Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YJL095W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.777 | 0 | YHR030C | SLT2 | hom | Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway |
0.398 | 1.88E-127 | YLR332W | MID2 | hom | O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p |
0.387 | 4.07E-120 | YPR165W | RHO1 | het | GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) |
0.364 | 1.00E-105 | YDL203C | ACK1 | hom | Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria |
0.328 | 9.42E-85 | YPL089C | RLM1 | hom | MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication |
0.322 | 5.70E-82 | YLR331C_d | JIP3_d | hom | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 |
0.311 | 5.18E-76 | YGR196C | FYV8 | hom | Protein of unknown function, required for survival upon exposure to K1 killer toxin |
0.284 | 4.12E-63 | YNL322C | KRE1 | hom | Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor |
0.269 | 1.60E-56 | YNL084C | END3 | hom | EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p |
0.266 | 2.89E-55 | YOR008C | SLG1 | hom | Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response |
0.260 | 5.39E-53 | YHR209W | CRG1 | hom | S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin |
0.259 | 1.02E-52 | YLL043W | FPS1 | hom | Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress |
0.257 | 7.36E-52 | YJL099W | CHS6 | hom | Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication |
0.249 | 8.35E-49 | YBR260C | RGD1 | hom | GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization |
0.240 | 2.32E-45 | YNL079C | TPM1 | hom | Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication |