YJL116C / NCA3

Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL116C / NCA3

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.07|P-value:4.90E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.28|P-value:2.14E-4 ID:SGTC_379|Compound:3970-0972|FD-Score:-3.27|P-value:2.20E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:3.50|P-value:8.55E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:3.73|P-value:3.16E-5 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:4.23|P-value:2.78E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:-3.43|P-value:1.14E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.45|P-value:1.06E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-2.93|P-value:8.27E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.09|P-value:4.64E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.33|P-value:1.76E-4 ID:SGTC_1729|Compound:st035615|FD-Score:2.95|P-value:7.67E-4 ID:SGTC_1730|Compound:st033808|FD-Score:-3.88|P-value:1.59E-5 ID:SGTC_1778|Compound:st046819|FD-Score:4.04|P-value:7.16E-6 ID:SGTC_1788|Compound:fluconazole|FD-Score:8.03|P-value:3.56E-18 ID:SGTC_1792|Compound:st045773|FD-Score:-3.09|P-value:4.63E-4 ID:SGTC_1845|Compound:st056753|FD-Score:3.23|P-value:2.62E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-3.39|P-value:1.37E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-2.93|P-value:8.40E-4 ID:SGTC_2020|Compound:4017018|FD-Score:3.27|P-value:2.24E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.13|P-value:3.93E-4 ID:SGTC_2343|Compound:7990716|FD-Score:3.05|P-value:5.34E-4 ID:SGTC_2346|Compound:9031566|FD-Score:-2.97|P-value:7.20E-4 ID:SGTC_2457|Compound:5731572|FD-Score:3.36|P-value:1.60E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.25|P-value:2.45E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.06|P-value:5.07E-4 ID:SGTC_2657|Compound:nystatin|FD-Score:3.69|P-value:3.86E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:4.01|P-value:8.61E-6 ID:SGTC_2846|Compound:9008642|FD-Score:5.21|P-value:1.12E-8 ID:SGTC_3049|Compound:9092223|FD-Score:-4.58|P-value:4.54E-7 ID:SGTC_3182|Compound:9108849|FD-Score:4.08|P-value:5.96E-6 ID:SGTC_3333|Compound:9141820|FD-Score:3.81|P-value:2.24E-5 ID:SGTC_6|Compound:0844-0013|FD-Score:3.07|P-value:4.90E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.28|P-value:2.14E-4 ID:SGTC_379|Compound:3970-0972|FD-Score:-3.27|P-value:2.20E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:3.50|P-value:8.55E-5 ID:SGTC_864|Compound:0469-0573|FD-Score:3.73|P-value:3.16E-5 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:4.23|P-value:2.78E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:-3.43|P-value:1.14E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.45|P-value:1.06E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-2.93|P-value:8.27E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-3.09|P-value:4.64E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.33|P-value:1.76E-4 ID:SGTC_1729|Compound:st035615|FD-Score:2.95|P-value:7.67E-4 ID:SGTC_1730|Compound:st033808|FD-Score:-3.88|P-value:1.59E-5 ID:SGTC_1778|Compound:st046819|FD-Score:4.04|P-value:7.16E-6 ID:SGTC_1788|Compound:fluconazole|FD-Score:8.03|P-value:3.56E-18 ID:SGTC_1792|Compound:st045773|FD-Score:-3.09|P-value:4.63E-4 ID:SGTC_1845|Compound:st056753|FD-Score:3.23|P-value:2.62E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-3.39|P-value:1.37E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-2.93|P-value:8.40E-4 ID:SGTC_2020|Compound:4017018|FD-Score:3.27|P-value:2.24E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.13|P-value:3.93E-4 ID:SGTC_2343|Compound:7990716|FD-Score:3.05|P-value:5.34E-4 ID:SGTC_2346|Compound:9031566|FD-Score:-2.97|P-value:7.20E-4 ID:SGTC_2457|Compound:5731572|FD-Score:3.36|P-value:1.60E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.25|P-value:2.45E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.06|P-value:5.07E-4 ID:SGTC_2657|Compound:nystatin|FD-Score:3.69|P-value:3.86E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:4.01|P-value:8.61E-6 ID:SGTC_2846|Compound:9008642|FD-Score:5.21|P-value:1.12E-8 ID:SGTC_3049|Compound:9092223|FD-Score:-4.58|P-value:4.54E-7 ID:SGTC_3182|Compound:9108849|FD-Score:4.08|P-value:5.96E-6 ID:SGTC_3333|Compound:9141820|FD-Score:3.81|P-value:2.24E-5

Top fitness defect scores for YJL116C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1788 fluconazole 8.03 20.0 µM 3.56E-18 azole & statin 19 4.93E-16
2 SGTC_2846 9008642 5.21 19.5 µM 1.12E-8 6 9.30E-8
3 SGTC_1102 cgs 12066b 4.23 26.1 µM 2.78E-6 endomembrane recycling 6 1.14E-5
4 SGTC_3182 9108849 4.08 49.5 µM 5.96E-6 RPP1 & pyrimidine depletion 11 2.23E-5
5 SGTC_1778 st046819 4.04 58.9 µM 7.16E-6 22 2.62E-5
6 SGTC_2777 climbazole 4.01 1.5 µM 8.61E-6 iron homeostasis 48 3.07E-5
7 SGTC_3333 9141820 3.81 12.1 µM 2.24E-5 16 7.09E-5
8 SGTC_864 0469-0573 3.73 172.0 µM 3.16E-5 26 9.59E-5
9 SGTC_2657 nystatin 3.69 74.4 µM 3.86E-5 19 1.14E-4
10 SGTC_849 0386-0493 3.50 241.0 µM 8.55E-5 32 2.29E-4
11 SGTC_2457 5731572 3.36 11.7 µM 1.60E-4 27 3.96E-4
12 SGTC_1719 st032227 3.33 49.2 µM 1.76E-4 45 4.32E-4
13 SGTC_2020 4017018 3.27 200.0 µM 2.24E-4 47 5.34E-4
14 SGTC_1845 st056753 3.23 3.0 µM 2.62E-4 TSC3-RPN4 64 6.12E-4
15 SGTC_2341 7987486 3.13 176.7 µM 3.93E-4 48 8.73E-4
16 SGTC_6 0844-0013 3.07 19.8 µM 4.90E-4 196 0.00106
17 SGTC_2563 tomatine 3.06 8.5 µM 5.07E-4 103 0.00109
18 SGTC_2343 7990716 3.05 14.8 µM 5.34E-4 49 0.00114
19 SGTC_1729 st035615 2.95 46.0 µM 7.67E-4 82 0.00157
20 SGTC_3032 9089427 2.86 49.5 µM 0.00110 60S ribosome export 57 0.00215

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.160 1.18E-20 YDL118W_d YDL118W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
0.146 2.14E-17 YMR063W RIM9 hom Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
0.136 3.17E-15 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.130 5.12E-14 YLR336C SGD1 het Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
0.127 1.75E-13 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.121 2.15E-12 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.116 1.91E-11 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.115 2.48E-11 YIL088C AVT7 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.112 8.52E-11 YDL063C SYO1 hom Transport adaptor or symportin; facilitates synchronized nuclear coimport of the two 5S-rRNA binding proteins Rpl5p and Rpl11p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.111 1.37E-10 YGL061C DUO1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.108 2.96E-10 YEL055C POL5 het DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
0.105 9.64E-10 YPR009W SUT2 hom Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p
0.104 1.31E-9 YOL034W SMC5 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
0.099 8.37E-9 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.099 8.49E-9 YPL170W DAP1 hom Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis