3614|FD-Score:-3.28|P-value:7.44E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.98|P-value:6.16E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.98|P-value:6.38E-5 ID:SGTC_1634|Compound:st006576|FD-Score:-4.13|P-value:3.48E-5 ID:SGTC_1635|Compound:st007035|FD-Score:5.50|P-value:2.24E-7 ID:SGTC_1957|Compound:st077035|FD-Score:3.64|P-value:4.85E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.31|P-value:6.76E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-5.13|P-value:4.58E-7 ID:SGTC_2754|Compound:granisetron|FD-Score:3.98|P-value:1.47E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:-3.87|P-value:9.36E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-4.24|P-value:2.23E-5

Top fitness defect scores for YJL077W-B deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1123 mangostin 5.94 811.3 nM 2.41E-8 mitochondrial response to ROS 12 1.40E-9
2 SGTC_1082 camptothecin 5.52 424.9 nM 2.03E-7 DNA damage response 23 1.66E-8
3 SGTC_1635 st007035 5.50 42.4 µM 2.24E-7 147 1.86E-8
4 SGTC_802 4112-3352 4.84 742.0 µM 4.76E-6 10 6.40E-7
5 SGTC_236 1509-0011 4.13 5.3 µM 8.34E-5 373 1.78E-5
6 SGTC_2754 granisetron 3.98 64.0 µM 1.47E-4 29 3.42E-5
7 SGTC_1957 st077035 3.64 62.4 µM 4.85E-4 23 1.38E-4
8 SGTC_3097 9117246 3.39 49.5 µM 0.00107 25 3.45E-4
9 SGTC_849 0386-0493 3.32 241.0 µM 0.00135 37 4.54E-4
10 SGTC_1362 1611-0397 3.25 101.0 µM 0.00168 42 5.84E-4
11 SGTC_1967 st070177 3.24 51.4 µM 0.00173 43 6.04E-4
12 SGTC_3098 9119039 3.24 49.5 µM 0.00173 Golgi 63 6.04E-4
13 SGTC_3348 9149464 3.21 3.5 µM 0.00189 44 6.72E-4
14 SGTC_2109 5483026 3.16 200.0 µM 0.00220 RSC complex & mRNA processing 67 8.02E-4
15 SGTC_752 1896-0090 3.11 130.0 µM 0.00249 TSC3-RPN4 44 9.23E-4
16 SGTC_2844 9010103 3.10 26.0 µM 0.00257 75 9.60E-4
17 SGTC_2544 sphondin 3.10 68.5 µM 0.00261 RNA pol III & RNase P/MRP 50 9.74E-4
18 SGTC_2868 9035608 3.09 32.5 µM 0.00268 47 0.00101
19 SGTC_1725 st036284 3.06 20.6 µM 0.00288 67 0.00109
20 SGTC_2116 5511452 3.06 16.9 µM 0.00290 50 0.00111

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.259 9.96E-53 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.252 6.41E-50 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.245 3.42E-47 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.245 4.24E-47 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.239 5.60E-45 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.237 3.96E-44 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.234 4.51E-43 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.221 1.54E-38 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.221 2.14E-38 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.221 3.62E-38 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.218 2.20E-37 YGL191W COX13 hom Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.215 2.69E-36 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.214 4.88E-36 YDL107W MSS2 hom Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
0.212 2.36E-35 YLR038C COX12 hom Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV
0.211 5.14E-35 YML129C COX14 hom Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes