YJL087C / TRL1

tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL087C / TRL1

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:4.40|P-value:9.59E-8 ID:SGTC_68|Compound:0986-0284|FD-Score:3.03|P-value:1.68E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-2.91|P-value:2.77E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-2.80|P-value:4.50E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.05|P-value:1.56E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.59|P-value:1.09E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:5.27|P-value:2.24E-10 ID:SGTC_319|Compound:1348-1596|FD-Score:2.74|P-value:6.08E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:4.18|P-value:3.73E-7 ID:SGTC_338|Compound:4055-0136|FD-Score:-2.68|P-value:7.26E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:-2.72|P-value:6.30E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-2.75|P-value:5.43E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.60|P-value:1.02E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:2.80|P-value:4.75E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:2.74|P-value:6.01E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:2.66|P-value:8.30E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:4.22|P-value:2.94E-7 ID:SGTC_903|Compound:1134-0042|FD-Score:-2.86|P-value:3.48E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-2.87|P-value:3.27E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.90|P-value:3.32E-9 ID:SGTC_1002|Compound:4049-0206|FD-Score:-3.27|P-value:5.40E-5 ID:SGTC_1005|Compound:4092-0690|FD-Score:-2.76|P-value:5.39E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.20|P-value:7.57E-5 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.47|P-value:2.00E-5 ID:SGTC_1097|Compound:itavastatin|FD-Score:3.27|P-value:5.45E-5 ID:SGTC_1185|Compound:1319-0113|FD-Score:2.67|P-value:7.89E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.33|P-value:1.45E-7 ID:SGTC_1368|Compound:1852-0141|FD-Score:-3.32|P-value:4.07E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:2.64|P-value:9.14E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-4.09|P-value:6.18E-7 ID:SGTC_1491|Compound:4138-0063|FD-Score:2.68|P-value:7.78E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:2.90|P-value:2.98E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.18|P-value:8.57E-5 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.20|P-value:7.84E-5 ID:SGTC_2007|Compound:4011539|FD-Score:-3.07|P-value:1.39E-4 ID:SGTC_2011|Compound:4012611|FD-Score:-2.86|P-value:3.54E-4 ID:SGTC_2174|Compound:5790901|FD-Score:2.86|P-value:3.59E-4 ID:SGTC_2260|Compound:7964312|FD-Score:2.91|P-value:2.96E-4 ID:SGTC_2304|Compound:7443016|FD-Score:-3.07|P-value:1.38E-4 ID:SGTC_2308|Compound:7584454|FD-Score:5.75|P-value:5.29E-12 ID:SGTC_2398|Compound:5228213|FD-Score:3.64|P-value:8.25E-6 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-2.79|P-value:4.76E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.84|P-value:2.59E-6 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-4.01|P-value:1.02E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:2.84|P-value:3.98E-4 ID:SGTC_2652|Compound:actinonin|FD-Score:-2.81|P-value:4.34E-4 ID:SGTC_2691|Compound:st077356|FD-Score:2.83|P-value:4.14E-4 ID:SGTC_3171|Compound:9103192|FD-Score:-2.84|P-value:3.85E-4 ID:SGTC_3260|Compound:9137109|FD-Score:2.64|P-value:8.99E-4 ID:SGTC_57|Compound:miconazole|FD-Score:4.40|P-value:9.59E-8 ID:SGTC_68|Compound:0986-0284|FD-Score:3.03|P-value:1.68E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-2.91|P-value:2.77E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-2.80|P-value:4.50E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.05|P-value:1.56E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.59|P-value:1.09E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:5.27|P-value:2.24E-10 ID:SGTC_319|Compound:1348-1596|FD-Score:2.74|P-value:6.08E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:4.18|P-value:3.73E-7 ID:SGTC_338|Compound:4055-0136|FD-Score:-2.68|P-value:7.26E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:-2.72|P-value:6.30E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-2.75|P-value:5.43E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.60|P-value:1.02E-5 ID:SGTC_637|Compound:1181-0519|FD-Score:2.80|P-value:4.75E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:2.74|P-value:6.01E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:2.66|P-value:8.30E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:4.22|P-value:2.94E-7 ID:SGTC_903|Compound:1134-0042|FD-Score:-2.86|P-value:3.48E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-2.87|P-value:3.27E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.90|P-value:3.32E-9 ID:SGTC_1002|Compound:4049-0206|FD-Score:-3.27|P-value:5.40E-5 ID:SGTC_1005|Compound:4092-0690|FD-Score:-2.76|P-value:5.39E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.20|P-value:7.57E-5 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.47|P-value:2.00E-5 ID:SGTC_1097|Compound:itavastatin|FD-Score:3.27|P-value:5.45E-5 ID:SGTC_1185|Compound:1319-0113|FD-Score:2.67|P-value:7.89E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.33|P-value:1.45E-7 ID:SGTC_1368|Compound:1852-0141|FD-Score:-3.32|P-value:4.07E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:2.64|P-value:9.14E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-4.09|P-value:6.18E-7 ID:SGTC_1491|Compound:4138-0063|FD-Score:2.68|P-value:7.78E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:2.90|P-value:2.98E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.18|P-value:8.57E-5 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.20|P-value:7.84E-5 ID:SGTC_2007|Compound:4011539|FD-Score:-3.07|P-value:1.39E-4 ID:SGTC_2011|Compound:4012611|FD-Score:-2.86|P-value:3.54E-4 ID:SGTC_2174|Compound:5790901|FD-Score:2.86|P-value:3.59E-4 ID:SGTC_2260|Compound:7964312|FD-Score:2.91|P-value:2.96E-4 ID:SGTC_2304|Compound:7443016|FD-Score:-3.07|P-value:1.38E-4 ID:SGTC_2308|Compound:7584454|FD-Score:5.75|P-value:5.29E-12 ID:SGTC_2398|Compound:5228213|FD-Score:3.64|P-value:8.25E-6 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-2.79|P-value:4.76E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.84|P-value:2.59E-6 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-4.01|P-value:1.02E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:2.84|P-value:3.98E-4 ID:SGTC_2652|Compound:actinonin|FD-Score:-2.81|P-value:4.34E-4 ID:SGTC_2691|Compound:st077356|FD-Score:2.83|P-value:4.14E-4 ID:SGTC_3171|Compound:9103192|FD-Score:-2.84|P-value:3.85E-4 ID:SGTC_3260|Compound:9137109|FD-Score:2.64|P-value:8.99E-4

Top fitness defect scores for YJL087C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2308 7584454 5.75 197.6 µM 5.29E-12 4 4.59E-9 0.67
2 SGTC_312 4092-0653 5.27 6.8 µM 2.24E-10 ergosterol depletion effects on membrane 17 6.79E-8 0.08
3 SGTC_996 3970-0799 4.90 1.8 µM 3.32E-9 15 4.74E-7 0.02
4 SGTC_57 miconazole 4.40 162.5 nM 9.59E-8 ergosterol depletion effects on membrane 22 5.37E-6 0.21
5 SGTC_900 1112-0045 4.22 141.0 µM 2.94E-7 NEO1 5 1.21E-5 0.49
6 SGTC_321 4534-3890 4.18 65.3 µM 3.73E-7 RPP1 & pyrimidine depletion 5 1.43E-5 0.09
7 SGTC_2398 5228213 3.64 48.8 µM 8.25E-6 iron homeostasis 17 1.35E-4 0.10
8 SGTC_636 0kpi-0074 3.60 126.0 µM 1.02E-5 5 1.58E-4 0.07
9 SGTC_216 k038-0035 3.59 28.4 µM 1.09E-5 ubiquinone biosynthesis & proteasome 19 1.65E-4 0.02
10 SGTC_1034 k072-0165 3.47 12.7 µM 2.00E-5 Golgi 8 2.57E-4 0.04
11 SGTC_1097 itavastatin 3.27 7.2 µM 5.45E-5 azole & statin 13 5.34E-4 0.06
12 SGTC_1032 k061-1018 3.20 98.7 µM 7.57E-5 14 6.78E-4 0.23
13 SGTC_1692 fluoroquinolonic acid 3.20 71.0 µM 7.84E-5 4 6.96E-4 0.17
14 SGTC_1562 morin hydrate 3.18 59.1 µM 8.57E-5 14 7.42E-4 0.04
15 SGTC_207 4185-0010 3.05 4.5 µM 1.56E-4 endomembrane recycling 32 0.00115 0.04
16 SGTC_68 0986-0284 3.03 82.8 µM 1.68E-4 24 0.00121 0.01
17 SGTC_2260 7964312 2.91 200.0 µM 2.96E-4 21 0.00184 0.00
18 SGTC_1507 4075-3230 2.90 155.0 µM 2.98E-4 8 0.00185 0.10
19 SGTC_2174 5790901 2.86 200.0 µM 3.59E-4 tubulin folding & SWR complex 25 0.00211 0.01
20 SGTC_2629 bisabolol 2.84 100.0 µM 3.98E-4 25 0.00228 5.39E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.242 4.81E-46 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.208 5.33E-34 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.193 1.84E-29 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.184 6.45E-27 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.182 1.59E-26 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.181 3.32E-26 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.180 7.22E-26 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.177 7.19E-25 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.176 1.02E-24 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.173 6.22E-24 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.162 3.45E-21 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.157 5.78E-20 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.154 3.69E-19 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.148 6.78E-18 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.148 6.99E-18 YLR367W RPS22B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication