YJL130C / URA2

Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL130C / URA2

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:4.20|P-value:2.22E-6 ID:SGTC_166|Compound:k072-0058|FD-Score:-6.94|P-value:3.17E-14 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.76|P-value:2.52E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:5.46|P-value:1.33E-9 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.61|P-value:3.92E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:2.92|P-value:7.06E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:-10.70|P-value:3.79E-31 ID:SGTC_425|Compound:3825-7017|FD-Score:-3.39|P-value:1.33E-4 ID:SGTC_433|Compound:pimozide|FD-Score:2.88|P-value:8.08E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:-2.95|P-value:7.50E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.33|P-value:1.72E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:-6.02|P-value:3.87E-11 ID:SGTC_553|Compound:k007-0256|FD-Score:4.00|P-value:6.40E-6 ID:SGTC_556|Compound:4058-0063|FD-Score:-3.75|P-value:2.63E-5 ID:SGTC_623|Compound:k072-0108|FD-Score:4.45|P-value:6.02E-7 ID:SGTC_629|Compound:3448-6466|FD-Score:-3.26|P-value:2.29E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:3.47|P-value:7.48E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:3.80|P-value:1.64E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.29|P-value:1.97E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:3.14|P-value:3.01E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:2.95|P-value:6.12E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:-3.22|P-value:2.60E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.74|P-value:2.77E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.09|P-value:4.01E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:-3.27|P-value:2.14E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:2.99|P-value:5.32E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.59|P-value:5.42E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-7.35|P-value:9.34E-16 ID:SGTC_1111|Compound:tnp00062|FD-Score:-8.24|P-value:2.51E-19 ID:SGTC_1112|Compound:tnp00069|FD-Score:-14.10|P-value:4.31E-53 ID:SGTC_1234|Compound:0326-0494|FD-Score:-3.04|P-value:5.29E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-2.94|P-value:7.90E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:-2.88|P-value:9.67E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.38|P-value:1.08E-4 ID:SGTC_2126|Compound:5251174|FD-Score:7.07|P-value:6.90E-15 ID:SGTC_2296|Compound:7971656|FD-Score:3.27|P-value:1.77E-4 ID:SGTC_2301|Compound:7769476|FD-Score:3.13|P-value:3.04E-4 ID:SGTC_2302|Compound:7771753|FD-Score:-3.81|P-value:2.00E-5 ID:SGTC_2307|Compound:7538926|FD-Score:3.66|P-value:3.14E-5 ID:SGTC_2309|Compound:7664088|FD-Score:3.11|P-value:3.31E-4 ID:SGTC_2318|Compound:6029776|FD-Score:2.84|P-value:9.42E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.10|P-value:3.43E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.17|P-value:3.26E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.57|P-value:4.88E-5 ID:SGTC_2450|Compound:5739737|FD-Score:-3.26|P-value:2.24E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.28|P-value:1.65E-4 ID:SGTC_2797|Compound:7859098|FD-Score:4.03|P-value:5.44E-6 ID:SGTC_2809|Compound:7964133|FD-Score:9.83|P-value:5.78E-27 ID:SGTC_2812|Compound:7970281|FD-Score:-3.26|P-value:2.26E-4 ID:SGTC_2814|Compound:7977482|FD-Score:4.85|P-value:5.94E-8 ID:SGTC_2831|Compound:7999036|FD-Score:3.95|P-value:7.90E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-9.13|P-value:2.59E-23 ID:SGTC_2874|Compound:9043904|FD-Score:3.30|P-value:1.56E-4 ID:SGTC_2938|Compound:9059746|FD-Score:3.20|P-value:2.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:3.87|P-value:1.20E-5 ID:SGTC_2968|Compound:9090663|FD-Score:-2.99|P-value:6.56E-4 ID:SGTC_2971|Compound:9092186|FD-Score:4.75|P-value:1.10E-7 ID:SGTC_2973|Compound:9094665|FD-Score:3.21|P-value:2.25E-4 ID:SGTC_3001|Compound:9076041|FD-Score:3.85|P-value:1.31E-5 ID:SGTC_3004|Compound:9076122|FD-Score:-3.00|P-value:6.19E-4 ID:SGTC_3010|Compound:9080089|FD-Score:4.44|P-value:6.15E-7 ID:SGTC_3018|Compound:9082602|FD-Score:5.90|P-value:6.37E-11 ID:SGTC_3091|Compound:9116372|FD-Score:-3.06|P-value:5.02E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-4.40|P-value:1.02E-6 ID:SGTC_16|Compound:erodoxin|FD-Score:4.20|P-value:2.22E-6 ID:SGTC_166|Compound:k072-0058|FD-Score:-6.94|P-value:3.17E-14 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.76|P-value:2.52E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:5.46|P-value:1.33E-9 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.61|P-value:3.92E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:2.92|P-value:7.06E-4 ID:SGTC_368|Compound:fentichlor|FD-Score:-10.70|P-value:3.79E-31 ID:SGTC_425|Compound:3825-7017|FD-Score:-3.39|P-value:1.33E-4 ID:SGTC_433|Compound:pimozide|FD-Score:2.88|P-value:8.08E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:-2.95|P-value:7.50E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.33|P-value:1.72E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:-6.02|P-value:3.87E-11 ID:SGTC_553|Compound:k007-0256|FD-Score:4.00|P-value:6.40E-6 ID:SGTC_556|Compound:4058-0063|FD-Score:-3.75|P-value:2.63E-5 ID:SGTC_623|Compound:k072-0108|FD-Score:4.45|P-value:6.02E-7 ID:SGTC_629|Compound:3448-6466|FD-Score:-3.26|P-value:2.29E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:3.47|P-value:7.48E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:3.80|P-value:1.64E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.29|P-value:1.97E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:3.14|P-value:3.01E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:2.95|P-value:6.12E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:-3.22|P-value:2.60E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.74|P-value:2.77E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.09|P-value:4.01E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:-3.27|P-value:2.14E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:2.99|P-value:5.32E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.59|P-value:5.42E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-7.35|P-value:9.34E-16 ID:SGTC_1111|Compound:tnp00062|FD-Score:-8.24|P-value:2.51E-19 ID:SGTC_1112|Compound:tnp00069|FD-Score:-14.10|P-value:4.31E-53 ID:SGTC_1234|Compound:0326-0494|FD-Score:-3.04|P-value:5.29E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-2.94|P-value:7.90E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:-2.88|P-value:9.67E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.38|P-value:1.08E-4 ID:SGTC_2126|Compound:5251174|FD-Score:7.07|P-value:6.90E-15 ID:SGTC_2296|Compound:7971656|FD-Score:3.27|P-value:1.77E-4 ID:SGTC_2301|Compound:7769476|FD-Score:3.13|P-value:3.04E-4 ID:SGTC_2302|Compound:7771753|FD-Score:-3.81|P-value:2.00E-5 ID:SGTC_2307|Compound:7538926|FD-Score:3.66|P-value:3.14E-5 ID:SGTC_2309|Compound:7664088|FD-Score:3.11|P-value:3.31E-4 ID:SGTC_2318|Compound:6029776|FD-Score:2.84|P-value:9.42E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.10|P-value:3.43E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.17|P-value:3.26E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.57|P-value:4.88E-5 ID:SGTC_2450|Compound:5739737|FD-Score:-3.26|P-value:2.24E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.28|P-value:1.65E-4 ID:SGTC_2797|Compound:7859098|FD-Score:4.03|P-value:5.44E-6 ID:SGTC_2809|Compound:7964133|FD-Score:9.83|P-value:5.78E-27 ID:SGTC_2812|Compound:7970281|FD-Score:-3.26|P-value:2.26E-4 ID:SGTC_2814|Compound:7977482|FD-Score:4.85|P-value:5.94E-8 ID:SGTC_2831|Compound:7999036|FD-Score:3.95|P-value:7.90E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-9.13|P-value:2.59E-23 ID:SGTC_2874|Compound:9043904|FD-Score:3.30|P-value:1.56E-4 ID:SGTC_2938|Compound:9059746|FD-Score:3.20|P-value:2.34E-4 ID:SGTC_2965|Compound:9082772|FD-Score:3.87|P-value:1.20E-5 ID:SGTC_2968|Compound:9090663|FD-Score:-2.99|P-value:6.56E-4 ID:SGTC_2971|Compound:9092186|FD-Score:4.75|P-value:1.10E-7 ID:SGTC_2973|Compound:9094665|FD-Score:3.21|P-value:2.25E-4 ID:SGTC_3001|Compound:9076041|FD-Score:3.85|P-value:1.31E-5 ID:SGTC_3004|Compound:9076122|FD-Score:-3.00|P-value:6.19E-4 ID:SGTC_3010|Compound:9080089|FD-Score:4.44|P-value:6.15E-7 ID:SGTC_3018|Compound:9082602|FD-Score:5.90|P-value:6.37E-11 ID:SGTC_3091|Compound:9116372|FD-Score:-3.06|P-value:5.02E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-4.40|P-value:1.02E-6

Top fitness defect scores for YJL130C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2809 7964133 9.83 13.0 µM 5.78E-27 60S ribosome export 1 4.37E-23
2 SGTC_2126 5251174 7.07 33.9 µM 6.90E-15 1 7.78E-13
3 SGTC_3018 9082602 5.90 49.5 µM 6.37E-11 30 1.81E-9
4 SGTC_171 k064-0041 5.46 9.7 µM 1.33E-9 heme requiring 11 2.39E-8
5 SGTC_2814 7977482 4.85 43.0 µM 5.94E-8 25 6.03E-7
6 SGTC_2971 9092186 4.75 9.9 µM 1.10E-7 ergosterol biosynthesis 15 1.02E-6
7 SGTC_623 k072-0108 4.45 16.9 µM 6.02E-7 heme biosynthesis & mitochondrial translocase 6 4.32E-6
8 SGTC_3010 9080089 4.44 71.4 µM 6.15E-7 7 4.39E-6
9 SGTC_16 erodoxin 4.20 22.8 µM 2.22E-6 unfolded protein response 58 1.31E-5
10 SGTC_1044 1222-0021 4.09 1.8 µM 4.01E-6 heme biosynthesis & mitochondrial translocase 32 2.17E-5
11 SGTC_2797 7859098 4.03 81.8 µM 5.44E-6 27 2.81E-5
12 SGTC_553 k007-0256 4.00 45.7 µM 6.40E-6 heme biosynthesis & mitochondrial translocase 19 3.22E-5
13 SGTC_2831 7999036 3.95 19.5 µM 7.90E-6 47 3.86E-5
14 SGTC_2965 9082772 3.87 38.6 µM 1.20E-5 mitochondrial response to ROS 43 5.52E-5
15 SGTC_3001 9076041 3.85 71.4 µM 1.31E-5 16 5.91E-5
16 SGTC_690 glyoxal 3.80 20.3 µM 1.64E-5 88 7.19E-5
17 SGTC_2307 7538926 3.66 6.3 µM 3.14E-5 50 1.25E-4
18 SGTC_255 tunicamycin 3.61 200.5 nM 3.92E-5 unfolded protein response 93 1.51E-4
19 SGTC_2341 7987486 3.57 176.7 µM 4.88E-5 29 1.82E-4
20 SGTC_670 4204-0025 3.47 119.0 µM 7.48E-5 copper-dependent oxidative stress 46 2.61E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.193 1.26E-29 YML021C UNG1 hom Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
0.176 9.34E-25 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.168 1.20E-22 YGL158W RCK1 hom Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication
0.168 1.29E-22 YOR286W RDL2 hom Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss
0.161 5.82E-21 YJL032W_d YJL032W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
0.155 1.70E-19 YNL305C BXI1 hom Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
0.132 2.24E-14 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.128 9.97E-14 YLL051C FRE6 hom Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels
0.128 1.03E-13 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.123 8.66E-13 YDR056C_p YDR056C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein
0.122 1.47E-12 YJR031C GEA1 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
0.117 1.32E-11 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.113 6.67E-11 YKR045C_p YKR045C_p hom Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
0.108 3.36E-10 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.108 3.63E-10 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress