YJL159W / HSP150

O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL159W / HSP150

Click on Significant Values for Screen Details ID:SGTC_5|Compound:0352-0601|FD-Score:2.98|P-value:7.70E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:4.73|P-value:2.64E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:3.94|P-value:1.41E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:7.49|P-value:1.06E-15 ID:SGTC_206|Compound:4546-0025|FD-Score:7.71|P-value:1.59E-16 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.42|P-value:5.11E-12 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.18|P-value:3.61E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.98|P-value:1.19E-5 ID:SGTC_315|Compound:k915-0449|FD-Score:2.95|P-value:8.47E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:-3.16|P-value:4.52E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.32|P-value:2.28E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:7.92|P-value:2.59E-17 ID:SGTC_542|Compound:3381-0244|FD-Score:4.25|P-value:3.20E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:3.76|P-value:3.31E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:4.15|P-value:5.33E-6 ID:SGTC_649|Compound:1123-0311|FD-Score:3.78|P-value:2.97E-5 ID:SGTC_710|Compound:3448-4187|FD-Score:5.84|P-value:3.10E-10 ID:SGTC_752|Compound:1896-0090|FD-Score:-3.01|P-value:8.02E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:-3.38|P-value:1.94E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:4.30|P-value:2.51E-6 ID:SGTC_903|Compound:1134-0042|FD-Score:-3.65|P-value:6.30E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:9.83|P-value:1.25E-25 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.66|P-value:5.98E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:5.25|P-value:1.31E-8 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.21|P-value:3.25E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.58|P-value:7.31E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:6.45|P-value:4.20E-12 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.93|P-value:1.85E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.74|P-value:4.23E-5 ID:SGTC_1652|Compound:st011932|FD-Score:2.99|P-value:7.33E-4 ID:SGTC_1669|Compound:st015249|FD-Score:-3.16|P-value:4.64E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.29|P-value:2.36E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.15|P-value:4.08E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.92|P-value:1.57E-5 ID:SGTC_1948|Compound:st076329|FD-Score:2.95|P-value:8.41E-4 ID:SGTC_2257|Compound:7960413|FD-Score:3.42|P-value:1.38E-4 ID:SGTC_2350|Compound:9023895|FD-Score:4.16|P-value:5.12E-6 ID:SGTC_2388|Compound:9080168|FD-Score:-3.32|P-value:2.46E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.75|P-value:3.47E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.95|P-value:1.37E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:6.22|P-value:2.23E-11 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.16|P-value:5.10E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:35.50|P-value:0 ID:SGTC_2624|Compound:nerol|FD-Score:-4.26|P-value:3.89E-6 ID:SGTC_2625|Compound:euphol|FD-Score:4.95|P-value:7.79E-8 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.14|P-value:4.29E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.05|P-value:5.82E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.70|P-value:4.21E-5 ID:SGTC_2792|Compound:7535235|FD-Score:-3.92|P-value:1.88E-5 ID:SGTC_3294|Compound:9122953|FD-Score:-3.31|P-value:2.54E-4 ID:SGTC_3298|Compound:9116858|FD-Score:-3.01|P-value:7.90E-4 ID:SGTC_3311|Compound:9131146|FD-Score:5.86|P-value:2.73E-10 ID:SGTC_3321|Compound:9137793|FD-Score:5.16|P-value:2.22E-8 ID:SGTC_3322|Compound:9139058|FD-Score:3.80|P-value:2.79E-5 ID:SGTC_3350|Compound:9150499|FD-Score:7.73|P-value:1.37E-16 ID:SGTC_3351|Compound:9150312|FD-Score:3.52|P-value:9.41E-5 ID:SGTC_5|Compound:0352-0601|FD-Score:2.98|P-value:7.70E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:4.73|P-value:2.64E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:3.94|P-value:1.41E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:7.49|P-value:1.06E-15 ID:SGTC_206|Compound:4546-0025|FD-Score:7.71|P-value:1.59E-16 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.42|P-value:5.11E-12 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.18|P-value:3.61E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.98|P-value:1.19E-5 ID:SGTC_315|Compound:k915-0449|FD-Score:2.95|P-value:8.47E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:-3.16|P-value:4.52E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.32|P-value:2.28E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:7.92|P-value:2.59E-17 ID:SGTC_542|Compound:3381-0244|FD-Score:4.25|P-value:3.20E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:3.76|P-value:3.31E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:4.15|P-value:5.33E-6 ID:SGTC_649|Compound:1123-0311|FD-Score:3.78|P-value:2.97E-5 ID:SGTC_710|Compound:3448-4187|FD-Score:5.84|P-value:3.10E-10 ID:SGTC_752|Compound:1896-0090|FD-Score:-3.01|P-value:8.02E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:-3.38|P-value:1.94E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:4.30|P-value:2.51E-6 ID:SGTC_903|Compound:1134-0042|FD-Score:-3.65|P-value:6.30E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:9.83|P-value:1.25E-25 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.66|P-value:5.98E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:5.25|P-value:1.31E-8 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.21|P-value:3.25E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.58|P-value:7.31E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:6.45|P-value:4.20E-12 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.93|P-value:1.85E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.74|P-value:4.23E-5 ID:SGTC_1652|Compound:st011932|FD-Score:2.99|P-value:7.33E-4 ID:SGTC_1669|Compound:st015249|FD-Score:-3.16|P-value:4.64E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.29|P-value:2.36E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.15|P-value:4.08E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.92|P-value:1.57E-5 ID:SGTC_1948|Compound:st076329|FD-Score:2.95|P-value:8.41E-4 ID:SGTC_2257|Compound:7960413|FD-Score:3.42|P-value:1.38E-4 ID:SGTC_2350|Compound:9023895|FD-Score:4.16|P-value:5.12E-6 ID:SGTC_2388|Compound:9080168|FD-Score:-3.32|P-value:2.46E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.75|P-value:3.47E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.95|P-value:1.37E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:6.22|P-value:2.23E-11 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.16|P-value:5.10E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:35.50|P-value:0 ID:SGTC_2624|Compound:nerol|FD-Score:-4.26|P-value:3.89E-6 ID:SGTC_2625|Compound:euphol|FD-Score:4.95|P-value:7.79E-8 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.14|P-value:4.29E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.05|P-value:5.82E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.70|P-value:4.21E-5 ID:SGTC_2792|Compound:7535235|FD-Score:-3.92|P-value:1.88E-5 ID:SGTC_3294|Compound:9122953|FD-Score:-3.31|P-value:2.54E-4 ID:SGTC_3298|Compound:9116858|FD-Score:-3.01|P-value:7.90E-4 ID:SGTC_3311|Compound:9131146|FD-Score:5.86|P-value:2.73E-10 ID:SGTC_3321|Compound:9137793|FD-Score:5.16|P-value:2.22E-8 ID:SGTC_3322|Compound:9139058|FD-Score:3.80|P-value:2.79E-5 ID:SGTC_3350|Compound:9150499|FD-Score:7.73|P-value:1.37E-16 ID:SGTC_3351|Compound:9150312|FD-Score:3.52|P-value:9.41E-5

Top fitness defect scores for YJL159W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2619 obliquin 35.50 87.9 µM 0 cell wall 1 1.48E-276
2 SGTC_916 4466-0038 9.83 42.0 nM 1.25E-25 cell wall 7 4.27E-23
3 SGTC_514 4466-0038 7.92 2.6 nM 2.59E-17 cell wall 42 1.21E-15
4 SGTC_3350 9150499 7.73 3.6 µM 1.37E-16 cell wall 8 5.38E-15
5 SGTC_206 4546-0025 7.71 72.0 µM 1.59E-16 NEO1 24 6.16E-15
6 SGTC_138 0850-0259 7.49 40.9 µM 1.06E-15 cell wall 69 3.35E-14
7 SGTC_1282 0886-0010 6.45 32.4 µM 4.20E-12 endomembrane recycling 8 5.63E-11
8 SGTC_221 trifluoperazine 6.42 10.3 µM 5.11E-12 NEO1-PIK1 112 6.72E-11
9 SGTC_2584 androsterone 6.22 48.7 µM 2.23E-11 cell wall 53 2.52E-10
10 SGTC_3311 9131146 5.86 2.9 µM 2.73E-10 cell wall 61 2.38E-9
11 SGTC_710 3448-4187 5.84 58.0 µM 3.10E-10 fatty acid desaturase (OLE1) 20 2.66E-9
12 SGTC_1230 0170-0365 5.25 3.8 µM 1.31E-8 endomembrane recycling 33 7.64E-8
13 SGTC_3321 9137793 5.16 26.8 µM 2.22E-8 cell wall 41 1.22E-7
14 SGTC_2625 euphol 4.95 100.0 µM 7.79E-8 14 3.77E-7
15 SGTC_15 0266-0086 4.73 27.1 µM 2.64E-7 endomembrane recycling 36 1.13E-6
16 SGTC_453 enantio-paf c16 4.32 10.0 µM 2.28E-6 118 7.80E-6
17 SGTC_813 k007-0158 4.30 173.0 µM 2.51E-6 fatty acid desaturase (OLE1) 35 8.49E-6
18 SGTC_542 3381-0244 4.25 89.3 µM 3.20E-6 33 1.06E-5
19 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 4.16 70.0 µM 5.10E-6 redox potentiating 68 1.60E-5
20 SGTC_2350 9023895 4.16 38.9 µM 5.12E-6 ergosterol biosynthesis 24 1.61E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.486 1.92E-198 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.477 4.89E-190 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.456 1.90E-171 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.418 1.23E-141 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.385 5.47E-119 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.377 1.75E-113 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.371 3.02E-110 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.362 1.58E-104 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.351 5.26E-98 YEL061C CIN8 hom Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
0.325 1.97E-83 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.319 2.33E-80 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.315 2.09E-78 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.315 3.90E-78 YLR212C TUB4 het Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
0.315 5.16E-78 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.313 2.36E-77 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p