YJL090C / DPB11

DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL090C / DPB11

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:5.31|P-value:8.94E-8 ID:SGTC_134|Compound:0269-0018|FD-Score:3.56|P-value:2.42E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.17|P-value:9.52E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.28|P-value:5.33E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.41|P-value:3.40E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-6.35|P-value:1.40E-10 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.35|P-value:5.21E-8 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-3.49|P-value:2.49E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.11|P-value:9.68E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.26|P-value:1.39E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:5.81|P-value:5.48E-9 ID:SGTC_818|Compound:4072-2707|FD-Score:3.62|P-value:1.87E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.51|P-value:2.91E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:-3.25|P-value:6.00E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.27|P-value:5.62E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:-3.94|P-value:4.42E-5 ID:SGTC_1196|Compound:1588-0294|FD-Score:-3.89|P-value:5.48E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.88|P-value:7.04E-5 ID:SGTC_1202|Compound:1988-0068|FD-Score:-3.11|P-value:9.68E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:-5.08|P-value:2.27E-7 ID:SGTC_1218|Compound:1348-1416|FD-Score:4.95|P-value:5.75E-7 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.37|P-value:4.74E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:4.47|P-value:5.69E-6 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:3.31|P-value:5.87E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:4.78|P-value:1.33E-6 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.50|P-value:4.93E-6 ID:SGTC_2185|Compound:6113692|FD-Score:3.45|P-value:3.59E-4 ID:SGTC_2188|Compound:6126565|FD-Score:-3.33|P-value:4.54E-4 ID:SGTC_2210|Compound:7115885|FD-Score:3.41|P-value:4.06E-4 ID:SGTC_2226|Compound:7298446|FD-Score:-3.25|P-value:5.98E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.47|P-value:3.27E-4 ID:SGTC_2312|Compound:7706453|FD-Score:4.19|P-value:1.91E-5 ID:SGTC_2426|Compound:5582046|FD-Score:4.90|P-value:7.30E-7 ID:SGTC_2488|Compound:5268135|FD-Score:-6.14|P-value:5.36E-10 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:3.32|P-value:5.62E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.03|P-value:3.72E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.38|P-value:4.61E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.17|P-value:9.38E-4 ID:SGTC_2646|Compound:retinol|FD-Score:4.23|P-value:1.63E-5 ID:SGTC_2710|Compound:st078562|FD-Score:3.19|P-value:8.77E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.94|P-value:5.51E-5 ID:SGTC_2801|Compound:7907800|FD-Score:-3.25|P-value:5.92E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-3.38|P-value:3.75E-4 ID:SGTC_2850|Compound:9015429|FD-Score:3.42|P-value:3.90E-4 ID:SGTC_2867|Compound:9039098|FD-Score:-3.32|P-value:4.65E-4 ID:SGTC_2941|Compound:9056145|FD-Score:5.02|P-value:3.99E-7 ID:SGTC_3068|Compound:9116768|FD-Score:-4.02|P-value:3.11E-5 ID:SGTC_3131|Compound:9127911|FD-Score:3.61|P-value:1.97E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-5.04|P-value:2.69E-7 ID:SGTC_36|Compound:0335-0881|FD-Score:5.31|P-value:8.94E-8 ID:SGTC_134|Compound:0269-0018|FD-Score:3.56|P-value:2.42E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.17|P-value:9.52E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.28|P-value:5.33E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.41|P-value:3.40E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-6.35|P-value:1.40E-10 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.35|P-value:5.21E-8 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:-3.49|P-value:2.49E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.11|P-value:9.68E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.26|P-value:1.39E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:5.81|P-value:5.48E-9 ID:SGTC_818|Compound:4072-2707|FD-Score:3.62|P-value:1.87E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.51|P-value:2.91E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:-3.25|P-value:6.00E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.27|P-value:5.62E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:-3.94|P-value:4.42E-5 ID:SGTC_1196|Compound:1588-0294|FD-Score:-3.89|P-value:5.48E-5 ID:SGTC_1199|Compound:1638-0010|FD-Score:3.88|P-value:7.04E-5 ID:SGTC_1202|Compound:1988-0068|FD-Score:-3.11|P-value:9.68E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:-5.08|P-value:2.27E-7 ID:SGTC_1218|Compound:1348-1416|FD-Score:4.95|P-value:5.75E-7 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.37|P-value:4.74E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:4.47|P-value:5.69E-6 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:3.31|P-value:5.87E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:4.78|P-value:1.33E-6 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.50|P-value:4.93E-6 ID:SGTC_2185|Compound:6113692|FD-Score:3.45|P-value:3.59E-4 ID:SGTC_2188|Compound:6126565|FD-Score:-3.33|P-value:4.54E-4 ID:SGTC_2210|Compound:7115885|FD-Score:3.41|P-value:4.06E-4 ID:SGTC_2226|Compound:7298446|FD-Score:-3.25|P-value:5.98E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.47|P-value:3.27E-4 ID:SGTC_2312|Compound:7706453|FD-Score:4.19|P-value:1.91E-5 ID:SGTC_2426|Compound:5582046|FD-Score:4.90|P-value:7.30E-7 ID:SGTC_2488|Compound:5268135|FD-Score:-6.14|P-value:5.36E-10 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:3.32|P-value:5.62E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.03|P-value:3.72E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:3.38|P-value:4.61E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.17|P-value:9.38E-4 ID:SGTC_2646|Compound:retinol|FD-Score:4.23|P-value:1.63E-5 ID:SGTC_2710|Compound:st078562|FD-Score:3.19|P-value:8.77E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.94|P-value:5.51E-5 ID:SGTC_2801|Compound:7907800|FD-Score:-3.25|P-value:5.92E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-3.38|P-value:3.75E-4 ID:SGTC_2850|Compound:9015429|FD-Score:3.42|P-value:3.90E-4 ID:SGTC_2867|Compound:9039098|FD-Score:-3.32|P-value:4.65E-4 ID:SGTC_2941|Compound:9056145|FD-Score:5.02|P-value:3.99E-7 ID:SGTC_3068|Compound:9116768|FD-Score:-4.02|P-value:3.11E-5 ID:SGTC_3131|Compound:9127911|FD-Score:3.61|P-value:1.97E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-5.04|P-value:2.69E-7

Top fitness defect scores for YJL090C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_815 1582-0027 5.81 36.9 µM 5.48E-9 1 3.18E-9 1.00
2 SGTC_36 0335-0881 5.31 14.2 µM 8.94E-8 ubiquinone biosynthesis & proteasome 19 5.59E-8 0.04
3 SGTC_2941 9056145 5.02 11.2 µM 3.99E-7 4 2.60E-7 0.42
4 SGTC_1218 1348-1416 4.95 30.7 µM 5.75E-7 SWF1 & branched chain AA biosynthesis 1 3.79E-7 0.67
5 SGTC_2426 5582046 4.90 32.2 µM 7.30E-7 2 4.84E-7 0.57
6 SGTC_1562 morin hydrate 4.78 59.1 µM 1.33E-6 4 8.96E-7 0.30
7 SGTC_1574 spiramycin 4.50 23.7 µM 4.93E-6 2 3.44E-6 0.08
8 SGTC_1543 azetidyl-2-carboxylic acid 4.47 198.0 µM 5.69E-6 1 3.99E-6 0.80
9 SGTC_670 4204-0025 4.26 119.0 µM 1.39E-5 copper-dependent oxidative stress 2 1.00E-5 0.51
10 SGTC_2646 retinol 4.23 96.8 µM 1.63E-5 1 1.18E-5 0.33
11 SGTC_2312 7706453 4.19 155.7 µM 1.91E-5 6 1.39E-5 0.10
12 SGTC_2533 agelasine 4.03 13.7 µM 3.72E-5 ERG2 12 2.74E-5 0.01
13 SGTC_2796 7949634 3.94 45.5 µM 5.51E-5 6 4.11E-5 0.00
14 SGTC_1199 1638-0010 3.88 4.5 µM 7.04E-5 endomembrane recycling 4 5.29E-5 0.43
15 SGTC_818 4072-2707 3.62 53.5 µM 1.87E-4 11 1.45E-4 0.10
16 SGTC_3131 9127911 3.61 49.5 µM 1.97E-4 10 1.53E-4 0.31
17 SGTC_134 0269-0018 3.56 6.9 µM 2.42E-4 23 1.88E-4 0.10
18 SGTC_933 2914-0560 3.51 114.0 µM 2.91E-4 13 2.28E-4 0.23
19 SGTC_2292 7671323 3.47 168.1 µM 3.27E-4 RNA processing & uracil transport 17 2.57E-4 4.89E-5
20 SGTC_2185 6113692 3.45 200.0 µM 3.59E-4 ergosterol biosynthesis 12 2.83E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.121 1.61E-12 YJL102W MEF2 hom Mitochondrial elongation factor involved in translational elongation
0.109 2.47E-10 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.106 8.48E-10 YPR033C HTS1 het Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
0.104 1.58E-9 YML065W ORC1 het Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
0.098 1.23E-8 YOR277C_d YOR277C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20
0.095 3.70E-8 YNL271C BNI1 hom Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
0.089 2.68E-7 YNL188W KAR1 het Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p
0.086 5.63E-7 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.084 1.23E-6 YER048W-A ISD11 het Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
0.082 1.81E-6 YGR172C YIP1 het Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
0.082 2.09E-6 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.082 2.41E-6 YMR088C VBA1 hom Permease of basic amino acids in the vacuolar membrane
0.078 5.70E-6 YOL096C COQ3 hom O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.078 6.47E-6 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.077 8.49E-6 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats