YJR016C / ILV3

Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJR016C / ILV3

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:4.03|P-value:5.95E-6 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:2.91|P-value:7.84E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.76|P-value:2.10E-5 ID:SGTC_106|Compound:0092-0003|FD-Score:-2.98|P-value:5.91E-4 ID:SGTC_107|Compound:0358-0022|FD-Score:-3.21|P-value:2.35E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.46|P-value:8.23E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:3.49|P-value:7.21E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-2.90|P-value:7.95E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:2.85|P-value:9.70E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:-3.20|P-value:2.43E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.09|P-value:3.92E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:2.85|P-value:9.79E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:4.40|P-value:8.35E-7 ID:SGTC_900|Compound:1112-0045|FD-Score:4.67|P-value:1.91E-7 ID:SGTC_939|Compound:1165-0479|FD-Score:2.96|P-value:6.48E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.89|P-value:1.15E-5 ID:SGTC_947|Compound:3447-0010|FD-Score:-5.95|P-value:4.81E-11 ID:SGTC_1006|Compound:4099-6547|FD-Score:-3.97|P-value:7.75E-6 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.35|P-value:1.34E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.24|P-value:2.14E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-2.95|P-value:6.67E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.06|P-value:4.43E-4 ID:SGTC_1755|Compound:st041960|FD-Score:3.01|P-value:5.23E-4 ID:SGTC_2001|Compound:4023502|FD-Score:-2.99|P-value:5.60E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.93|P-value:7.18E-4 ID:SGTC_2335|Compound:9018011|FD-Score:3.08|P-value:4.03E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:2.95|P-value:6.65E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.05|P-value:4.62E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.63|P-value:3.91E-5 ID:SGTC_2891|Compound:9063876|FD-Score:2.90|P-value:8.14E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-6.31|P-value:3.22E-12 ID:SGTC_23|Compound:0213-0011|FD-Score:4.03|P-value:5.95E-6 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:2.91|P-value:7.84E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.76|P-value:2.10E-5 ID:SGTC_106|Compound:0092-0003|FD-Score:-2.98|P-value:5.91E-4 ID:SGTC_107|Compound:0358-0022|FD-Score:-3.21|P-value:2.35E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.46|P-value:8.23E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:3.49|P-value:7.21E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-2.90|P-value:7.95E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:2.85|P-value:9.70E-4 ID:SGTC_508|Compound:roscovitine|FD-Score:-3.20|P-value:2.43E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.09|P-value:3.92E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:2.85|P-value:9.79E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:4.40|P-value:8.35E-7 ID:SGTC_900|Compound:1112-0045|FD-Score:4.67|P-value:1.91E-7 ID:SGTC_939|Compound:1165-0479|FD-Score:2.96|P-value:6.48E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.89|P-value:1.15E-5 ID:SGTC_947|Compound:3447-0010|FD-Score:-5.95|P-value:4.81E-11 ID:SGTC_1006|Compound:4099-6547|FD-Score:-3.97|P-value:7.75E-6 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.35|P-value:1.34E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.24|P-value:2.14E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-2.95|P-value:6.67E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.06|P-value:4.43E-4 ID:SGTC_1755|Compound:st041960|FD-Score:3.01|P-value:5.23E-4 ID:SGTC_2001|Compound:4023502|FD-Score:-2.99|P-value:5.60E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.93|P-value:7.18E-4 ID:SGTC_2335|Compound:9018011|FD-Score:3.08|P-value:4.03E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:2.95|P-value:6.65E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.05|P-value:4.62E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.63|P-value:3.91E-5 ID:SGTC_2891|Compound:9063876|FD-Score:2.90|P-value:8.14E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-6.31|P-value:3.22E-12

Top fitness defect scores for YJR016C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_900 1112-0045 4.67 141.0 µM 1.91E-7 NEO1 4 1.52E-6 0.45
2 SGTC_884 0851-0618 4.40 334.0 µM 8.35E-7 4 5.32E-6 0.66
3 SGTC_23 0213-0011 4.03 40.0 µM 5.95E-6 copper-dependent oxidative stress 4 2.83E-5 0.30
4 SGTC_942 3253-1115 3.89 227.0 µM 1.15E-5 5 4.98E-5 0.03
5 SGTC_2794 7785382 3.63 27.3 µM 3.91E-5 17 1.41E-4 0.07
6 SGTC_119 0121-0043 3.49 24.8 µM 7.21E-5 mitochondrial stress 32 2.37E-4 0.02
7 SGTC_114 0364-0001 3.46 490.3 µM 8.23E-5 10 2.66E-4 0.03
8 SGTC_1176 0848-0131 3.24 83.3 µM 2.14E-4 Golgi 9 6.00E-4 0.07
9 SGTC_622 1348-1566 3.09 26.3 µM 3.92E-4 11 0.00101 0.00
10 SGTC_2335 9018011 3.08 154.2 µM 4.03E-4 20 0.00103 0.05
11 SGTC_1664 st014217 3.06 9.0 µM 4.43E-4 60S ribosome export 15 0.00112 0.07
12 SGTC_2505 galangin 3.05 7.9 µM 4.62E-4 RPP1 & pyrimidine depletion 12 0.00116 0.00
13 SGTC_1755 st041960 3.01 45.2 µM 5.23E-4 17 0.00129 0.11
14 SGTC_939 1165-0479 2.96 45.3 µM 6.48E-4 18 0.00155 0.03
15 SGTC_2491 paeonol 2.95 100.0 µM 6.65E-4 4 0.00158 0.08
16 SGTC_2115 tiliquinol 2.93 12.8 µM 7.18E-4 13 0.00169 0.01
17 SGTC_28 pentachloropyridine N-oxide 2.91 1.6 µM 7.84E-4 TSC3-RPN4 9 0.00182 0.03
18 SGTC_2891 9063876 2.90 19.5 µM 8.14E-4 6 0.00188 0.08
19 SGTC_439 amiodarone 2.85 34.4 µM 9.70E-4 NEO1-PIK1 20 0.00219 0.01
20 SGTC_649 1123-0311 2.85 367.0 µM 9.79E-4 endomembrane recycling 28 0.00221 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.163 2.69E-21 YER157W COG3 het Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.145 3.40E-17 YGL232W TAN1 hom Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
0.122 1.47E-12 YNL245C CWC25 het Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
0.117 1.14E-11 YBR043C QDR3 hom Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin
0.115 2.33E-11 YFL021W GAT1 hom Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p
0.115 2.52E-11 YLR444C_d YLR444C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.115 2.89E-11 YKL010C UFD4 hom Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress
0.112 7.70E-11 YFR027W ECO1 het Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
0.111 1.27E-10 YMR074C YMR074C hom Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
0.108 2.91E-10 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.107 4.31E-10 YOR245C DGA1 hom Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
0.105 1.16E-9 YPR132W RPS23B hom Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
0.103 2.16E-9 YGR209C TRX2 hom Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
0.102 3.07E-9 YFL009W CDC4 het F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
0.102 3.20E-9 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments