YJL101C / GSH1

Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL101C / GSH1

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:2.91|P-value:9.54E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.06|P-value:5.51E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.30|P-value:2.17E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:2.99|P-value:7.08E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:9.73|P-value:1.79E-25 ID:SGTC_163|Compound:1327-0036|FD-Score:4.92|P-value:7.74E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:22.10|P-value:2.68E-123 ID:SGTC_184|Compound:1494-0393|FD-Score:9.21|P-value:4.70E-23 ID:SGTC_367|Compound:0335-0881|FD-Score:3.08|P-value:5.04E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:2.92|P-value:9.24E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.82|P-value:2.29E-5 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:3.01|P-value:6.72E-4 ID:SGTC_398|Compound:bronopol|FD-Score:7.53|P-value:4.97E-16 ID:SGTC_523|Compound:1934-0222|FD-Score:3.94|P-value:1.32E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:3.15|P-value:3.90E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:5.00|P-value:4.90E-8 ID:SGTC_778|Compound:3456-2434|FD-Score:4.41|P-value:1.30E-6 ID:SGTC_824|Compound:1304-0008|FD-Score:3.69|P-value:4.09E-5 ID:SGTC_842|Compound:0271-0008|FD-Score:3.00|P-value:6.78E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.67|P-value:4.51E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:4.02|P-value:8.90E-6 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.77|P-value:2.98E-5 ID:SGTC_1141|Compound:3253-0389|FD-Score:5.94|P-value:1.21E-10 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.22|P-value:2.96E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.48|P-value:1.06E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:4.22|P-value:3.39E-6 ID:SGTC_1371|Compound:2421-0008|FD-Score:-3.52|P-value:9.13E-5 ID:SGTC_1374|Compound:2889-5571|FD-Score:-4.75|P-value:2.15E-7 ID:SGTC_1501|Compound:4491-0275|FD-Score:2.98|P-value:7.33E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:-2.95|P-value:8.41E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.42|P-value:1.32E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-4.09|P-value:6.68E-6 ID:SGTC_1700|Compound:st030840|FD-Score:4.58|P-value:5.32E-7 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.48|P-value:1.02E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.54|P-value:7.97E-5 ID:SGTC_2126|Compound:5251174|FD-Score:3.01|P-value:6.70E-4 ID:SGTC_2174|Compound:5790901|FD-Score:2.93|P-value:8.96E-4 ID:SGTC_2200|Compound:6600604|FD-Score:5.86|P-value:2.11E-10 ID:SGTC_2218|Compound:6965428|FD-Score:3.25|P-value:2.64E-4 ID:SGTC_2306|Compound:7482397|FD-Score:2.99|P-value:7.11E-4 ID:SGTC_2348|Compound:9022543|FD-Score:-4.55|P-value:6.39E-7 ID:SGTC_2405|Compound:ticlatone|FD-Score:11.40|P-value:3.18E-34 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:6.57|P-value:1.22E-12 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.94|P-value:1.32E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.02|P-value:6.42E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.94|P-value:6.88E-8 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.88|P-value:1.76E-5 ID:SGTC_2828|Compound:7998861|FD-Score:2.98|P-value:7.50E-4 ID:SGTC_2844|Compound:9010103|FD-Score:-2.96|P-value:8.13E-4 ID:SGTC_2881|Compound:9058953|FD-Score:-3.44|P-value:1.27E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.29|P-value:2.23E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.07|P-value:5.37E-4 ID:SGTC_2996|Compound:9066192|FD-Score:3.52|P-value:8.82E-5 ID:SGTC_3028|Compound:9087778|FD-Score:-3.84|P-value:2.23E-5 ID:SGTC_3037|Compound:9091026|FD-Score:5.66|P-value:7.84E-10 ID:SGTC_3038|Compound:9091290|FD-Score:3.07|P-value:5.26E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:4.02|P-value:8.85E-6 ID:SGTC_3162|Compound:9099653|FD-Score:4.14|P-value:5.12E-6 ID:SGTC_3197|Compound:9113331|FD-Score:2.99|P-value:7.11E-4 ID:SGTC_3210|Compound:9128157|FD-Score:7.00|P-value:4.37E-14 ID:SGTC_3292|Compound:9120825|FD-Score:3.31|P-value:2.04E-4 ID:SGTC_3293|Compound:9120939|FD-Score:3.67|P-value:4.57E-5 ID:SGTC_23|Compound:0213-0011|FD-Score:2.91|P-value:9.54E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.06|P-value:5.51E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.30|P-value:2.17E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:2.99|P-value:7.08E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:9.73|P-value:1.79E-25 ID:SGTC_163|Compound:1327-0036|FD-Score:4.92|P-value:7.74E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:22.10|P-value:2.68E-123 ID:SGTC_184|Compound:1494-0393|FD-Score:9.21|P-value:4.70E-23 ID:SGTC_367|Compound:0335-0881|FD-Score:3.08|P-value:5.04E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:2.92|P-value:9.24E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.82|P-value:2.29E-5 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:3.01|P-value:6.72E-4 ID:SGTC_398|Compound:bronopol|FD-Score:7.53|P-value:4.97E-16 ID:SGTC_523|Compound:1934-0222|FD-Score:3.94|P-value:1.32E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:3.15|P-value:3.90E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:5.00|P-value:4.90E-8 ID:SGTC_778|Compound:3456-2434|FD-Score:4.41|P-value:1.30E-6 ID:SGTC_824|Compound:1304-0008|FD-Score:3.69|P-value:4.09E-5 ID:SGTC_842|Compound:0271-0008|FD-Score:3.00|P-value:6.78E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.67|P-value:4.51E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:4.02|P-value:8.90E-6 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.77|P-value:2.98E-5 ID:SGTC_1141|Compound:3253-0389|FD-Score:5.94|P-value:1.21E-10 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.22|P-value:2.96E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.48|P-value:1.06E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:4.22|P-value:3.39E-6 ID:SGTC_1371|Compound:2421-0008|FD-Score:-3.52|P-value:9.13E-5 ID:SGTC_1374|Compound:2889-5571|FD-Score:-4.75|P-value:2.15E-7 ID:SGTC_1501|Compound:4491-0275|FD-Score:2.98|P-value:7.33E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:-2.95|P-value:8.41E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.42|P-value:1.32E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-4.09|P-value:6.68E-6 ID:SGTC_1700|Compound:st030840|FD-Score:4.58|P-value:5.32E-7 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.48|P-value:1.02E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.54|P-value:7.97E-5 ID:SGTC_2126|Compound:5251174|FD-Score:3.01|P-value:6.70E-4 ID:SGTC_2174|Compound:5790901|FD-Score:2.93|P-value:8.96E-4 ID:SGTC_2200|Compound:6600604|FD-Score:5.86|P-value:2.11E-10 ID:SGTC_2218|Compound:6965428|FD-Score:3.25|P-value:2.64E-4 ID:SGTC_2306|Compound:7482397|FD-Score:2.99|P-value:7.11E-4 ID:SGTC_2348|Compound:9022543|FD-Score:-4.55|P-value:6.39E-7 ID:SGTC_2405|Compound:ticlatone|FD-Score:11.40|P-value:3.18E-34 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:6.57|P-value:1.22E-12 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.94|P-value:1.32E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.02|P-value:6.42E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.94|P-value:6.88E-8 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.88|P-value:1.76E-5 ID:SGTC_2828|Compound:7998861|FD-Score:2.98|P-value:7.50E-4 ID:SGTC_2844|Compound:9010103|FD-Score:-2.96|P-value:8.13E-4 ID:SGTC_2881|Compound:9058953|FD-Score:-3.44|P-value:1.27E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.29|P-value:2.23E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.07|P-value:5.37E-4 ID:SGTC_2996|Compound:9066192|FD-Score:3.52|P-value:8.82E-5 ID:SGTC_3028|Compound:9087778|FD-Score:-3.84|P-value:2.23E-5 ID:SGTC_3037|Compound:9091026|FD-Score:5.66|P-value:7.84E-10 ID:SGTC_3038|Compound:9091290|FD-Score:3.07|P-value:5.26E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:4.02|P-value:8.85E-6 ID:SGTC_3162|Compound:9099653|FD-Score:4.14|P-value:5.12E-6 ID:SGTC_3197|Compound:9113331|FD-Score:2.99|P-value:7.11E-4 ID:SGTC_3210|Compound:9128157|FD-Score:7.00|P-value:4.37E-14 ID:SGTC_3292|Compound:9120825|FD-Score:3.31|P-value:2.04E-4 ID:SGTC_3293|Compound:9120939|FD-Score:3.67|P-value:4.57E-5

Top fitness defect scores for YJL101C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_168 3970-0862 22.10 530.5 µM 2.68E-123 1 6.39E-109
2 SGTC_2405 ticlatone 11.40 3.5 µM 3.18E-34 8 2.21E-30
3 SGTC_150 3970-0969 9.73 77.2 µM 1.79E-25 3 1.16E-22
4 SGTC_184 1494-0393 9.21 24.7 µM 4.70E-23 8 1.58E-20
5 SGTC_398 bronopol 7.53 33.3 µM 4.97E-16 RNA processing & uracil transport 5 2.49E-14
6 SGTC_3210 9128157 7.00 49.5 µM 4.37E-14 9 1.30E-12
7 SGTC_2580 diallyl disulphide 6.57 81.4 µM 1.22E-12 4 2.45E-11
8 SGTC_1141 3253-0389 5.94 52.6 µM 1.21E-10 1 1.42E-9
9 SGTC_2200 6600604 5.86 21.9 µM 2.11E-10 tubulin folding & SWR complex 31 2.33E-9
10 SGTC_3037 9091026 5.66 49.5 µM 7.84E-10 NEO1-PIK1 57 7.42E-9
11 SGTC_695 3505-6186 5.00 243.0 µM 4.90E-8 10 2.87E-7
12 SGTC_2751 anetholtrithion 4.94 17.2 µM 6.88E-8 12 3.87E-7
13 SGTC_163 1327-0036 4.92 7.5 µM 7.74E-8 65 4.30E-7
14 SGTC_1700 st030840 4.58 7.9 µM 5.32E-7 PDR1 17 2.37E-6
15 SGTC_778 3456-2434 4.41 196.0 µM 1.30E-6 mitochondrial response to ROS 62 5.23E-6
16 SGTC_1275 0867-0008 4.22 21.1 µM 3.39E-6 10 1.22E-5
17 SGTC_3162 9099653 4.14 49.5 µM 5.12E-6 3 1.76E-5
18 SGTC_3059 1327-0034 4.02 3.0 µM 8.85E-6 51 2.85E-5
19 SGTC_984 1492-0255 4.02 2.9 µM 8.90E-6 SWF1 & branched chain AA biosynthesis 26 2.87E-5
20 SGTC_523 1934-0222 3.94 13.7 µM 1.32E-5 31 4.06E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.307 3.41E-74 YOL049W GSH2 hom Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
0.278 2.82E-60 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.247 1.09E-47 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.233 2.57E-42 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.231 1.07E-41 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.223 6.56E-39 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.222 1.17E-38 YJL054W TIM54 het Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.220 6.63E-38 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.214 7.86E-36 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.201 6.40E-32 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.200 1.41E-31 YDR002W YRB1 het Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
0.200 2.26E-31 YJR074W MOG1 hom Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
0.195 4.24E-30 YKL028W TFA1 het TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
0.194 1.26E-29 YML073C RPL6A hom Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
0.179 2.18E-25 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M