YJL084C / ALY2

Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL084C / ALY2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.64|P-value:4.92E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:2.89|P-value:9.87E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:-3.62|P-value:6.03E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-3.52|P-value:9.42E-5 ID:SGTC_272|Compound:doxorubicin|FD-Score:3.35|P-value:1.68E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:2.93|P-value:8.32E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.07|P-value:4.90E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.93|P-value:1.31E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:3.27|P-value:2.28E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.03|P-value:5.73E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.16|P-value:3.57E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:4.10|P-value:5.80E-6 ID:SGTC_535|Compound:2293-4129|FD-Score:3.02|P-value:6.10E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:-3.05|P-value:6.09E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.38|P-value:1.46E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:4.56|P-value:5.32E-7 ID:SGTC_695|Compound:3505-6186|FD-Score:2.98|P-value:7.02E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:-4.46|P-value:1.06E-6 ID:SGTC_730|Compound:1436-0052|FD-Score:-3.06|P-value:5.85E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.77|P-value:2.74E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.92|P-value:1.56E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:3.83|P-value:2.08E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.06|P-value:5.93E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-3.20|P-value:3.46E-4 ID:SGTC_1628|Compound:st004252|FD-Score:3.13|P-value:3.92E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-2.94|P-value:9.21E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.13|P-value:3.99E-4 ID:SGTC_2060|Compound:5238662|FD-Score:3.17|P-value:3.38E-4 ID:SGTC_2158|Compound:5657123|FD-Score:3.73|P-value:3.30E-5 ID:SGTC_2167|Compound:5876490|FD-Score:3.33|P-value:1.79E-4 ID:SGTC_2182|Compound:6325192|FD-Score:2.97|P-value:7.30E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.12|P-value:4.79E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.86|P-value:1.78E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.92|P-value:1.35E-5 ID:SGTC_2694|Compound:st077685|FD-Score:3.02|P-value:5.99E-4 ID:SGTC_2701|Compound:st077767|FD-Score:-4.28|P-value:2.69E-6 ID:SGTC_2707|Compound:st078556|FD-Score:3.84|P-value:1.92E-5 ID:SGTC_2708|Compound:st078559|FD-Score:3.58|P-value:6.18E-5 ID:SGTC_2716|Compound:st080858|FD-Score:3.36|P-value:1.56E-4 ID:SGTC_2864|Compound:9034755|FD-Score:-3.00|P-value:7.41E-4 ID:SGTC_2867|Compound:9039098|FD-Score:-3.21|P-value:3.26E-4 ID:SGTC_2960|Compound:9079389|FD-Score:3.14|P-value:3.76E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.64|P-value:4.92E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:2.89|P-value:9.87E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:-3.62|P-value:6.03E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-3.52|P-value:9.42E-5 ID:SGTC_272|Compound:doxorubicin|FD-Score:3.35|P-value:1.68E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:2.93|P-value:8.32E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.07|P-value:4.90E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.93|P-value:1.31E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:3.27|P-value:2.28E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.03|P-value:5.73E-4 ID:SGTC_481|Compound:bay k-8644|FD-Score:3.16|P-value:3.57E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:4.10|P-value:5.80E-6 ID:SGTC_535|Compound:2293-4129|FD-Score:3.02|P-value:6.10E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:-3.05|P-value:6.09E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.38|P-value:1.46E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:4.56|P-value:5.32E-7 ID:SGTC_695|Compound:3505-6186|FD-Score:2.98|P-value:7.02E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:-4.46|P-value:1.06E-6 ID:SGTC_730|Compound:1436-0052|FD-Score:-3.06|P-value:5.85E-4 ID:SGTC_816|Compound:0325-0417|FD-Score:3.77|P-value:2.74E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:-3.92|P-value:1.56E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:3.83|P-value:2.08E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.06|P-value:5.93E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-3.20|P-value:3.46E-4 ID:SGTC_1628|Compound:st004252|FD-Score:3.13|P-value:3.92E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-2.94|P-value:9.21E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.13|P-value:3.99E-4 ID:SGTC_2060|Compound:5238662|FD-Score:3.17|P-value:3.38E-4 ID:SGTC_2158|Compound:5657123|FD-Score:3.73|P-value:3.30E-5 ID:SGTC_2167|Compound:5876490|FD-Score:3.33|P-value:1.79E-4 ID:SGTC_2182|Compound:6325192|FD-Score:2.97|P-value:7.30E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.12|P-value:4.79E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.86|P-value:1.78E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.92|P-value:1.35E-5 ID:SGTC_2694|Compound:st077685|FD-Score:3.02|P-value:5.99E-4 ID:SGTC_2701|Compound:st077767|FD-Score:-4.28|P-value:2.69E-6 ID:SGTC_2707|Compound:st078556|FD-Score:3.84|P-value:1.92E-5 ID:SGTC_2708|Compound:st078559|FD-Score:3.58|P-value:6.18E-5 ID:SGTC_2716|Compound:st080858|FD-Score:3.36|P-value:1.56E-4 ID:SGTC_2864|Compound:9034755|FD-Score:-3.00|P-value:7.41E-4 ID:SGTC_2867|Compound:9039098|FD-Score:-3.21|P-value:3.26E-4 ID:SGTC_2960|Compound:9079389|FD-Score:3.14|P-value:3.76E-4

Top fitness defect scores for YJL084C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_618 k072-0232 4.56 33.2 µM 5.32E-7 60S ribosome export 3 2.55E-6
2 SGTC_533 1222-0222 4.10 6.7 µM 5.80E-6 PDR1 24 2.10E-5
3 SGTC_309 k408-0051 3.93 1.5 µM 1.31E-5 47 4.30E-5
4 SGTC_2693 st077581 3.92 40.7 µM 1.35E-5 34 4.41E-5
5 SGTC_2583 asiatic acid 3.86 100.0 µM 1.78E-5 40 5.63E-5
6 SGTC_2707 st078556 3.84 66.6 µM 1.92E-5 13 6.04E-5
7 SGTC_913 3487-1245 3.83 167.0 µM 2.08E-5 34 6.46E-5
8 SGTC_816 0325-0417 3.77 81.3 µM 2.74E-5 15 8.26E-5
9 SGTC_2158 5657123 3.73 3.6 µM 3.30E-5 10 9.74E-5
10 SGTC_58 0957-0393 3.64 9.0 µM 4.92E-5 cell wall 410 1.38E-4
11 SGTC_2708 st078559 3.58 56.0 µM 6.18E-5 15 1.69E-4
12 SGTC_552 k242-0005 3.38 476.0 µM 1.46E-4 36 3.62E-4
13 SGTC_2716 st080858 3.36 38.6 µM 1.56E-4 60S ribosome export 33 3.83E-4
14 SGTC_272 doxorubicin 3.35 8.1 µM 1.68E-4 anthracycline transcription coupled DNA repair 44 4.08E-4
15 SGTC_2167 5876490 3.33 200.0 µM 1.79E-4 57 4.33E-4
16 SGTC_310 1273-0058 3.27 1.1 µM 2.28E-4 mitochondrial stress 315 5.35E-4
17 SGTC_2060 5238662 3.17 140.0 µM 3.38E-4 55 7.58E-4
18 SGTC_481 bay k-8644 3.16 50.0 µM 3.57E-4 56 7.96E-4
19 SGTC_2960 9079389 3.14 6.0 µM 3.76E-4 62 8.32E-4
20 SGTC_1628 st004252 3.13 77.7 µM 3.92E-4 58 8.64E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.165 9.10E-22 YOL053W AIM39 hom Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss
0.161 7.59E-21 YJL153C INO1 hom Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
0.142 1.61E-16 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.138 9.68E-16 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.134 6.65E-15 YIR003W AIM21 hom Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton
0.123 7.22E-13 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.122 1.23E-12 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.122 1.25E-12 YOR292C_p YOR292C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene
0.116 1.67E-11 YIL123W SIM1 hom Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
0.115 2.51E-11 YOR093C_p YOR093C_p hom Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents
0.114 3.91E-11 YLR412W BER1 hom Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
0.109 2.16E-10 YNL291C MID1 hom N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
0.108 3.86E-10 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
0.107 6.14E-10 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.105 1.02E-9 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids