YJL208C / NUC1

Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL208C / NUC1

Click on Significant Values for Screen Details ID:SGTC_149|Compound:3970-0822|FD-Score:-3.44|P-value:2.28E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:-3.40|P-value:2.67E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.08|P-value:8.35E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.52|P-value:1.67E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:4.98|P-value:2.30E-7 ID:SGTC_523|Compound:1934-0222|FD-Score:4.41|P-value:4.10E-6 ID:SGTC_621|Compound:3584-0052|FD-Score:-3.10|P-value:7.95E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-4.26|P-value:7.11E-6 ID:SGTC_766|Compound:1391-0721|FD-Score:3.94|P-value:3.33E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.70|P-value:9.12E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.50|P-value:2.01E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.19|P-value:6.44E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.47|P-value:2.24E-4 ID:SGTC_1644|Compound:st010134|FD-Score:3.63|P-value:1.19E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.20|P-value:5.48E-4 ID:SGTC_1683|Compound:st010134|FD-Score:-4.14|P-value:1.23E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-4.12|P-value:1.38E-5 ID:SGTC_2020|Compound:4017018|FD-Score:-3.19|P-value:5.72E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.89|P-value:4.12E-5 ID:SGTC_2067|Compound:5224629|FD-Score:-5.53|P-value:9.20E-9 ID:SGTC_2069|Compound:5228469|FD-Score:-4.40|P-value:3.84E-6 ID:SGTC_2190|Compound:6049624|FD-Score:-3.12|P-value:7.49E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.50|P-value:2.05E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.12|P-value:7.36E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.32|P-value:3.62E-4 ID:SGTC_2302|Compound:7771753|FD-Score:6.02|P-value:5.36E-10 ID:SGTC_2402|Compound:5553605|FD-Score:3.34|P-value:3.63E-4 ID:SGTC_2439|Compound:5492030|FD-Score:5.61|P-value:6.63E-9 ID:SGTC_2444|Compound:5485484|FD-Score:3.23|P-value:5.58E-4 ID:SGTC_2446|Compound:5162541|FD-Score:-4.12|P-value:1.35E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:3.09|P-value:9.06E-4 ID:SGTC_2692|Compound:st077426|FD-Score:4.13|P-value:1.45E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.10|P-value:7.96E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.44|P-value:2.28E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:-3.40|P-value:2.67E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.08|P-value:8.35E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.52|P-value:1.67E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:4.98|P-value:2.30E-7 ID:SGTC_523|Compound:1934-0222|FD-Score:4.41|P-value:4.10E-6 ID:SGTC_621|Compound:3584-0052|FD-Score:-3.10|P-value:7.95E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-4.26|P-value:7.11E-6 ID:SGTC_766|Compound:1391-0721|FD-Score:3.94|P-value:3.33E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.70|P-value:9.12E-5 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.50|P-value:2.01E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.19|P-value:6.44E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.47|P-value:2.24E-4 ID:SGTC_1644|Compound:st010134|FD-Score:3.63|P-value:1.19E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-3.20|P-value:5.48E-4 ID:SGTC_1683|Compound:st010134|FD-Score:-4.14|P-value:1.23E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-4.12|P-value:1.38E-5 ID:SGTC_2020|Compound:4017018|FD-Score:-3.19|P-value:5.72E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.89|P-value:4.12E-5 ID:SGTC_2067|Compound:5224629|FD-Score:-5.53|P-value:9.20E-9 ID:SGTC_2069|Compound:5228469|FD-Score:-4.40|P-value:3.84E-6 ID:SGTC_2190|Compound:6049624|FD-Score:-3.12|P-value:7.49E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.50|P-value:2.05E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.12|P-value:7.36E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:-3.32|P-value:3.62E-4 ID:SGTC_2302|Compound:7771753|FD-Score:6.02|P-value:5.36E-10 ID:SGTC_2402|Compound:5553605|FD-Score:3.34|P-value:3.63E-4 ID:SGTC_2439|Compound:5492030|FD-Score:5.61|P-value:6.63E-9 ID:SGTC_2444|Compound:5485484|FD-Score:3.23|P-value:5.58E-4 ID:SGTC_2446|Compound:5162541|FD-Score:-4.12|P-value:1.35E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:3.09|P-value:9.06E-4 ID:SGTC_2692|Compound:st077426|FD-Score:4.13|P-value:1.45E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.10|P-value:7.96E-4

Top fitness defect scores for YJL208C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2302 7771753 6.02 200.0 µM 5.36E-10 13 8.55E-10
2 SGTC_2439 5492030 5.61 7.2 µM 6.63E-9 4 9.91E-9
3 SGTC_359 0081-0058 4.98 15.9 µM 2.30E-7 60S ribosome export 19 3.14E-7
4 SGTC_523 1934-0222 4.41 13.7 µM 4.10E-6 24 5.21E-6
5 SGTC_2692 st077426 4.13 47.7 µM 1.45E-5 20 1.79E-5
6 SGTC_766 1391-0721 3.94 126.0 µM 3.33E-5 14 4.01E-5
7 SGTC_2063 5246145 3.89 200.0 µM 4.12E-5 25 4.94E-5
8 SGTC_1047 2043-3044 3.70 82.0 µM 9.12E-5 45 1.07E-4
9 SGTC_1644 st010134 3.63 20.9 µM 1.19E-4 mitochondrial processes 17 1.39E-4
10 SGTC_1338 1486-1223 3.50 76.0 µM 2.01E-4 35 2.32E-4
11 SGTC_2200 6600604 3.50 21.9 µM 2.05E-4 tubulin folding & SWR complex 64 2.36E-4
12 SGTC_1611 st001856 3.47 99.4 µM 2.24E-4 30 2.58E-4
13 SGTC_2402 5553605 3.34 200.0 µM 3.63E-4 59 4.13E-4
14 SGTC_2444 5485484 3.23 36.4 µM 5.58E-4 63 6.28E-4
15 SGTC_1567 st069303 3.19 68.4 µM 6.44E-4 mitochondrial processes 48 7.23E-4
16 SGTC_2619 obliquin 3.09 87.9 µM 9.06E-4 cell wall 210 0.00101
17 SGTC_3319 9138450 3.05 16.8 µM 0.00104 51 0.00116
18 SGTC_1487 3970-0795 3.00 2.8 µM 0.00122 DNA intercalators 50 0.00135
19 SGTC_42 0133-0086 2.98 60.6 µM 0.00130 71 0.00144
20 SGTC_2757 clomiphene 2.96 4.2 µM 0.00139 fatty acid desaturase (OLE1) 100 0.00153

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.144 4.07E-17 YHL032C GUT1 hom Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
0.122 1.51E-12 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.120 2.52E-12 YMR148W OSW5 hom Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion
0.117 1.07E-11 YOR149C SMP3 het Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
0.109 2.37E-10 YIL165C_p YIL165C_p hom Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene
0.108 3.76E-10 YJR010W MET3 hom ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism
0.108 3.76E-10 YBL056W PTC3 hom Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication
0.103 2.04E-9 YEL066W HPA3 hom D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro
0.101 5.04E-9 YER081W SER3 hom 3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication
0.099 8.11E-9 YMR009W ADI1 hom Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions
0.097 2.02E-8 YJR017C ESS1 het Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
0.096 2.86E-8 YGR175C ERG1 het Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
0.095 3.70E-8 YDR538W PAD1 hom Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX
0.092 9.65E-8 YOR171C LCB4 hom Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes
0.092 1.06E-7 YPL134C ODC1 hom Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation