YJL173C / RFA3

Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL173C / RFA3

Click on Significant Values for Screen Details ID:SGTC_126|Compound:0417-1624|FD-Score:-3.21|P-value:4.80E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:3.47|P-value:1.82E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-9.76|P-value:5.64E-24 ID:SGTC_154|Compound:3389-1638|FD-Score:-4.17|P-value:8.85E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.75|P-value:5.77E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:5.82|P-value:1.09E-9 ID:SGTC_527|Compound:4476-4215|FD-Score:3.63|P-value:9.60E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:3.60|P-value:1.09E-4 ID:SGTC_703|Compound:3875-0075|FD-Score:4.49|P-value:1.99E-6 ID:SGTC_707|Compound:0865-0008|FD-Score:4.43|P-value:2.64E-6 ID:SGTC_743|Compound:1275-0778|FD-Score:3.52|P-value:1.50E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.31|P-value:3.32E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.42|P-value:2.22E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:6.75|P-value:2.00E-12 ID:SGTC_970|Compound:1315-0089|FD-Score:4.05|P-value:1.60E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.31|P-value:3.32E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.59|P-value:1.15E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.24|P-value:4.32E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:5.17|P-value:5.56E-8 ID:SGTC_1238|Compound:0333-0287|FD-Score:-3.26|P-value:4.03E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.52|P-value:1.52E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.22|P-value:4.78E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:4.09|P-value:1.36E-5 ID:SGTC_1339|Compound:1486-1321|FD-Score:-3.05|P-value:8.38E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.33|P-value:3.03E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-3.56|P-value:1.23E-4 ID:SGTC_2055|Compound:5235184|FD-Score:5.05|P-value:1.05E-7 ID:SGTC_2255|Compound:7941087|FD-Score:-4.07|P-value:1.45E-5 ID:SGTC_2273|Compound:7815444|FD-Score:3.89|P-value:3.18E-5 ID:SGTC_2299|Compound:7976368|FD-Score:4.44|P-value:2.53E-6 ID:SGTC_2315|Compound:7733312|FD-Score:-3.48|P-value:1.76E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.16|P-value:5.79E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-4.72|P-value:6.02E-7 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-5.32|P-value:2.24E-8 ID:SGTC_2583|Compound:asiatic acid|FD-Score:5.07|P-value:9.80E-8 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.76|P-value:5.61E-5 ID:SGTC_2816|Compound:7982706|FD-Score:-3.12|P-value:6.65E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-3.12|P-value:6.64E-4 ID:SGTC_2889|Compound:9059656|FD-Score:4.43|P-value:2.65E-6 ID:SGTC_2894|Compound:9048625|FD-Score:-3.49|P-value:1.68E-4 ID:SGTC_2925|Compound:9010536|FD-Score:4.32|P-value:4.61E-6 ID:SGTC_2959|Compound:9079155|FD-Score:3.10|P-value:7.36E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.62|P-value:1.02E-6 ID:SGTC_2967|Compound:9089538|FD-Score:4.08|P-value:1.42E-5 ID:SGTC_2985|Compound:9022375|FD-Score:-3.84|P-value:3.94E-5 ID:SGTC_3009|Compound:9080819|FD-Score:-3.26|P-value:4.04E-4 ID:SGTC_3010|Compound:9080089|FD-Score:-3.19|P-value:5.10E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.43|P-value:2.14E-4 ID:SGTC_3093|Compound:9117344|FD-Score:-4.13|P-value:1.07E-5 ID:SGTC_3140|Compound:9094243|FD-Score:3.04|P-value:9.07E-4 ID:SGTC_3235|Compound:9132602|FD-Score:3.24|P-value:4.37E-4 ID:SGTC_3253|Compound:9135987|FD-Score:-3.11|P-value:7.00E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-3.21|P-value:4.80E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:3.47|P-value:1.82E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-9.76|P-value:5.64E-24 ID:SGTC_154|Compound:3389-1638|FD-Score:-4.17|P-value:8.85E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.75|P-value:5.77E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:5.82|P-value:1.09E-9 ID:SGTC_527|Compound:4476-4215|FD-Score:3.63|P-value:9.60E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:3.60|P-value:1.09E-4 ID:SGTC_703|Compound:3875-0075|FD-Score:4.49|P-value:1.99E-6 ID:SGTC_707|Compound:0865-0008|FD-Score:4.43|P-value:2.64E-6 ID:SGTC_743|Compound:1275-0778|FD-Score:3.52|P-value:1.50E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.31|P-value:3.32E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-3.42|P-value:2.22E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:6.75|P-value:2.00E-12 ID:SGTC_970|Compound:1315-0089|FD-Score:4.05|P-value:1.60E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.31|P-value:3.32E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.59|P-value:1.15E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.24|P-value:4.32E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:5.17|P-value:5.56E-8 ID:SGTC_1238|Compound:0333-0287|FD-Score:-3.26|P-value:4.03E-4 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.52|P-value:1.52E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.22|P-value:4.78E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:4.09|P-value:1.36E-5 ID:SGTC_1339|Compound:1486-1321|FD-Score:-3.05|P-value:8.38E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.33|P-value:3.03E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-3.56|P-value:1.23E-4 ID:SGTC_2055|Compound:5235184|FD-Score:5.05|P-value:1.05E-7 ID:SGTC_2255|Compound:7941087|FD-Score:-4.07|P-value:1.45E-5 ID:SGTC_2273|Compound:7815444|FD-Score:3.89|P-value:3.18E-5 ID:SGTC_2299|Compound:7976368|FD-Score:4.44|P-value:2.53E-6 ID:SGTC_2315|Compound:7733312|FD-Score:-3.48|P-value:1.76E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.16|P-value:5.79E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-4.72|P-value:6.02E-7 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-5.32|P-value:2.24E-8 ID:SGTC_2583|Compound:asiatic acid|FD-Score:5.07|P-value:9.80E-8 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.76|P-value:5.61E-5 ID:SGTC_2816|Compound:7982706|FD-Score:-3.12|P-value:6.65E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-3.12|P-value:6.64E-4 ID:SGTC_2889|Compound:9059656|FD-Score:4.43|P-value:2.65E-6 ID:SGTC_2894|Compound:9048625|FD-Score:-3.49|P-value:1.68E-4 ID:SGTC_2925|Compound:9010536|FD-Score:4.32|P-value:4.61E-6 ID:SGTC_2959|Compound:9079155|FD-Score:3.10|P-value:7.36E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.62|P-value:1.02E-6 ID:SGTC_2967|Compound:9089538|FD-Score:4.08|P-value:1.42E-5 ID:SGTC_2985|Compound:9022375|FD-Score:-3.84|P-value:3.94E-5 ID:SGTC_3009|Compound:9080819|FD-Score:-3.26|P-value:4.04E-4 ID:SGTC_3010|Compound:9080089|FD-Score:-3.19|P-value:5.10E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.43|P-value:2.14E-4 ID:SGTC_3093|Compound:9117344|FD-Score:-4.13|P-value:1.07E-5 ID:SGTC_3140|Compound:9094243|FD-Score:3.04|P-value:9.07E-4 ID:SGTC_3235|Compound:9132602|FD-Score:3.24|P-value:4.37E-4 ID:SGTC_3253|Compound:9135987|FD-Score:-3.11|P-value:7.00E-4

Top fitness defect scores for YJL173C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_936 3005-4620 6.75 401.0 µM 2.00E-12 1 7.24E-12 1.99
2 SGTC_213 1246-0773 5.82 967.0 nM 1.09E-9 1 2.86E-9 1.84
3 SGTC_1227 3909-8010 5.17 46.6 µM 5.56E-8 1 1.19E-7 1.10
4 SGTC_2583 asiatic acid 5.07 100.0 µM 9.80E-8 3 2.03E-7 0.39
5 SGTC_2055 5235184 5.05 200.0 µM 1.05E-7 ERG2 2 2.18E-7 0.18
6 SGTC_703 3875-0075 4.49 71.8 µM 1.99E-6 2 3.54E-6 0.10
7 SGTC_2299 7976368 4.44 114.9 µM 2.53E-6 5 4.45E-6 0.45
8 SGTC_707 0865-0008 4.43 204.0 µM 2.64E-6 DNA damage response 4 4.64E-6 0.85
9 SGTC_2889 9059656 4.43 52.0 µM 2.65E-6 2 4.66E-6 0.93
10 SGTC_2925 9010536 4.32 32.5 µM 4.61E-6 azole & statin 4 7.87E-6 0.13
11 SGTC_1322 1348-1070 4.09 12.0 µM 1.36E-5 2 2.20E-5 0.60
12 SGTC_2967 9089538 4.08 49.9 µM 1.42E-5 13 2.30E-5 0.07
13 SGTC_970 1315-0089 4.05 19.5 µM 1.60E-5 RSC & ERG11 12 2.57E-5 0.32
14 SGTC_2273 7815444 3.89 169.1 µM 3.18E-5 3 4.93E-5 0.11
15 SGTC_2636 apigenin dimethyl ether 3.76 100.0 µM 5.61E-5 13 8.46E-5 0.10
16 SGTC_527 4476-4215 3.63 268.0 µM 9.60E-5 PDR1 6 1.41E-4 0.31
17 SGTC_530 4358-1714 3.60 28.0 µM 1.09E-4 10 1.59E-4 0.25
18 SGTC_1023 4482-3888 3.59 16.0 µM 1.15E-4 TSC3-RPN4 12 1.67E-4 0.12
19 SGTC_743 1275-0778 3.52 158.0 µM 1.50E-4 excess fatty acid 21 2.15E-4 0.21
20 SGTC_1278 0874-0585 3.52 172.0 µM 1.52E-4 13 2.18E-4 0.15

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.182 2.43E-26 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.154 2.25E-19 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.149 3.56E-18 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.141 2.14E-16 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.140 3.85E-16 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.131 2.26E-14 YIL147C SLN1 het Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
0.126 2.04E-13 YAL034W-A MTW1 het Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
0.124 6.73E-13 YKL036C_d YKL036C_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
0.122 1.56E-12 YFR031C SMC2 het Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
0.121 2.12E-12 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.118 7.16E-12 YML069W POB3 het Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
0.112 6.80E-11 YGR082W TOM20 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
0.112 8.19E-11 YGR280C PXR1 het Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
0.112 9.18E-11 YER159C BUR6 het Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
0.110 1.91E-10 YER118C SHO1 hom Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p