HIPHOP chemogenomics database

YJL095W / BCK1

Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL095W / BCK1

Click on Significant Values for Screen Details

Top fitness defect scores for YJL095W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_422	cantharidin	43.90	155.0 µM	1.36E-187	cell wall signaling	1	0
2	SGTC_2272	7805186	43.50	180.0 µM	2.60E-184	cell wall signaling	1	0
3	SGTC_241	5hr heat shock (37°C) + cantharidin	41.40	155.4 µM	4.18E-167	cell wall signaling	2	0
4	SGTC_2009	4012297	40.30	12.1 µM	5.92E-158	cell wall signaling	1	0
5	SGTC_2912	7964298	37.60	10.9 µM	3.34E-138	cell wall	1	0
6	SGTC_2007	4011539	37.10	71.8 µM	2.47E-134	cell wall signaling	1	2.00E-301
7	SGTC_137	0438-0332	36.90	85.4 µM	3.42E-133	ubiquinone biosynthesis & proteasome	2	7.67E-299
8	SGTC_138	0850-0259	33.40	40.9 µM	4.17E-109	cell wall	1	2.61E-244
9	SGTC_663	1486-0733	31.00	13.9 µM	1.22E-94	cell wall signaling	2	1.44E-211
10	SGTC_142	0335-0849	29.30	62.7 µM	1.52E-84	ubiquinone biosynthesis & proteasome	2	1.02E-188
11	SGTC_408	cantharidin	28.90	100.0 µM	3.00E-82	cell wall signaling	1	1.59E-183
12	SGTC_905	2-aminofluorene	28.40	215.0 µM	2.04E-79	cell wall signaling	1	4.10E-177
13	SGTC_71	0417-1750	25.20	47.8 µM	2.11E-63	Golgi	1	6.98E-141
14	SGTC_825	1988-2224	24.80	89.6 µM	3.81E-61	cell wall signaling	1	8.82E-136
15	SGTC_2028	5153890	23.30	36.4 µM	2.02E-54	cell wall signaling	1	1.42E-120
16	SGTC_1874	staurosporine	20.80	7.6 µM	1.21E-43	cell wall signaling	1	3.26E-96
17	SGTC_2003	4023581	20.20	200.0 µM	1.96E-41	cell wall signaling	1	3.23E-91
18	SGTC_360	0957-0399	20.00	1.0 µM	1.79E-40	cell wall	2	4.73E-89
19	SGTC_2149	5554786	19.70	194.8 µM	2.95E-39	cell wall signaling	1	2.68E-86
20	SGTC_354	2783-3500	19.40	4.9 µM	2.61E-38	cell wall	1	3.67E-84

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.777	0	YHR030C	SLT2	hom	Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
0.398	1.88E-127	YLR332W	MID2	hom	O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.387	4.07E-120	YPR165W	RHO1	het	GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.364	1.00E-105	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.328	9.42E-85	YPL089C	RLM1	hom	MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
0.322	5.70E-82	YLR331C_d	JIP3_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.311	5.18E-76	YGR196C	FYV8	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.284	4.12E-63	YNL322C	KRE1	hom	Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.269	1.60E-56	YNL084C	END3	hom	EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
0.266	2.89E-55	YOR008C	SLG1	hom	Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
0.260	5.39E-53	YHR209W	CRG1	hom	S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.259	1.02E-52	YLL043W	FPS1	hom	Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.257	7.36E-52	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.249	8.35E-49	YBR260C	RGD1	hom	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.240	2.32E-45	YNL079C	TPM1	hom	Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication