YJL091C / GWT1

Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL091C / GWT1

Click on Significant Values for Screen Details ID:SGTC_60|Compound:0986-0278|FD-Score:3.05|P-value:9.47E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:3.52|P-value:1.69E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.47|P-value:2.49E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:3.17|P-value:6.33E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.05|P-value:7.72E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:3.28|P-value:4.16E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.40|P-value:2.68E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:-3.05|P-value:7.76E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:10.50|P-value:3.08E-27 ID:SGTC_539|Compound:4859-0462|FD-Score:-3.44|P-value:1.87E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.01|P-value:1.16E-7 ID:SGTC_579|Compound:4491-1009|FD-Score:3.93|P-value:3.09E-5 ID:SGTC_685|Compound:1278-0033|FD-Score:4.11|P-value:1.35E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.22|P-value:5.29E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.38|P-value:2.90E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:3.98|P-value:2.43E-5 ID:SGTC_1091|Compound:telmisartan|FD-Score:-3.39|P-value:2.21E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.49|P-value:1.86E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.72|P-value:7.56E-5 ID:SGTC_1405|Compound:3909-7894|FD-Score:-3.06|P-value:7.71E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.15|P-value:6.68E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.51|P-value:1.77E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-3.29|P-value:3.27E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.62|P-value:1.13E-4 ID:SGTC_2041|Compound:5107442|FD-Score:4.29|P-value:5.98E-6 ID:SGTC_2173|Compound:5763948|FD-Score:3.29|P-value:4.09E-4 ID:SGTC_2325|Compound:6158266|FD-Score:4.99|P-value:1.68E-7 ID:SGTC_2329|Compound:6138490|FD-Score:-3.00|P-value:9.35E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.37|P-value:2.38E-4 ID:SGTC_2458|Compound:5808907|FD-Score:3.63|P-value:1.08E-4 ID:SGTC_2461|Compound:5755302|FD-Score:4.35|P-value:4.56E-6 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:3.35|P-value:3.17E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-3.19|P-value:4.72E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.20|P-value:4.60E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.09|P-value:8.27E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-6.48|P-value:1.16E-11 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.13|P-value:5.97E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:4.05|P-value:1.78E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:4.26|P-value:6.73E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-3.07|P-value:7.24E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:3.30|P-value:3.91E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.29|P-value:3.99E-4 ID:SGTC_2983|Compound:9022671|FD-Score:4.75|P-value:6.29E-7 ID:SGTC_2997|Compound:9067075|FD-Score:6.50|P-value:1.52E-11 ID:SGTC_3007|Compound:9078383|FD-Score:3.16|P-value:6.54E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.05|P-value:9.48E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.41|P-value:2.57E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.96|P-value:2.14E-5 ID:SGTC_3203|Compound:9113543|FD-Score:3.17|P-value:6.30E-4 ID:SGTC_3301|Compound:9125354|FD-Score:3.30|P-value:3.93E-4 ID:SGTC_3344|Compound:9148862|FD-Score:3.48|P-value:1.98E-4 ID:SGTC_60|Compound:0986-0278|FD-Score:3.05|P-value:9.47E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:3.52|P-value:1.69E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.47|P-value:2.49E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:3.17|P-value:6.33E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.05|P-value:7.72E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:3.28|P-value:4.16E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.40|P-value:2.68E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:-3.05|P-value:7.76E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:10.50|P-value:3.08E-27 ID:SGTC_539|Compound:4859-0462|FD-Score:-3.44|P-value:1.87E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.01|P-value:1.16E-7 ID:SGTC_579|Compound:4491-1009|FD-Score:3.93|P-value:3.09E-5 ID:SGTC_685|Compound:1278-0033|FD-Score:4.11|P-value:1.35E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.22|P-value:5.29E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.38|P-value:2.90E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:3.98|P-value:2.43E-5 ID:SGTC_1091|Compound:telmisartan|FD-Score:-3.39|P-value:2.21E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.49|P-value:1.86E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.72|P-value:7.56E-5 ID:SGTC_1405|Compound:3909-7894|FD-Score:-3.06|P-value:7.71E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.15|P-value:6.68E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.51|P-value:1.77E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-3.29|P-value:3.27E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.62|P-value:1.13E-4 ID:SGTC_2041|Compound:5107442|FD-Score:4.29|P-value:5.98E-6 ID:SGTC_2173|Compound:5763948|FD-Score:3.29|P-value:4.09E-4 ID:SGTC_2325|Compound:6158266|FD-Score:4.99|P-value:1.68E-7 ID:SGTC_2329|Compound:6138490|FD-Score:-3.00|P-value:9.35E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.37|P-value:2.38E-4 ID:SGTC_2458|Compound:5808907|FD-Score:3.63|P-value:1.08E-4 ID:SGTC_2461|Compound:5755302|FD-Score:4.35|P-value:4.56E-6 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:3.35|P-value:3.17E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-3.19|P-value:4.72E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.20|P-value:4.60E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.09|P-value:8.27E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-6.48|P-value:1.16E-11 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.13|P-value:5.97E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:4.05|P-value:1.78E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:4.26|P-value:6.73E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-3.07|P-value:7.24E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:3.30|P-value:3.91E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.29|P-value:3.99E-4 ID:SGTC_2983|Compound:9022671|FD-Score:4.75|P-value:6.29E-7 ID:SGTC_2997|Compound:9067075|FD-Score:6.50|P-value:1.52E-11 ID:SGTC_3007|Compound:9078383|FD-Score:3.16|P-value:6.54E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.05|P-value:9.48E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.41|P-value:2.57E-4 ID:SGTC_3143|Compound:9086658|FD-Score:-3.96|P-value:2.14E-5 ID:SGTC_3203|Compound:9113543|FD-Score:3.17|P-value:6.30E-4 ID:SGTC_3301|Compound:9125354|FD-Score:3.30|P-value:3.93E-4 ID:SGTC_3344|Compound:9148862|FD-Score:3.48|P-value:1.98E-4

Top fitness defect scores for YJL091C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_531 3788-1495 10.50 174.0 µM 3.08E-27 unfolded protein response 2 4.83E-26 4.37
2 SGTC_2997 9067075 6.50 71.4 µM 1.52E-11 unfolded protein response 7 4.12E-11 0.24
3 SGTC_2325 6158266 4.99 200.0 µM 1.68E-7 3 2.95E-7 0.39
4 SGTC_2983 9022671 4.75 13.0 µM 6.29E-7 60S ribosome export 5 1.04E-6 0.04
5 SGTC_168 3970-0862 4.47 530.5 µM 2.49E-6 35 3.87E-6 0.01
6 SGTC_2461 5755302 4.35 26.1 µM 4.56E-6 2 6.90E-6 0.19
7 SGTC_2041 5107442 4.29 45.3 µM 5.98E-6 RSC complex & mRNA processing 27 8.93E-6 0.01
8 SGTC_2574 2',4'-dihydroxychalcone 4.26 14.6 µM 6.73E-6 8 1.00E-5 0.13
9 SGTC_685 1278-0033 4.11 54.9 µM 1.35E-5 60S ribosome export 7 1.94E-5 0.02
10 SGTC_2543 pimpinellin 4.05 60.7 µM 1.78E-5 Golgi 4 2.53E-5 0.39
11 SGTC_999 4022-0066 3.98 22.7 µM 2.43E-5 Golgi 11 3.41E-5 0.06
12 SGTC_579 4491-1009 3.93 110.0 µM 3.09E-5 60S ribosome export 7 4.29E-5 0.19
13 SGTC_1368 1852-0141 3.72 284.0 µM 7.56E-5 ERG2 5 1.01E-4 0.25
14 SGTC_2458 5808907 3.63 16.4 µM 1.08E-4 5 1.42E-4 0.03
15 SGTC_1740 st038286 3.62 13.2 µM 1.13E-4 11 1.48E-4 0.13
16 SGTC_104 0074-0074 3.52 591.0 µM 1.69E-4 21 2.18E-4 0.03
17 SGTC_1550 gossypolone 3.51 36.6 µM 1.77E-4 7 2.27E-4 0.06
18 SGTC_1306 1185-0270 3.49 9.0 µM 1.86E-4 Golgi 14 2.40E-4 0.14
19 SGTC_3344 9148862 3.48 3.9 µM 1.98E-4 5 2.53E-4 0.02
20 SGTC_3142 9086498 3.41 49.5 µM 2.57E-4 iron homeostasis 54 3.26E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.168 1.67E-22 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.137 1.45E-15 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.127 1.61E-13 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.114 3.61E-11 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.114 3.69E-11 YDL131W LYS21 hom Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
0.102 3.27E-9 YMR294W-A_d YMR294W-A_d hom Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents
0.102 3.49E-9 YLR163C MAS1 het Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
0.101 5.41E-9 YOR216C RUD3 hom Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
0.095 4.19E-8 YKL063C_p YKL063C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
0.091 1.58E-7 YKL182W FAS1 het Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
0.090 2.01E-7 YGL027C CWH41 hom Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
0.089 2.54E-7 YLL053C_p YLL053C_p hom Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin
0.088 3.17E-7 YDR170C SEC7 het Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
0.088 4.06E-7 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.087 4.29E-7 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus