YJL165C / HAL5

Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL165C / HAL5

Click on Significant Values for Screen Details ID:SGTC_33|Compound:0986-0033|FD-Score:4.07|P-value:7.94E-6 ID:SGTC_40|Compound:0327-0103|FD-Score:5.37|P-value:6.71E-9 ID:SGTC_54|Compound:0159-0055|FD-Score:4.83|P-value:1.61E-7 ID:SGTC_57|Compound:miconazole|FD-Score:3.01|P-value:6.98E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:6.95|P-value:1.09E-13 ID:SGTC_187|Compound:1488-2104|FD-Score:23.60|P-value:2.32E-136 ID:SGTC_211|Compound:3455-0679|FD-Score:3.90|P-value:1.79E-5 ID:SGTC_298|Compound:1391-0729|FD-Score:5.45|P-value:4.03E-9 ID:SGTC_304|Compound:1310-0298|FD-Score:-2.99|P-value:9.42E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.13|P-value:5.52E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.16|P-value:5.20E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:5.32|P-value:9.19E-9 ID:SGTC_383|Compound:2324-0395|FD-Score:3.57|P-value:7.54E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:16.80|P-value:3.84E-70 ID:SGTC_502|Compound:l-744,832|FD-Score:5.57|P-value:1.92E-9 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.15|P-value:5.24E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:19.00|P-value:2.71E-89 ID:SGTC_582|Compound:0987-0078|FD-Score:5.99|P-value:1.20E-10 ID:SGTC_601|Compound:0986-0029|FD-Score:6.00|P-value:1.11E-10 ID:SGTC_609|Compound:0986-0026|FD-Score:8.52|P-value:1.23E-19 ID:SGTC_645|Compound:0845-0943|FD-Score:4.76|P-value:2.37E-7 ID:SGTC_658|Compound:3253-0951|FD-Score:3.22|P-value:3.13E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.22|P-value:3.18E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:3.89|P-value:1.83E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:8.96|P-value:1.62E-21 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.24|P-value:3.64E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-3.01|P-value:8.55E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.18|P-value:3.73E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:2.98|P-value:7.67E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:4.80|P-value:1.87E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:19.00|P-value:1.58E-89 ID:SGTC_1690|Compound:st023502|FD-Score:3.43|P-value:1.35E-4 ID:SGTC_1712|Compound:st032288|FD-Score:15.40|P-value:2.48E-59 ID:SGTC_1726|Compound:st035556|FD-Score:4.71|P-value:3.16E-7 ID:SGTC_1733|Compound:st037357|FD-Score:5.47|P-value:3.54E-9 ID:SGTC_1754|Compound:st042065|FD-Score:3.46|P-value:1.21E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.19|P-value:3.48E-4 ID:SGTC_2298|Compound:7976326|FD-Score:3.26|P-value:2.67E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.10|P-value:5.00E-4 ID:SGTC_2337|Compound:9009472|FD-Score:2.91|P-value:9.86E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:-3.16|P-value:5.05E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.17|P-value:3.76E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.10|P-value:6.83E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:3.40|P-value:1.55E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.36|P-value:1.93E-6 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.49|P-value:1.02E-6 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.41|P-value:1.48E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.00|P-value:7.31E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.67|P-value:2.80E-16 ID:SGTC_2710|Compound:st078562|FD-Score:3.17|P-value:3.76E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.83|P-value:2.47E-5 ID:SGTC_2780|Compound:5215298|FD-Score:2.93|P-value:9.15E-4 ID:SGTC_2803|Compound:7707263|FD-Score:-3.10|P-value:6.15E-4 ID:SGTC_2926|Compound:9010726|FD-Score:6.94|P-value:1.16E-13 ID:SGTC_3152|Compound:9099253|FD-Score:2.93|P-value:9.20E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-3.27|P-value:3.32E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:4.07|P-value:7.94E-6 ID:SGTC_40|Compound:0327-0103|FD-Score:5.37|P-value:6.71E-9 ID:SGTC_54|Compound:0159-0055|FD-Score:4.83|P-value:1.61E-7 ID:SGTC_57|Compound:miconazole|FD-Score:3.01|P-value:6.98E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:6.95|P-value:1.09E-13 ID:SGTC_187|Compound:1488-2104|FD-Score:23.60|P-value:2.32E-136 ID:SGTC_211|Compound:3455-0679|FD-Score:3.90|P-value:1.79E-5 ID:SGTC_298|Compound:1391-0729|FD-Score:5.45|P-value:4.03E-9 ID:SGTC_304|Compound:1310-0298|FD-Score:-2.99|P-value:9.42E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.13|P-value:5.52E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.16|P-value:5.20E-6 ID:SGTC_314|Compound:1671-0008|FD-Score:5.32|P-value:9.19E-9 ID:SGTC_383|Compound:2324-0395|FD-Score:3.57|P-value:7.54E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:16.80|P-value:3.84E-70 ID:SGTC_502|Compound:l-744,832|FD-Score:5.57|P-value:1.92E-9 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.15|P-value:5.24E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:19.00|P-value:2.71E-89 ID:SGTC_582|Compound:0987-0078|FD-Score:5.99|P-value:1.20E-10 ID:SGTC_601|Compound:0986-0029|FD-Score:6.00|P-value:1.11E-10 ID:SGTC_609|Compound:0986-0026|FD-Score:8.52|P-value:1.23E-19 ID:SGTC_645|Compound:0845-0943|FD-Score:4.76|P-value:2.37E-7 ID:SGTC_658|Compound:3253-0951|FD-Score:3.22|P-value:3.13E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.22|P-value:3.18E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:3.89|P-value:1.83E-5 ID:SGTC_902|Compound:1120-0002|FD-Score:8.96|P-value:1.62E-21 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.24|P-value:3.64E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-3.01|P-value:8.55E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.18|P-value:3.73E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:2.98|P-value:7.67E-4 ID:SGTC_1476|Compound:4112-3294|FD-Score:4.80|P-value:1.87E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:19.00|P-value:1.58E-89 ID:SGTC_1690|Compound:st023502|FD-Score:3.43|P-value:1.35E-4 ID:SGTC_1712|Compound:st032288|FD-Score:15.40|P-value:2.48E-59 ID:SGTC_1726|Compound:st035556|FD-Score:4.71|P-value:3.16E-7 ID:SGTC_1733|Compound:st037357|FD-Score:5.47|P-value:3.54E-9 ID:SGTC_1754|Compound:st042065|FD-Score:3.46|P-value:1.21E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.19|P-value:3.48E-4 ID:SGTC_2298|Compound:7976326|FD-Score:3.26|P-value:2.67E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.10|P-value:5.00E-4 ID:SGTC_2337|Compound:9009472|FD-Score:2.91|P-value:9.86E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:-3.16|P-value:5.05E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.17|P-value:3.76E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.10|P-value:6.83E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:3.40|P-value:1.55E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.36|P-value:1.93E-6 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.49|P-value:1.02E-6 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.41|P-value:1.48E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.00|P-value:7.31E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.67|P-value:2.80E-16 ID:SGTC_2710|Compound:st078562|FD-Score:3.17|P-value:3.76E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.83|P-value:2.47E-5 ID:SGTC_2780|Compound:5215298|FD-Score:2.93|P-value:9.15E-4 ID:SGTC_2803|Compound:7707263|FD-Score:-3.10|P-value:6.15E-4 ID:SGTC_2926|Compound:9010726|FD-Score:6.94|P-value:1.16E-13 ID:SGTC_3152|Compound:9099253|FD-Score:2.93|P-value:9.20E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-3.27|P-value:3.32E-4

Top fitness defect scores for YJL165C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_187 1488-2104 23.60 32.7 µM 2.32E-136 calcium & mitochondrial duress 1 3.61E-123
2 SGTC_1597 7-benzyloxygramine 19.00 47.5 µM 1.58E-89 calcium & mitochondrial duress 4 6.93E-81
3 SGTC_528 3492-0019 19.00 172.0 µM 2.71E-89 calcium & mitochondrial duress 2 1.13E-80
4 SGTC_467 ro 31-8220 16.80 72.8 µM 3.84E-70 calcium & mitochondrial duress 1 2.18E-63
5 SGTC_1712 st032288 15.40 53.4 µM 2.48E-59 calcium & mitochondrial duress 2 1.25E-53
6 SGTC_902 1120-0002 8.96 2.1 µM 1.62E-21 azole & statin 6 1.65E-19
7 SGTC_609 0986-0026 8.52 86.3 µM 1.23E-19 calcium & mitochondrial duress 2 8.17E-18
8 SGTC_2688 kanamycin b 7.67 96.7 µM 2.80E-16 calcium & mitochondrial duress 80 8.70E-15
9 SGTC_65 0798-0905 6.95 35.0 µM 1.09E-13 calcium & mitochondrial duress 21 1.89E-12
10 SGTC_2926 9010726 6.94 65.2 µM 1.16E-13 5 1.99E-12
11 SGTC_601 0986-0029 6.00 62.9 µM 1.11E-10 calcium & mitochondrial duress 9 9.76E-10
12 SGTC_582 0987-0078 5.99 156.0 µM 1.20E-10 calcium & mitochondrial duress 21 1.04E-9
13 SGTC_502 l-744,832 5.57 89.3 µM 1.92E-9 calcium & mitochondrial duress 12 1.27E-8
14 SGTC_1733 st037357 5.47 54.9 µM 3.54E-9 60S ribosome export 7 2.21E-8
15 SGTC_298 1391-0729 5.45 10.0 µM 4.03E-9 calcium & mitochondrial duress 6 2.48E-8
16 SGTC_40 0327-0103 5.37 19.8 µM 6.71E-9 9 3.93E-8
17 SGTC_314 1671-0008 5.32 2.7 µM 9.19E-9 14 5.22E-8
18 SGTC_54 0159-0055 4.83 9.0 µM 1.61E-7 ERG2 30 6.89E-7
19 SGTC_1476 4112-3294 4.80 3.3 µM 1.87E-7 60S ribosome export 7 7.89E-7
20 SGTC_645 0845-0943 4.76 5.4 µM 2.37E-7 60S ribosome export 2 9.77E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.558 4.67E-273 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.503 4.90E-214 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.495 1.78E-206 YOL016C CMK2 hom Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
0.470 2.63E-183 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.418 1.54E-141 YHR064C SSZ1 hom Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
0.381 6.49E-116 YBR109C CMD1 het Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
0.372 4.58E-110 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.358 6.82E-102 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.340 3.34E-91 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.336 2.43E-89 YCR008W SAT4 hom Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
0.331 4.00E-86 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.323 1.00E-81 YIL154C IMP2' hom Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
0.310 1.37E-75 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.288 6.47E-65 YDL160C DHH1 hom Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
0.287 1.79E-64 YNL288W CAF40 hom Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p