YJL117W / PHO86

Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL117W / PHO86

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:5.26|P-value:7.09E-7 ID:SGTC_18|Compound:0111-0177|FD-Score:5.19|P-value:9.83E-7 ID:SGTC_24|Compound:0417-1663|FD-Score:3.58|P-value:4.61E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-13.30|P-value:1.07E-32 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.72|P-value:6.17E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.12|P-value:1.68E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.80|P-value:4.89E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.99|P-value:2.60E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.96|P-value:2.86E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:-6.36|P-value:1.12E-8 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.96|P-value:2.88E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:4.20|P-value:5.54E-5 ID:SGTC_775|Compound:4577-0580|FD-Score:-3.79|P-value:5.00E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-6.44|P-value:7.42E-9 ID:SGTC_862|Compound:0452-0056|FD-Score:-3.76|P-value:5.44E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:-3.58|P-value:9.52E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:-4.24|P-value:1.09E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:-3.77|P-value:5.34E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:-3.74|P-value:5.89E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:3.77|P-value:2.46E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.72|P-value:2.88E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:4.01|P-value:1.06E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:4.45|P-value:2.14E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.49|P-value:6.04E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-3.85|P-value:4.19E-4 ID:SGTC_1633|Compound:st005664|FD-Score:5.07|P-value:1.64E-6 ID:SGTC_1682|Compound:st012968|FD-Score:-4.92|P-value:8.42E-6 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.20|P-value:2.65E-6 ID:SGTC_1937|Compound:st074714|FD-Score:3.97|P-value:1.26E-4 ID:SGTC_2023|Compound:5144124|FD-Score:5.08|P-value:1.60E-6 ID:SGTC_2026|Compound:5148700|FD-Score:3.43|P-value:7.33E-4 ID:SGTC_2106|Compound:5357045|FD-Score:3.50|P-value:6.01E-4 ID:SGTC_2108|Compound:5365811|FD-Score:3.73|P-value:2.87E-4 ID:SGTC_2253|Compound:7938677|FD-Score:-3.70|P-value:6.65E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.63|P-value:8.33E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.65|P-value:3.66E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.81|P-value:4.71E-4 ID:SGTC_2452|Compound:5863136|FD-Score:5.98|P-value:2.37E-8 ID:SGTC_2479|Compound:5617353|FD-Score:3.67|P-value:3.46E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:4.88|P-value:3.70E-6 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.47|P-value:6.54E-4 ID:SGTC_2523|Compound:spermine|FD-Score:4.25|P-value:4.50E-5 ID:SGTC_2681|Compound:menadione|FD-Score:4.63|P-value:1.04E-5 ID:SGTC_2712|Compound:st079126|FD-Score:4.11|P-value:7.62E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:-3.58|P-value:9.60E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-4.09|P-value:1.84E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-3.64|P-value:7.88E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:-4.10|P-value:1.81E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.49|P-value:4.46E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:-5.46|P-value:8.58E-7 ID:SGTC_2900|Compound:5328528|FD-Score:5.43|P-value:3.39E-7 ID:SGTC_2938|Compound:9059746|FD-Score:4.09|P-value:8.06E-5 ID:SGTC_3131|Compound:9127911|FD-Score:-3.89|P-value:3.68E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.29|P-value:9.25E-5 ID:SGTC_11|Compound:0438-0307|FD-Score:5.26|P-value:7.09E-7 ID:SGTC_18|Compound:0111-0177|FD-Score:5.19|P-value:9.83E-7 ID:SGTC_24|Compound:0417-1663|FD-Score:3.58|P-value:4.61E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-13.30|P-value:1.07E-32 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.72|P-value:6.17E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.12|P-value:1.68E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.80|P-value:4.89E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.99|P-value:2.60E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.96|P-value:2.86E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:-6.36|P-value:1.12E-8 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.96|P-value:2.88E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:4.20|P-value:5.54E-5 ID:SGTC_775|Compound:4577-0580|FD-Score:-3.79|P-value:5.00E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-6.44|P-value:7.42E-9 ID:SGTC_862|Compound:0452-0056|FD-Score:-3.76|P-value:5.44E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:-3.58|P-value:9.52E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:-4.24|P-value:1.09E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:-3.77|P-value:5.34E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:-3.74|P-value:5.89E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:3.77|P-value:2.46E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.72|P-value:2.88E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:4.01|P-value:1.06E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:4.45|P-value:2.14E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.49|P-value:6.04E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-3.85|P-value:4.19E-4 ID:SGTC_1633|Compound:st005664|FD-Score:5.07|P-value:1.64E-6 ID:SGTC_1682|Compound:st012968|FD-Score:-4.92|P-value:8.42E-6 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.20|P-value:2.65E-6 ID:SGTC_1937|Compound:st074714|FD-Score:3.97|P-value:1.26E-4 ID:SGTC_2023|Compound:5144124|FD-Score:5.08|P-value:1.60E-6 ID:SGTC_2026|Compound:5148700|FD-Score:3.43|P-value:7.33E-4 ID:SGTC_2106|Compound:5357045|FD-Score:3.50|P-value:6.01E-4 ID:SGTC_2108|Compound:5365811|FD-Score:3.73|P-value:2.87E-4 ID:SGTC_2253|Compound:7938677|FD-Score:-3.70|P-value:6.65E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.63|P-value:8.33E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.65|P-value:3.66E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.81|P-value:4.71E-4 ID:SGTC_2452|Compound:5863136|FD-Score:5.98|P-value:2.37E-8 ID:SGTC_2479|Compound:5617353|FD-Score:3.67|P-value:3.46E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:4.88|P-value:3.70E-6 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.47|P-value:6.54E-4 ID:SGTC_2523|Compound:spermine|FD-Score:4.25|P-value:4.50E-5 ID:SGTC_2681|Compound:menadione|FD-Score:4.63|P-value:1.04E-5 ID:SGTC_2712|Compound:st079126|FD-Score:4.11|P-value:7.62E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:-3.58|P-value:9.60E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-4.09|P-value:1.84E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-3.64|P-value:7.88E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:-4.10|P-value:1.81E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.49|P-value:4.46E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:-5.46|P-value:8.58E-7 ID:SGTC_2900|Compound:5328528|FD-Score:5.43|P-value:3.39E-7 ID:SGTC_2938|Compound:9059746|FD-Score:4.09|P-value:8.06E-5 ID:SGTC_3131|Compound:9127911|FD-Score:-3.89|P-value:3.68E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.29|P-value:9.25E-5

Top fitness defect scores for YJL117W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2452 5863136 5.98 48.4 µM 2.37E-8 4 1.15E-9
2 SGTC_2900 5328528 5.43 85.3 µM 3.39E-7 Golgi 9 2.89E-8
3 SGTC_11 0438-0307 5.26 1.3 µM 7.09E-7 2 7.07E-8
4 SGTC_18 0111-0177 5.19 500.0 nM 9.83E-7 30 1.05E-7
5 SGTC_2023 5144124 5.08 124.0 µM 1.60E-6 9 1.89E-7
6 SGTC_1633 st005664 5.07 67.9 µM 1.64E-6 13 1.95E-7
7 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 4.88 54.6 µM 3.70E-6 RPP1 & pyrimidine depletion 15 5.22E-7
8 SGTC_2681 menadione 4.63 3.2 µM 1.04E-5 superoxide 20 1.82E-6
9 SGTC_1465 k213-0056 4.45 144.0 µM 2.14E-5 DNA intercalators 14 4.33E-6
10 SGTC_2523 spermine 4.25 47.7 µM 4.50E-5 calcium & mitochondrial duress 26 1.06E-5
11 SGTC_706 4262-0057 4.20 46.9 µM 5.54E-5 fatty acid desaturase (OLE1) 28 1.36E-5
12 SGTC_2712 st079126 4.11 47.7 µM 7.62E-5 11 1.99E-5
13 SGTC_2938 9059746 4.09 27.3 µM 8.06E-5 fatty acid desaturase (OLE1) 38 2.13E-5
14 SGTC_1286 0929-0070 4.01 131.0 µM 1.06E-4 17 2.97E-5
15 SGTC_1937 st074714 3.97 47.2 µM 1.26E-4 41 3.63E-5
16 SGTC_1060 3448-7350 3.77 82.0 µM 2.46E-4 Golgi 39 8.12E-5
17 SGTC_2108 5365811 3.73 200.0 µM 2.87E-4 20 9.76E-5
18 SGTC_1274 0865-0114 3.72 173.0 µM 2.88E-4 29 9.79E-5
19 SGTC_2479 5617353 3.67 43.9 µM 3.46E-4 50 1.22E-4
20 SGTC_2320 7373003 3.65 200.0 µM 3.66E-4 35 1.31E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.169 6.48E-23 YGL071W AFT1 hom Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.149 5.00E-18 YOR034C-A_p YOR034C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.114 3.22E-11 YLR148W PEP3 hom Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
0.112 8.82E-11 YLR268W SEC22 hom R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
0.111 1.35E-10 YML123C PHO84 hom High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
0.107 5.05E-10 YBR097W VPS15 hom Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
0.107 6.10E-10 YGR160W_d YGR160W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.105 1.12E-9 YDL185W VMA1 hom Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
0.104 1.56E-9 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.103 2.14E-9 YLR056W ERG3 hom C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase
0.102 2.67E-9 YLR396C VPS33 hom ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole
0.102 3.56E-9 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.100 5.51E-9 YER040W GLN3 hom Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source
0.095 3.69E-8 YGR060W ERG25 het C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.094 4.99E-8 YNL051W COG5 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments