YJL085W / EXO70

Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL085W / EXO70

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:3.25|P-value:4.79E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:3.77|P-value:6.41E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:3.87|P-value:4.15E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:5.21|P-value:5.43E-8 ID:SGTC_144|Compound:4161-2749|FD-Score:4.51|P-value:2.17E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:6.80|P-value:1.95E-12 ID:SGTC_146|Compound:3448-8650|FD-Score:3.66|P-value:9.92E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:3.18|P-value:6.16E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.30|P-value:4.07E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:3.46|P-value:2.16E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:4.20|P-value:9.64E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:6.19|P-value:1.40E-10 ID:SGTC_320|Compound:4092-0846|FD-Score:4.39|P-value:3.93E-6 ID:SGTC_389|Compound:tpck|FD-Score:3.05|P-value:9.76E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.26|P-value:4.69E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.13|P-value:1.31E-5 ID:SGTC_470|Compound:aa-861|FD-Score:4.80|P-value:4.98E-7 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.75|P-value:6.83E-5 ID:SGTC_512|Compound:prima-1|FD-Score:3.41|P-value:2.66E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.06|P-value:7.59E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.95|P-value:2.97E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:4.09|P-value:1.58E-5 ID:SGTC_569|Compound:4130-2812|FD-Score:4.12|P-value:1.39E-5 ID:SGTC_615|Compound:k087-0205|FD-Score:-3.33|P-value:2.89E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:3.34|P-value:3.50E-4 ID:SGTC_675|Compound:1309-0189|FD-Score:-3.53|P-value:1.30E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:4.99|P-value:1.82E-7 ID:SGTC_739|Compound:1486-1183|FD-Score:5.19|P-value:6.24E-8 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.65|P-value:1.01E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.43|P-value:2.46E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.98|P-value:2.61E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:4.15|P-value:1.21E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.67|P-value:7.35E-5 ID:SGTC_1140|Compound:3229-1848|FD-Score:4.62|P-value:1.25E-6 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.24|P-value:5.08E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.59|P-value:1.49E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:6.40|P-value:3.33E-11 ID:SGTC_1465|Compound:k213-0056|FD-Score:4.85|P-value:3.91E-7 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.69|P-value:8.69E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.06|P-value:9.54E-4 ID:SGTC_1540|Compound:guanosine|FD-Score:3.45|P-value:2.26E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.46|P-value:2.15E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:11.90|P-value:2.03E-34 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.73|P-value:5.75E-5 ID:SGTC_1722|Compound:st034304|FD-Score:3.22|P-value:5.45E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.24|P-value:4.97E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:5.98|P-value:5.48E-10 ID:SGTC_1947|Compound:st076332|FD-Score:3.76|P-value:6.57E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-3.32|P-value:2.94E-4 ID:SGTC_2156|Compound:5649887|FD-Score:5.68|P-value:3.42E-9 ID:SGTC_2249|Compound:haloperidol|FD-Score:12.80|P-value:3.36E-39 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.83|P-value:4.81E-5 ID:SGTC_2274|Compound:7947094|FD-Score:-3.12|P-value:6.25E-4 ID:SGTC_2284|Compound:7945075|FD-Score:3.49|P-value:1.91E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.98|P-value:1.96E-7 ID:SGTC_2324|Compound:6149169|FD-Score:4.85|P-value:3.91E-7 ID:SGTC_2343|Compound:7990716|FD-Score:-4.05|P-value:1.44E-5 ID:SGTC_2349|Compound:9023730|FD-Score:-3.46|P-value:1.72E-4 ID:SGTC_2353|Compound:9059630|FD-Score:-3.43|P-value:1.92E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.44|P-value:2.39E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.61|P-value:1.22E-4 ID:SGTC_2445|Compound:5742597|FD-Score:-3.32|P-value:3.01E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-7.36|P-value:1.87E-14 ID:SGTC_2458|Compound:5808907|FD-Score:3.60|P-value:1.27E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.27|P-value:6.80E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.74|P-value:5.10E-7 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.26|P-value:3.79E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.03|P-value:8.56E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:8.19|P-value:3.04E-17 ID:SGTC_2965|Compound:9082772|FD-Score:-3.07|P-value:7.36E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.93|P-value:3.17E-5 ID:SGTC_3219|Compound:9131112|FD-Score:3.12|P-value:7.60E-4 ID:SGTC_3283|Compound:9109332|FD-Score:3.20|P-value:5.69E-4 ID:SGTC_3337|Compound:9144342|FD-Score:3.10|P-value:8.33E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.25|P-value:4.79E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:3.77|P-value:6.41E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:3.87|P-value:4.15E-5 ID:SGTC_143|Compound:4032-1194|FD-Score:5.21|P-value:5.43E-8 ID:SGTC_144|Compound:4161-2749|FD-Score:4.51|P-value:2.17E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:6.80|P-value:1.95E-12 ID:SGTC_146|Compound:3448-8650|FD-Score:3.66|P-value:9.92E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:3.18|P-value:6.16E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.30|P-value:4.07E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:3.46|P-value:2.16E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:4.20|P-value:9.64E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:6.19|P-value:1.40E-10 ID:SGTC_320|Compound:4092-0846|FD-Score:4.39|P-value:3.93E-6 ID:SGTC_389|Compound:tpck|FD-Score:3.05|P-value:9.76E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:3.26|P-value:4.69E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.13|P-value:1.31E-5 ID:SGTC_470|Compound:aa-861|FD-Score:4.80|P-value:4.98E-7 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.75|P-value:6.83E-5 ID:SGTC_512|Compound:prima-1|FD-Score:3.41|P-value:2.66E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:-3.06|P-value:7.59E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.95|P-value:2.97E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:4.09|P-value:1.58E-5 ID:SGTC_569|Compound:4130-2812|FD-Score:4.12|P-value:1.39E-5 ID:SGTC_615|Compound:k087-0205|FD-Score:-3.33|P-value:2.89E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:3.34|P-value:3.50E-4 ID:SGTC_675|Compound:1309-0189|FD-Score:-3.53|P-value:1.30E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:4.99|P-value:1.82E-7 ID:SGTC_739|Compound:1486-1183|FD-Score:5.19|P-value:6.24E-8 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.65|P-value:1.01E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:3.43|P-value:2.46E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.98|P-value:2.61E-5 ID:SGTC_980|Compound:1473-0395|FD-Score:4.15|P-value:1.21E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.67|P-value:7.35E-5 ID:SGTC_1140|Compound:3229-1848|FD-Score:4.62|P-value:1.25E-6 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.24|P-value:5.08E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.59|P-value:1.49E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:6.40|P-value:3.33E-11 ID:SGTC_1465|Compound:k213-0056|FD-Score:4.85|P-value:3.91E-7 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.69|P-value:8.69E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.06|P-value:9.54E-4 ID:SGTC_1540|Compound:guanosine|FD-Score:3.45|P-value:2.26E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.46|P-value:2.15E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:11.90|P-value:2.03E-34 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.73|P-value:5.75E-5 ID:SGTC_1722|Compound:st034304|FD-Score:3.22|P-value:5.45E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.24|P-value:4.97E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:5.98|P-value:5.48E-10 ID:SGTC_1947|Compound:st076332|FD-Score:3.76|P-value:6.57E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-3.32|P-value:2.94E-4 ID:SGTC_2156|Compound:5649887|FD-Score:5.68|P-value:3.42E-9 ID:SGTC_2249|Compound:haloperidol|FD-Score:12.80|P-value:3.36E-39 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.83|P-value:4.81E-5 ID:SGTC_2274|Compound:7947094|FD-Score:-3.12|P-value:6.25E-4 ID:SGTC_2284|Compound:7945075|FD-Score:3.49|P-value:1.91E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.98|P-value:1.96E-7 ID:SGTC_2324|Compound:6149169|FD-Score:4.85|P-value:3.91E-7 ID:SGTC_2343|Compound:7990716|FD-Score:-4.05|P-value:1.44E-5 ID:SGTC_2349|Compound:9023730|FD-Score:-3.46|P-value:1.72E-4 ID:SGTC_2353|Compound:9059630|FD-Score:-3.43|P-value:1.92E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.44|P-value:2.39E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.61|P-value:1.22E-4 ID:SGTC_2445|Compound:5742597|FD-Score:-3.32|P-value:3.01E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-7.36|P-value:1.87E-14 ID:SGTC_2458|Compound:5808907|FD-Score:3.60|P-value:1.27E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.27|P-value:6.80E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.74|P-value:5.10E-7 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.26|P-value:3.79E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.03|P-value:8.56E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:8.19|P-value:3.04E-17 ID:SGTC_2965|Compound:9082772|FD-Score:-3.07|P-value:7.36E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.93|P-value:3.17E-5 ID:SGTC_3219|Compound:9131112|FD-Score:3.12|P-value:7.60E-4 ID:SGTC_3283|Compound:9109332|FD-Score:3.20|P-value:5.69E-4 ID:SGTC_3337|Compound:9144342|FD-Score:3.10|P-value:8.33E-4

Top fitness defect scores for YJL085W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2249 haloperidol 12.80 116.4 µM 3.36E-39 ergosterol depletion effects on membrane 6 1.42E-37 2.09
2 SGTC_1679 4130-1276 11.90 6.7 µM 2.03E-34 Golgi 3 5.20E-33 0.47
3 SGTC_2778 tioconazole 8.19 65.1 nM 3.04E-17 ergosterol depletion effects on membrane 9 1.32E-16 1.92
4 SGTC_145 3737-0113 6.80 38.4 µM 1.95E-12 DNA intercalators 9 5.21E-12 0.06
5 SGTC_1459 k035-0031 6.40 35.7 µM 3.33E-11 DNA intercalators 2 7.87E-11 0.88
6 SGTC_312 4092-0653 6.19 6.8 µM 1.40E-10 ergosterol depletion effects on membrane 13 3.10E-10 0.40
7 SGTC_1926 methyl fluorone black 5.98 53.6 µM 5.48E-10 DNA intercalators 10 1.15E-9 0.29
8 SGTC_2156 5649887 5.68 115.0 µM 3.42E-9 RPP1 & pyrimidine depletion 7 6.62E-9 0.55
9 SGTC_143 4032-1194 5.21 15.7 µM 5.43E-8 5 9.37E-8 0.11
10 SGTC_739 1486-1183 5.19 203.0 µM 6.24E-8 1 1.07E-7 1.00
11 SGTC_679 0422-0091 4.99 41.4 µM 1.82E-7 Golgi 4 2.98E-7 0.01
12 SGTC_2316 5844804 4.98 197.4 µM 1.96E-7 7 3.21E-7 0.15
13 SGTC_2324 6149169 4.85 200.0 µM 3.91E-7 2 6.21E-7 0.44
14 SGTC_1465 k213-0056 4.85 144.0 µM 3.91E-7 DNA intercalators 5 6.22E-7 0.40
15 SGTC_470 aa-861 4.80 153.0 µM 4.98E-7 sphingolipid biosynthesis & PDR1 9 7.84E-7 0.01
16 SGTC_1140 3229-1848 4.62 3.0 µM 1.25E-6 1 1.90E-6 0.41
17 SGTC_1290 0986-0249 4.59 10.2 µM 1.49E-6 DNA intercalators 7 2.24E-6 0.11
18 SGTC_144 4161-2749 4.51 112.4 µM 2.17E-6 DNA intercalators 9 3.22E-6 0.15
19 SGTC_320 4092-0846 4.39 5.3 µM 3.93E-6 ergosterol depletion effects on membrane 17 5.69E-6 0.31
20 SGTC_2567 aureobasidin a 4.27 60.0 nM 6.80E-6 38 9.63E-6 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.271 1.73E-57 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.256 1.85E-51 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.246 2.33E-47 YMR208W ERG12 het Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
0.243 2.32E-46 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.238 1.97E-44 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.235 3.91E-43 YGR156W PTI1 het Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.231 8.76E-42 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.223 5.72E-39 YFL005W SEC4 het Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
0.218 3.26E-37 YLR083C EMP70 hom Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments
0.208 3.68E-34 YLR171W_d YLR171W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.206 1.58E-33 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.200 1.32E-31 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.189 2.03E-28 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.184 8.31E-27 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.177 3.98E-25 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions