YJL131C / AIM23

Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL131C / AIM23

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:4.89|P-value:1.04E-7 ID:SGTC_158|Compound:k038-0087|FD-Score:3.56|P-value:7.87E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:3.30|P-value:2.26E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.25|P-value:2.76E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:3.17|P-value:3.91E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.11|P-value:4.85E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:2.97|P-value:8.14E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.27|P-value:2.55E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.25|P-value:2.76E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:-2.96|P-value:8.49E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.26|P-value:2.69E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-4.82|P-value:1.61E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.02|P-value:9.86E-6 ID:SGTC_1327|Compound:1436-0082|FD-Score:-3.32|P-value:2.14E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.63|P-value:5.85E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.16|P-value:5.13E-6 ID:SGTC_1744|Compound:st037937|FD-Score:3.87|P-value:2.04E-5 ID:SGTC_1746|Compound:st038117|FD-Score:-3.58|P-value:7.33E-5 ID:SGTC_1833|Compound:st055984|FD-Score:3.61|P-value:6.25E-5 ID:SGTC_1842|Compound:st055362|FD-Score:2.93|P-value:9.26E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.95|P-value:1.39E-5 ID:SGTC_2035|Compound:5154463|FD-Score:3.02|P-value:6.81E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.60|P-value:6.71E-5 ID:SGTC_2081|Compound:5214970|FD-Score:3.09|P-value:5.23E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.00|P-value:7.17E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-6.08|P-value:5.34E-11 ID:SGTC_2400|Compound:5203494|FD-Score:-4.78|P-value:1.98E-7 ID:SGTC_2435|Compound:5706476|FD-Score:3.11|P-value:4.83E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.05|P-value:5.96E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-2.92|P-value:9.87E-4 ID:SGTC_2880|Compound:9042983|FD-Score:4.56|P-value:6.54E-7 ID:SGTC_2913|Compound:7966556|FD-Score:3.26|P-value:2.73E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.44|P-value:1.30E-4 ID:SGTC_2947|Compound:9072639|FD-Score:4.95|P-value:7.35E-8 ID:SGTC_3002|Compound:9075603|FD-Score:-3.19|P-value:3.57E-4 ID:SGTC_3109|Compound:9122853|FD-Score:3.65|P-value:5.39E-5 ID:SGTC_3124|Compound:9127567|FD-Score:-2.97|P-value:8.12E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:4.89|P-value:1.04E-7 ID:SGTC_158|Compound:k038-0087|FD-Score:3.56|P-value:7.87E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:3.30|P-value:2.26E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.25|P-value:2.76E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:3.17|P-value:3.91E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.11|P-value:4.85E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:2.97|P-value:8.14E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.27|P-value:2.55E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.25|P-value:2.76E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:-2.96|P-value:8.49E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.26|P-value:2.69E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-4.82|P-value:1.61E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.02|P-value:9.86E-6 ID:SGTC_1327|Compound:1436-0082|FD-Score:-3.32|P-value:2.14E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.63|P-value:5.85E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.16|P-value:5.13E-6 ID:SGTC_1744|Compound:st037937|FD-Score:3.87|P-value:2.04E-5 ID:SGTC_1746|Compound:st038117|FD-Score:-3.58|P-value:7.33E-5 ID:SGTC_1833|Compound:st055984|FD-Score:3.61|P-value:6.25E-5 ID:SGTC_1842|Compound:st055362|FD-Score:2.93|P-value:9.26E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.95|P-value:1.39E-5 ID:SGTC_2035|Compound:5154463|FD-Score:3.02|P-value:6.81E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.60|P-value:6.71E-5 ID:SGTC_2081|Compound:5214970|FD-Score:3.09|P-value:5.23E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.00|P-value:7.17E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-6.08|P-value:5.34E-11 ID:SGTC_2400|Compound:5203494|FD-Score:-4.78|P-value:1.98E-7 ID:SGTC_2435|Compound:5706476|FD-Score:3.11|P-value:4.83E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.05|P-value:5.96E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-2.92|P-value:9.87E-4 ID:SGTC_2880|Compound:9042983|FD-Score:4.56|P-value:6.54E-7 ID:SGTC_2913|Compound:7966556|FD-Score:3.26|P-value:2.73E-4 ID:SGTC_2933|Compound:9026794|FD-Score:3.44|P-value:1.30E-4 ID:SGTC_2947|Compound:9072639|FD-Score:4.95|P-value:7.35E-8 ID:SGTC_3002|Compound:9075603|FD-Score:-3.19|P-value:3.57E-4 ID:SGTC_3109|Compound:9122853|FD-Score:3.65|P-value:5.39E-5 ID:SGTC_3124|Compound:9127567|FD-Score:-2.97|P-value:8.12E-4

Top fitness defect scores for YJL131C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2947 9072639 4.95 7.7 µM 7.35E-8 tubulin folding & SWR complex 17 3.66E-7
2 SGTC_67 0335-0847 4.89 266.0 µM 1.04E-7 ubiquinone biosynthesis & proteasome 87 4.99E-7
3 SGTC_2880 9042983 4.56 58.4 µM 6.54E-7 fatty acid desaturase (OLE1) 13 2.57E-6
4 SGTC_1043 0916-0009 4.02 19.3 µM 9.86E-6 20 2.89E-5
5 SGTC_1744 st037937 3.87 38.9 µM 2.04E-5 31 5.53E-5
6 SGTC_3109 9122853 3.65 49.5 µM 5.39E-5 10 1.32E-4
7 SGTC_1833 st055984 3.61 71.0 µM 6.25E-5 23 1.51E-4
8 SGTC_2079 5212404 3.60 121.0 µM 6.71E-5 19 1.60E-4
9 SGTC_158 k038-0087 3.56 26.4 µM 7.87E-5 ubiquinone biosynthesis & proteasome 169 1.85E-4
10 SGTC_2933 9026794 3.44 32.5 µM 1.30E-4 46 2.89E-4
11 SGTC_182 3448-4371 3.30 550.5 µM 2.26E-4 45 4.76E-4
12 SGTC_514 4466-0038 3.27 2.6 nM 2.55E-4 cell wall 207 5.30E-4
13 SGTC_2913 7966556 3.26 36.6 µM 2.73E-4 60S ribosome export 36 5.63E-4
14 SGTC_699 0375-0592 3.25 111.0 µM 2.76E-4 iron homeostasis 49 5.67E-4
15 SGTC_294 k038-0071 3.17 157.0 µM 3.91E-4 ubiquinone biosynthesis & proteasome 102 7.75E-4
16 SGTC_2435 5706476 3.11 10.6 µM 4.83E-4 59 9.36E-4
17 SGTC_2081 5214970 3.09 32.3 µM 5.23E-4 61 0.00101
18 SGTC_2035 5154463 3.02 94.6 µM 6.81E-4 55 0.00127
19 SGTC_2350 9023895 3.00 38.9 µM 7.17E-4 ergosterol biosynthesis 72 0.00133
20 SGTC_410 α-linolenic acid 2.97 100.0 µM 8.14E-4 excess fatty acid 70 0.00150

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.223 5.10E-39 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.194 8.35E-30 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene
0.176 8.95E-25 YDL131W LYS21 hom Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
0.170 4.33E-23 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.169 4.91E-23 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.165 7.40E-22 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.162 3.90E-21 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.156 8.06E-20 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.150 2.71E-18 YPL192C PRM3 hom Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body
0.149 4.92E-18 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.145 3.81E-17 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.141 1.88E-16 YDL197C ASF2 hom Anti-silencing protein that causes derepression of silent loci when overexpressed
0.140 4.84E-16 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.139 8.82E-16 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.138 1.08E-15 YLR378C SEC61 het Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER