YJL194W / CDC6

Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL194W / CDC6

Click on Significant Values for Screen Details ID:SGTC_141|Compound:0443-0274|FD-Score:-5.64|P-value:2.81E-8 ID:SGTC_175|Compound:3793-3120|FD-Score:3.31|P-value:6.26E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.82|P-value:1.42E-6 ID:SGTC_193|Compound:1273-0060|FD-Score:3.25|P-value:7.82E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.71|P-value:1.56E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-6.25|P-value:8.60E-10 ID:SGTC_472|Compound:calcifediol|FD-Score:3.93|P-value:6.61E-5 ID:SGTC_496|Compound:calpeptin|FD-Score:5.88|P-value:5.77E-9 ID:SGTC_500|Compound:h-89|FD-Score:3.48|P-value:3.52E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.85|P-value:1.05E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.27|P-value:7.17E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:3.37|P-value:5.25E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.74|P-value:2.04E-6 ID:SGTC_796|Compound:1234-0020|FD-Score:5.50|P-value:4.66E-8 ID:SGTC_821|Compound:1114-0266|FD-Score:3.36|P-value:5.40E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.81|P-value:1.04E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.96|P-value:5.93E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.13|P-value:3.53E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.45|P-value:4.64E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-3.27|P-value:8.27E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:4.90|P-value:9.44E-7 ID:SGTC_1514|Compound:1189-1591|FD-Score:-3.33|P-value:6.95E-4 ID:SGTC_1780|Compound:st038448|FD-Score:4.03|P-value:4.37E-5 ID:SGTC_1851|Compound:st056236|FD-Score:-3.25|P-value:9.10E-4 ID:SGTC_1860|Compound:st056228|FD-Score:-3.46|P-value:4.41E-4 ID:SGTC_2033|Compound:5192550|FD-Score:3.23|P-value:8.19E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.29|P-value:7.86E-4 ID:SGTC_2280|Compound:7785178|FD-Score:4.76|P-value:1.89E-6 ID:SGTC_2301|Compound:7769476|FD-Score:3.40|P-value:4.71E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.39|P-value:4.80E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-4.33|P-value:1.56E-5 ID:SGTC_2839|Compound:9003951|FD-Score:3.54|P-value:2.88E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-3.65|P-value:2.23E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.25|P-value:7.67E-4 ID:SGTC_3032|Compound:9089427|FD-Score:3.50|P-value:3.30E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.64|P-value:2.81E-8 ID:SGTC_175|Compound:3793-3120|FD-Score:3.31|P-value:6.26E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.82|P-value:1.42E-6 ID:SGTC_193|Compound:1273-0060|FD-Score:3.25|P-value:7.82E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.71|P-value:1.56E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-6.25|P-value:8.60E-10 ID:SGTC_472|Compound:calcifediol|FD-Score:3.93|P-value:6.61E-5 ID:SGTC_496|Compound:calpeptin|FD-Score:5.88|P-value:5.77E-9 ID:SGTC_500|Compound:h-89|FD-Score:3.48|P-value:3.52E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.85|P-value:1.05E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.27|P-value:7.17E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:3.37|P-value:5.25E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.74|P-value:2.04E-6 ID:SGTC_796|Compound:1234-0020|FD-Score:5.50|P-value:4.66E-8 ID:SGTC_821|Compound:1114-0266|FD-Score:3.36|P-value:5.40E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.81|P-value:1.04E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.96|P-value:5.93E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-4.13|P-value:3.53E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.45|P-value:4.64E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-3.27|P-value:8.27E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:4.90|P-value:9.44E-7 ID:SGTC_1514|Compound:1189-1591|FD-Score:-3.33|P-value:6.95E-4 ID:SGTC_1780|Compound:st038448|FD-Score:4.03|P-value:4.37E-5 ID:SGTC_1851|Compound:st056236|FD-Score:-3.25|P-value:9.10E-4 ID:SGTC_1860|Compound:st056228|FD-Score:-3.46|P-value:4.41E-4 ID:SGTC_2033|Compound:5192550|FD-Score:3.23|P-value:8.19E-4 ID:SGTC_2107|Compound:5363541|FD-Score:-3.29|P-value:7.86E-4 ID:SGTC_2280|Compound:7785178|FD-Score:4.76|P-value:1.89E-6 ID:SGTC_2301|Compound:7769476|FD-Score:3.40|P-value:4.71E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.39|P-value:4.80E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-4.33|P-value:1.56E-5 ID:SGTC_2839|Compound:9003951|FD-Score:3.54|P-value:2.88E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-3.65|P-value:2.23E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.25|P-value:7.67E-4 ID:SGTC_3032|Compound:9089427|FD-Score:3.50|P-value:3.30E-4

Top fitness defect scores for YJL194W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_496 calpeptin 5.88 138.0 µM 5.77E-9 2 2.08E-9 1.51
2 SGTC_796 1234-0020 5.50 70.7 µM 4.66E-8 SWF1 & branched chain AA biosynthesis 1 1.91E-8 0.63
3 SGTC_1509 1326-1131 4.90 80.0 µM 9.44E-7 1 4.67E-7 0.91
4 SGTC_178 4092-0806 4.82 87.5 µM 1.42E-6 ergosterol depletion effects on membrane 16 7.21E-7 0.26
5 SGTC_2280 7785178 4.76 200.0 µM 1.89E-6 1 9.79E-7 0.85
6 SGTC_674 0498-0004 4.74 451.0 µM 2.04E-6 heme requiring 9 1.06E-6 0.06
7 SGTC_1780 st038448 4.03 24.5 µM 4.37E-5 iron homeostasis 7 2.73E-5 0.20
8 SGTC_1325 1431-0015 3.96 227.0 µM 5.93E-5 Golgi 3 3.78E-5 0.36
9 SGTC_472 calcifediol 3.93 10.0 µM 6.61E-5 1 4.24E-5 0.52
10 SGTC_1122 capsaicin 3.81 818.6 nM 1.04E-4 5 6.83E-5 0.20
11 SGTC_353 1988-1175 3.71 58.3 µM 1.56E-4 unfolded protein response 9 1.05E-4 0.14
12 SGTC_2839 9003951 3.54 26.0 µM 2.88E-4 4 2.02E-4 0.01
13 SGTC_3032 9089427 3.50 49.5 µM 3.30E-4 60S ribosome export 7 2.33E-4 0.29
14 SGTC_500 h-89 3.48 112.0 µM 3.52E-4 cell wall signaling 14 2.50E-4 0.02
15 SGTC_2301 7769476 3.40 200.0 µM 4.71E-4 19 3.40E-4 0.01
16 SGTC_2539 epi(13)torulosol 3.39 80.0 µM 4.80E-4 9 3.47E-4 0.11
17 SGTC_664 1222-0054 3.37 14.9 µM 5.25E-4 sphingolipid biosynthesis & PDR1 14 3.81E-4 0.09
18 SGTC_821 1114-0266 3.36 29.2 µM 5.40E-4 7 3.93E-4 0.02
19 SGTC_175 3793-3120 3.31 23.7 µM 6.26E-4 13 4.59E-4 0.14
20 SGTC_651 3253-0966 3.27 34.4 µM 7.17E-4 calcium & mitochondrial duress 9 5.30E-4 0.11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 3.35E-11 YFR031C SMC2 het Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
0.105 1.20E-9 YML098W TAF13 het TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
0.101 5.38E-9 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.095 3.23E-8 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.095 3.90E-8 YFL012W_p YFL012W_p hom Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin
0.092 8.72E-8 YIL147C SLN1 het Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
0.089 2.81E-7 YBL018C POP8 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.088 3.69E-7 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.087 4.12E-7 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.085 7.58E-7 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.084 1.01E-6 YGL120C PRP43 het RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
0.084 1.26E-6 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.082 1.78E-6 YMR141C_d YMR141C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082 1.92E-6 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.081 2.70E-6 YJL175W_d YJL175W_d hom Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor