YJL141C / YAK1

Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL141C / YAK1

Click on Significant Values for Screen Details ID:SGTC_95|Compound:0471-0001|FD-Score:3.10|P-value:4.51E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.39|P-value:1.39E-4 ID:SGTC_220|Compound:paclitaxel|FD-Score:3.03|P-value:5.73E-4 ID:SGTC_230|Compound:MPP+|FD-Score:3.39|P-value:1.36E-4 ID:SGTC_417|Compound:st075190|FD-Score:-2.86|P-value:9.96E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.15|P-value:3.63E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.15|P-value:3.64E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:4.44|P-value:9.62E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:7.11|P-value:1.11E-14 ID:SGTC_577|Compound:r071-0003|FD-Score:4.93|P-value:6.05E-8 ID:SGTC_618|Compound:k072-0232|FD-Score:4.32|P-value:1.76E-6 ID:SGTC_654|Compound:1124-0271|FD-Score:3.28|P-value:2.21E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:4.87|P-value:8.72E-8 ID:SGTC_775|Compound:4577-0580|FD-Score:3.06|P-value:5.11E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:4.73|P-value:1.90E-7 ID:SGTC_880|Compound:0833-0487|FD-Score:3.42|P-value:1.25E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-2.99|P-value:6.23E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:3.42|P-value:1.22E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:3.51|P-value:8.24E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:4.04|P-value:7.35E-6 ID:SGTC_974|Compound:1326-0144|FD-Score:3.51|P-value:8.50E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.28|P-value:2.22E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:4.12|P-value:4.89E-6 ID:SGTC_1408|Compound:3909-7976|FD-Score:-3.18|P-value:3.02E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:-3.08|P-value:4.43E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.34|P-value:1.69E-4 ID:SGTC_1616|Compound:st002734|FD-Score:3.54|P-value:7.45E-5 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:2.93|P-value:8.41E-4 ID:SGTC_1885|Compound:st057546|FD-Score:4.46|P-value:8.70E-7 ID:SGTC_1982|Compound:st072467|FD-Score:4.42|P-value:1.04E-6 ID:SGTC_2304|Compound:7443016|FD-Score:-3.91|P-value:1.22E-5 ID:SGTC_2380|Compound:9035439|FD-Score:4.72|P-value:2.05E-7 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.82|P-value:1.18E-7 ID:SGTC_2646|Compound:retinol|FD-Score:2.96|P-value:7.69E-4 ID:SGTC_2652|Compound:actinonin|FD-Score:-3.09|P-value:4.32E-4 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:-3.60|P-value:5.16E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.04|P-value:5.64E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:-3.51|P-value:7.65E-5 ID:SGTC_2871|Compound:9038510|FD-Score:2.98|P-value:7.03E-4 ID:SGTC_2908|Compound:7930301|FD-Score:3.26|P-value:2.36E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.78|P-value:2.47E-5 ID:SGTC_2972|Compound:9093619|FD-Score:-4.65|P-value:2.65E-7 ID:SGTC_2974|Compound:9094440|FD-Score:2.90|P-value:9.26E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.79|P-value:2.40E-5 ID:SGTC_3055|Compound:9094636|FD-Score:3.18|P-value:3.21E-4 ID:SGTC_3082|Compound:9117008|FD-Score:3.47|P-value:9.93E-5 ID:SGTC_3148|Compound:9097893|FD-Score:3.01|P-value:6.17E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:3.10|P-value:4.51E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.39|P-value:1.39E-4 ID:SGTC_220|Compound:paclitaxel|FD-Score:3.03|P-value:5.73E-4 ID:SGTC_230|Compound:MPP+|FD-Score:3.39|P-value:1.36E-4 ID:SGTC_417|Compound:st075190|FD-Score:-2.86|P-value:9.96E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.15|P-value:3.63E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.15|P-value:3.64E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:4.44|P-value:9.62E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:7.11|P-value:1.11E-14 ID:SGTC_577|Compound:r071-0003|FD-Score:4.93|P-value:6.05E-8 ID:SGTC_618|Compound:k072-0232|FD-Score:4.32|P-value:1.76E-6 ID:SGTC_654|Compound:1124-0271|FD-Score:3.28|P-value:2.21E-4 ID:SGTC_665|Compound:3914-0008|FD-Score:4.87|P-value:8.72E-8 ID:SGTC_775|Compound:4577-0580|FD-Score:3.06|P-value:5.11E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:4.73|P-value:1.90E-7 ID:SGTC_880|Compound:0833-0487|FD-Score:3.42|P-value:1.25E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-2.99|P-value:6.23E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:3.42|P-value:1.22E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:3.51|P-value:8.24E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:4.04|P-value:7.35E-6 ID:SGTC_974|Compound:1326-0144|FD-Score:3.51|P-value:8.50E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.28|P-value:2.22E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:4.12|P-value:4.89E-6 ID:SGTC_1408|Compound:3909-7976|FD-Score:-3.18|P-value:3.02E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:-3.08|P-value:4.43E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.34|P-value:1.69E-4 ID:SGTC_1616|Compound:st002734|FD-Score:3.54|P-value:7.45E-5 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:2.93|P-value:8.41E-4 ID:SGTC_1885|Compound:st057546|FD-Score:4.46|P-value:8.70E-7 ID:SGTC_1982|Compound:st072467|FD-Score:4.42|P-value:1.04E-6 ID:SGTC_2304|Compound:7443016|FD-Score:-3.91|P-value:1.22E-5 ID:SGTC_2380|Compound:9035439|FD-Score:4.72|P-value:2.05E-7 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.82|P-value:1.18E-7 ID:SGTC_2646|Compound:retinol|FD-Score:2.96|P-value:7.69E-4 ID:SGTC_2652|Compound:actinonin|FD-Score:-3.09|P-value:4.32E-4 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:-3.60|P-value:5.16E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.04|P-value:5.64E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:-3.51|P-value:7.65E-5 ID:SGTC_2871|Compound:9038510|FD-Score:2.98|P-value:7.03E-4 ID:SGTC_2908|Compound:7930301|FD-Score:3.26|P-value:2.36E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.78|P-value:2.47E-5 ID:SGTC_2972|Compound:9093619|FD-Score:-4.65|P-value:2.65E-7 ID:SGTC_2974|Compound:9094440|FD-Score:2.90|P-value:9.26E-4 ID:SGTC_3054|Compound:9094397|FD-Score:3.79|P-value:2.40E-5 ID:SGTC_3055|Compound:9094636|FD-Score:3.18|P-value:3.21E-4 ID:SGTC_3082|Compound:9117008|FD-Score:3.47|P-value:9.93E-5 ID:SGTC_3148|Compound:9097893|FD-Score:3.01|P-value:6.17E-4

Top fitness defect scores for YJL141C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_568 1193-0097 7.11 7.5 µM 1.11E-14 ERAD & cell cycle 46 5.76E-13
2 SGTC_577 r071-0003 4.93 38.6 µM 6.05E-8 fatty acid desaturase (OLE1) 27 4.09E-7
3 SGTC_665 3914-0008 4.87 307.0 µM 8.72E-8 7 5.63E-7
4 SGTC_2636 apigenin dimethyl ether 4.82 100.0 µM 1.18E-7 19 7.35E-7
5 SGTC_870 0573-2213 4.73 57.6 µM 1.90E-7 ERG2 8 1.11E-6
6 SGTC_2380 9035439 4.72 200.0 µM 2.05E-7 endomembrane recycling 7 1.19E-6
7 SGTC_1885 st057546 4.46 99.9 µM 8.70E-7 11 4.17E-6
8 SGTC_538 2922-0838 4.44 53.3 µM 9.62E-7 Golgi 22 4.56E-6
9 SGTC_1982 st072467 4.42 71.9 µM 1.04E-6 27 4.86E-6
10 SGTC_618 k072-0232 4.32 33.2 µM 1.76E-6 60S ribosome export 6 7.72E-6
11 SGTC_1323 1348-1351 4.12 11.2 µM 4.89E-6 5 1.88E-5
12 SGTC_971 1315-0384 4.04 26.7 µM 7.35E-6 cell wall signaling 19 2.68E-5
13 SGTC_3054 9094397 3.79 49.5 µM 2.40E-5 fatty acid desaturase (OLE1) 36 7.53E-5
14 SGTC_2953 9074162 3.78 66.5 µM 2.47E-5 30 7.72E-5
15 SGTC_1616 st002734 3.54 56.1 µM 7.45E-5 45 2.03E-4
16 SGTC_914 3820-6779 3.51 408.0 µM 8.24E-5 36 2.21E-4
17 SGTC_974 1326-0144 3.51 138.0 µM 8.50E-5 ERG2 63 2.27E-4
18 SGTC_3082 9117008 3.47 49.5 µM 9.93E-5 31 2.60E-4
19 SGTC_886 0866-0150 3.42 153.0 µM 1.22E-4 37 3.11E-4
20 SGTC_880 0833-0487 3.42 87.2 µM 1.25E-4 mitochondrial processes 25 3.19E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.113 4.79E-11 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.107 4.87E-10 YDL001W RMD1 hom Cytoplasmic protein required for sporulation
0.107 4.94E-10 YKL039W PTM1 hom Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
0.106 6.24E-10 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.105 8.93E-10 YHR096C HXT5 hom Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication
0.105 9.47E-10 YGR060W ERG25 het C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.098 1.26E-8 YBR066C NRG2 hom Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication
0.096 2.63E-8 YMR116C ASC1 hom G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation
0.093 5.97E-8 YML120C NDI1 hom NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID
0.091 1.46E-7 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.090 1.90E-7 YER136W GDI1 het GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.087 4.51E-7 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.084 1.03E-6 YLL007C_p YLL007C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene
0.084 1.20E-6 YHR101C BIG1 het Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
0.083 1.62E-6 YML072C TCB3 hom Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact