YJR010W / MET3

ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR010W / MET3

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:-2.97|P-value:7.93E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:3.04|P-value:8.46E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:-3.08|P-value:5.31E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:4.51|P-value:1.40E-6 ID:SGTC_78|Compound:0336-0123|FD-Score:-3.25|P-value:2.86E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.62|P-value:8.77E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.17|P-value:5.23E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.41|P-value:2.07E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.50|P-value:1.05E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:3.01|P-value:9.27E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.80|P-value:2.91E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.77|P-value:4.73E-5 ID:SGTC_1006|Compound:4099-6547|FD-Score:-3.05|P-value:6.00E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.05|P-value:8.24E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.15|P-value:5.63E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:4.04|P-value:1.43E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.37|P-value:1.73E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:-2.92|P-value:9.48E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.53|P-value:1.28E-6 ID:SGTC_1594|Compound:piperine|FD-Score:-3.32|P-value:2.11E-4 ID:SGTC_1642|Compound:st009582|FD-Score:3.06|P-value:7.86E-4 ID:SGTC_1722|Compound:st034304|FD-Score:-3.05|P-value:6.04E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.42|P-value:2.00E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-4.86|P-value:1.39E-7 ID:SGTC_2140|Compound:5586273|FD-Score:3.03|P-value:8.63E-4 ID:SGTC_2164|Compound:5685496|FD-Score:3.91|P-value:2.52E-5 ID:SGTC_2167|Compound:5876490|FD-Score:3.25|P-value:3.91E-4 ID:SGTC_2439|Compound:5492030|FD-Score:4.12|P-value:9.82E-6 ID:SGTC_2548|Compound:3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin|FD-Score:3.43|P-value:1.90E-4 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:2.99|P-value:9.98E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:3.13|P-value:6.01E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.05|P-value:6.10E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.30|P-value:3.24E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:4.66|P-value:6.43E-7 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.50|P-value:1.02E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.22|P-value:3.12E-4 ID:SGTC_2931|Compound:9014674|FD-Score:3.00|P-value:9.66E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.09|P-value:7.02E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.62|P-value:8.83E-5 ID:SGTC_2970|Compound:9090938|FD-Score:4.80|P-value:3.16E-7 ID:SGTC_3210|Compound:9128157|FD-Score:7.95|P-value:5.84E-17 ID:SGTC_3299|Compound:9121982|FD-Score:3.10|P-value:6.88E-4 ID:SGTC_3308|Compound:9128332|FD-Score:4.38|P-value:2.80E-6 ID:SGTC_3315|Compound:9136395|FD-Score:7.35|P-value:9.30E-15 ID:SGTC_3317|Compound:9138495|FD-Score:-3.05|P-value:6.03E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:-2.97|P-value:7.93E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:3.04|P-value:8.46E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:-3.08|P-value:5.31E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:4.51|P-value:1.40E-6 ID:SGTC_78|Compound:0336-0123|FD-Score:-3.25|P-value:2.86E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.62|P-value:8.77E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.17|P-value:5.23E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.41|P-value:2.07E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.50|P-value:1.05E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:3.01|P-value:9.27E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.80|P-value:2.91E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.77|P-value:4.73E-5 ID:SGTC_1006|Compound:4099-6547|FD-Score:-3.05|P-value:6.00E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.05|P-value:8.24E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:3.15|P-value:5.63E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:4.04|P-value:1.43E-5 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.37|P-value:1.73E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:-2.92|P-value:9.48E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.53|P-value:1.28E-6 ID:SGTC_1594|Compound:piperine|FD-Score:-3.32|P-value:2.11E-4 ID:SGTC_1642|Compound:st009582|FD-Score:3.06|P-value:7.86E-4 ID:SGTC_1722|Compound:st034304|FD-Score:-3.05|P-value:6.04E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.42|P-value:2.00E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-4.86|P-value:1.39E-7 ID:SGTC_2140|Compound:5586273|FD-Score:3.03|P-value:8.63E-4 ID:SGTC_2164|Compound:5685496|FD-Score:3.91|P-value:2.52E-5 ID:SGTC_2167|Compound:5876490|FD-Score:3.25|P-value:3.91E-4 ID:SGTC_2439|Compound:5492030|FD-Score:4.12|P-value:9.82E-6 ID:SGTC_2548|Compound:3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin|FD-Score:3.43|P-value:1.90E-4 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:2.99|P-value:9.98E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:3.13|P-value:6.01E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.05|P-value:6.10E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.30|P-value:3.24E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:4.66|P-value:6.43E-7 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.50|P-value:1.02E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.22|P-value:3.12E-4 ID:SGTC_2931|Compound:9014674|FD-Score:3.00|P-value:9.66E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.09|P-value:7.02E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.62|P-value:8.83E-5 ID:SGTC_2970|Compound:9090938|FD-Score:4.80|P-value:3.16E-7 ID:SGTC_3210|Compound:9128157|FD-Score:7.95|P-value:5.84E-17 ID:SGTC_3299|Compound:9121982|FD-Score:3.10|P-value:6.88E-4 ID:SGTC_3308|Compound:9128332|FD-Score:4.38|P-value:2.80E-6 ID:SGTC_3315|Compound:9136395|FD-Score:7.35|P-value:9.30E-15 ID:SGTC_3317|Compound:9138495|FD-Score:-3.05|P-value:6.03E-4

Top fitness defect scores for YJR010W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3210 9128157 7.95 49.5 µM 5.84E-17 6 9.22E-16
2 SGTC_3315 9136395 7.35 68.5 µM 9.30E-15 1 9.65E-14
3 SGTC_2970 9090938 4.80 41.9 µM 3.16E-7 RNA processing & uracil transport 14 8.06E-7
4 SGTC_2749 bexarotene 4.66 57.4 µM 6.43E-7 13 1.55E-6
5 SGTC_1456 k015-0012 4.53 13.0 µM 1.28E-6 22 2.93E-6
6 SGTC_73 0327-0325 4.51 16.2 µM 1.40E-6 DNA damage response 34 3.18E-6
7 SGTC_3308 9128332 4.38 17.6 µM 2.80E-6 plasma membrane duress 64 6.04E-6
8 SGTC_2439 5492030 4.12 7.2 µM 9.82E-6 9 1.92E-5
9 SGTC_1271 0832-2808 4.04 169.0 µM 1.43E-5 9 2.71E-5
10 SGTC_2164 5685496 3.91 200.0 µM 2.52E-5 24 4.59E-5
11 SGTC_932 2857-0803 3.77 154.0 µM 4.73E-5 30 8.21E-5
12 SGTC_142 0335-0849 3.62 62.7 µM 8.77E-5 ubiquinone biosynthesis & proteasome 147 1.45E-4
13 SGTC_2967 9089538 3.62 49.9 µM 8.83E-5 45 1.46E-4
14 SGTC_2548 3',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin 3.43 19.9 µM 1.90E-4 redox potentiating 19 2.97E-4
15 SGTC_1935 st074532 3.42 41.6 µM 2.00E-4 60 3.13E-4
16 SGTC_266 formosanin C 3.41 406.0 nM 2.07E-4 sphingolipid biosynthesis & PDR1 83 3.23E-4
17 SGTC_2584 androsterone 3.30 48.7 µM 3.24E-4 cell wall 175 4.88E-4
18 SGTC_2167 5876490 3.25 200.0 µM 3.91E-4 64 5.80E-4
19 SGTC_255 tunicamycin 3.17 200.5 nM 5.23E-4 unfolded protein response 120 7.60E-4
20 SGTC_1248 0469-0687 3.15 58.6 µM 5.63E-4 69 8.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.132 1.36E-14 YLL017W YLL017W hom Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
0.125 3.59E-13 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.122 1.08E-12 YDL079C MRK1 hom Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation
0.114 3.32E-11 YBR052C RFS1 hom Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
0.114 3.70E-11 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.110 1.48E-10 YGR035W-A_p YGR035W-A_p hom Putative protein of unknown function
0.110 1.74E-10 YLL006W-A_p YLL006W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.110 1.96E-10 YMR221C_p YMR221C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy
0.108 3.76E-10 YJL208C NUC1 hom Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy
0.105 8.83E-10 YJR055W HIT1 hom Protein of unknown function, required for growth at high temperature
0.101 4.92E-9 YMR020W FMS1 hom Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
0.098 1.21E-8 YDR057W YOS9 hom ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family
0.097 1.75E-8 YPR002W PDH1 hom Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
0.095 3.34E-8 YLR196W PWP1 het Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
0.093 6.02E-8 YER027C GAL83 hom One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain