YJL128C / PBS2

MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL128C / PBS2

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:5.94|P-value:3.71E-8 ID:SGTC_95|Compound:0471-0001|FD-Score:-8.33|P-value:9.29E-15 ID:SGTC_101|Compound:0490-5701|FD-Score:-5.00|P-value:1.96E-6 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:-4.58|P-value:1.15E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:-4.87|P-value:3.38E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:4.15|P-value:8.89E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:3.53|P-value:7.35E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:6.56|P-value:1.38E-9 ID:SGTC_207|Compound:4185-0010|FD-Score:7.60|P-value:2.59E-12 ID:SGTC_253|Compound:dyclonine|FD-Score:4.70|P-value:1.07E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.86|P-value:5.60E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:5.88|P-value:5.03E-8 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:3.45|P-value:9.43E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:3.62|P-value:5.62E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.15|P-value:9.02E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:4.90|P-value:4.70E-6 ID:SGTC_428|Compound:3448-4153|FD-Score:4.00|P-value:1.53E-4 ID:SGTC_470|Compound:aa-861|FD-Score:5.07|P-value:2.28E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-3.34|P-value:9.60E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.29|P-value:5.42E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:8.54|P-value:4.29E-15 ID:SGTC_759|Compound:3486-0358|FD-Score:3.61|P-value:5.83E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.58|P-value:6.24E-4 ID:SGTC_792|Compound:4534-1957|FD-Score:3.47|P-value:9.04E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:4.48|P-value:2.58E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:3.76|P-value:3.52E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:5.00|P-value:3.09E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-3.44|P-value:7.13E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.53|P-value:7.50E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:7.16|P-value:4.13E-11 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.55|P-value:6.93E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.86|P-value:2.55E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:5.15|P-value:1.57E-6 ID:SGTC_1492|Compound:4158-0558|FD-Score:-3.60|P-value:4.23E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:6.35|P-value:4.36E-9 ID:SGTC_1518|Compound:st038567|FD-Score:3.72|P-value:3.96E-4 ID:SGTC_1663|Compound:st014140|FD-Score:4.43|P-value:3.21E-5 ID:SGTC_1726|Compound:st035556|FD-Score:3.74|P-value:3.78E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.49|P-value:8.32E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.97|P-value:1.69E-4 ID:SGTC_1941|Compound:st076221|FD-Score:4.35|P-value:4.27E-5 ID:SGTC_1974|Compound:st069448|FD-Score:4.52|P-value:2.22E-5 ID:SGTC_2050|Compound:5263056|FD-Score:5.16|P-value:1.49E-6 ID:SGTC_2067|Compound:5224629|FD-Score:6.48|P-value:2.13E-9 ID:SGTC_2072|Compound:5231554|FD-Score:3.53|P-value:7.49E-4 ID:SGTC_2102|Compound:5377816|FD-Score:4.23|P-value:6.83E-5 ID:SGTC_2122|Compound:5258151|FD-Score:3.90|P-value:2.22E-4 ID:SGTC_2368|Compound:9071271|FD-Score:3.52|P-value:7.65E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.56|P-value:6.81E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:6.13|P-value:1.37E-8 ID:SGTC_2415|Compound:st077333|FD-Score:4.12|P-value:1.02E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.91|P-value:4.47E-6 ID:SGTC_2812|Compound:7970281|FD-Score:5.04|P-value:2.55E-6 ID:SGTC_2816|Compound:7982706|FD-Score:3.71|P-value:4.13E-4 ID:SGTC_2889|Compound:9059656|FD-Score:3.76|P-value:3.49E-4 ID:SGTC_2964|Compound:9083345|FD-Score:5.82|P-value:6.62E-8 ID:SGTC_2968|Compound:9090663|FD-Score:3.76|P-value:3.52E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.70|P-value:3.02E-4 ID:SGTC_3278|Compound:9139605|FD-Score:-4.56|P-value:1.22E-5 ID:SGTC_3284|Compound:9108171|FD-Score:-4.00|P-value:1.05E-4 ID:SGTC_3321|Compound:9137793|FD-Score:4.59|P-value:1.66E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:5.94|P-value:3.71E-8 ID:SGTC_95|Compound:0471-0001|FD-Score:-8.33|P-value:9.29E-15 ID:SGTC_101|Compound:0490-5701|FD-Score:-5.00|P-value:1.96E-6 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:-4.58|P-value:1.15E-5 ID:SGTC_108|Compound:0509-0257|FD-Score:-4.87|P-value:3.38E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:4.15|P-value:8.89E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:3.53|P-value:7.35E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:6.56|P-value:1.38E-9 ID:SGTC_207|Compound:4185-0010|FD-Score:7.60|P-value:2.59E-12 ID:SGTC_253|Compound:dyclonine|FD-Score:4.70|P-value:1.07E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.86|P-value:5.60E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:5.88|P-value:5.03E-8 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:3.45|P-value:9.43E-4 ID:SGTC_408|Compound:cantharidin|FD-Score:3.62|P-value:5.62E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.15|P-value:9.02E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:4.90|P-value:4.70E-6 ID:SGTC_428|Compound:3448-4153|FD-Score:4.00|P-value:1.53E-4 ID:SGTC_470|Compound:aa-861|FD-Score:5.07|P-value:2.28E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-3.34|P-value:9.60E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.29|P-value:5.42E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:8.54|P-value:4.29E-15 ID:SGTC_759|Compound:3486-0358|FD-Score:3.61|P-value:5.83E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.58|P-value:6.24E-4 ID:SGTC_792|Compound:4534-1957|FD-Score:3.47|P-value:9.04E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:4.48|P-value:2.58E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:3.76|P-value:3.52E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:5.00|P-value:3.09E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-3.44|P-value:7.13E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.53|P-value:7.50E-4 ID:SGTC_1092|Compound:toremifene|FD-Score:7.16|P-value:4.13E-11 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.55|P-value:6.93E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.86|P-value:2.55E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:5.15|P-value:1.57E-6 ID:SGTC_1492|Compound:4158-0558|FD-Score:-3.60|P-value:4.23E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:6.35|P-value:4.36E-9 ID:SGTC_1518|Compound:st038567|FD-Score:3.72|P-value:3.96E-4 ID:SGTC_1663|Compound:st014140|FD-Score:4.43|P-value:3.21E-5 ID:SGTC_1726|Compound:st035556|FD-Score:3.74|P-value:3.78E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.49|P-value:8.32E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.97|P-value:1.69E-4 ID:SGTC_1941|Compound:st076221|FD-Score:4.35|P-value:4.27E-5 ID:SGTC_1974|Compound:st069448|FD-Score:4.52|P-value:2.22E-5 ID:SGTC_2050|Compound:5263056|FD-Score:5.16|P-value:1.49E-6 ID:SGTC_2067|Compound:5224629|FD-Score:6.48|P-value:2.13E-9 ID:SGTC_2072|Compound:5231554|FD-Score:3.53|P-value:7.49E-4 ID:SGTC_2102|Compound:5377816|FD-Score:4.23|P-value:6.83E-5 ID:SGTC_2122|Compound:5258151|FD-Score:3.90|P-value:2.22E-4 ID:SGTC_2368|Compound:9071271|FD-Score:3.52|P-value:7.65E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.56|P-value:6.81E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:6.13|P-value:1.37E-8 ID:SGTC_2415|Compound:st077333|FD-Score:4.12|P-value:1.02E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.91|P-value:4.47E-6 ID:SGTC_2812|Compound:7970281|FD-Score:5.04|P-value:2.55E-6 ID:SGTC_2816|Compound:7982706|FD-Score:3.71|P-value:4.13E-4 ID:SGTC_2889|Compound:9059656|FD-Score:3.76|P-value:3.49E-4 ID:SGTC_2964|Compound:9083345|FD-Score:5.82|P-value:6.62E-8 ID:SGTC_2968|Compound:9090663|FD-Score:3.76|P-value:3.52E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.70|P-value:3.02E-4 ID:SGTC_3278|Compound:9139605|FD-Score:-4.56|P-value:1.22E-5 ID:SGTC_3284|Compound:9108171|FD-Score:-4.00|P-value:1.05E-4 ID:SGTC_3321|Compound:9137793|FD-Score:4.59|P-value:1.66E-5

Top fitness defect scores for YJL128C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_690 glyoxal 8.54 20.3 µM 4.29E-15 24 6.66E-18
2 SGTC_207 4185-0010 7.60 4.5 µM 2.59E-12 endomembrane recycling 29 1.45E-14
3 SGTC_1092 toremifene 7.16 6.3 µM 4.13E-11 1 4.05E-13
4 SGTC_178 4092-0806 6.56 87.5 µM 1.38E-9 ergosterol depletion effects on membrane 33 2.73E-11
5 SGTC_2067 5224629 6.48 34.1 µM 2.13E-9 3 4.62E-11
6 SGTC_1511 0906-3109 6.35 28.5 µM 4.36E-9 excess fatty acid 11 1.09E-10
7 SGTC_2408 sulbentine 6.13 18.4 µM 1.37E-8 22 4.31E-10
8 SGTC_67 0335-0847 5.94 266.0 µM 3.71E-8 ubiquinone biosynthesis & proteasome 70 1.43E-9
9 SGTC_266 formosanin C 5.88 406.0 nM 5.03E-8 sphingolipid biosynthesis & PDR1 29 2.06E-9
10 SGTC_2964 9083345 5.82 53.4 µM 6.62E-8 5 2.87E-9
11 SGTC_2050 5263056 5.16 171.0 µM 1.49E-6 fatty acid desaturase (OLE1) 23 1.20E-7
12 SGTC_1176 0848-0131 5.15 83.3 µM 1.57E-6 Golgi 7 1.28E-7
13 SGTC_470 aa-861 5.07 153.0 µM 2.28E-6 sphingolipid biosynthesis & PDR1 35 2.01E-7
14 SGTC_2812 7970281 5.04 58.4 µM 2.55E-6 7 2.30E-7
15 SGTC_914 3820-6779 5.00 408.0 µM 3.09E-6 8 2.90E-7
16 SGTC_2651 1327-0036 4.91 5.0 µM 4.47E-6 112 4.51E-7
17 SGTC_415 dibenzyl ketone 4.90 100.0 µM 4.70E-6 6 4.79E-7
18 SGTC_255 tunicamycin 4.86 200.5 nM 5.60E-6 unfolded protein response 46 5.91E-7
19 SGTC_253 dyclonine 4.70 31.2 µM 1.07E-5 amide catabolism 11 1.28E-6
20 SGTC_3321 9137793 4.59 26.8 µM 1.66E-5 cell wall 59 2.18E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.193 1.54E-29 YLR113W HOG1 hom Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress
0.160 1.43E-20 YNR008W LRO1 hom Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase
0.152 6.70E-19 YDR326C YSP2 hom Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
0.136 3.25E-15 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.135 4.27E-15 YNR031C SSK2 hom MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
0.132 1.45E-14 YDR072C IPT1 hom Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
0.128 1.08E-13 YNL307C MCK1 hom Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family
0.123 8.39E-13 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.122 1.12E-12 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.122 1.38E-12 YPL153C RAD53 het Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
0.116 1.33E-11 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.116 2.00E-11 YKL104C GFA1 het Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
0.115 2.14E-11 YNL229C URE2 hom Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
0.114 2.95E-11 YPL057C SUR1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.114 3.15E-11 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder