YJL097W / PHS1

Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL097W / PHS1

Click on Significant Values for Screen Details ID:SGTC_335|Compound:4077-0256|FD-Score:-2.82|P-value:9.70E-4 ID:SGTC_336|Compound:4111-0029|FD-Score:-2.82|P-value:9.78E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:3.45|P-value:4.05E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:2.78|P-value:7.04E-4 ID:SGTC_500|Compound:h-89|FD-Score:-4.21|P-value:1.64E-6 ID:SGTC_523|Compound:1934-0222|FD-Score:2.83|P-value:5.77E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:-3.90|P-value:8.40E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:-3.24|P-value:1.82E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:-3.71|P-value:2.08E-5 ID:SGTC_926|Compound:2268-1401|FD-Score:3.16|P-value:1.48E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:3.63|P-value:1.67E-5 ID:SGTC_987|Compound:1497-1013|FD-Score:4.34|P-value:3.94E-7 ID:SGTC_988|Compound:1497-1057|FD-Score:3.05|P-value:2.39E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:2.88|P-value:4.83E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.01|P-value:2.80E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.45|P-value:7.13E-5 ID:SGTC_1051|Compound:3253-3903|FD-Score:-3.87|P-value:9.61E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:-5.85|P-value:4.11E-11 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.72|P-value:1.99E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.43|P-value:7.94E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.23|P-value:1.10E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:2.91|P-value:4.22E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-3.21|P-value:2.03E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.89|P-value:4.62E-6 ID:SGTC_1807|Compound:st050092|FD-Score:3.25|P-value:1.02E-4 ID:SGTC_1816|Compound:st051957|FD-Score:-3.37|P-value:1.04E-4 ID:SGTC_1838|Compound:st055369|FD-Score:-2.91|P-value:6.85E-4 ID:SGTC_2108|Compound:5365811|FD-Score:-3.87|P-value:9.50E-6 ID:SGTC_2304|Compound:7443016|FD-Score:-3.18|P-value:2.33E-4 ID:SGTC_2307|Compound:7538926|FD-Score:4.70|P-value:4.46E-8 ID:SGTC_2311|Compound:7694451|FD-Score:4.12|P-value:1.34E-6 ID:SGTC_2411|Compound:st077133|FD-Score:3.36|P-value:6.21E-5 ID:SGTC_2412|Compound:st077135|FD-Score:3.35|P-value:6.35E-5 ID:SGTC_2413|Compound:inermin|FD-Score:2.87|P-value:4.91E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.33|P-value:6.83E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.82|P-value:6.44E-6 ID:SGTC_2424|Compound:5537928|FD-Score:4.20|P-value:8.58E-7 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-5.31|P-value:1.90E-9 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.05|P-value:2.36E-4 ID:SGTC_2570|Compound:licareol|FD-Score:3.61|P-value:1.89E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.60|P-value:1.92E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.87|P-value:5.06E-6 ID:SGTC_2731|Compound:amoxapine|FD-Score:2.91|P-value:4.15E-4 ID:SGTC_2793|Compound:7325131|FD-Score:4.75|P-value:3.23E-8 ID:SGTC_2796|Compound:7949634|FD-Score:4.05|P-value:1.97E-6 ID:SGTC_2881|Compound:9058953|FD-Score:3.10|P-value:1.88E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.13|P-value:1.65E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-4.61|P-value:1.65E-7 ID:SGTC_3115|Compound:9123671|FD-Score:-4.04|P-value:4.05E-6 ID:SGTC_3118|Compound:9124250|FD-Score:2.82|P-value:5.96E-4 ID:SGTC_3120|Compound:9124077|FD-Score:-3.49|P-value:5.95E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-2.82|P-value:9.70E-4 ID:SGTC_336|Compound:4111-0029|FD-Score:-2.82|P-value:9.78E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:3.45|P-value:4.05E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:2.78|P-value:7.04E-4 ID:SGTC_500|Compound:h-89|FD-Score:-4.21|P-value:1.64E-6 ID:SGTC_523|Compound:1934-0222|FD-Score:2.83|P-value:5.77E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:-3.90|P-value:8.40E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:-3.24|P-value:1.82E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:-3.71|P-value:2.08E-5 ID:SGTC_926|Compound:2268-1401|FD-Score:3.16|P-value:1.48E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:3.63|P-value:1.67E-5 ID:SGTC_987|Compound:1497-1013|FD-Score:4.34|P-value:3.94E-7 ID:SGTC_988|Compound:1497-1057|FD-Score:3.05|P-value:2.39E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:2.88|P-value:4.83E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.01|P-value:2.80E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.45|P-value:7.13E-5 ID:SGTC_1051|Compound:3253-3903|FD-Score:-3.87|P-value:9.61E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:-5.85|P-value:4.11E-11 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.72|P-value:1.99E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.43|P-value:7.94E-5 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.23|P-value:1.10E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:2.91|P-value:4.22E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-3.21|P-value:2.03E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.89|P-value:4.62E-6 ID:SGTC_1807|Compound:st050092|FD-Score:3.25|P-value:1.02E-4 ID:SGTC_1816|Compound:st051957|FD-Score:-3.37|P-value:1.04E-4 ID:SGTC_1838|Compound:st055369|FD-Score:-2.91|P-value:6.85E-4 ID:SGTC_2108|Compound:5365811|FD-Score:-3.87|P-value:9.50E-6 ID:SGTC_2304|Compound:7443016|FD-Score:-3.18|P-value:2.33E-4 ID:SGTC_2307|Compound:7538926|FD-Score:4.70|P-value:4.46E-8 ID:SGTC_2311|Compound:7694451|FD-Score:4.12|P-value:1.34E-6 ID:SGTC_2411|Compound:st077133|FD-Score:3.36|P-value:6.21E-5 ID:SGTC_2412|Compound:st077135|FD-Score:3.35|P-value:6.35E-5 ID:SGTC_2413|Compound:inermin|FD-Score:2.87|P-value:4.91E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.33|P-value:6.83E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.82|P-value:6.44E-6 ID:SGTC_2424|Compound:5537928|FD-Score:4.20|P-value:8.58E-7 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-5.31|P-value:1.90E-9 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.05|P-value:2.36E-4 ID:SGTC_2570|Compound:licareol|FD-Score:3.61|P-value:1.89E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.60|P-value:1.92E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.87|P-value:5.06E-6 ID:SGTC_2731|Compound:amoxapine|FD-Score:2.91|P-value:4.15E-4 ID:SGTC_2793|Compound:7325131|FD-Score:4.75|P-value:3.23E-8 ID:SGTC_2796|Compound:7949634|FD-Score:4.05|P-value:1.97E-6 ID:SGTC_2881|Compound:9058953|FD-Score:3.10|P-value:1.88E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.13|P-value:1.65E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-4.61|P-value:1.65E-7 ID:SGTC_3115|Compound:9123671|FD-Score:-4.04|P-value:4.05E-6 ID:SGTC_3118|Compound:9124250|FD-Score:2.82|P-value:5.96E-4 ID:SGTC_3120|Compound:9124077|FD-Score:-3.49|P-value:5.95E-5

Top fitness defect scores for YJL097W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2793 7325131 4.75 81.8 µM 3.23E-8 3 1.01E-6 0.07
2 SGTC_2307 7538926 4.70 6.3 µM 4.46E-8 14 1.30E-6 0.14
3 SGTC_987 1497-1013 4.34 404.0 µM 3.94E-7 1 7.27E-6 0.42
4 SGTC_2424 5537928 4.20 59.2 µM 8.58E-7 8 1.34E-5 0.23
5 SGTC_2311 7694451 4.12 200.0 µM 1.34E-6 7 1.91E-5 0.10
6 SGTC_2796 7949634 4.05 45.5 µM 1.97E-6 5 2.59E-5 0.11
7 SGTC_1748 st037455 3.89 53.6 µM 4.62E-6 9 5.08E-5 0.35
8 SGTC_2621 lithochol-11-enic acid 3.87 100.0 µM 5.06E-6 7 5.46E-5 0.05
9 SGTC_2422 chlorphenesin carbamate 3.82 384.2 µM 6.44E-6 6 6.60E-5 0.13
10 SGTC_938 3126-1755 3.63 342.0 µM 1.67E-5 3 1.40E-4 0.13
11 SGTC_2570 licareol 3.61 100.0 µM 1.89E-5 6 1.54E-4 0.02
12 SGTC_2574 2',4'-dihydroxychalcone 3.60 14.6 µM 1.92E-5 15 1.57E-4 0.03
13 SGTC_429 1112-0049 3.45 25.5 µM 4.05E-5 DNA damage response 14 2.83E-4 0.06
14 SGTC_2411 st077133 3.36 39.9 µM 6.21E-5 RNA pol III & RNase P/MRP 26 3.96E-4 0.07
15 SGTC_2412 st077135 3.35 33.4 µM 6.35E-5 9 4.03E-4 0.11
16 SGTC_2415 st077333 3.33 57.7 µM 6.83E-5 23 4.27E-4 0.08
17 SGTC_1807 st050092 3.25 58.9 µM 1.02E-4 60S ribosome export 11 5.87E-4 0.28
18 SGTC_1246 0422-0085 3.23 105.0 µM 1.10E-4 18 6.21E-4 0.06
19 SGTC_926 2268-1401 3.16 59.4 µM 1.48E-4 TSC3-RPN4 15 7.90E-4 0.00
20 SGTC_2953 9074162 3.13 66.5 µM 1.65E-4 13 8.59E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 2.97E-18 YGR127W_p YGR127W_p hom Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
0.111 1.33E-10 YBR043C QDR3 hom Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin
0.102 3.18E-9 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.098 1.11E-8 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.096 2.70E-8 YBR083W TEC1 hom Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member
0.095 4.08E-8 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.093 7.74E-8 YLR286C CTS1 hom Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
0.091 1.42E-7 YDR141C DOP1 het Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
0.090 1.80E-7 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
0.087 5.09E-7 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.084 1.29E-6 YMR283C RIT1 hom 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.083 1.68E-6 YGR169C PUS6 hom tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability
0.082 1.74E-6 YKL222C YKL222C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
0.080 3.67E-6 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.079 5.14E-6 YKL162C-A_d YKL162C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data